(Circular) (Six-base) MAPSORT of: pFN21AE2399.seq Check: 9345 from: 1 to: 11560 pFN21AE2399 11560 bp With 182 enzymes: SgfI * February 10, 2017 15:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4077 6892 4077 Size: 2815 8745 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9370 278 Size: 53 83 186 936 7834 2468 Fragments arranged by size: 7834 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3620 6285 7751 8903 1937 Size: 1683 2665 1466 1152 4594 Fragments arranged by size: 4594 2665 1683 1466 1152 Acc65I G'GTAC_C Cuts at: 3252 3291 8881 3252 Size: 39 5590 5931 Fragments arranged by size: 5931 5590 39 AclI AA'CG_TT Cuts at: 1563 9687 10060 1563 Size: 8124 373 3063 Fragments arranged by size: 8124 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2324 3884 7380 8274 8316 8410 9623 Size: 464 228 1560 3496 894 42 94 1213 Cuts at: 9623 11007 11275 1632 Size: 1384 268 1917 Fragments arranged by size: 3496 1917 1560 1384 1213 894 464 268 228 94 42 AfeI AGC'GCT Cuts at: 2553 2813 3791 7943 2553 Size: 260 978 4152 6170 Fragments arranged by size: 6170 4152 978 260 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11363 AflIII A'CryG_T Cuts at: 1203 7743 10459 11436 1203 Size: 6540 2716 977 1327 Fragments arranged by size: 6540 2716 1327 977 AgeI A'CCGG_T Cuts at: 10391 10391 Size: 11560 AhdI GACnn_n'nnGTC Cuts at: 7747 10289 7747 Size: 2542 9018 AleI CACnn'nnGTG Cuts at: 1166 8456 1166 Size: 7290 4270 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4190 4731 6273 8264 8474 1184 Size: 3006 541 1542 1991 210 4270 Fragments arranged by size: 4270 3006 1991 1542 541 210 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4771 4803 4771 Size: 32 11528 AlwNI CAG_nnn'CTG Cuts at: 2209 5366 6866 8353 9303 10875 2209 Size: 3157 1500 1487 950 1572 2894 Fragments arranged by size: 3157 2894 1572 1500 1487 950 ApaI G_GGCC'C Cuts at: 2186 2286 2330 2598 2964 2990 3064 5436 5759 Size: 100 44 268 366 26 74 2372 323 Cuts at: 5759 5917 6566 7695 8327 2186 Size: 158 649 1129 632 5419 Fragments arranged by size: 5419 2372 1129 649 632 366 323 268 158 100 74 44 26 ApaLI G'TGCA_C Cuts at: 9617 10773 9617 Size: 1156 10404 ApoI r'AATT_y Cuts at: 1493 5611 8869 9095 9247 11356 1493 Size: 4118 3258 226 152 2109 1697 Fragments arranged by size: 4118 3258 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4806 10114 160 Size: 872 3774 5308 1606 Fragments arranged by size: 5308 3774 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11560 AvaI C'yCGr_G Cuts at: 1870 1943 2704 2919 2939 3052 3095 5950 8206 Size: 73 761 215 20 113 43 2855 2256 Cuts at: 8206 8234 8885 1870 Size: 28 651 4545 Fragments arranged by size: 4545 2855 2256 761 651 215 113 73 43 28 20 AvrII C'CTAG_G Cuts at: 2330 2330 Size: 11560 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3236 3269 3485 3518 3236 Size: 33 216 33 11278 Fragments arranged by size: 11278 216 33 33 BamHI G'GATC_C Cuts at: 5562 5695 7240 8890 9257 5562 Size: 133 1545 1650 367 7865 Fragments arranged by size: 7865 1650 1545 367 133 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2404 2975 3252 3291 4511 Size: 325 217 627 617 571 277 39 1220 Cuts at: 4511 6044 6410 6488 8083 8881 10336 618 Size: 1533 366 78 1595 798 1455 1842 Fragments arranged by size: 1842 1595 1533 1455 1220 798 627 617 571 366 325 277 217 78 39 BanII G_rGCy'C Cuts at: 1391 2186 2286 2330 2477 2586 2598 2702 2726 Size: 795 100 44 147 109 12 104 24 Cuts at: 2726 2964 2990 3064 3095 3973 4364 5436 5759 Size: 238 26 74 31 878 391 1072 323 Cuts at: 5759 5917 6551 6566 7149 7695 8327 8879 1391 Size: 158 634 15 583 546 632 552 4072 Fragments arranged by size: 4072 1072 878 795 634 632 583 552 546 391 323 238 158 147 109 104 100 74 44 31 26 24 15 12 BbeI G_GCGC'C Cuts at: 2408 2979 2408 Size: 571 10989 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2500 2784 5343 928 Size: 971 601 284 2559 7145 Fragments arranged by size: 7145 2559 971 601 284 BbvCI CC'TCA_GC Cuts at: 3138 3180 3438 3699 3744 3844 6204 6273 6471 Size: 42 258 261 45 100 2360 69 198 Cuts at: 6471 8369 3138 Size: 1898 6329 Fragments arranged by size: 6329 2360 1898 261 258 198 100 69 45 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3286 3320 5914 5948 9751 9785 1903 Size: 34 1349 34 2594 34 3803 34 3678 Fragments arranged by size: 3803 3678 2594 1349 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3750 6769 9452 10662 3750 Size: 3019 2683 1210 4648 Fragments arranged by size: 4648 3019 2683 1210 BclI T'GATC_A Cuts at: 1548 4824 7419 1548 Size: 3276 2595 5689 Fragments arranged by size: 5689 3276 2595 BfrBI ATG'CAT Cuts at: 4216 4528 4708 4216 Size: 312 180 11068 Fragments arranged by size: 11068 312 180 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2498 4183 5763 Size: 107 122 71 631 605 826 1685 1580 Cuts at: 5763 7609 8525 10171 136 Size: 1846 916 1646 1525 Fragments arranged by size: 1846 1685 1646 1580 1525 916 826 631 605 122 107 71 BglII A'GATC_T Cuts at: 7182 11555 7182 Size: 4373 7187 BlpI GC'TnA_GC Cuts at: 2696 2805 4309 6480 8977 2696 Size: 109 1504 2171 2497 5279 Fragments arranged by size: 5279 2497 2171 1504 109 Bme1580I G_kGCm'C Cuts at: 1790 2186 2286 2330 2598 2964 2990 3064 5255 Size: 396 100 44 268 366 26 74 2191 Cuts at: 5255 5436 5759 5917 6566 6690 7695 8327 9621 Size: 181 323 158 649 124 1005 632 1294 Cuts at: 9621 10777 1790 Size: 1156 2573 Fragments arranged by size: 2573 2191 1294 1156 1005 649 632 396 366 323 268 181 158 124 100 74 44 26 BmgBI CAC'GTC Cuts at: 1318 2904 1318 Size: 1586 9974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3745 4185 4279 Size: 416 53 83 399 24 2328 440 94 Cuts at: 4279 4470 5440 5637 5919 6198 6854 8284 9291 Size: 191 970 197 282 279 656 1430 1007 Cuts at: 9291 10249 442 Size: 958 1753 Fragments arranged by size: 2328 1753 1430 1007 970 958 656 440 416 399 282 279 197 191 94 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 11560 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5736 8764 5736 Size: 3028 8532 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2600 2752 3521 3847 3898 4002 4512 4551 Size: 1361 152 769 326 51 104 510 39 Cuts at: 4551 4917 5481 5706 7695 8456 10220 1239 Size: 366 564 225 1989 761 1764 2579 Fragments arranged by size: 2579 1989 1764 1361 769 761 564 510 366 326 225 152 104 51 39 Bpu10I CC'TnA_GC Cuts at: 3138 3180 3438 3699 3744 3844 3893 4135 6204 Size: 42 258 261 45 100 49 242 2069 Cuts at: 6204 6273 6471 8369 8529 3138 Size: 69 198 1898 160 6169 Fragments arranged by size: 6169 2069 1898 261 258 242 198 160 100 69 49 45 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3801 7016 9685 10550 10848 3801 Size: 3215 2669 865 298 4513 Fragments arranged by size: 4513 3215 2669 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3169 3837 6381 7046 7889 10223 882 Size: 617 1670 668 2544 665 843 2334 2219 Fragments arranged by size: 2544 2334 2219 1670 843 668 665 617 BsaAI yAC'GTr Cuts at: 493 1204 9351 493 Size: 711 8147 2702 Fragments arranged by size: 8147 2702 711 BsaBI GATnn'nnATC Cuts at: 5419 7181 11554 5419 Size: 1762 4373 5425 Fragments arranged by size: 5425 4373 1762 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2405 2976 9367 9749 Size: 53 83 186 936 872 571 6391 382 Cuts at: 9749 10384 275 Size: 635 1451 Fragments arranged by size: 6391 1451 936 872 635 571 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4334 4677 7851 9993 10391 10665 10812 11223 4334 Size: 343 3174 2142 398 274 147 411 4671 Fragments arranged by size: 4671 3174 2142 411 398 343 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3519 3549 4378 4408 4549 Size: 30 165 30 2116 30 829 30 141 Cuts at: 4549 4564 4579 4594 4771 4801 8135 8165 8429 Size: 15 15 15 177 30 3334 30 264 Cuts at: 8429 8459 1178 Size: 30 4279 Fragments arranged by size: 4279 3334 2116 829 264 177 165 141 30 30 30 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2400 2755 2773 4041 5114 5490 6985 Size: 931 283 355 18 1268 1073 376 1495 Cuts at: 6985 7376 7424 8120 8639 1186 Size: 391 48 696 519 4107 Fragments arranged by size: 4107 1495 1268 1073 931 696 519 391 376 355 283 48 18 BseYI C'CCAG_C Cuts at: 2209 2440 2598 2865 2909 3380 3462 3561 3573 Size: 231 158 267 44 471 82 99 12 Cuts at: 3573 3612 3726 3765 3783 4299 5044 5382 6068 Size: 39 114 39 18 516 745 338 686 Cuts at: 6068 6223 6228 6234 6261 6420 7837 10763 11259 Size: 155 5 6 27 159 1417 2926 496 Cuts at: 11259 2209 Size: 2510 Fragments arranged by size: 2926 2510 1417 745 686 516 496 471 338 267 231 159 158 155 114 99 82 44 39 39 27 18 12 6 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4500 6384 6587 6809 6914 7514 7526 Size: 573 2764 1884 203 222 105 600 12 Cuts at: 7526 8039 8585 1163 Size: 513 546 4138 Fragments arranged by size: 4138 2764 1884 600 573 546 513 222 203 105 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2139 2427 2470 2502 2850 3048 Size: 275 186 138 288 43 32 348 198 Cuts at: 3048 9010 9771 10799 1540 Size: 5962 761 1028 2301 Fragments arranged by size: 5962 2301 1028 761 348 288 275 198 186 138 43 32 BsiHKAI G_wGCw'C Cuts at: 2702 3973 3992 5909 6545 7058 7149 9621 9706 Size: 1271 19 1917 636 513 91 2472 85 Cuts at: 9706 10777 2702 Size: 1071 3485 Fragments arranged by size: 3485 2472 1917 1271 1071 636 513 91 85 19 BsmI GAATG_Cn' Cuts at: 5321 9071 9164 5321 Size: 3750 93 7717 Fragments arranged by size: 7717 3750 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 2286 2330 2477 2586 2598 2702 Size: 399 396 100 44 147 109 12 104 Cuts at: 2702 2726 2964 2990 3064 3095 3973 3992 4364 Size: 24 238 26 74 31 878 19 372 Cuts at: 4364 5255 5436 5759 5909 5917 6545 6551 6566 Size: 891 181 323 150 8 628 6 15 Cuts at: 6566 6690 7058 7149 7695 8327 8879 9621 9706 Size: 124 368 91 546 632 552 742 85 Cuts at: 9706 10777 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 891 878 742 632 628 552 546 399 396 372 368 323 238 181 150 147 124 109 104 100 91 85 74 44 31 26 24 19 15 12 8 6 BspEI T'CCGG_A Cuts at: 4334 11223 4334 Size: 6889 4671 BspHI T'CATG_A Cuts at: 7107 9449 11179 7107 Size: 2342 1730 7488 Fragments arranged by size: 7488 2342 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2005 4077 6842 6874 6892 8915 844 Size: 1161 2072 2765 32 18 2023 3489 Fragments arranged by size: 3489 2765 2072 2023 1161 32 18 BsrBI CCG'CTC Cuts at: 1394 1823 2562 3124 9447 1394 Size: 429 739 562 6323 3507 Fragments arranged by size: 6323 3507 739 562 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2248 4239 5528 6307 7999 8556 10055 Size: 1183 1000 1991 1289 779 1692 557 1499 Cuts at: 10055 10229 65 Size: 174 1396 Fragments arranged by size: 1991 1692 1499 1396 1289 1183 1000 779 557 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2267 2744 2849 2978 4599 5795 10204 Size: 110 457 477 105 129 1621 1196 4409 Cuts at: 10204 10391 1700 Size: 187 2869 Fragments arranged by size: 4409 2869 1621 1196 477 457 187 129 110 105 BsrGI T'GTAC_A Cuts at: 96 6168 96 Size: 6072 5488 BssHII G'CGCG_C Cuts at: 1925 8712 1925 Size: 6787 4773 BssSI C'ACGA_G Cuts at: 6634 8550 9620 10632 6634 Size: 1916 1070 1012 7562 Fragments arranged by size: 7562 1916 1070 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6217 6430 8552 794 Size: 5423 213 2122 3802 Fragments arranged by size: 5423 3802 2122 213 BstBI TT'CG_AA Cuts at: 7969 10364 7969 Size: 2395 9165 BstEII G'GTnAC_C Cuts at: 1184 5774 7214 1184 Size: 4590 1440 5530 Fragments arranged by size: 5530 4590 1440 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2522 3154 2041 Size: 481 632 10447 Fragments arranged by size: 10447 632 481 BstYI r'GATC_y Cuts at: 1972 5391 5420 5520 5562 5695 7182 7240 7863 Size: 3419 29 100 42 133 1487 58 623 Cuts at: 7863 8890 9257 9642 9659 11111 11197 11209 11555 Size: 1027 367 385 17 1452 86 12 346 Cuts at: 11555 1972 Size: 1977 Fragments arranged by size: 3419 1977 1487 1452 1027 623 385 367 346 133 100 86 58 42 29 17 12 BstZ17I GTA'TAC Cuts at: 6286 6286 Size: 11560 Bsu36I CC'TnA_GG Cuts at: 4635 4833 5746 5892 6753 4635 Size: 198 913 146 861 9442 Fragments arranged by size: 9442 913 861 198 146 BtgI C'CryG_G Cuts at: 513 1065 2004 2190 2421 2496 2617 2823 2844 Size: 552 939 186 231 75 121 206 21 Cuts at: 2844 3327 3954 4380 4506 4590 4851 4863 5109 Size: 483 627 426 126 84 261 12 246 Cuts at: 5109 5463 6646 7278 513 Size: 354 1183 632 4795 Fragments arranged by size: 4795 1183 939 632 627 552 483 426 354 261 246 231 206 186 126 121 84 75 21 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2406 5834 487 Size: 683 56 1180 3428 6213 Fragments arranged by size: 6213 3428 1180 683 56 BtsI GCAGTG_nn' Cuts at: 3014 4164 5269 6315 8568 9076 9870 9890 3014 Size: 1150 1105 1046 2253 508 794 20 4684 Fragments arranged by size: 4684 2253 1150 1105 1046 794 508 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2230 2265 8667 8702 529 Size: 35 1666 35 6402 35 3387 Fragments arranged by size: 6402 3387 1666 35 35 35 DraI TTT'AAA Cuts at: 7197 8864 7197 Size: 1667 9893 DraIII CAC_nnn'GTG Cuts at: 4193 4424 6495 4193 Size: 231 2071 9258 Fragments arranged by size: 9258 2071 231 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5446 10567 809 Size: 507 4130 5121 1802 Fragments arranged by size: 5121 4130 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2136 2424 Size: 54 1425 50 275 18 177 129 288 Cuts at: 2424 2467 2499 2847 3045 4184 4195 4620 4866 Size: 43 32 348 198 1139 11 425 246 Cuts at: 4866 6116 6382 6782 8412 8517 8727 8851 9007 Size: 1250 266 400 1630 105 210 124 156 Cuts at: 9007 9896 11525 8 Size: 889 1629 43 Fragments arranged by size: 1630 1629 1425 1250 1139 889 425 400 348 288 275 266 246 210 198 177 156 129 124 105 54 50 43 43 32 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2136 2424 2467 2499 2847 3045 9007 Size: 275 324 288 43 32 348 198 5962 Cuts at: 9007 1537 Size: 4090 Fragments arranged by size: 5962 4090 348 324 288 275 198 43 32 EarI CTCTTCn'nnn_ Cuts at: 1353 7320 7332 9490 1353 Size: 5967 12 2158 3423 Fragments arranged by size: 5967 3423 2158 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2277 2541 2767 3008 7552 10147 Size: 1064 138 688 264 226 241 4544 2595 Cuts at: 10147 10521 10667 387 Size: 374 146 1280 Fragments arranged by size: 4544 2595 1280 1064 688 374 264 241 226 146 138 EcoICRI GAG'CTC Cuts at: 2700 3971 7147 2700 Size: 1271 3176 7113 Fragments arranged by size: 7113 3176 1271 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2324 2600 2752 3521 3847 3884 Size: 393 464 228 276 152 769 326 37 Cuts at: 3884 3898 4002 4512 4551 4917 5481 5706 7380 Size: 14 104 510 39 366 564 225 1674 Cuts at: 7380 7695 8274 8316 8410 8456 9623 10220 11007 Size: 315 579 42 94 46 1167 597 787 Cuts at: 11007 11275 1239 Size: 268 1524 Fragments arranged by size: 1674 1524 1167 787 769 597 579 564 510 464 393 366 326 315 276 268 228 225 152 104 94 46 42 39 37 14 EcoNI CCTnn'n_nnAGG Cuts at: 3228 4335 5805 5883 8528 3228 Size: 1107 1470 78 2645 6260 Fragments arranged by size: 6260 2645 1470 1107 78 EcoO109I rG'GnC_Cy Cuts at: 2182 2183 2327 2575 2594 2595 2961 2986 3060 Size: 1 144 248 19 1 366 25 74 Cuts at: 3060 3221 3273 3377 4085 4838 5432 5433 5755 Size: 161 52 104 708 753 594 1 322 Cuts at: 5755 5756 5852 5888 5913 5914 6562 6563 6702 Size: 1 96 36 25 1 648 1 139 Cuts at: 6702 6854 7313 7691 7692 8305 8324 2182 Size: 152 459 378 1 613 19 5418 Fragments arranged by size: 5418 753 708 648 613 594 459 378 366 322 248 161 152 144 139 104 96 74 52 36 25 25 19 19 1 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 5611 8869 9247 5611 Size: 3258 378 7924 Fragments arranged by size: 7924 3258 378 EcoRV GAT'ATC Cuts at: 6919 6919 Size: 11560 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 7322 7322 Size: 11560 FspI TGC'GCA Cuts at: 10066 10066 Size: 11560 HaeII r_GCGC'y Cuts at: 1678 2408 2555 2667 2775 2815 2979 3793 6340 Size: 730 147 112 108 40 164 814 2547 Cuts at: 6340 7945 10707 1678 Size: 1605 2762 2531 Fragments arranged by size: 2762 2547 2531 1605 814 730 164 147 112 108 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2987 3019 3163 3195 4165 Size: 32 135 32 1415 32 144 32 970 Cuts at: 4165 4197 4378 4410 4564 4596 4816 4848 5383 Size: 32 181 32 154 32 220 32 535 Cuts at: 5383 5415 5947 5979 6378 6380 6410 6412 7733 Size: 32 532 32 399 2 30 2 1321 Cuts at: 7733 7765 10201 10233 10275 10307 1373 Size: 32 2436 32 42 32 2626 Fragments arranged by size: 2626 2436 1415 1321 970 535 532 399 220 181 154 144 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3395 3621 4929 5206 5451 8904 Size: 988 281 1457 226 1308 277 245 3453 Cuts at: 8904 9150 669 Size: 246 3079 Fragments arranged by size: 3453 3079 1457 1308 988 281 277 246 245 226 HindIII A'AGCT_T Cuts at: 748 7436 7493 748 Size: 6688 57 4815 Fragments arranged by size: 6688 4815 57 HpaI GTT'AAC Cuts at: 4929 9150 4929 Size: 4221 7339 KasI G'GCGC_C Cuts at: 2404 2975 2404 Size: 571 10989 KpnI G_GTAC'C Cuts at: 3256 3295 8885 3256 Size: 39 5590 5931 Fragments arranged by size: 5931 5590 39 MfeI C'AATT_G Cuts at: 9159 9159 Size: 11560 MluI A'CGCG_T Cuts at: 11436 11436 Size: 11560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4182 5964 7189 8196 8760 10674 10858 1305 Size: 2877 1782 1225 1007 564 1914 184 2007 Fragments arranged by size: 2877 2007 1914 1782 1225 1007 564 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4197 4622 4868 8414 8519 Size: 54 1425 343 2365 425 246 3546 105 Cuts at: 8519 8729 8853 10 Size: 210 124 2717 Fragments arranged by size: 3546 2717 2365 1425 425 343 246 210 124 105 54 MslI CAynn'nnrTG Cuts at: 518 1166 4355 4943 5114 8456 8561 9518 9877 Size: 648 3189 588 171 3342 105 957 359 Cuts at: 9877 10036 518 Size: 159 2042 Fragments arranged by size: 3342 3189 2042 957 648 588 359 171 159 105 MspA1I CmG'CkG Cuts at: 1606 2098 2135 2209 2274 2423 2498 2846 3035 Size: 492 37 74 65 149 75 348 189 Cuts at: 3035 3105 3289 3384 5363 6326 6470 8294 8976 Size: 70 184 95 1979 963 144 1824 682 Cuts at: 8976 9653 10801 11046 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1979 1824 1148 963 682 677 492 348 245 189 184 149 144 95 75 74 70 65 37 NaeI GCC'GGC Cuts at: 2269 2746 2851 2980 5797 2269 Size: 477 105 129 2817 8032 Fragments arranged by size: 8032 2817 477 129 105 NarI GG'CG_CC Cuts at: 2405 2976 2405 Size: 571 10989 NcoI C'CATG_G Cuts at: 513 1065 2004 2823 3327 3954 4380 4506 4590 Size: 552 939 819 504 627 426 126 84 Cuts at: 4590 4851 4863 5109 5463 6646 7278 513 Size: 261 12 246 354 1183 632 4795 Fragments arranged by size: 4795 1183 939 819 632 627 552 504 426 354 261 246 126 84 12 NdeI CA'TA_TG Cuts at: 387 3187 6133 6667 387 Size: 2800 2946 534 5280 Fragments arranged by size: 5280 2946 2800 534 NgoMIV G'CCGG_C Cuts at: 2267 2744 2849 2978 5795 2267 Size: 477 105 129 2817 8032 Fragments arranged by size: 8032 2817 477 129 105 NheI G'CTAG_C Cuts at: 1052 1052 Size: 11560 NotI GC'GGCC_GC Cuts at: 2424 2499 3045 9007 2424 Size: 75 546 5962 4977 Fragments arranged by size: 5962 4977 546 75 NsiI A_TGCA'T Cuts at: 4218 4530 4710 4218 Size: 312 180 11068 Fragments arranged by size: 11068 312 180 NspI r_CATG'y Cuts at: 1132 1229 4712 5677 6433 6739 7649 7747 8659 Size: 97 3483 965 756 306 910 98 912 Cuts at: 8659 8918 10463 1132 Size: 259 1545 2229 Fragments arranged by size: 3483 2229 1545 965 912 910 756 306 259 98 97 PasI CC'CwG_GG Cuts at: 1348 3257 4399 5630 5801 6559 7133 8018 8320 Size: 1909 1142 1231 171 758 574 885 302 Cuts at: 8320 1348 Size: 4588 Fragments arranged by size: 4588 1909 1231 1142 885 758 574 302 171 PciI A'CATG_T Cuts at: 7743 10459 7743 Size: 2716 8844 PflMI CCAn_nnn'nTGG Cuts at: 1108 4193 4424 4553 5630 6125 7703 1108 Size: 3085 231 129 1077 495 1578 4965 Fragments arranged by size: 4965 3085 1578 1077 495 231 129 PfoI T'CCnGG_A Cuts at: 3979 5565 8172 8256 8385 3979 Size: 1586 2607 84 129 7154 Fragments arranged by size: 7154 2607 1586 129 84 PmeI GTTT'AAAC Cuts at: 8864 8864 Size: 11560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4771 4803 6785 6817 9629 9661 11142 11174 4771 Size: 32 1982 32 2812 32 1481 32 5157 Fragments arranged by size: 5157 2812 1982 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3221 3273 4838 5852 6854 7313 8305 3221 Size: 52 1565 1014 1002 459 992 6476 Fragments arranged by size: 6476 1565 1014 1002 992 459 52 PshAI GACnn'nnGTC Cuts at: 1613 3226 5201 1613 Size: 1613 1975 7972 Fragments arranged by size: 7972 1975 1613 PsiI TTA'TAA Cuts at: 9130 9130 Size: 11560 PspOMI G'GGCC_C Cuts at: 2182 2282 2326 2594 2960 2986 3060 5432 5755 Size: 100 44 268 366 26 74 2372 323 Cuts at: 5755 5913 6562 7691 8323 2182 Size: 158 649 1129 632 5419 Fragments arranged by size: 5419 2372 1129 649 632 366 323 268 158 100 74 44 26 PstI C_TGCA'G Cuts at: 830 2226 2559 4159 4882 8912 830 Size: 1396 333 1600 723 4030 3478 Fragments arranged by size: 4030 3478 1600 1396 723 333 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11560 PvuII CAG'CTG Cuts at: 3035 5363 6326 6470 8294 3035 Size: 2328 963 144 1824 6301 Fragments arranged by size: 6301 2328 1824 963 144 SacI G_AGCT'C Cuts at: 2702 3973 7149 2702 Size: 1271 3176 7113 Fragments arranged by size: 7113 3176 1271 SacII CC_GC'GG Cuts at: 2424 2499 2847 2424 Size: 75 348 11137 Fragments arranged by size: 11137 348 75 SalI G'TCGA_C Cuts at: 1936 3619 8902 1936 Size: 1683 5283 4594 Fragments arranged by size: 5283 4594 1683 SanDI GG'GwC_CC Cuts at: 3273 4838 3273 Size: 1565 9995 SbfI CC_TGCA'GG Cuts at: 8912 8912 Size: 11560 ScaI AGT'ACT Cuts at: 9808 11520 9808 Size: 1712 9848 SfcI C'TryA_G Cuts at: 826 1045 2222 2555 2950 4155 4878 5782 8908 Size: 219 1177 333 395 1205 723 904 3126 Cuts at: 8908 10043 10724 10915 826 Size: 1135 681 191 1471 Fragments arranged by size: 3126 1471 1205 1177 1135 904 723 681 395 333 219 191 SfoI GGC'GCC Cuts at: 2406 2977 2406 Size: 571 10989 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11560 SmaI CCC'GGG Cuts at: 1872 2921 2941 3097 8887 1872 Size: 1049 20 156 5790 4545 Fragments arranged by size: 5790 4545 1049 156 20 SmlI C'TyrA_G Cuts at: 820 1017 1943 3816 7031 9664 10565 10827 820 Size: 197 926 1873 3215 2633 901 262 1553 Fragments arranged by size: 3215 2633 1873 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 9351 493 Size: 8858 2702 SpeI A'CTAG_T Cuts at: 152 8681 152 Size: 8529 3031 SphI G_CATG'C Cuts at: 1132 6739 8918 1132 Size: 5607 2179 3774 Fragments arranged by size: 5607 3774 2179 SspI AAT'ATT Cuts at: 5 52 7160 9484 5 Size: 47 7108 2324 2081 Fragments arranged by size: 7108 2324 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 11560 StyI C'CwwG_G Cuts at: 513 1065 2004 2330 2823 3327 3954 4012 4380 Size: 552 939 326 493 504 627 58 368 Cuts at: 4380 4506 4590 4851 4863 4912 5109 5463 6040 Size: 126 84 261 12 49 197 354 577 Cuts at: 6040 6646 7278 8119 8184 8724 8730 8999 513 Size: 606 632 841 65 540 6 269 3074 Fragments arranged by size: 3074 939 841 632 627 606 577 552 540 504 493 368 354 326 269 261 197 126 84 65 58 49 12 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4204 4499 4844 4984 5036 5062 5762 Size: 1255 2624 295 345 140 52 26 700 Cuts at: 5762 6053 7202 7746 7787 8031 8082 8490 9587 Size: 291 1149 544 41 244 51 408 1097 Cuts at: 9587 9604 9757 9942 10387 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2624 1498 1255 1149 1097 700 544 445 408 345 295 291 244 185 153 140 52 51 41 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2637 4287 6168 Size: 274 80 33 51 1443 660 1650 1881 Cuts at: 6168 8836 9806 11518 96 Size: 2668 970 1712 138 Fragments arranged by size: 2668 1881 1712 1650 1443 970 660 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3519 3551 4549 4581 8135 8167 11520 11552 3519 Size: 32 998 32 3554 32 3353 32 3527 Fragments arranged by size: 3554 3527 3353 998 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6392 1316 Size: 5076 6484 XbaI T'CTAG_A Cuts at: 8896 8896 Size: 11560 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2519 4483 5245 5461 5767 6665 2519 Size: 1964 762 216 306 898 7414 Fragments arranged by size: 7414 1964 898 762 306 216 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11560 XmaI C'CCGG_G Cuts at: 1870 2919 2939 3095 8885 1870 Size: 1049 20 156 5790 4545 Fragments arranged by size: 5790 4545 1049 156 20 XmnI GAAnn'nnTTC Cuts at: 7305 9689 7305 Size: 2384 9176 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9368 276 Size: 53 83 186 936 7834 2468 Fragments arranged by size: 7834 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BsiWI BsmBI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI