(Linear) (Six-base) MAPSORT of: KIEE3446.seq Check: 9528 from: 1 to: 1161 KIEE3446.orf With 182 enzymes: SgfI * January 29, 2014 11:45 .. AccI GT'mk_AC Cuts at: 0 368 1161 Size: 368 793 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 522 1161 Size: 508 14 639 Fragments arranged by size: 639 508 14 AleI CACnn'nnGTG Cuts at: 0 358 1161 Size: 358 803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 543 1161 Size: 543 618 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 400 432 808 840 1161 Size: 400 32 376 32 321 Fragments arranged by size: 400 376 321 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 214 545 1161 Size: 214 331 616 Fragments arranged by size: 616 331 214 ApaI G_GGCC'C Cuts at: 0 1078 1161 Size: 1078 83 BamHI G'GATC_C Cuts at: 0 752 1161 Size: 752 409 BanI G'GyrC_C Cuts at: 0 8 1161 Size: 8 1153 BanII G_rGCy'C Cuts at: 0 992 1063 1078 1161 Size: 992 71 15 83 Fragments arranged by size: 992 83 71 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 966 1000 1161 Size: 966 34 161 Fragments arranged by size: 966 161 34 BclI T'GATC_A Cuts at: 0 227 923 1161 Size: 227 696 238 Fragments arranged by size: 696 238 227 BglII A'GATC_T Cuts at: 0 413 1161 Size: 413 748 BlpI GC'TnA_GC Cuts at: 0 621 1161 Size: 621 540 Bme1580I G_kGCm'C Cuts at: 0 787 1078 1161 Size: 787 291 83 Fragments arranged by size: 787 291 83 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 604 657 873 922 1007 1153 1161 Size: 127 477 53 216 49 85 146 8 Fragments arranged by size: 477 216 146 127 85 53 49 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 1161 Size: 151 1010 BsaBI GATnn'nnATC Cuts at: 0 757 1161 Size: 757 404 BsaWI w'CCGG_w Cuts at: 0 557 1045 1161 Size: 557 488 116 Fragments arranged by size: 557 488 116 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 400 430 871 898 901 928 1126 1156 Size: 400 30 441 27 3 27 198 30 Cuts at: 1156 1161 Size: 5 Fragments arranged by size: 441 400 198 30 30 27 27 5 3 BseYI C'CCAG_C Cuts at: 0 736 1161 Size: 736 425 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 757 1161 Size: 757 404 BsiHKAI G_wGCw'C Cuts at: 0 311 992 1161 Size: 311 681 169 Fragments arranged by size: 681 311 169 Bsp1286I G_dGCh'C Cuts at: 0 311 787 992 1063 1078 1161 Size: 311 476 205 71 15 83 Fragments arranged by size: 476 311 205 83 71 15 BsrBI CCG'CTC Cuts at: 0 809 1161 Size: 809 352 BsrDI GCAATG_nn' Cuts at: 0 74 138 1161 Size: 74 64 1023 Fragments arranged by size: 1023 74 64 BsrFI r'CCGG_y Cuts at: 0 93 299 662 1161 Size: 93 206 363 499 Fragments arranged by size: 499 363 206 93 BssSI C'ACGA_G Cuts at: 0 978 1161 Size: 978 183 BstAPI GCAn_nnn'nTGC Cuts at: 0 15 1161 Size: 15 1146 BstYI r'GATC_y Cuts at: 0 413 752 1161 Size: 413 339 409 Fragments arranged by size: 413 409 339 BtgI C'CryG_G Cuts at: 0 872 1054 1161 Size: 872 182 107 Fragments arranged by size: 872 182 107 EaeI y'GGCC_r Cuts at: 0 707 1037 1161 Size: 707 330 124 Fragments arranged by size: 707 330 124 EarI CTCTTCn'nnn_ Cuts at: 0 316 1161 Size: 316 845 EcoICRI GAG'CTC Cuts at: 0 990 1161 Size: 990 171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 508 522 604 657 873 922 1007 Size: 127 381 14 82 53 216 49 85 Cuts at: 1007 1153 1161 Size: 146 8 Fragments arranged by size: 381 216 146 127 85 82 53 49 14 8 EcoO109I rG'GnC_Cy Cuts at: 0 999 1161 Size: 999 162 HaeII r_GCGC'y Cuts at: 0 1022 1161 Size: 1022 139 MfeI C'AATT_G Cuts at: 0 420 1161 Size: 420 741 MscI TGG'CCA Cuts at: 0 709 1039 1161 Size: 709 330 122 Fragments arranged by size: 709 330 122 MslI CAynn'nnrTG Cuts at: 0 358 700 703 850 1161 Size: 358 342 3 147 311 Fragments arranged by size: 358 342 311 147 3 MspA1I CmG'CkG Cuts at: 0 932 1161 Size: 932 229 NaeI GCC'GGC Cuts at: 0 301 1161 Size: 301 860 NcoI C'CATG_G Cuts at: 0 1054 1161 Size: 1054 107 NgoMIV G'CCGG_C Cuts at: 0 299 1161 Size: 299 862 NspI r_CATG'y Cuts at: 0 670 864 1161 Size: 670 194 297 Fragments arranged by size: 670 297 194 PasI CC'CwG_GG Cuts at: 0 600 1161 Size: 600 561 PfoI T'CCnGG_A Cuts at: 0 172 581 1161 Size: 172 409 580 Fragments arranged by size: 580 409 172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 400 432 1161 Size: 400 32 729 Fragments arranged by size: 729 400 32 PpuMI rG'GwC_Cy Cuts at: 0 999 1161 Size: 999 162 PspOMI G'GGCC_C Cuts at: 0 1074 1161 Size: 1074 87 PstI C_TGCA'G Cuts at: 0 20 1161 Size: 20 1141 SacI G_AGCT'C Cuts at: 0 992 1161 Size: 992 169 SanDI GG'GwC_CC Cuts at: 0 999 1161 Size: 999 162 SapI GCTCTTCn'nnn_ Cuts at: 0 316 1161 Size: 316 845 SfcI C'TryA_G Cuts at: 0 16 1161 Size: 16 1145 SgrAI Cr'CCGG_yG Cuts at: 0 93 1161 Size: 93 1068 SmlI C'TyrA_G Cuts at: 0 166 1161 Size: 166 995 SphI G_CATG'C Cuts at: 0 670 864 1161 Size: 670 194 297 Fragments arranged by size: 670 297 194 StyI C'CwwG_G Cuts at: 0 80 1054 1161 Size: 80 974 107 Fragments arranged by size: 974 107 80 TatI w'GTAC_w Cuts at: 0 878 1161 Size: 878 283 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 154 186 1161 Size: 154 32 975 Fragments arranged by size: 975 154 32 Tth111I GACn'n_nGTC Cuts at: 0 1109 1161 Size: 1109 52 Enzymes that do cut: AccI AcuI AleI AlfI AloI AlwNI ApaI BamHI BanI BanII BcgI BclI BglII BlpI Bme1580I BpmI BpuEI BsaBI BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BssSI BstAPI BstYI BtgI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII MfeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PfoI PpiI PpuMI PspOMI PstI SacI SanDI SapI SfcI SgrAI SmlI SphI StyI TatI TstI Tth111I Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI BciVI BfrBI BglI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII XbaI XcmI XhoI XmaI XmnI ZraI