Restriction map for
pFN21AE0978
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BbsI BciVI BclI BfrI
BpuAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 1562 GGCGCC G[GA]^CG[CT]C
Aor51HI 1194 AGCGCT AGC^GCT
ApaI 1018 GGGCCC GGGCC^C
AspHI 429 GAGCTC G[AT]GC[AT]^C
AvrII 841 CCTAGG C^CTAGG
BbeI 1562 GGCGCC GGCGC^C
BbrPI 79 CACGTG CAC^GTG
BbvCI 447 GCTGAGG CCTCAGC(-5/-2)
BcgI 458 GCAGGGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 335 AGATCT A^GATCT
BlnI 841 CCTAGG C^CTAGG
BsaAI 79 CACGTG [CT]AC^GT[GA]
BsaHI 1562 GGCGCC G[GA]^CG[CT]C
BsaI 1412 GGTCTC GGTCTC(1/5)
BsaWI 508 ACCGGA [AT]^CCGG[AT]
BsiEI 1119 CGGCCG CG[GA][CT]^CG
BsiHKAI 429 GAGCTC G[AT]GC[AT]^C
BsmAI 1413 GTCTC GTCTC(1/5)
BsrBI 562 GAGCGG CCGCTC(-3/-3)
BssSI 1210 CACGAG CACGAG(-5/-1)
BstXI 804 CCATGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 547 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1119 CGGCCG C^GGCCG
EarI 528 GAAGAG CTCTTC(1/4)
EclXI 1119 CGGCCG C^GGCCG
Eco47III 1194 AGCGCT AGC^GCT
Eco52I 1119 CGGCCG C^GGCCG
EcoT14I 841 CCTAGG C^C[AT][AT]GG
HgaI 1012 GACGC GACGC(5/10)
Hin1I 1562 GGCGCC G[GA]^CG[CT]C
HincII 265 GTTGAC GT[CT]^[GA]AC
HindII 265 GTTGAC GT[CT]^[GA]AC
KasI 1562 GGCGCC G^GCGCC
Ksp632I 528 GAAGAG CTCTTC(1/4)
NarI 1562 GGCGCC GG^CGCC
NspI 654 GCATGT [GA]CATG^[CT]
PflMI 1622 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 79 CACGTG CAC^GTG
PmlI 79 CACGTG CAC^GTG
SacI 429 GAGCTC GAGCT^C
SapI 528 GAAGAGC GCTCTTC(1/4)
SfoI 1562 GGCGCC GGC^GCC
SmaI 1243 CCCGGG CCC^GGG
SmlI 1597 CTCAAG C^T[CT][GA]AG
StyI 841 CCTAGG C^C[AT][AT]GG
Tsp509I 306 AATT ^AATT
Van91I 1622 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1243 CCCGGG C^CCGGG
XmaI 1243 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 187 CGCG CG^CG
387 CGCG
AflIII 80 ACGTGT A^C[GA][CT]GT
261 ACACGT
AlwI 168 GATCC GGATC(4/5)
1089 GGATC
AlwNI 355 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1010 CAGACGCTG
BanI 542 GGTGCC G^G[CT][GA]CC
1562 GGCGCC
BglI 1497 GCCCGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1554 GCCTACCCGGC
BsrFI 1054 GCCGGC [GA]^CCGG[CT]
1117 GCCGGC
BstAPI 354 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1215 GCAGGGGGTGC
BstUI 187 CGCG CG^CG
387 CGCG
BstYI 167 AGATCC [GA]^GATC[CT]
335 AGATCT
Cfr10I 1054 GCCGGC [GA]^CCGG[CT]
1117 GCCGGC
EcoNI 951 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1257 CCTGGACCAGG
FseI 1053 GGCCGGCC GGCCGG^CC
1116 GGCCGGCC
MflI 167 AGATCC [GA]^GATC[CT]
335 AGATCT
MvnI 187 CGCG CG^CG
387 CGCG
NaeI 1054 GCCGGC GCC^GGC
1117 GCCGGC
NgoMIV 1054 GCCGGC G^CCGGC
1117 GCCGGC
PleI 631 GACTC GAGTC(4/5)
587 GAGTC
SfaNI 154 GATGC GCATC(5/9)
1185 GATGC
TaqI 476 TCGA T^CGA
1550 TCGA
TthHB8I 476 TCGA T^CGA
1550 TCGA
XhoII 167 AGATCC [GA]^GATC[CT]
335 AGATCT
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AocI AvaI
AvaII BalI BanII BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI TfiI TseI Tsp45I
TspRI XcmI