(Linear) (Six-base) MAPSORT of: KIEE0978.seq Check: 6339 from: 1 to: 1647 KIEE0978.orf With 182 enzymes: SgfI * January 29, 2014 11:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 359 692 983 1152 1647 Size: 359 333 291 169 495 Fragments arranged by size: 495 359 333 291 169 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 926 1448 1647 Size: 926 522 199 Fragments arranged by size: 926 522 199 AfeI AGC'GCT Cuts at: 0 1196 1647 Size: 1196 451 AflIII A'CryG_T Cuts at: 0 80 261 1647 Size: 80 181 1386 Fragments arranged by size: 1386 181 80 AlwNI CAG_nnn'CTG Cuts at: 0 360 1015 1647 Size: 360 655 632 Fragments arranged by size: 655 632 360 ApaI G_GGCC'C Cuts at: 0 1022 1647 Size: 1022 625 AvaI C'yCGr_G Cuts at: 0 1025 1243 1498 1647 Size: 1025 218 255 149 Fragments arranged by size: 1025 255 218 149 AvrII C'CTAG_G Cuts at: 0 841 1647 Size: 841 806 BanI G'GyrC_C Cuts at: 0 542 1562 1647 Size: 542 1020 85 Fragments arranged by size: 1020 542 85 BanII G_rGCy'C Cuts at: 0 433 719 1022 1034 1316 1647 Size: 433 286 303 12 282 331 Fragments arranged by size: 433 331 303 286 282 12 BbeI G_GCGC'C Cuts at: 0 1566 1647 Size: 1566 81 BbvCI CC'TCA_GC Cuts at: 0 448 1647 Size: 448 1199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 447 481 1647 Size: 447 34 1166 Fragments arranged by size: 1166 447 34 BglI GCCn_nnn'nGGC Cuts at: 0 1503 1560 1647 Size: 1503 57 87 Fragments arranged by size: 1503 87 57 BglII A'GATC_T Cuts at: 0 335 1647 Size: 335 1312 BlpI GC'TnA_GC Cuts at: 0 364 427 718 752 1647 Size: 364 63 291 34 895 Fragments arranged by size: 895 364 291 63 34 Bme1580I G_kGCm'C Cuts at: 0 1022 1647 Size: 1022 625 BmgBI CAC'GTC Cuts at: 0 243 286 1647 Size: 243 43 1361 Fragments arranged by size: 1361 243 43 BmrI ACTGGGnnnn_n' Cuts at: 0 1311 1647 Size: 1311 336 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 72 688 1647 Size: 72 616 959 Fragments arranged by size: 959 616 72 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 109 202 670 853 1164 1219 1474 Size: 61 48 93 468 183 311 55 255 Cuts at: 1474 1647 Size: 173 Fragments arranged by size: 468 311 255 183 173 93 61 55 48 Bpu10I CC'TnA_GC Cuts at: 0 47 448 610 1647 Size: 47 401 162 1037 Fragments arranged by size: 1037 401 162 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1582 1647 Size: 1582 65 BsaI GGTCTCn'nnnn_ Cuts at: 0 1418 1647 Size: 1418 229 BsaAI yAC'GTr Cuts at: 0 81 1647 Size: 81 1566 BsaHI Gr'CG_yC Cuts at: 0 1563 1647 Size: 1563 84 BsaWI w'CCGG_w Cuts at: 0 508 1647 Size: 508 1139 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 673 703 1303 1333 1647 Size: 673 30 600 30 314 Fragments arranged by size: 673 600 314 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 346 933 1171 1647 Size: 346 587 238 476 Fragments arranged by size: 587 476 346 238 BseYI C'CCAG_C Cuts at: 0 28 382 783 868 1015 1484 1515 1647 Size: 28 354 401 85 147 469 31 132 Fragments arranged by size: 469 401 354 147 132 85 31 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 505 628 961 1192 1647 Size: 505 123 333 231 455 Fragments arranged by size: 505 455 333 231 123 BsiEI CG_ry'CG Cuts at: 0 1122 1647 Size: 1122 525 BsiHKAI G_wGCw'C Cuts at: 0 433 1647 Size: 433 1214 Bsp1286I G_dGCh'C Cuts at: 0 433 719 1022 1034 1316 1647 Size: 433 286 303 12 282 331 Fragments arranged by size: 433 331 303 286 282 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 326 359 692 983 1152 1258 1259 1647 Size: 326 33 333 291 169 106 1 388 Fragments arranged by size: 388 333 326 291 169 106 33 1 BsrBI CCG'CTC Cuts at: 0 564 1647 Size: 564 1083 BsrFI r'CCGG_y Cuts at: 0 1054 1117 1647 Size: 1054 63 530 Fragments arranged by size: 1054 530 63 BssSI C'ACGA_G Cuts at: 0 1210 1647 Size: 1210 437 BstAPI GCAn_nnn'nTGC Cuts at: 0 360 1221 1647 Size: 360 861 426 Fragments arranged by size: 861 426 360 BstXI CCAn_nnnn'nTGG Cuts at: 0 811 1647 Size: 811 836 BstYI r'GATC_y Cuts at: 0 167 335 1647 Size: 167 168 1312 Fragments arranged by size: 1312 168 167 Bsu36I CC'TnA_GG Cuts at: 0 403 798 1046 1647 Size: 403 395 248 601 Fragments arranged by size: 601 403 395 248 BtgI C'CryG_G Cuts at: 0 252 437 1334 1647 Size: 252 185 897 313 Fragments arranged by size: 897 313 252 185 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 203 1647 Size: 203 1444 BtsI GCAGTG_nn' Cuts at: 0 375 1177 1647 Size: 375 802 470 Fragments arranged by size: 802 470 375 DraIII CAC_nnn'GTG Cuts at: 0 552 1647 Size: 552 1095 EaeI y'GGCC_r Cuts at: 0 158 813 969 999 1077 1119 1293 1647 Size: 158 655 156 30 78 42 174 354 Fragments arranged by size: 655 354 174 158 156 78 42 30 EagI C'GGCC_G Cuts at: 0 1119 1647 Size: 1119 528 EarI CTCTTCn'nnn_ Cuts at: 0 523 1647 Size: 523 1124 EcoICRI GAG'CTC Cuts at: 0 431 1647 Size: 431 1216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 109 202 670 853 926 1164 1219 Size: 61 48 93 468 183 73 238 55 Cuts at: 1219 1448 1474 1647 Size: 229 26 173 Fragments arranged by size: 468 238 229 183 173 93 73 61 55 48 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 955 1261 1647 Size: 955 306 386 Fragments arranged by size: 955 386 306 EcoO109I rG'GnC_Cy Cuts at: 0 498 515 876 960 1019 1233 1647 Size: 498 17 361 84 59 214 414 Fragments arranged by size: 498 414 361 214 84 59 17 FseI GG_CCGG'CC Cuts at: 0 1058 1121 1647 Size: 1058 63 526 Fragments arranged by size: 1058 526 63 HaeII r_GCGC'y Cuts at: 0 1198 1207 1355 1566 1647 Size: 1198 9 148 211 81 Fragments arranged by size: 1198 211 148 81 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 735 767 865 897 1647 Size: 735 32 98 32 750 Fragments arranged by size: 750 735 98 32 32 HincII GTy'rAC Cuts at: 0 267 1647 Size: 267 1380 KasI G'GCGC_C Cuts at: 0 1562 1647 Size: 1562 85 MscI TGG'CCA Cuts at: 0 815 971 1001 1295 1647 Size: 815 156 30 294 352 Fragments arranged by size: 815 352 294 156 30 MspA1I CmG'CkG Cuts at: 0 357 447 489 1494 1519 1647 Size: 357 90 42 1005 25 128 Fragments arranged by size: 1005 357 128 90 42 25 NaeI GCC'GGC Cuts at: 0 1056 1119 1647 Size: 1056 63 528 Fragments arranged by size: 1056 528 63 NarI GG'CG_CC Cuts at: 0 1563 1647 Size: 1563 84 NgoMIV G'CCGG_C Cuts at: 0 1054 1117 1647 Size: 1054 63 530 Fragments arranged by size: 1054 530 63 NspI r_CATG'y Cuts at: 0 658 1647 Size: 658 989 PflMI CCAn_nnn'nTGG Cuts at: 0 1628 1647 Size: 1628 19 PfoI T'CCnGG_A Cuts at: 0 494 1256 1647 Size: 494 762 391 Fragments arranged by size: 762 494 391 PmlI CAC'GTG Cuts at: 0 81 1647 Size: 81 1566 PpuMI rG'GwC_Cy Cuts at: 0 498 515 1233 1647 Size: 498 17 718 414 Fragments arranged by size: 718 498 414 17 PspOMI G'GGCC_C Cuts at: 0 1018 1647 Size: 1018 629 PstI C_TGCA'G Cuts at: 0 356 460 1547 1647 Size: 356 104 1087 100 Fragments arranged by size: 1087 356 104 100 PvuII CAG'CTG Cuts at: 0 447 489 1494 1647 Size: 447 42 1005 153 Fragments arranged by size: 1005 447 153 42 SacI G_AGCT'C Cuts at: 0 433 1647 Size: 433 1214 SapI GCTCTTCn'nnn_ Cuts at: 0 523 1647 Size: 523 1124 SfcI C'TryA_G Cuts at: 0 352 456 1347 1543 1647 Size: 352 104 891 196 104 Fragments arranged by size: 891 352 196 104 104 SfoI GGC'GCC Cuts at: 0 1564 1647 Size: 1564 83 SmaI CCC'GGG Cuts at: 0 1245 1647 Size: 1245 402 SmlI C'TyrA_G Cuts at: 0 1597 1647 Size: 1597 50 StyI C'CwwG_G Cuts at: 0 841 1647 Size: 841 806 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1354 1498 1647 Size: 1354 144 149 Fragments arranged by size: 1354 149 144 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 572 604 673 705 1026 1058 1303 1335 Size: 572 32 69 32 321 32 245 32 Cuts at: 1335 1647 Size: 312 Fragments arranged by size: 572 321 312 245 69 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 36 229 546 1647 Size: 36 193 317 1101 Fragments arranged by size: 1101 317 193 36 XmaI C'CCGG_G Cuts at: 0 1243 1647 Size: 1243 404 Enzymes that do cut: AarI AcuI AfeI AflIII AlwNI ApaI AvaI AvrII BanI BanII BbeI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII KasI MscI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SapI SfcI SfoI SmaI SmlI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbsI BciVI BclI BfrBI BmtI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EciI EcoRI EcoRV FalI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI