################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BcgI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BseRI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HincII HindII HindIII HinfI HpaI KpnI MaeI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 406 GGCGCC G[GA]^CG[CT]C Alw44I 311 GTGCAC G^TGCAC ApaI 762 GGGCCC GGGCC^C ApaLI 311 GTGCAC G^TGCAC AspI 349 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 13 GGATCC G^GATCC BbeI 406 GGCGCC GGCGC^C BbvCI 135 CCTCAGC CCTCAGC(-5/-2) BclI 365 TGATCA T^GATCA BsaHI 406 GGCGCC G[GA]^CG[CT]C BsaWI 197 ACCGGA [AT]^CCGG[AT] BsiEI 284 CGGCCG CG[GA][CT]^CG BsmAI 280 GAGAC GTCTC(1/5) BsmBI 280 GAGACG CGTCTC(1/5) BsrBI 261 GAGCGG CCGCTC(-3/-3) BsrDI 866 GCAATG GCAATG(2/0) BsrFI 307 GCCGGT [GA]^CCGG[CT] BssSI 574 CTCGTG CACGAG(-5/-1) BstYI 13 GGATCC [GA]^GATC[CT] Cfr10I 307 GCCGGT [GA]^CCGG[CT] DdeI 136 CTCAG C^T[ACGT]AG EagI 284 CGGCCG C^GGCCG EclXI 284 CGGCCG C^GGCCG Eco52I 284 CGGCCG C^GGCCG EcoNI 297 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 365 TGATCA T^GATCA HgaI 921 GACGC GACGC(5/10) Hin1I 406 GGCGCC G[GA]^CG[CT]C KasI 406 GGCGCC G^GCGCC KspI 475 CCGCGG CCGC^GG MflI 13 GGATCC [GA]^GATC[CT] MseI 710 TTAA T^TAA NarI 406 GGCGCC GG^CGCC PflFI 349 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 624 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 282 GACGGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 910 GAGCTC GAGCT^C SacII 475 CCGCGG CCGC^GG SfoI 406 GGCGCC GGC^GCC SmlI 925 CTCAAG C^T[CT][GA]AG TaqI 878 TCGA T^CGA Tru9I 710 TTAA T^TAA Tth111I 349 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 878 TCGA T^CGA Van91I 624 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 13 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AfaI 219 GTAC GT^AC 618 GTAC AlwI 14 GATCC GGATC(4/5) 13 GGATC AlwNI 340 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 390 CAGCCTCTG BalI 179 TGGCCA TGG^CCA 509 TGGCCA BanI 101 GGTGCC G^G[CT][GA]CC 406 GGCGCC BbrPI 553 CACGTG CAC^GTG 813 CACGTG BglI 490 GCCGTTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 707 GCCTTAAAGGC BsaAI 553 CACGTG [CT]AC^GT[GA] 813 CACGTG BsgI 205 CTGCAC GTGCAG(16/14) 7 GTGCAG BsrI 315 ACTGG ACTGG(1/-1) 512 CCAGT DpnII 14 GATC ^GATC 366 GATC DraII 761 GGGGCCC [GA]G^G[ACGT]CC[CT] 762 GGGCCCC DsaI 416 CCGTGG C^C[GA][CT]GG 475 CCGCGG EarI 130 CTCTTC CTCTTC(1/4) 532 CTCTTC EcoO109I 761 GGGGCCC [GA]G^G[ACGT]CC[CT] 762 GGGCCCC FauI 263 GCGGG CCCGC(4/6) 477 GCGGG Ksp632I 130 CTCTTC CTCTTC(1/4) 532 CTCTTC MaeIII 466 GTGAC ^GT[ACGT]AC 721 GTCAC MboI 14 GATC ^GATC 366 GATC MluNI 179 TGGCCA TGG^CCA 509 TGGCCA MscI 179 TGGCCA TGG^CCA 509 TGGCCA NdeII 14 GATC ^GATC 366 GATC NspI 626 ACATGC [GA]CATG^[CT] 940 ACATGC PmaCI 553 CACGTG CAC^GTG 813 CACGTG PmlI 553 CACGTG CAC^GTG 813 CACGTG PstI 337 CTGCAG CTGCA^G 372 CTGCAG PvuII 67 CAGCTG CAG^CTG 343 CAGCTG RsaI 219 GTAC GT^AC 618 GTAC SapI 129 GCTCTTC GCTCTTC(1/4) 531 GCTCTTC Sau3AI 14 GATC ^GATC 366 GATC SfcI 337 CTGCAG C^T[GA][CT]AG 372 CTGCAG SmaI 323 CCCGGG CCC^GGG 635 CCCGGG Tsp45I 466 GTGAC ^GT[GC]AC 721 GTCAC XcmI 59 CCATCCCCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 693 CCACCAGGGCTTTGG XmaCI 323 CCCGGG C^CCGGG 635 CCCGGG XmaI 323 CCCGGG C^CCGGG 635 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AluI AspHI AvaI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I EaeI EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TseI TspRI