Restriction map for
pFN21AE1167
(Circular) (Six-base) MAPSORT of: pFN21AE1167.seq Check: 6254 from: 1 to: 6499
pFN21AE1167 6499 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 4309 278
Size: 53 83 186 936 1702 1071 2468
Fragments arranged by size:
2468 1702 1071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3842 1937
Size: 1905 4594
Acc65I G'GTAC_C
Cuts at: 3820 3820
Size: 6499
AclI AA'CG_TT
Cuts at: 1563 4626 4999 1563
Size: 3063 373 3063
Fragments arranged by size:
3063 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2112 2114 3746 4562 5946 6214 1632
Size: 480 2 1632 816 1384 268 1917
Fragments arranged by size:
1917 1632 1384 816 480 268 2
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6302
AflIII A'CryG_T
Cuts at: 1203 2106 5398 6375 1203
Size: 903 3292 977 1327
Fragments arranged by size:
3292 1327 977 903
AgeI A'CCGG_T
Cuts at: 5330 5330
Size: 6499
AhdI GACnn_n'nnGTC
Cuts at: 5228 5228
Size: 6499
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6499
AlwNI CAG_nnn'CTG
Cuts at: 4242 5814 4242
Size: 1572 4927
ApaI G_GGCC'C
Cuts at: 2075 2744 2075
Size: 669 5830
ApaLI G'TGCA_C
Cuts at: 4556 5712 4556
Size: 1156 5343
ApoI r'AATT_y
Cuts at: 1493 2945 3808 4034 4186 6295 1493
Size: 1452 863 226 152 2109 1697
Fragments arranged by size:
2109 1697 1452 863 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5053 160
Size: 872 4021 1606
Fragments arranged by size:
4021 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
AvaI C'yCGr_G
Cuts at: 1870 1943 3581 3824 1870
Size: 73 1638 243 4545
Fragments arranged by size:
4545 1638 243 73
BamHI G'GATC_C
Cuts at: 3829 4196 3829
Size: 367 6132
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2698 2747 2803 3820 5275
Size: 325 217 627 911 49 56 1017 1455
Cuts at: 5275 618
Size: 1842
Fragments arranged by size:
1842 1455 1017 911 627 325 217 56
49
BanII G_rGCy'C
Cuts at: 1391 2075 2654 2744 3743 3818 1391
Size: 684 579 90 999 75 4072
Fragments arranged by size:
4072 999 684 579 90 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2237 3190 928
Size: 971 338 953 4237
Fragments arranged by size:
4237 971 953 338
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4690 4724 1903
Size: 34 2753 34 3678
Fragments arranged by size:
3678 2753 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2775 4391 5601 2775
Size: 1616 1210 3673
Fragments arranged by size:
3673 1616 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6499
BfrBI ATG'CAT
Cuts at: 2879 2879
Size: 6499
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2756 5110 136
Size: 107 122 71 631 605 1084 2354 1525
Fragments arranged by size:
2354 1525 1084 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6494 6494
Size: 6499
BlpI GC'TnA_GC
Cuts at: 2839 3179 3916 2839
Size: 340 737 5422
Fragments arranged by size:
5422 737 340
Bme1580I G_kGCm'C
Cuts at: 1790 2075 2170 2744 2808 4560 5716 1790
Size: 285 95 574 64 1752 1156 2573
Fragments arranged by size:
2573 1752 1156 574 285 95 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6499
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4230 5188 442
Size: 416 53 83 399 24 2813 958 1753
Fragments arranged by size:
2813 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6499
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3115 3643 3115
Size: 528 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2401 2533 3713 5159 1239
Size: 1162 132 1180 1446 2579
Fragments arranged by size:
2579 1446 1180 1162 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3668 4624 5489 5787 3668
Size: 956 865 298 4380
Fragments arranged by size:
4380 956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2958 3568 5162 882
Size: 617 1459 610 1594 2219
Fragments arranged by size:
2219 1594 1459 617 610
BsaAI yAC'GTr
Cuts at: 493 1204 4290 493
Size: 711 3086 2702
Fragments arranged by size:
3086 2702 711
BsaBI GATnn'nnATC
Cuts at: 6493 6493
Size: 6499
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2338 3235 4306 4688
Size: 53 83 186 936 805 897 1071 382
Cuts at: 4688 5323 275
Size: 635 1451
Fragments arranged by size:
1451 1071 936 897 805 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4932 5330 5604 5751 6162 4932
Size: 398 274 147 411 5269
Fragments arranged by size:
5269 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6274
Fragments arranged by size:
6274 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3504 1186
Size: 2318 4181
BseYI C'CCAG_C
Cuts at: 2135 3346 5702 6198 2135
Size: 1211 2356 496 2436
Fragments arranged by size:
2436 2356 1211 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5926
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2727 3150 3949 4710 5738 1540
Size: 275 186 726 423 799 761 1028 2301
Fragments arranged by size:
2301 1028 799 761 726 423 275 186
BsiHKAI G_wGCw'C
Cuts at: 2366 3139 3146 4560 4645 5716 2366
Size: 773 7 1414 85 1071 3149
Fragments arranged by size:
3149 1414 1071 773 85 7
BsmI GAATG_Cn'
Cuts at: 4010 4103 4010
Size: 93 6406
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2170 2366 2654 2744 2808 3139
Size: 399 285 95 196 288 90 64 331
Cuts at: 3139 3146 3743 3818 4560 4645 5716 1391
Size: 7 597 75 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 597 399 331 288 285
196 95 90 85 75 64 7
BspEI T'CCGG_A
Cuts at: 6162 6162
Size: 6499
BspHI T'CATG_A
Cuts at: 4388 6118 4388
Size: 1730 4769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3854 844
Size: 3010 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4386 1394
Size: 429 2563 3507
Fragments arranged by size:
3507 2563 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4994 5168 65
Size: 1183 3746 174 1396
Fragments arranged by size:
3746 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2722 5143 5330 1700
Size: 110 912 2421 187 2869
Fragments arranged by size:
2869 2421 912 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6499
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6499
BssSI C'ACGA_G
Cuts at: 2300 2462 2594 3628 3665 4559 5571 2300
Size: 162 132 1034 37 894 1012 3228
Fragments arranged by size:
3228 1034 1012 894 162 132 37
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6499
BstBI TT'CG_AA
Cuts at: 5303 5303
Size: 6499
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6499
BstXI CCAn_nnnn'nTGG
Cuts at: 3484 3484
Size: 6499
BstYI r'GATC_y
Cuts at: 1972 2420 2589 3829 4196 4581 4598 6050 6136
Size: 448 169 1240 367 385 17 1452 86
Cuts at: 6136 6148 6494 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1240 448 385 367 346 169
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2454 2799 2454
Size: 345 6154
BtgI C'CryG_G
Cuts at: 513 1065 2826 513
Size: 552 1761 4186
Fragments arranged by size:
4186 1761 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3399 487
Size: 683 56 2173 3587
Fragments arranged by size:
3587 2173 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 2634 4015 4809 4829 2158
Size: 476 1381 794 20 3828
Fragments arranged by size:
3828 1381 794 476 20
ClaI AT'CG_AT
Cuts at: 1554 3789 1554
Size: 2235 4264
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6464
DraI TTT'AAA
Cuts at: 3803 3803
Size: 6499
DraIII CAC_nnn'GTG
Cuts at: 2302 2302
Size: 6499
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5506 809
Size: 507 4190 1802
Fragments arranged by size:
4190 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2829 3147 3946
Size: 54 1425 50 275 18 999 318 799
Cuts at: 3946 4835 6464 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 999 889 799 318 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3147 3946 1537
Size: 275 1335 799 4090
Fragments arranged by size:
4090 1335 799 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2956 4429 1353
Size: 737 866 1473 3423
Fragments arranged by size:
3423 1473 866 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5086 5460 5606 387
Size: 1064 138 3497 374 146 1280
Fragments arranged by size:
3497 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2114 2401 2533 3713 3746 4562
Size: 393 480 2 287 132 1180 33 816
Cuts at: 4562 5159 5946 6214 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1180 816 787 597 480 393 287
268 132 33 2
EcoO109I rG'GnC_Cy
Cuts at: 2071 2385 2741 3556 2071
Size: 314 356 815 5014
Fragments arranged by size:
5014 815 356 314
EcoRI G'AATT_C
Cuts at: 3808 4186 3808
Size: 378 6121
FspI TGC'GCA
Cuts at: 5005 5005
Size: 6499
HaeII r_GCGC'y
Cuts at: 1678 3186 5646 1678
Size: 1508 2460 2531
Fragments arranged by size:
2531 2460 1508
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 3577 3609 5140
Size: 32 135 32 896 32 1077 32 1531
Cuts at: 5140 5172 5214 5246 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1531 1077 896 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2206 2848 3843 4089 669
Size: 988 281 268 642 995 246 3079
Fragments arranged by size:
3079 995 988 642 281 268 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6499
HpaI GTT'AAC
Cuts at: 2848 4089 2848
Size: 1241 5258
KpnI G_GTAC'C
Cuts at: 3824 3824
Size: 6499
MfeI C'AATT_G
Cuts at: 4098 4098
Size: 6499
MluI A'CGCG_T
Cuts at: 6375 6375
Size: 6499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2707 5613 5797 1305
Size: 1402 2906 184 2007
Fragments arranged by size:
2906 2007 1402 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2831 10
Size: 54 1425 343 999 3678
Fragments arranged by size:
3678 1425 999 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 3269 3683 4457 4816 4975 518
Size: 648 978 1125 414 774 359 159 2042
Fragments arranged by size:
2042 1125 978 774 648 414 359 159
MspA1I CmG'CkG
Cuts at: 1606 2500 2714 3152 3915 4592 5740 5985 1606
Size: 894 214 438 763 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 763 677 438 245 214
NcoI C'CATG_G
Cuts at: 513 1065 2826 513
Size: 552 1761 4186
Fragments arranged by size:
4186 1761 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6499
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6499
NotI GC'GGCC_GC
Cuts at: 3946 3946
Size: 6499
NsiI A_TGCA'T
Cuts at: 2881 2881
Size: 6499
NspI r_CATG'y
Cuts at: 1132 1229 3857 5402 1132
Size: 97 2628 1545 2229
Fragments arranged by size:
2628 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3748 1348
Size: 2400 4099
PciI A'CATG_T
Cuts at: 5398 5398
Size: 6499
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3353 3754 1108
Size: 2245 401 3853
Fragments arranged by size:
3853 2245 401
PmeI GTTT'AAAC
Cuts at: 3803 3803
Size: 6499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2283 2315 4568 4600 6081 6113 2283
Size: 32 2253 32 1481 32 2669
Fragments arranged by size:
2669 2253 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2385 2385
Size: 6499
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6499
PsiI TTA'TAA
Cuts at: 4069 4069
Size: 6499
PspOMI G'GGCC_C
Cuts at: 2071 2740 2071
Size: 669 5830
PstI C_TGCA'G
Cuts at: 830 2824 3851 830
Size: 1994 1027 3478
Fragments arranged by size:
3478 1994 1027
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6499
PvuII CAG'CTG
Cuts at: 2500 2500
Size: 6499
SalI G'TCGA_C
Cuts at: 1936 3841 1936
Size: 1905 4594
SbfI CC_TGCA'GG
Cuts at: 3851 3851
Size: 6499
ScaI AGT'ACT
Cuts at: 3773 4747 6459 3773
Size: 974 1712 3813
Fragments arranged by size:
3813 1712 974
SfcI C'TryA_G
Cuts at: 826 1045 2820 3019 3847 4982 5663 5854 826
Size: 219 1775 199 828 1135 681 191 1471
Fragments arranged by size:
1775 1471 1135 828 681 219 199 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6499
SmaI CCC'GGG
Cuts at: 1872 3583 3826 1872
Size: 1711 243 4545
Fragments arranged by size:
4545 1711 243
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3647 4603 5504 5766 820
Size: 197 926 1704 956 901 262 1553
Fragments arranged by size:
1704 1553 956 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4290 493
Size: 3797 2702
SpeI A'CTAG_T
Cuts at: 152 2791 152
Size: 2639 3860
SphI G_CATG'C
Cuts at: 1132 3857 1132
Size: 2725 3774
SspI AAT'ATT
Cuts at: 5 52 4423 5
Size: 47 4371 2081
Fragments arranged by size:
4371 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6499
StyI C'CwwG_G
Cuts at: 513 1065 2826 3938 513
Size: 552 1761 1112 3074
Fragments arranged by size:
3074 1761 1112 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2802 3077 4526 4543 4696 4881 5326
Size: 1255 1222 275 1449 17 153 185 445
Cuts at: 5326 325
Size: 1498
Fragments arranged by size:
1498 1449 1255 1222 445 275 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3771 4745 6457
Size: 274 80 33 51 1443 1794 974 1712
Cuts at: 6457 96
Size: 138
Fragments arranged by size:
1794 1712 1443 974 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6459 6491 6459
Size: 32 6467
Tth111I GACn'n_nGTC
Cuts at: 1316 2669 3444 1316
Size: 1353 775 4371
Fragments arranged by size:
4371 1353 775
XbaI T'CTAG_A
Cuts at: 3835 3835
Size: 6499
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6499
XmaI C'CCGG_G
Cuts at: 1870 3581 3824 1870
Size: 1711 243 4545
Fragments arranged by size:
4545 1711 243
XmnI GAAnn'nnTTC
Cuts at: 3302 4628 3302
Size: 1326 5173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 4307 276
Size: 53 83 186 936 1702 1071 2468
Fragments arranged by size:
2468 1702 1071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI