(Circular) (Six-base) MAPSORT of: pFN21AE0978.seq Check: 7751 from: 1 to: 6352 pFN21AE0978 6352 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2364 2697 2988 3157 2364 Size: 333 291 169 5559 Fragments arranged by size: 5559 333 291 169 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4162 278 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3695 1937 Size: 1758 4594 Acc65I G'GTAC_C Cuts at: 3673 3673 Size: 6352 AclI AA'CG_TT Cuts at: 1563 4479 4852 1563 Size: 2916 373 3063 Fragments arranged by size: 3063 2916 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2931 3453 4415 5799 6067 1632 Size: 1299 522 962 1384 268 1917 Fragments arranged by size: 1917 1384 1299 962 522 268 AfeI AGC'GCT Cuts at: 3201 3201 Size: 6352 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6155 AflIII A'CryG_T Cuts at: 1203 2085 2266 5251 6228 1203 Size: 882 181 2985 977 1327 Fragments arranged by size: 2985 1327 977 882 181 AgeI A'CCGG_T Cuts at: 5183 5183 Size: 6352 AhdI GACnn_n'nnGTC Cuts at: 5081 5081 Size: 6352 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6352 AlwNI CAG_nnn'CTG Cuts at: 2365 3020 4095 5667 2365 Size: 655 1075 1572 3050 Fragments arranged by size: 3050 1572 1075 655 ApaI G_GGCC'C Cuts at: 3027 3027 Size: 6352 ApaLI G'TGCA_C Cuts at: 4409 5565 4409 Size: 1156 5196 ApoI r'AATT_y Cuts at: 1493 3661 3887 4039 6148 1493 Size: 2168 226 152 2109 1697 Fragments arranged by size: 2168 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4906 160 Size: 872 3874 1606 Fragments arranged by size: 3874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 3030 3248 3503 3677 1870 Size: 73 1087 218 255 174 4545 Fragments arranged by size: 4545 1087 255 218 174 73 AvrII C'CTAG_G Cuts at: 2846 2846 Size: 6352 BamHI G'GATC_C Cuts at: 3682 4049 3682 Size: 367 5985 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2547 3567 3673 5128 618 Size: 325 217 627 760 1020 106 1455 1842 Fragments arranged by size: 1842 1455 1020 760 627 325 217 106 BanII G_rGCy'C Cuts at: 1391 2438 2724 3027 3039 3321 3671 1391 Size: 1047 286 303 12 282 350 4072 Fragments arranged by size: 4072 1047 350 303 286 282 12 BbeI G_GCGC'C Cuts at: 3571 3571 Size: 6352 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5381 BbvCI CC'TCA_GC Cuts at: 2453 2453 Size: 6352 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2452 2486 4543 4577 1903 Size: 34 515 34 2057 34 3678 Fragments arranged by size: 3678 2057 515 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4244 5454 4244 Size: 1210 5142 BclI T'GATC_A Cuts at: 1548 1548 Size: 6352 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3508 3565 4963 Size: 107 122 71 631 605 1836 57 1398 Cuts at: 4963 136 Size: 1525 Fragments arranged by size: 1836 1525 1398 631 605 122 107 71 57 BglII A'GATC_T Cuts at: 2340 6347 2340 Size: 4007 2345 BlpI GC'TnA_GC Cuts at: 2369 2432 2723 2757 3769 2369 Size: 63 291 34 1012 4952 Fragments arranged by size: 4952 1012 291 63 34 Bme1580I G_kGCm'C Cuts at: 1790 3027 4413 5569 1790 Size: 1237 1386 1156 2573 Fragments arranged by size: 2573 1386 1237 1156 BmgBI CAC'GTC Cuts at: 1318 2248 2291 1318 Size: 930 43 5379 Fragments arranged by size: 5379 930 43 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3316 4083 5041 Size: 416 53 83 399 24 1899 767 958 Cuts at: 5041 442 Size: 1753 Fragments arranged by size: 1899 1753 958 767 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6352 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2077 2693 2077 Size: 616 5736 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2114 2207 2675 2858 3169 3224 3479 Size: 827 48 93 468 183 311 55 255 Cuts at: 3479 5012 1239 Size: 1533 2579 Fragments arranged by size: 2579 1533 827 468 311 255 183 93 55 48 Bpu10I CC'TnA_GC Cuts at: 2052 2453 2615 2052 Size: 401 162 5789 Fragments arranged by size: 5789 401 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3587 4477 5342 5640 3587 Size: 890 865 298 4299 Fragments arranged by size: 4299 890 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3423 5015 882 Size: 617 1924 1592 2219 Fragments arranged by size: 2219 1924 1592 617 BsaAI yAC'GTr Cuts at: 493 1204 2086 4143 493 Size: 711 882 2057 2702 Fragments arranged by size: 2702 2057 882 711 BsaBI GATnn'nnATC Cuts at: 6346 6346 Size: 6352 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 4159 4541 5176 Size: 53 83 186 936 2035 591 382 635 Cuts at: 5176 275 Size: 1451 Fragments arranged by size: 2035 1451 936 635 591 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2513 4785 5183 5457 5604 6015 2513 Size: 2272 398 274 147 411 2850 Fragments arranged by size: 2850 2272 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2678 2708 3308 3338 1178 Size: 30 165 30 1275 30 600 30 4192 Fragments arranged by size: 4192 1275 600 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 2938 3176 1186 Size: 1165 587 238 4362 Fragments arranged by size: 4362 1165 587 238 BseYI C'CCAG_C Cuts at: 2033 2387 2788 2873 3020 3489 3520 5555 6051 Size: 354 401 85 147 469 31 2035 496 Cuts at: 6051 2033 Size: 2334 Fragments arranged by size: 2334 2035 496 469 401 354 147 85 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2510 2633 2966 3197 1163 Size: 573 774 123 333 231 4318 Fragments arranged by size: 4318 774 573 333 231 123 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3802 4563 5591 1540 Size: 275 186 1126 675 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 675 275 186 BsiHKAI G_wGCw'C Cuts at: 2438 4413 4498 5569 2438 Size: 1975 85 1071 3221 Fragments arranged by size: 3221 1975 1071 85 BsmI GAATG_Cn' Cuts at: 3863 3956 3863 Size: 93 6259 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2438 2724 3027 3039 3321 3671 4413 Size: 399 648 286 303 12 282 350 742 Cuts at: 4413 4498 5569 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 648 399 350 303 286 282 85 12 BspEI T'CCGG_A Cuts at: 6015 6015 Size: 6352 BspHI T'CATG_A Cuts at: 4241 5971 4241 Size: 1730 4622 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2331 2364 2697 2988 3157 3263 3264 3707 Size: 1487 33 333 291 169 106 1 443 Cuts at: 3707 844 Size: 3489 Fragments arranged by size: 3489 1487 443 333 291 169 106 33 1 BsrBI CCG'CTC Cuts at: 1394 1823 2569 4239 1394 Size: 429 746 1670 3507 Fragments arranged by size: 3507 1670 746 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4847 5021 65 Size: 1183 3599 174 1396 Fragments arranged by size: 3599 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3059 3122 4996 5183 1700 Size: 110 1249 63 1874 187 2869 Fragments arranged by size: 2869 1874 1249 187 110 63 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6352 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 3215 4412 5424 3215 Size: 1197 1012 4143 Fragments arranged by size: 4143 1197 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2365 3226 794 Size: 1571 861 3920 Fragments arranged by size: 3920 1571 861 BstBI TT'CG_AA Cuts at: 5156 5156 Size: 6352 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6352 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2816 2011 Size: 805 5547 BstYI r'GATC_y Cuts at: 1972 2172 2340 3682 4049 4434 4451 5903 5989 Size: 200 168 1342 367 385 17 1452 86 Cuts at: 5989 6001 6347 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1342 385 367 346 200 168 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2408 2803 3051 2408 Size: 395 248 5709 Fragments arranged by size: 5709 395 248 BtgI C'CryG_G Cuts at: 513 1065 2257 2442 3339 513 Size: 552 1192 185 897 3526 Fragments arranged by size: 3526 1192 897 552 185 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2208 487 Size: 683 56 982 4631 Fragments arranged by size: 4631 982 683 56 BtsI GCAGTG_nn' Cuts at: 2380 3182 3868 4662 4682 2380 Size: 802 686 794 20 4050 Fragments arranged by size: 4050 802 794 686 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6352 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6317 DraI TTT'AAA Cuts at: 3656 3656 Size: 6352 DraIII CAC_nnn'GTG Cuts at: 2557 2557 Size: 6352 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5359 809 Size: 507 4043 1802 Fragments arranged by size: 4043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 2818 2974 Size: 54 1425 50 275 18 333 655 156 Cuts at: 2974 3004 3082 3124 3298 3799 4688 6317 8 Size: 30 78 42 174 501 889 1629 43 Fragments arranged by size: 1629 1425 889 655 501 333 275 174 156 78 54 50 43 42 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 3799 1537 Size: 275 1312 675 4090 Fragments arranged by size: 4090 1312 675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2528 4282 1353 Size: 1175 1754 3423 Fragments arranged by size: 3423 1754 1175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4939 5313 5459 387 Size: 1064 138 3350 374 146 1280 Fragments arranged by size: 3350 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2436 2436 Size: 6352 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2114 2207 2675 2858 2931 3169 Size: 393 434 48 93 468 183 73 238 Cuts at: 3169 3224 3453 3479 4415 5012 5799 6067 1239 Size: 55 229 26 936 597 787 268 1524 Fragments arranged by size: 1524 936 787 597 468 434 393 268 238 229 183 93 73 55 48 26 EcoNI CCTnn'n_nnAGG Cuts at: 2960 3266 2960 Size: 306 6046 EcoO109I rG'GnC_Cy Cuts at: 2503 2520 2881 2965 3024 3238 2503 Size: 17 361 84 59 214 5617 Fragments arranged by size: 5617 361 214 84 59 17 EcoRI G'AATT_C Cuts at: 3661 4039 3661 Size: 378 5974 FseI GG_CCGG'CC Cuts at: 3063 3126 3063 Size: 63 6289 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 3203 3212 3360 3571 5499 1678 Size: 1525 9 148 211 1928 2531 Fragments arranged by size: 2531 1928 1525 211 148 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2740 2772 2870 2902 4993 Size: 32 135 32 1168 32 98 32 2091 Cuts at: 4993 5025 5067 5099 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2091 1168 135 98 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2272 3696 3942 669 Size: 988 281 334 1424 246 3079 Fragments arranged by size: 3079 1424 988 334 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6352 HpaI GTT'AAC Cuts at: 3942 3942 Size: 6352 KasI G'GCGC_C Cuts at: 3567 3567 Size: 6352 KpnI G_GTAC'C Cuts at: 3677 3677 Size: 6352 MfeI C'AATT_G Cuts at: 3951 3951 Size: 6352 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5466 5650 1305 Size: 4161 184 2007 Fragments arranged by size: 4161 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2820 2976 3006 3300 10 Size: 54 1425 343 988 156 30 294 3062 Fragments arranged by size: 3062 1425 988 343 294 156 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 4310 4669 4828 518 Size: 648 3144 359 159 2042 Fragments arranged by size: 3144 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2362 2452 2494 3499 3524 3768 4445 5593 Size: 756 90 42 1005 25 244 677 1148 Cuts at: 5593 5838 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1005 756 677 245 244 90 42 25 NaeI GCC'GGC Cuts at: 3061 3124 3061 Size: 63 6289 NarI GG'CG_CC Cuts at: 3568 3568 Size: 6352 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5800 NdeI CA'TA_TG Cuts at: 387 387 Size: 6352 NgoMIV G'CCGG_C Cuts at: 3059 3122 3059 Size: 63 6289 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6352 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 2663 3710 5255 1132 Size: 97 1434 1047 1545 2229 Fragments arranged by size: 2229 1545 1434 1047 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6352 PciI A'CATG_T Cuts at: 5251 5251 Size: 6352 PflMI CCAn_nnn'nTGG Cuts at: 1108 3633 1108 Size: 2525 3827 PfoI T'CCnGG_A Cuts at: 2499 3261 2499 Size: 762 5590 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PmlI CAC'GTG Cuts at: 2086 2086 Size: 6352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4421 4453 5934 5966 4421 Size: 32 1481 32 4807 Fragments arranged by size: 4807 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2503 2520 3238 2503 Size: 17 718 5617 Fragments arranged by size: 5617 718 17 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6352 PsiI TTA'TAA Cuts at: 3922 3922 Size: 6352 PspOMI G'GGCC_C Cuts at: 3023 3023 Size: 6352 PstI C_TGCA'G Cuts at: 830 2361 2465 3552 3704 830 Size: 1531 104 1087 152 3478 Fragments arranged by size: 3478 1531 1087 152 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 PvuII CAG'CTG Cuts at: 2452 2494 3499 2452 Size: 42 1005 5305 Fragments arranged by size: 5305 1005 42 SacI G_AGCT'C Cuts at: 2438 2438 Size: 6352 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SapI GCTCTTCn'nnn_ Cuts at: 2528 2528 Size: 6352 SbfI CC_TGCA'GG Cuts at: 3704 3704 Size: 6352 ScaI AGT'ACT Cuts at: 4600 6312 4600 Size: 1712 4640 SfcI C'TryA_G Cuts at: 826 1045 2357 2461 3352 3548 3700 4835 5516 Size: 219 1312 104 891 196 152 1135 681 Cuts at: 5516 5707 826 Size: 191 1471 Fragments arranged by size: 1471 1312 1135 891 681 219 196 191 152 104 SfoI GGC'GCC Cuts at: 3569 3569 Size: 6352 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 3250 3679 1872 Size: 1378 429 4545 Fragments arranged by size: 4545 1378 429 SmlI C'TyrA_G Cuts at: 820 1017 1943 3602 4456 5357 5619 820 Size: 197 926 1659 854 901 262 1553 Fragments arranged by size: 1659 1553 926 901 854 262 197 SnaBI TAC'GTA Cuts at: 493 4143 493 Size: 3650 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 3710 1132 Size: 2578 3774 SspI AAT'ATT Cuts at: 5 52 4276 5 Size: 47 4224 2081 Fragments arranged by size: 4224 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6352 StyI C'CwwG_G Cuts at: 513 1065 2846 3791 513 Size: 552 1781 945 3074 Fragments arranged by size: 3074 1781 945 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3359 3503 4379 4396 4549 4734 5179 Size: 1255 1779 144 876 17 153 185 445 Cuts at: 5179 325 Size: 1498 Fragments arranged by size: 1779 1498 1255 876 445 185 153 144 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4598 6310 96 Size: 274 80 33 51 1443 2621 1712 138 Fragments arranged by size: 2621 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2577 2609 2678 2710 3031 3063 3308 3340 6312 Size: 32 69 32 321 32 245 32 2972 Cuts at: 6312 6344 2577 Size: 32 2585 Fragments arranged by size: 2972 2585 321 245 69 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6352 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2041 2234 2551 2011 Size: 30 193 317 5812 Fragments arranged by size: 5812 317 193 30 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6352 XmaI C'CCGG_G Cuts at: 1870 3248 3677 1870 Size: 1378 429 4545 Fragments arranged by size: 4545 1378 429 XmnI GAAnn'nnTTC Cuts at: 4481 4481 Size: 6352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4160 276 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI