(Linear) (Six-base) MAPSORT of: KIEE1167.seq Check: 6685 from: 1 to: 1794 KIEE1167.orf With 182 enzymes: SgfI * January 29, 2014 11:44 .. AatII G_ACGT'C Cuts at: 0 1233 1794 Size: 1233 561 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 107 109 1741 1794 Size: 107 2 1632 53 Fragments arranged by size: 1632 107 53 2 AflIII A'CryG_T Cuts at: 0 101 1794 Size: 101 1693 ApaI G_GGCC'C Cuts at: 0 70 739 1794 Size: 70 669 1055 Fragments arranged by size: 1055 669 70 ApoI r'AATT_y Cuts at: 0 940 1794 Size: 940 854 AvaI C'yCGr_G Cuts at: 0 1576 1794 Size: 1576 218 BanI G'GyrC_C Cuts at: 0 693 742 798 1794 Size: 693 49 56 996 Fragments arranged by size: 996 693 56 49 BanII G_rGCy'C Cuts at: 0 70 649 739 1738 1794 Size: 70 579 90 999 56 Fragments arranged by size: 999 579 90 70 56 BbsI GAAGACnn'nnnn_ Cuts at: 0 232 1185 1794 Size: 232 953 609 Fragments arranged by size: 953 609 232 BciVI GTATCCnnnnn_n' Cuts at: 0 770 1794 Size: 770 1024 BfrBI ATG'CAT Cuts at: 0 874 1794 Size: 874 920 BglI GCCn_nnn'nGGC Cuts at: 0 751 1794 Size: 751 1043 BlpI GC'TnA_GC Cuts at: 0 834 1174 1794 Size: 834 340 620 Fragments arranged by size: 834 620 340 Bme1580I G_kGCm'C Cuts at: 0 70 165 739 803 1794 Size: 70 95 574 64 991 Fragments arranged by size: 991 574 95 70 64 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1110 1638 1794 Size: 1110 528 156 Fragments arranged by size: 1110 528 156 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 396 528 1708 1794 Size: 396 132 1180 86 Fragments arranged by size: 1180 396 132 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1663 1794 Size: 1663 131 BsaI GGTCTCn'nnnn_ Cuts at: 0 953 1563 1794 Size: 953 610 231 Fragments arranged by size: 953 610 231 BsaHI Gr'CG_yC Cuts at: 0 333 1230 1794 Size: 333 897 564 Fragments arranged by size: 897 564 333 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1499 1794 Size: 1499 295 BseYI C'CCAG_C Cuts at: 0 130 1341 1794 Size: 130 1211 453 Fragments arranged by size: 1211 453 130 BsiEI CG_ry'CG Cuts at: 0 722 1145 1794 Size: 722 423 649 Fragments arranged by size: 722 649 423 BsiHKAI G_wGCw'C Cuts at: 0 361 1134 1141 1794 Size: 361 773 7 653 Fragments arranged by size: 773 653 361 7 Bsp1286I G_dGCh'C Cuts at: 0 70 165 361 649 739 803 1134 1141 Size: 70 95 196 288 90 64 331 7 Cuts at: 1141 1738 1794 Size: 597 56 Fragments arranged by size: 597 331 288 196 95 90 70 64 56 7 BsrFI r'CCGG_y Cuts at: 0 717 1794 Size: 717 1077 BssSI C'ACGA_G Cuts at: 0 295 457 589 1623 1660 1794 Size: 295 162 132 1034 37 134 Fragments arranged by size: 1034 295 162 134 132 37 BstXI CCAn_nnnn'nTGG Cuts at: 0 1479 1794 Size: 1479 315 BstYI r'GATC_y Cuts at: 0 415 584 1794 Size: 415 169 1210 Fragments arranged by size: 1210 415 169 Bsu36I CC'TnA_GG Cuts at: 0 449 794 1794 Size: 449 345 1000 Fragments arranged by size: 1000 449 345 BtgI C'CryG_G Cuts at: 0 821 1794 Size: 821 973 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1394 1794 Size: 1394 400 BtsI GCAGTG_nn' Cuts at: 0 153 629 1794 Size: 153 476 1165 Fragments arranged by size: 1165 476 153 ClaI AT'CG_AT Cuts at: 0 1784 1794 Size: 1784 10 DraIII CAC_nnn'GTG Cuts at: 0 297 1794 Size: 297 1497 EaeI y'GGCC_r Cuts at: 0 824 1142 1794 Size: 824 318 652 Fragments arranged by size: 824 652 318 EagI C'GGCC_G Cuts at: 0 1142 1794 Size: 1142 652 EarI CTCTTCn'nnn_ Cuts at: 0 85 951 1794 Size: 85 866 843 Fragments arranged by size: 866 843 85 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 107 109 396 528 1708 1741 1794 Size: 107 2 287 132 1180 33 53 Fragments arranged by size: 1180 287 132 107 53 33 2 EcoO109I rG'GnC_Cy Cuts at: 0 66 380 736 1551 1794 Size: 66 314 356 815 243 Fragments arranged by size: 815 356 314 243 66 HaeII r_GCGC'y Cuts at: 0 1181 1794 Size: 1181 613 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 463 495 1572 1604 1794 Size: 463 32 1077 32 190 Fragments arranged by size: 1077 463 190 32 32 HincII GTy'rAC Cuts at: 0 201 843 1794 Size: 201 642 951 Fragments arranged by size: 951 642 201 HpaI GTT'AAC Cuts at: 0 843 1794 Size: 843 951 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 702 1794 Size: 702 1092 MscI TGG'CCA Cuts at: 0 826 1794 Size: 826 968 MslI CAynn'nnrTG Cuts at: 0 139 1264 1678 1794 Size: 139 1125 414 116 Fragments arranged by size: 1125 414 139 116 MspA1I CmG'CkG Cuts at: 0 495 709 1147 1794 Size: 495 214 438 647 Fragments arranged by size: 647 495 438 214 NcoI C'CATG_G Cuts at: 0 821 1794 Size: 821 973 NsiI A_TGCA'T Cuts at: 0 876 1794 Size: 876 918 PasI CC'CwG_GG Cuts at: 0 1743 1794 Size: 1743 51 PflMI CCAn_nnn'nTGG Cuts at: 0 1348 1749 1794 Size: 1348 401 45 Fragments arranged by size: 1348 401 45 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 278 310 1794 Size: 278 32 1484 Fragments arranged by size: 1484 278 32 PpuMI rG'GwC_Cy Cuts at: 0 380 1794 Size: 380 1414 PspOMI G'GGCC_C Cuts at: 0 66 735 1794 Size: 66 669 1059 Fragments arranged by size: 1059 669 66 PstI C_TGCA'G Cuts at: 0 819 1794 Size: 819 975 PvuII CAG'CTG Cuts at: 0 495 1794 Size: 495 1299 ScaI AGT'ACT Cuts at: 0 1768 1794 Size: 1768 26 SfcI C'TryA_G Cuts at: 0 815 1014 1794 Size: 815 199 780 Fragments arranged by size: 815 780 199 SmaI CCC'GGG Cuts at: 0 1578 1794 Size: 1578 216 SmlI C'TyrA_G Cuts at: 0 1642 1794 Size: 1642 152 SpeI A'CTAG_T Cuts at: 0 786 1794 Size: 786 1008 StyI C'CwwG_G Cuts at: 0 821 1794 Size: 821 973 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 797 1072 1794 Size: 797 275 722 Fragments arranged by size: 797 722 275 TatI w'GTAC_w Cuts at: 0 1766 1794 Size: 1766 28 Tth111I GACn'n_nGTC Cuts at: 0 664 1439 1794 Size: 664 775 355 Fragments arranged by size: 775 664 355 XmaI C'CCGG_G Cuts at: 0 1576 1794 Size: 1576 218 XmnI GAAnn'nnTTC Cuts at: 0 1297 1794 Size: 1297 497 ZraI GAC'GTC Cuts at: 0 1231 1794 Size: 1231 563 Enzymes that do cut: AatII AcuI AflIII ApaI ApoI AvaI BanI BanII BbsI BciVI BfrBI BglI BlpI Bme1580I BplI BpmI BpuEI BsaI BsaHI BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrFI BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII EaeI EagI EarI Eco57MI EcoO109I HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NcoI NsiI PasI PflMI PpiI PpuMI PspOMI PstI PvuII ScaI SfcI SmaI SmlI SpeI StyI TaqII TatI Tth111I XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BglII BmgBI BmrI BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI