(Circular) (Six-base) MAPSORT of: pFN21AE9521.seq Check: 1088 from: 1 to: 5656 pFN21AE9521 5656 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2384 2384 Size: 5656 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2512 3719 5103 5371 1632 Size: 880 1207 1384 268 1917 Fragments arranged by size: 1917 1384 1207 880 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 4555 5532 1203 Size: 3352 977 1327 Fragments arranged by size: 3352 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2402 1184 Size: 1218 4438 AlwNI CAG_nnn'CTG Cuts at: 2350 2400 3399 4971 2350 Size: 50 999 1572 3035 Fragments arranged by size: 3035 1572 999 50 ApaI G_GGCC'C Cuts at: 2771 2771 Size: 5656 ApaLI G'TGCA_C Cuts at: 2316 3713 4869 2316 Size: 1397 1156 3103 Fragments arranged by size: 3103 1397 1156 ApoI r'AATT_y Cuts at: 1493 2965 3191 3343 5452 1493 Size: 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2328 2640 2807 2981 1870 Size: 73 385 312 167 174 4545 Fragments arranged by size: 4545 385 312 174 167 73 BamHI G'GATC_C Cuts at: 2018 2986 3353 2018 Size: 968 367 4321 Fragments arranged by size: 4321 968 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2106 2411 2977 4432 618 Size: 325 217 627 319 305 566 1455 1842 Fragments arranged by size: 1842 1455 627 566 325 319 305 217 BanII G_rGCy'C Cuts at: 1391 2042 2104 2136 2771 2919 2975 1391 Size: 651 62 32 635 148 56 4072 Fragments arranged by size: 4072 651 635 148 62 56 32 BbeI G_GCGC'C Cuts at: 2415 2415 Size: 5656 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2035 2518 2578 928 Size: 971 136 483 60 4006 Fragments arranged by size: 4006 971 483 136 60 BbvCI CC'TCA_GC Cuts at: 2141 2141 Size: 5656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 2370 1548 Size: 822 4834 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2501 2718 4267 Size: 107 122 71 631 605 829 217 1549 Cuts at: 4267 136 Size: 1525 Fragments arranged by size: 1549 1525 829 631 605 217 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 2320 2771 3717 4873 1790 Size: 530 451 946 1156 2573 Fragments arranged by size: 2573 1156 946 530 451 BmgBI CAC'GTC Cuts at: 1318 2368 1318 Size: 1050 4606 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2303 2327 4316 1239 Size: 893 171 24 1989 2579 Fragments arranged by size: 2579 1989 893 171 24 Bpu10I CC'TnA_GC Cuts at: 2141 2141 Size: 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2915 3781 4646 4944 2915 Size: 866 865 298 3627 Fragments arranged by size: 3627 866 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4319 882 Size: 617 2820 2219 Fragments arranged by size: 2820 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2560 2820 3447 493 Size: 711 1356 260 627 2702 Fragments arranged by size: 2702 1356 711 627 260 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2412 3463 3845 4480 Size: 53 83 186 936 879 1051 382 635 Cuts at: 4480 275 Size: 1451 Fragments arranged by size: 1451 1051 936 879 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2202 4089 4487 4761 4908 5319 2202 Size: 1887 398 274 147 411 2539 Fragments arranged by size: 2539 1887 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5431 Fragments arranged by size: 5431 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5656 BseYI C'CCAG_C Cuts at: 2070 2074 2743 2763 4859 5355 2070 Size: 4 669 20 2096 496 2371 Fragments arranged by size: 2371 2096 669 496 20 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 2195 1163 Size: 573 297 162 4624 Fragments arranged by size: 4624 573 297 162 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2292 3106 3867 4895 1540 Size: 275 186 291 814 761 1028 2301 Fragments arranged by size: 2301 1028 814 761 291 275 186 BsiHKAI G_wGCw'C Cuts at: 2320 2610 2814 2919 3717 3802 4873 2320 Size: 290 204 105 798 85 1071 3103 Fragments arranged by size: 3103 1071 798 290 204 105 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 BsmBI CGTCTCn'nnnn_ Cuts at: 2279 2279 Size: 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2104 2136 2320 2610 2771 2814 Size: 399 252 62 32 184 290 161 43 Cuts at: 2814 2919 2975 3717 3802 4873 1391 Size: 105 56 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 290 252 184 161 105 85 62 56 43 32 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 2265 2384 3011 844 Size: 1373 48 119 627 3489 Fragments arranged by size: 3489 1373 627 119 48 BsrBI CCG'CTC Cuts at: 1394 1823 2268 3543 1394 Size: 429 445 1275 3507 Fragments arranged by size: 3507 1275 445 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2878 4151 4325 65 Size: 1183 1630 1273 174 1396 Fragments arranged by size: 1630 1396 1273 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2312 4300 4487 1700 Size: 110 502 1988 187 2869 Fragments arranged by size: 2869 1988 502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 2579 3716 4728 2579 Size: 1137 1012 3507 Fragments arranged by size: 3507 1137 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstXI CCAn_nnnn'nTGG Cuts at: 2422 2512 2708 2743 2422 Size: 90 196 35 5335 Fragments arranged by size: 5335 196 90 35 BstYI r'GATC_y Cuts at: 1972 2018 2986 3353 3738 3755 5207 5293 5305 Size: 46 968 367 385 17 1452 86 12 Cuts at: 5305 5651 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 968 385 367 346 86 46 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2421 2480 513 Size: 552 939 417 59 3689 Fragments arranged by size: 3689 939 552 417 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2176 2401 2527 2650 487 Size: 683 56 950 225 126 123 3493 Fragments arranged by size: 3493 950 683 225 126 123 56 BtsI GCAGTG_nn' Cuts at: 2450 3172 3966 3986 2450 Size: 722 794 20 4120 Fragments arranged by size: 4120 794 722 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2289 2502 Size: 54 1425 50 275 18 354 105 213 Cuts at: 2502 2514 2562 2613 3103 3992 5621 8 Size: 12 48 51 490 889 1629 43 Fragments arranged by size: 1629 1425 889 490 354 275 213 105 54 51 50 48 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2289 3103 1537 Size: 275 477 814 4090 Fragments arranged by size: 4090 814 477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2141 2543 3586 1353 Size: 788 402 1043 3423 Fragments arranged by size: 3423 1043 788 402 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2917 2917 Size: 5656 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2303 2327 2512 3719 4316 5103 Size: 393 500 171 24 185 1207 597 787 Cuts at: 5103 5371 1239 Size: 268 1524 Fragments arranged by size: 1524 1207 787 597 500 393 268 185 171 24 EcoNI CCTnn'n_nnAGG Cuts at: 2306 2306 Size: 5656 EcoO109I rG'GnC_Cy Cuts at: 2767 2768 2767 Size: 1 5655 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 2415 2692 2724 4803 1678 Size: 737 277 32 2079 2531 Fragments arranged by size: 2531 2079 737 277 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373 Size: 32 135 32 2725 32 42 32 2626 Fragments arranged by size: 2725 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KasI G'GCGC_C Cuts at: 2411 2411 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 4770 4954 1305 Size: 927 2538 184 2007 Fragments arranged by size: 2538 2007 927 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 2516 10 Size: 54 1425 343 354 330 3150 Fragments arranged by size: 3150 1425 354 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2366 2420 2510 2615 3614 3973 Size: 648 843 357 54 90 105 999 359 Cuts at: 3973 4132 518 Size: 159 2042 Fragments arranged by size: 2042 999 843 648 359 357 159 105 90 54 MspA1I CmG'CkG Cuts at: 1606 2074 2350 2482 3072 3749 4897 5142 1606 Size: 468 276 132 590 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 590 468 276 245 132 NarI GG'CG_CC Cuts at: 2412 2412 Size: 5656 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4165 Fragments arranged by size: 4165 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2635 2949 3014 4559 1132 Size: 97 1406 314 65 1545 2229 Fragments arranged by size: 2229 1545 1406 314 97 65 PasI CC'CwG_GG Cuts at: 1348 2659 1348 Size: 1311 4345 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 2635 1108 Size: 1527 4129 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PmlI CAC'GTG Cuts at: 2560 2820 2560 Size: 260 5396 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2198 2230 3725 3757 5238 5270 2198 Size: 32 1495 32 1481 32 2584 Fragments arranged by size: 2584 1495 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2291 1613 Size: 678 4978 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PspOMI G'GGCC_C Cuts at: 2767 2767 Size: 5656 PstI C_TGCA'G Cuts at: 830 2346 2381 3008 830 Size: 1516 35 627 3478 Fragments arranged by size: 3478 1516 627 35 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2074 2350 2074 Size: 276 5380 SacI G_AGCT'C Cuts at: 2919 2919 Size: 5656 SacII CC_GC'GG Cuts at: 2483 2483 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SapI GCTCTTCn'nnn_ Cuts at: 2141 2543 2141 Size: 402 5254 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2342 2377 3004 4139 4820 5011 826 Size: 219 1297 35 627 1135 681 191 1471 Fragments arranged by size: 1471 1297 1135 681 627 219 191 35 SfoI GGC'GCC Cuts at: 2413 2413 Size: 5656 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2330 2642 2983 1872 Size: 458 312 341 4545 Fragments arranged by size: 4545 458 341 312 SmlI C'TyrA_G Cuts at: 820 1017 1943 2930 3760 4661 4923 820 Size: 197 926 987 830 901 262 1553 Fragments arranged by size: 1553 987 926 901 830 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SrfI GCCC'GGGC Cuts at: 2330 2642 2330 Size: 312 5344 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2004 3095 513 Size: 552 939 1091 3074 Fragments arranged by size: 3074 1091 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2233 2500 3683 3700 3853 4038 4483 Size: 1255 653 267 1183 17 153 185 445 Cuts at: 4483 325 Size: 1498 Fragments arranged by size: 1498 1255 1183 653 445 267 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2223 3902 5614 Size: 274 80 33 51 1443 246 1679 1712 Cuts at: 5614 96 Size: 138 Fragments arranged by size: 1712 1679 1443 274 246 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2781 2813 5616 5648 2781 Size: 32 2803 32 2789 Fragments arranged by size: 2803 2789 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2357 1316 Size: 1041 4615 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2071 2705 2071 Size: 634 5022 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2328 2640 2981 1870 Size: 458 312 341 4545 Fragments arranged by size: 4545 458 341 312 XmnI GAAnn'nnTTC Cuts at: 3785 3785 Size: 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SwaI