################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BciVI BfaI BfrI BglI BlnI BpuAI BsaAI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MluI MroI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 367 GTAGAC GT^[AC][GT]AC ApaI 1074 GGGCCC GGGCC^C AspI 1106 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 752 GGATCC G^GATCC BanI 8 GGCACC G^G[CT][GA]CC BcgI 977 GCACGAGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 381 CCGGG CC^[GC]GG BglII 413 AGATCT A^GATCT BlpI 620 GCTCAGC GC^T[ACGT]AGC Bpu1102I 620 GCTCAGC GC^T[ACGT]AGC BsaBI 753 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 772 CTGCAC GTGCAG(16/14) BsmAI 651 GAGAC GTCTC(1/5) BsrBI 807 CCGCTC CCGCTC(-3/-3) BsrI 275 ACTGG ACTGG(1/-1) BssSI 978 CACGAG CACGAG(-5/-1) BstAPI 9 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 620 GCTCAGC GC^T[ACGT]AGC CfoI 1019 GCGC GCG^C DraII 998 GGGACCC [GA]G^G[ACGT]CC[CT] EarI 310 CTCTTC CTCTTC(1/4) EcoO109I 998 GGGACCC [GA]G^G[ACGT]CC[CT] HaeII 1018 GGCGCT [GA]GCGC^[CT] HgaI 983 GCGTC GACGC(5/10) HhaI 1019 GCGC GCG^C HinP1I 1019 GCGC G^CGC HinfI 3 GAATC G^A[ACGT]TC Ksp632I 310 CTCTTC CTCTTC(1/4) MaeIII 1112 GTCAC ^GT[ACGT]AC MamI 753 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 420 CAATTG C^AATTG MseI 1033 TTAA T^TAA MspA1I 930 CCGCTG C[AC]G^C[GT]G MunI 420 CAATTG C^AATTG NaeI 299 GCCGGC GCC^GGC NciI 381 CCGGG CC^[GC]GG NcoI 1054 CCATGG C^CATGG NgoMIV 299 GCCGGC G^CCGGC PflFI 1106 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 998 GGGACCC [GA]G^G[AT]CC[CT] PstI 16 CTGCAG CTGCA^G SacI 988 GAGCTC GAGCT^C SapI 309 GCTCTTC GCTCTTC(1/4) SfcI 16 CTGCAG C^T[GA][CT]AG SgrAI 92 CGCCGGTG C[GA]^CCGG[CT]G SmlI 166 CTCAAG C^T[CT][GA]AG TfiI 3 GAATC G^A[AT]TC Tru9I 1033 TTAA T^TAA Tsp45I 1112 GTCAC ^GT[GC]AC TspRI 462 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 1106 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 804 GTAC GT^AC 879 GTAC AlwNI 209 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG 540 CAGCATCTG AspHI 307 GTGCTC G[AT]GC[AT]^C 988 GAGCTC BalI 707 TGGCCA TGG^CCA 1037 TGGCCA BclI 227 TGATCA T^GATCA 923 TGATCA BsaWI 557 TCCGGT [AT]^CCGG[AT] 1045 TCCGGT BsiHKAI 307 GTGCTC G[AT]GC[AT]^C 988 GAGCTC BsrDI 75 CATTGC GCAATG(2/0) 131 GCAATG BstYI 413 AGATCT [GA]^GATC[CT] 752 GGATCC DdeI 621 CTCAG C^T[ACGT]AG 838 CTCAG DsaI 872 CCGTGG C^C[GA][CT]GG 1054 CCATGG EaeI 707 TGGCCA [CT]^GGCC[GA] 1037 TGGCCA EcoT14I 80 CCAAGG C^C[AT][AT]GG 1054 CCATGG FbaI 227 TGATCA T^GATCA 923 TGATCA MflI 413 AGATCT [GA]^GATC[CT] 752 GGATCC MluNI 707 TGGCCA TGG^CCA 1037 TGGCCA MscI 707 TGGCCA TGG^CCA 1037 TGGCCA NspI 666 GCATGC [GA]CATG^[CT] 860 GCATGC RsaI 804 GTAC GT^AC 879 GTAC SphI 666 GCATGC GCATG^C 860 GCATGC StyI 80 CCAAGG C^C[AT][AT]GG 1054 CCATGG TaqI 986 TCGA T^CGA 1157 TCGA TthHB8I 986 TCGA T^CGA 1157 TCGA XhoII 413 AGATCT [GA]^GATC[CT] 752 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboI MboII MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp509I