################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HindIII KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI StuI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 1229 GACGTC GACGT^C AcsI 940 AAATTT [GA]^AATT[CT] AflIII 101 ACGTGT A^C[GA][CT]GT ApoI 940 AAATTT [GA]^AATT[CT] Asp700I 1293 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1576 CCCGGG C^[CT]CG[GA]G BalI 824 TGGCCA TGG^CCA BciVI 776 GGATAC GTATCC(6/5) BglI 745 GCCGAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseRI 1484 GAGGAG GAGGAG(10/8) BsoBI 1576 CCCGGG C^[CT]CG[GA]G BspDI 1783 ATCGAT AT^CGAT BsrFI 717 GCCGGT [GA]^CCGG[CT] BstXI 1472 CCAAGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 717 GCCGGT [GA]^CCGG[CT] ClaI 1783 ATCGAT AT^CGAT DraIII 292 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 821 CCATGG C^C[GA][CT]GG EagI 1142 CGGCCG C^GGCCG EclXI 1142 CGGCCG C^GGCCG Eco52I 1142 CGGCCG C^GGCCG EcoT14I 821 CCATGG C^C[AT][AT]GG EcoT22I 872 ATGCAT ATGCA^T HaeII 1177 AGCGCC [GA]GCGC^[CT] HpaI 841 GTTAAC GTT^AAC HphI 1055 TCACC GGTGA(8/7) MluNI 824 TGGCCA TGG^CCA MscI 824 TGGCCA TGG^CCA NcoI 821 CCATGG C^CATGG NsiI 872 ATGCAT ATGCA^T PpuMI 379 AGGACCT [GA]G^G[AT]CC[CT] PstI 815 CTGCAG CTGCA^G PvuII 493 CAGCTG CAG^CTG ScaI 1766 AGTACT AGT^ACT SmaI 1576 CCCGGG CCC^GGG SmlI 1642 CTTGAG C^T[CT][GA]AG SpeI 786 ACTAGT A^CTAGT StyI 821 CCATGG C^C[AT][AT]GG XmaCI 1576 CCCGGG C^CCGGG XmaI 1576 CCCGGG C^CCGGG XmnI 1293 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AcyI 332 GACGCC G[GA]^CG[CT]C 1229 GACGTC AlwI 416 GATCC GGATC(4/5) 585 GATCC AocI 448 CCTAAGG CC^T[ACGT]AGG 793 CCTCAGG ApaI 66 GGGCCC GGGCC^C 735 GGGCCC AspI 661 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1436 GACAGAGTC BbsI 225 GAAGAC GAAGAC(2/6) 1192 GTCTTC BlpI 833 GCTTAGC GC^T[ACGT]AGC 1173 GCTCAGC Bpu1102I 833 GCTTAGC GC^T[ACGT]AGC 1173 GCTCAGC BpuAI 225 GAAGAC GAAGAC(2/6) 1192 GTCTTC BsaHI 332 GACGCC G[GA]^CG[CT]C 1229 GACGTC BsaI 959 GAGACC GGTCTC(1/5) 1557 GGTCTC BsiEI 719 CGGTCG CG[GA][CT]^CG 1142 CGGCCG BsmAI 959 GAGAC GTCTC(1/5) 1558 GTCTC BsmFI 889 GGGAC GGGAC(10/14) 1579 GGGAC BstYI 415 AGATCC [GA]^GATC[CT] 584 AGATCC Bsu36I 448 CCTAAGG CC^T[ACGT]AGG 793 CCTCAGG CelII 833 GCTTAGC GC^T[ACGT]AGC 1173 GCTCAGC EaeI 824 TGGCCA [CT]^GGCC[GA] 1142 CGGCCG EarI 90 GAAGAG CTCTTC(1/4) 956 GAAGAG Eco81I 448 CCTAAGG CC^T[ACGT]AGG 793 CCTCAGG FauI 175 CCCGC CCCGC(4/6) 158 GCGGG HgaI 332 GACGC GACGC(5/10) 1190 GCGTC Hin1I 332 GACGCC G[GA]^CG[CT]C 1229 GACGTC HincII 199 GTTGAC GT[CT]^[GA]AC 841 GTTAAC HindII 199 GTTGAC GT[CT]^[GA]AC 841 GTTAAC Ksp632I 90 GAAGAG CTCTTC(1/4) 956 GAAGAG MflI 415 AGATCC [GA]^GATC[CT] 584 AGATCC PflFI 661 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1436 GACAGAGTC PflMI 1342 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1743 CCAGGGGATGG PleI 1753 GACTC GAGTC(4/5) 1440 GAGTC SfcI 815 CTGCAG C^T[GA][CT]AG 1014 CTACAG TaqI 668 TCGA T^CGA 1784 TCGA TfiI 478 GAATC G^A[AT]TC 1648 GAATC Tsp45I 811 GTGAC ^GT[GC]AC 922 GTGAC Tsp509I 941 AATT ^AATT 1307 AATT Tth111I 661 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 1436 GACAGAGTC TthHB8I 668 TCGA T^CGA 1784 TCGA Van91I 1342 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1743 CCAGGGGATGG XhoII 415 AGATCC [GA]^GATC[CT] 584 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BssSI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI TspRI