(Linear) (Six-base) MAPSORT of: KIEE2498.seq Check: 7366 from: 1 to: 13164 KIEE2498.orf With 182 enzymes: SgfI * March 20, 2014 14:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 885 4257 6618 12954 13164 Size: 885 3372 2361 6336 210 Fragments arranged by size: 6336 3372 2361 885 210 AatII G_ACGT'C Cuts at: 0 9770 13164 Size: 9770 3394 AccI GT'mk_AC Cuts at: 0 1465 1761 3472 8564 13164 Size: 1465 296 1711 5092 4600 Fragments arranged by size: 5092 4600 1711 1465 296 Acc65I G'GTAC_C Cuts at: 0 2944 11653 12407 13164 Size: 2944 8709 754 757 Fragments arranged by size: 8709 2944 757 754 AclI AA'CG_TT Cuts at: 0 1005 2558 3686 13164 Size: 1005 1553 1128 9478 Fragments arranged by size: 9478 1553 1128 1005 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 1421 2030 2059 2294 2579 3002 3283 Size: 181 1240 609 29 235 285 423 281 Cuts at: 3283 3432 3821 3877 4361 4864 5584 6212 6373 Size: 149 389 56 484 503 720 628 161 Cuts at: 6373 8059 8194 8252 8389 9870 10643 11246 12266 Size: 1686 135 58 137 1481 773 603 1020 Cuts at: 12266 13164 Size: 898 Fragments arranged by size: 1686 1481 1240 1020 898 773 720 628 609 603 503 484 423 389 285 281 235 181 161 149 137 135 58 56 29 AfeI AGC'GCT Cuts at: 0 6052 13164 Size: 6052 7112 AflII C'TTAA_G Cuts at: 0 12025 13164 Size: 12025 1139 AflIII A'CryG_T Cuts at: 0 1460 2538 13164 Size: 1460 1078 10626 Fragments arranged by size: 10626 1460 1078 AhdI GACnn_n'nnGTC Cuts at: 0 8490 13164 Size: 8490 4674 AleI CACnn'nnGTG Cuts at: 0 7483 8416 13164 Size: 7483 933 4748 Fragments arranged by size: 7483 4748 933 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1300 7501 7808 13164 Size: 1300 6201 307 5356 Fragments arranged by size: 6201 5356 1300 307 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1683 1715 10266 10298 10598 10630 13164 Size: 1683 32 8551 32 300 32 2534 Fragments arranged by size: 8551 2534 1683 300 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 2272 4739 7941 8083 8956 9913 11071 12714 Size: 2272 2467 3202 142 873 957 1158 1643 Cuts at: 12714 12824 13164 Size: 110 340 Fragments arranged by size: 3202 2467 2272 1643 1158 957 873 340 142 110 ApaI G_GGCC'C Cuts at: 0 3120 4917 12707 13164 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1218 839 595 231 BlpI GC'TnA_GC Cuts at: 0 11921 13164 Size: 11921 1243 Bme1580I G_kGCm'C Cuts at: 0 1417 3120 3274 4917 6371 8695 9126 11579 Size: 1417 1703 154 1643 1454 2324 431 2453 Cuts at: 11579 11766 12707 13164 Size: 187 941 457 Fragments arranged by size: 2453 2324 1703 1643 1454 1417 941 457 431 187 154 BmgBI CAC'GTC Cuts at: 0 2526 11135 13164 Size: 2526 8609 2029 Fragments arranged by size: 8609 2526 2029 BmrI ACTGGGnnnn_n' Cuts at: 0 649 2277 3114 4305 6365 7244 8944 11747 Size: 649 1628 837 1191 2060 879 1700 2803 Cuts at: 11747 13164 Size: 1417 Fragments arranged by size: 2803 2060 1700 1628 1417 1191 879 837 649 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2466 2638 5555 5780 6613 7845 8755 9414 Size: 2466 172 2917 225 833 1232 910 659 Cuts at: 9414 10998 13164 Size: 1584 2166 Fragments arranged by size: 2917 2466 2166 1584 1232 910 833 659 225 172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 231 370 736 832 1223 1282 2226 2405 Size: 231 139 366 96 391 59 944 179 Cuts at: 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1463 288 BsaBI GATnn'nnATC Cuts at: 0 241 4216 10009 13164 Size: 241 3975 5793 3155 Fragments arranged by size: 5793 3975 3155 241 BsaHI Gr'CG_yC Cuts at: 0 6003 9767 12596 13164 Size: 6003 3764 2829 568 Fragments arranged by size: 6003 3764 2829 568 BsaWI w'CCGG_w Cuts at: 0 1649 5420 5876 5906 8900 9143 9797 10616 Size: 1649 3771 456 30 2994 243 654 819 Cuts at: 10616 11387 13164 Size: 771 1777 Fragments arranged by size: 3771 2994 1777 1649 819 771 654 456 243 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 295 325 709 739 1102 1132 1213 1243 Size: 295 30 384 30 363 30 81 30 Cuts at: 1243 2238 2268 2322 2352 3895 3925 5271 5301 Size: 995 30 54 30 1543 30 1346 30 Cuts at: 5301 6050 6080 7693 7723 8537 8567 10598 10628 Size: 749 30 1613 30 814 30 2031 30 Cuts at: 10628 11213 11243 12860 12890 13164 Size: 585 30 1617 30 274 Fragments arranged by size: 2031 1617 1613 1543 1346 995 814 749 585 384 363 295 274 81 54 30 30 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 367 1435 1541 2119 2689 2776 2851 4689 Size: 367 1068 106 578 570 87 75 1838 Cuts at: 4689 4709 5090 6298 6687 8351 9943 10511 10532 Size: 20 381 1208 389 1664 1592 568 21 Cuts at: 10532 10971 11827 11839 12160 12828 13164 Size: 439 856 12 321 668 336 Fragments arranged by size: 1838 1664 1592 1208 1068 856 668 578 570 568 439 389 381 367 336 321 106 87 75 21 20 12 BseYI C'CCAG_C Cuts at: 0 1797 6047 6919 7039 7941 8288 8933 9675 Size: 1797 4250 872 120 902 347 645 742 Cuts at: 9675 11721 13164 Size: 2046 1443 Fragments arranged by size: 4250 2046 1797 1443 902 872 742 645 347 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2660 5539 5941 8720 9984 12681 12982 13164 Size: 2660 2879 402 2779 1264 2697 301 182 Fragments arranged by size: 2879 2779 2697 2660 1264 402 301 182 BsiEI CG_ry'CG Cuts at: 0 3180 13164 Size: 3180 9984 BsiHKAI G_wGCw'C Cuts at: 0 2431 2624 2933 6090 7490 8280 8609 8695 Size: 2431 193 309 3157 1400 790 329 86 Cuts at: 8695 9126 10868 11364 11579 11766 12833 12988 13164 Size: 431 1742 496 215 187 1067 155 176 Fragments arranged by size: 3157 2431 1742 1400 1067 790 496 431 329 309 215 193 187 176 155 86 BsmI GAATG_Cn' Cuts at: 0 975 2219 5004 5549 7669 8031 9605 9758 Size: 975 1244 2785 545 2120 362 1574 153 Cuts at: 9758 10967 11972 13164 Size: 1209 1005 1192 Fragments arranged by size: 2785 2120 1574 1244 1209 1192 1005 975 545 362 153 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3343 4106 8356 13164 Size: 3343 763 4250 4808 Fragments arranged by size: 4808 4250 3343 763 Bsp1286I G_dGCh'C Cuts at: 0 270 1260 1417 2431 2624 2933 3120 3274 Size: 270 990 157 1014 193 309 187 154 Cuts at: 3274 4917 5082 5312 6090 6290 6371 7490 8280 Size: 1643 165 230 778 200 81 1119 790 Cuts at: 8280 8521 8609 8695 8950 9126 10868 10981 11364 Size: 241 88 86 255 176 1742 113 383 Cuts at: 11364 11579 11766 12707 12833 12988 13164 Size: 215 187 941 126 155 176 Fragments arranged by size: 1742 1643 1119 1014 990 941 790 778 383 309 270 255 241 230 215 200 193 187 187 176 176 165 157 155 154 126 113 88 86 81 BspEI T'CCGG_A Cuts at: 0 5876 5906 8900 9797 10616 13164 Size: 5876 30 2994 897 819 2548 Fragments arranged by size: 5876 2994 2548 897 819 30 BspHI T'CATG_A Cuts at: 0 1176 4565 13164 Size: 1176 3389 8599 Fragments arranged by size: 8599 3389 1176 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 885 4257 4309 4620 6618 10189 12954 13164 Size: 885 3372 52 311 1998 3571 2765 210 Fragments arranged by size: 3571 3372 2765 1998 885 311 210 52 BsrBI CCG'CTC Cuts at: 0 11021 12393 13164 Size: 11021 1372 771 Fragments arranged by size: 11021 1372 771 BsrDI GCAATG_nn' Cuts at: 0 2916 5534 7763 10781 11700 13164 Size: 2916 2618 2229 3018 919 1464 Fragments arranged by size: 3018 2916 2618 2229 1464 919 BsrFI r'CCGG_y Cuts at: 0 848 2915 8648 11716 12442 13164 Size: 848 2067 5733 3068 726 722 Fragments arranged by size: 5733 3068 2067 848 726 722 BsrGI T'GTAC_A Cuts at: 0 564 4658 6842 11628 13164 Size: 564 4094 2184 4786 1536 Fragments arranged by size: 4786 4094 2184 1536 564 BssSI C'ACGA_G Cuts at: 0 1037 1449 4654 12987 13164 Size: 1037 412 3205 8333 177 Fragments arranged by size: 8333 3205 1037 412 177 BstAPI GCAn_nnn'nTGC Cuts at: 0 5010 5928 13164 Size: 5010 918 7236 Fragments arranged by size: 7236 5010 918 BstEII G'GTnAC_C Cuts at: 0 12966 13164 Size: 12966 198 BstXI CCAn_nnnn'nTGG Cuts at: 0 687 6999 9312 12588 13164 Size: 687 6312 2313 3276 576 Fragments arranged by size: 6312 3276 2313 687 576 BstYI r'GATC_y Cuts at: 0 1406 1806 2202 2598 3829 4496 4668 4899 Size: 1406 400 396 396 1231 667 172 231 Cuts at: 4899 5604 5625 6474 6552 6717 6807 6897 7147 Size: 705 21 849 78 165 90 90 250 Cuts at: 7147 7599 7902 8529 8612 9820 9830 10511 12021 Size: 452 303 627 83 1208 10 681 1510 Cuts at: 12021 12090 13164 Size: 69 1074 Fragments arranged by size: 1510 1406 1231 1208 1074 849 705 681 667 627 452 400 396 396 303 250 231 172 165 90 90 83 78 69 21 10 BstZ17I GTA'TAC Cuts at: 0 1466 13164 Size: 1466 11698 Bsu36I CC'TnA_GG Cuts at: 0 1968 2147 5543 13164 Size: 1968 179 3396 7621 Fragments arranged by size: 7621 3396 1968 179 BtgI C'CryG_G Cuts at: 0 674 3917 5585 5784 6684 6741 11252 12450 Size: 674 3243 1668 199 900 57 4511 1198 Cuts at: 12450 12945 13031 13164 Size: 495 86 133 Fragments arranged by size: 4511 3243 1668 1198 900 674 495 199 133 86 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 12783 13164 Size: 12783 381 BtsI GCAGTG_nn' Cuts at: 0 1284 1396 1436 3506 4386 5464 5510 6705 Size: 1284 112 40 2070 880 1078 46 1195 Cuts at: 6705 7805 9110 9126 11072 11747 12924 12999 13164 Size: 1100 1305 16 1946 675 1177 75 165 Fragments arranged by size: 2070 1946 1305 1284 1195 1177 1100 1078 880 675 165 112 75 46 40 16 ClaI AT'CG_AT Cuts at: 0 1182 3277 8147 13164 Size: 1182 2095 4870 5017 Fragments arranged by size: 5017 4870 2095 1182 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 413 448 2522 2557 13164 Size: 413 35 2074 35 10607 Fragments arranged by size: 10607 2074 413 35 35 DraI TTT'AAA Cuts at: 0 2877 5850 10907 13164 Size: 2877 2973 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124 423 Cuts at: 3002 3283 3432 3821 3877 4361 4510 4773 4864 Size: 281 149 389 56 484 149 263 91 Cuts at: 4864 5584 6212 6373 6463 6907 7411 7720 7840 Size: 720 628 161 90 444 504 309 120 Cuts at: 7840 8059 8194 8252 8279 8389 9197 9709 9870 Size: 219 135 58 27 110 808 512 161 Cuts at: 9870 10421 10643 11246 11350 12266 13164 Size: 551 222 603 104 916 898 Fragments arranged by size: 916 898 808 720 628 609 603 551 512 504 484 444 423 391 389 366 309 281 263 222 219 181 167 161 161 149 149 139 139 135 124 120 111 110 104 96 91 90 68 59 58 56 50 50 29 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 12175 13164 Size: 12175 989 EcoO109I rG'GnC_Cy Cuts at: 0 1892 3116 4913 6923 8972 10572 10709 12030 Size: 1892 1224 1797 2010 2049 1600 137 1321 Cuts at: 12030 12285 12703 12704 13053 13164 Size: 255 418 1 349 111 Fragments arranged by size: 2049 2010 1892 1797 1600 1321 1224 418 349 255 137 111 1 EcoRI G'AATT_C Cuts at: 0 862 3717 4957 5392 6815 7632 12790 13164 Size: 862 2855 1240 435 1423 817 5158 374 Fragments arranged by size: 5158 2855 1423 1240 862 817 435 374 EcoRV GAT'ATC Cuts at: 0 932 7725 8403 12144 13164 Size: 932 6793 678 3741 1020 Fragments arranged by size: 6793 3741 1020 932 678 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 211 355 2425 2706 3250 4143 5131 11218 Size: 211 144 2070 281 544 893 988 6087 Cuts at: 11218 11251 13164 Size: 33 1913 Fragments arranged by size: 6087 2070 1913 988 893 544 281 211 144 33 HaeII r_GCGC'y Cuts at: 0 6006 6054 8095 8649 12599 13164 Size: 6006 48 2041 554 3950 565 Fragments arranged by size: 6006 3950 2041 565 554 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 61 93 1033 1065 1354 1386 1466 1498 Size: 61 32 940 32 289 32 80 32 Cuts at: 1498 2656 2688 3214 3246 3259 3291 4003 4035 Size: 1158 32 526 32 13 32 712 32 Cuts at: 4035 4231 4263 4498 4515 4530 4547 5055 5087 Size: 196 32 235 17 15 17 508 32 Cuts at: 5087 5271 5303 6202 6234 6542 6574 7859 7891 Size: 184 32 899 32 308 32 1285 32 Cuts at: 7891 8476 8508 8537 8569 9190 9222 9792 9824 Size: 585 32 29 32 621 32 570 32 Cuts at: 9824 10207 10239 11784 11816 12360 12392 12595 12627 Size: 383 32 1545 32 544 32 203 32 Cuts at: 12627 12989 13021 13164 Size: 362 32 143 Fragments arranged by size: 1545 1285 1158 940 899 712 621 585 570 544 526 508 383 362 308 289 235 203 196 184 143 80 61 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 17 17 15 13 HincII GTy'rAC Cuts at: 0 3908 5418 9771 10416 11736 13164 Size: 3908 1510 4353 645 1320 1428 Fragments arranged by size: 4353 3908 1510 1428 1320 645 HindIII A'AGCT_T Cuts at: 0 3444 3803 4448 10234 13164 Size: 3444 359 645 5786 2930 Fragments arranged by size: 5786 3444 2930 645 359 HpaI GTT'AAC Cuts at: 0 5418 13164 Size: 5418 7746 KasI G'GCGC_C Cuts at: 0 6002 12595 13164 Size: 6002 6593 569 Fragments arranged by size: 6593 6002 569 KpnI G_GTAC'C Cuts at: 0 2948 11657 12411 13164 Size: 2948 8709 754 753 Fragments arranged by size: 8709 2948 754 753 MfeI C'AATT_G Cuts at: 0 2499 7592 7993 10386 13164 Size: 2499 5093 401 2393 2778 Fragments arranged by size: 5093 2778 2499 2393 401 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 293 1102 2463 2801 3115 3224 5304 5320 Size: 293 809 1361 338 314 109 2080 16 Cuts at: 5320 5549 7001 7845 10865 12029 13164 Size: 229 1452 844 3020 1164 1135 Fragments arranged by size: 3020 2080 1452 1361 1164 1135 844 809 338 314 293 229 109 16 MscI TGG'CCA Cuts at: 0 679 2317 6419 8313 13164 Size: 679 1638 4102 1894 4851 Fragments arranged by size: 4851 4102 1894 1638 679 MslI CAynn'nnrTG Cuts at: 0 2434 2657 2926 4622 5896 6850 6868 7483 Size: 2434 223 269 1696 1274 954 18 615 Cuts at: 7483 7546 8416 8891 11251 12113 12802 13164 Size: 63 870 475 2360 862 689 362 Fragments arranged by size: 2434 2360 1696 1274 954 870 862 689 615 475 362 269 223 63 18 MspA1I CmG'CkG Cuts at: 0 810 840 1801 2769 5985 6743 7079 7752 Size: 810 30 961 968 3216 758 336 673 Cuts at: 7752 7923 9652 9783 10938 11589 11725 11920 12536 Size: 171 1729 131 1155 651 136 195 616 Cuts at: 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2079 1566 1557 960 202 30 PacI TTA_AT'TAA Cuts at: 0 11525 13164 Size: 11525 1639 PasI CC'CwG_GG Cuts at: 0 6290 6306 8077 10120 12708 13164 Size: 6290 16 1771 2043 2588 456 Fragments arranged by size: 6290 2588 2043 1771 456 16 PciI A'CATG_T Cuts at: 0 2538 13164 Size: 2538 10626 PflMI CCAn_nnn'nTGG Cuts at: 0 2478 6486 8542 9344 9402 9610 12456 13164 Size: 2478 4008 2056 802 58 208 2846 708 Fragments arranged by size: 4008 2846 2478 2056 802 708 208 58 PfoI T'CCnGG_A Cuts at: 0 713 1239 1251 1311 2242 2513 2610 5236 Size: 713 526 12 60 931 271 97 2626 Cuts at: 5236 6068 6479 7697 10742 12158 13164 Size: 832 411 1218 3045 1416 1006 Fragments arranged by size: 3045 2626 1416 1218 1006 931 832 713 526 411 271 97 60 12 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 10598 10630 11298 11330 13164 Size: 10598 32 668 32 1834 Fragments arranged by size: 10598 1834 668 32 32 PpuMI rG'GwC_Cy Cuts at: 0 6923 8972 10572 10709 13053 13164 Size: 6923 2049 1600 137 2344 111 Fragments arranged by size: 6923 2344 2049 1600 137 111 PsiI TTA'TAA Cuts at: 0 3878 8270 12493 13164 Size: 3878 4392 4223 671 Fragments arranged by size: 4392 4223 3878 671 PspOMI G'GGCC_C Cuts at: 0 3116 4913 12703 13164 Size: 3116 1797 7790 461 Fragments arranged by size: 7790 3116 1797 461 PspXI vC'TCGA_Gb Cuts at: 0 1582 13164 Size: 1582 11582 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 8 40 1891 1923 8565 8597 11603 11635 Size: 8 32 1851 32 6642 32 3006 32 Cuts at: 11635 13164 Size: 1529 Fragments arranged by size: 6642 3006 1851 1529 32 32 32 32 8 PstI C_TGCA'G Cuts at: 0 1292 1443 1779 1806 4268 5517 5990 6504 Size: 1292 151 336 27 2462 1249 473 514 Cuts at: 6504 7800 7937 9369 9918 11067 11447 13164 Size: 1296 137 1432 549 1149 380 1717 Fragments arranged by size: 2462 1717 1432 1296 1292 1249 1149 549 514 473 380 336 151 137 27 PvuII CAG'CTG Cuts at: 0 810 840 1801 5985 7079 7752 7923 9652 Size: 810 30 961 4184 1094 673 171 1729 Cuts at: 9652 9783 10938 11589 11725 11920 13149 13164 Size: 131 1155 651 136 195 1229 15 Fragments arranged by size: 4184 1729 1229 1155 1094 961 810 673 651 195 171 136 131 30 15 SacI G_AGCT'C Cuts at: 0 10868 13164 Size: 10868 2296 SacII CC_GC'GG Cuts at: 0 6744 13164 Size: 6744 6420 SanDI GG'GwC_CC Cuts at: 0 6923 13164 Size: 6923 6241 SapI GCTCTTCn'nnn_ Cuts at: 0 8526 12766 13164 Size: 8526 4240 398 Fragments arranged by size: 8526 4240 398 ScaI AGT'ACT Cuts at: 0 3294 3983 13164 Size: 3294 689 9181 Fragments arranged by size: 9181 3294 689 SexAI A'CCwGG_T Cuts at: 0 11310 13164 Size: 11310 1854 SfcI C'TryA_G Cuts at: 0 1288 1439 1682 1775 1802 2566 3313 3722 Size: 1288 151 243 93 27 764 747 409 Cuts at: 3722 4264 4502 5513 5796 5986 6500 7316 7796 Size: 542 238 1011 283 190 514 816 480 Cuts at: 7796 7933 9292 9365 9914 11063 11443 13164 Size: 137 1359 73 549 1149 380 1721 Fragments arranged by size: 1721 1359 1288 1149 1011 816 764 747 549 542 514 480 409 380 283 243 238 190 151 137 93 73 27 SfoI GGC'GCC Cuts at: 0 6004 12597 13164 Size: 6004 6593 567 Fragments arranged by size: 6593 6004 567 SmlI C'TyrA_G Cuts at: 0 868 1582 1993 2932 3075 5072 5629 9172 Size: 868 714 411 939 143 1997 557 3543 Cuts at: 9172 11839 12025 12665 13164 Size: 2667 186 640 499 Fragments arranged by size: 3543 2667 1997 939 868 714 640 557 499 411 186 143 SnaBI TAC'GTA Cuts at: 0 10505 13164 Size: 10505 2659 SpeI A'CTAG_T Cuts at: 0 3190 13164 Size: 3190 9974 SphI G_CATG'C Cuts at: 0 10041 13164 Size: 10041 3123 SspI AAT'ATT Cuts at: 0 441 1066 9048 13164 Size: 441 625 7982 4116 Fragments arranged by size: 7982 4116 625 441 StuI AGG'CCT Cuts at: 0 11 1088 2970 10830 13164 Size: 11 1077 1882 7860 2334 Fragments arranged by size: 7860 2334 1882 1077 11 StyI C'CwwG_G Cuts at: 0 229 674 920 2051 3392 3917 4036 4917 Size: 229 445 246 1131 1341 525 119 881 Cuts at: 4917 5585 5784 6342 6684 7116 7154 8051 8308 Size: 668 199 558 342 432 38 897 257 Cuts at: 8308 8381 8444 10454 11252 12044 12122 12450 12552 Size: 73 63 2010 798 792 78 328 102 Cuts at: 12552 12945 13031 13164 Size: 393 86 133 Fragments arranged by size: 2010 1341 1131 897 881 798 792 668 558 525 445 432 393 342 328 257 246 229 199 133 119 102 86 78 73 63 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 222 1645 2080 2245 3153 3194 4324 5156 Size: 222 1423 435 165 908 41 1130 832 Cuts at: 5156 7129 8739 8905 9304 10899 13164 Size: 1973 1610 166 399 1595 2265 Fragments arranged by size: 2265 1973 1610 1595 1423 1130 908 832 435 399 222 166 165 41 TatI w'GTAC_w Cuts at: 0 564 3292 3981 4658 6842 8581 11628 12398 Size: 564 2728 689 677 2184 1739 3047 770 Cuts at: 12398 13164 Size: 766 Fragments arranged by size: 3047 2728 2184 1739 770 766 689 677 564 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1102 1134 2322 2354 3128 3160 5822 5854 Size: 1102 32 1188 32 774 32 2662 32 Cuts at: 5854 6050 6082 7830 7862 7887 7919 11558 11590 Size: 196 32 1748 32 25 32 3639 32 Cuts at: 11590 12860 12892 13164 Size: 1270 32 272 Fragments arranged by size: 3639 2662 1748 1270 1188 1102 774 272 196 32 32 32 32 32 32 32 32 32 25 Tth111I GACn'n_nGTC Cuts at: 0 6445 8965 13164 Size: 6445 2520 4199 Fragments arranged by size: 6445 4199 2520 XbaI T'CTAG_A Cuts at: 0 12087 13164 Size: 12087 1077 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2479 3157 3924 8394 10641 11259 13164 Size: 2479 678 767 4470 2247 618 1905 Fragments arranged by size: 4470 2479 2247 1905 767 678 618 XhoI C'TCGA_G Cuts at: 0 1582 5072 13164 Size: 1582 3490 8092 Fragments arranged by size: 8092 3490 1582 XmnI GAAnn'nnTTC Cuts at: 0 1172 1629 2408 3938 6300 7072 8843 10738 Size: 1172 457 779 1530 2362 772 1771 1895 Cuts at: 10738 10929 11231 13164 Size: 191 302 1933 Fragments arranged by size: 2362 1933 1895 1771 1530 1172 779 772 457 302 191 ZraI GAC'GTC Cuts at: 0 9768 13164 Size: 9768 3396 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PacI PasI PciI PflMI PfoI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuII SacI SacII SanDI SapI ScaI SexAI SfcI SfoI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI Enzymes that do not cut: AgeI AscI AsiSI BaeI BmtI BsiWI BssHII BstBI DrdI EagI FseI FspI FspAI MluI NheI NotI NruI PmeI PmlI PshAI PvuI RsrII SalI SbfI SfiI SgfI SgrAI SmaI SrfI SwaI XmaI