(Linear) (Six-base) MAPSORT of: KIEE9521.seq Check: 1507 from: 1 to: 951 KIEE9521.orf With 182 enzymes: SgfI * January 29, 2014 12:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 379 951 Size: 379 572 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 507 951 Size: 507 444 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 397 951 Size: 397 554 AlwNI CAG_nnn'CTG Cuts at: 0 345 395 951 Size: 345 50 556 Fragments arranged by size: 556 345 50 ApaI G_GGCC'C Cuts at: 0 766 951 Size: 766 185 ApaLI G'TGCA_C Cuts at: 0 311 951 Size: 311 640 AvaI C'yCGr_G Cuts at: 0 323 635 802 951 Size: 323 312 167 149 Fragments arranged by size: 323 312 167 149 BamHI G'GATC_C Cuts at: 0 13 951 Size: 13 938 BanI G'GyrC_C Cuts at: 0 101 406 951 Size: 101 305 545 Fragments arranged by size: 545 305 101 BanII G_rGCy'C Cuts at: 0 37 99 131 766 914 951 Size: 37 62 32 635 148 37 Fragments arranged by size: 635 148 62 37 37 32 BbeI G_GCGC'C Cuts at: 0 410 951 Size: 410 541 BbsI GAAGACnn'nnnn_ Cuts at: 0 30 513 573 951 Size: 30 483 60 378 Fragments arranged by size: 483 378 60 30 BbvCI CC'TCA_GC Cuts at: 0 136 951 Size: 136 815 BclI T'GATC_A Cuts at: 0 365 951 Size: 365 586 BglI GCCn_nnn'nGGC Cuts at: 0 496 713 951 Size: 496 217 238 Fragments arranged by size: 496 238 217 Bme1580I G_kGCm'C Cuts at: 0 315 766 951 Size: 315 451 185 Fragments arranged by size: 451 315 185 BmgBI CAC'GTC Cuts at: 0 363 951 Size: 363 588 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 298 322 951 Size: 127 171 24 629 Fragments arranged by size: 629 171 127 24 Bpu10I CC'TnA_GC Cuts at: 0 136 951 Size: 136 815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 910 951 Size: 910 41 BsaAI yAC'GTr Cuts at: 0 555 815 951 Size: 555 260 136 Fragments arranged by size: 555 260 136 BsaHI Gr'CG_yC Cuts at: 0 407 951 Size: 407 544 BsaWI w'CCGG_w Cuts at: 0 197 951 Size: 197 754 BseYI C'CCAG_C Cuts at: 0 65 69 738 758 951 Size: 65 4 669 20 193 Fragments arranged by size: 669 193 65 20 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 190 951 Size: 28 162 761 Fragments arranged by size: 761 162 28 BsiEI CG_ry'CG Cuts at: 0 287 951 Size: 287 664 BsiHKAI G_wGCw'C Cuts at: 0 315 605 809 914 951 Size: 315 290 204 105 37 Fragments arranged by size: 315 290 204 105 37 BsmBI CGTCTCn'nnnn_ Cuts at: 0 274 951 Size: 274 677 Bsp1286I G_dGCh'C Cuts at: 0 37 99 131 315 605 766 809 914 Size: 37 62 32 184 290 161 43 105 Cuts at: 914 951 Size: 37 Fragments arranged by size: 290 184 161 105 62 43 37 37 32 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 212 260 379 951 Size: 212 48 119 572 Fragments arranged by size: 572 212 119 48 BsrBI CCG'CTC Cuts at: 0 263 951 Size: 263 688 BsrDI GCAATG_nn' Cuts at: 0 873 951 Size: 873 78 BsrFI r'CCGG_y Cuts at: 0 307 951 Size: 307 644 BssSI C'ACGA_G Cuts at: 0 574 951 Size: 574 377 BstXI CCAn_nnnn'nTGG Cuts at: 0 417 507 703 738 951 Size: 417 90 196 35 213 Fragments arranged by size: 417 213 196 90 35 BstYI r'GATC_y Cuts at: 0 13 951 Size: 13 938 BtgI C'CryG_G Cuts at: 0 416 475 951 Size: 416 59 476 Fragments arranged by size: 476 416 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 171 396 522 645 951 Size: 171 225 126 123 306 Fragments arranged by size: 306 225 171 126 123 BtsI GCAGTG_nn' Cuts at: 0 445 951 Size: 445 506 EaeI y'GGCC_r Cuts at: 0 179 284 497 509 557 608 951 Size: 179 105 213 12 48 51 343 Fragments arranged by size: 343 213 179 105 51 48 12 EagI C'GGCC_G Cuts at: 0 284 951 Size: 284 667 EarI CTCTTCn'nnn_ Cuts at: 0 136 538 951 Size: 136 402 413 Fragments arranged by size: 413 402 136 EcoICRI GAG'CTC Cuts at: 0 912 951 Size: 912 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 298 322 507 951 Size: 127 171 24 185 444 Fragments arranged by size: 444 185 171 127 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 301 951 Size: 301 650 EcoO109I rG'GnC_Cy Cuts at: 0 762 763 951 Size: 762 1 188 Fragments arranged by size: 762 188 1 HaeII r_GCGC'y Cuts at: 0 410 687 719 951 Size: 410 277 32 232 Fragments arranged by size: 410 277 232 32 KasI G'GCGC_C Cuts at: 0 406 951 Size: 406 545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 227 951 Size: 227 724 MscI TGG'CCA Cuts at: 0 181 511 951 Size: 181 330 440 Fragments arranged by size: 440 330 181 MslI CAynn'nnrTG Cuts at: 0 361 415 505 610 951 Size: 361 54 90 105 341 Fragments arranged by size: 361 341 105 90 54 MspA1I CmG'CkG Cuts at: 0 69 345 477 951 Size: 69 276 132 474 Fragments arranged by size: 474 276 132 69 NarI GG'CG_CC Cuts at: 0 407 951 Size: 407 544 NspI r_CATG'y Cuts at: 0 630 944 951 Size: 630 314 7 Fragments arranged by size: 630 314 7 PasI CC'CwG_GG Cuts at: 0 654 951 Size: 654 297 PflMI CCAn_nnn'nTGG Cuts at: 0 630 951 Size: 630 321 PmlI CAC'GTG Cuts at: 0 555 815 951 Size: 555 260 136 Fragments arranged by size: 555 260 136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 193 225 951 Size: 193 32 726 Fragments arranged by size: 726 193 32 PshAI GACnn'nnGTC Cuts at: 0 286 951 Size: 286 665 PspOMI G'GGCC_C Cuts at: 0 762 951 Size: 762 189 PstI C_TGCA'G Cuts at: 0 341 376 951 Size: 341 35 575 Fragments arranged by size: 575 341 35 PvuII CAG'CTG Cuts at: 0 69 345 951 Size: 69 276 606 Fragments arranged by size: 606 276 69 SacI G_AGCT'C Cuts at: 0 914 951 Size: 914 37 SacII CC_GC'GG Cuts at: 0 478 951 Size: 478 473 SapI GCTCTTCn'nnn_ Cuts at: 0 136 538 951 Size: 136 402 413 Fragments arranged by size: 413 402 136 SfcI C'TryA_G Cuts at: 0 337 372 951 Size: 337 35 579 Fragments arranged by size: 579 337 35 SfoI GGC'GCC Cuts at: 0 408 951 Size: 408 543 SmaI CCC'GGG Cuts at: 0 325 637 951 Size: 325 312 314 Fragments arranged by size: 325 314 312 SmlI C'TyrA_G Cuts at: 0 925 951 Size: 925 26 SrfI GCCC'GGGC Cuts at: 0 325 637 951 Size: 325 312 314 Fragments arranged by size: 325 314 312 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 228 495 951 Size: 228 267 456 Fragments arranged by size: 456 267 228 TatI w'GTAC_w Cuts at: 0 218 951 Size: 218 733 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 776 808 951 Size: 776 32 143 Fragments arranged by size: 776 143 32 Tth111I GACn'n_nGTC Cuts at: 0 352 951 Size: 352 599 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 66 700 951 Size: 66 634 251 Fragments arranged by size: 634 251 66 XmaI C'CCGG_G Cuts at: 0 323 635 951 Size: 323 312 316 Fragments arranged by size: 323 316 312 Enzymes that do cut: AarI AcuI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI MmeI MscI MslI MspA1I NarI NspI PasI PflMI PmlI PpiI PshAI PspOMI PstI PvuII SacI SacII SapI SfcI SfoI SmaI SmlI SrfI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BglII BlpI BmrI BmtI BplI BsaI BsaBI BsaXI BseRI BsiWI BsmI BspEI BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PpuMI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI StuI StyI SwaI XbaI XhoI XmnI ZraI