################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccIII AflIII AgeI AhdI AocI Aor51HI AscI Asp718I AspEI AspI AvaI AvrII BbrPI BciVI BglI BglII BlnI BsaAI BsaI BseAI BsiEI BsiWI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FseI HindIII HpaI KpnI MluI MroI NaeI NdeI NgoMIV NheI NotI NruI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tsp45I Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AclI 1932 AACGTT AA^CGTT AcyI 130 GGCGCC G[GA]^CG[CT]C AfaI 1882 GTAC GT^AC Alw44I 1483 GTGCAC G^TGCAC AlwNI 2608 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1483 GTGCAC G^TGCAC Asp700I 1071 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1042 TGCGCA TGC^GCA BamHI 3 GGATCC G^GATCC BanI 130 GGCGCC G^G[CT][GA]CC BbeI 130 GGCGCC GGCGC^C BbsI 1612 GAAGAC GAAGAC(2/6) BbvCI 1853 CCTCAGC CCTCAGC(-5/-2) BbvI 2529 GCAGC GCAGC(8/12) BcgI 1869 CGAGAACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 206 TGATCA T^GATCA BcnI 126 CCGGG CC^[GC]GG BlpI 2605 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2605 GCTCAGC GC^T[ACGT]AGC BpuAI 1612 GAAGAC GAAGAC(2/6) BsaBI 564 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 130 GGCGCC G[GA]^CG[CT]C BsaWI 474 ACCGGA [AT]^CCGG[AT] BsmBI 1066 GAGACG CGTCTC(1/5) Bsp1407I 1881 TGTACA T^GTACA BsrDI 2038 GCAATG GCAATG(2/0) BsrGI 1881 TGTACA T^GTACA BstAPI 1045 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1964 TTCGAA TT^CGAA Cac8I 888 GCTGGC GC[ACGT]^[ACGT]GC CelII 2605 GCTCAGC GC^T[ACGT]AGC DraI 1326 TTTAAA TTT^AAA EcoNI 31 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1988 ATGCAT ATGCA^T FauI 13 GCGGG CCCGC(4/6) FbaI 206 TGATCA T^GATCA FspI 1042 TGCGCA TGC^GCA HaeII 130 GGCGCC [GA]GCGC^[CT] HgaI 1145 GACGC GACGC(5/10) Hin1I 130 GGCGCC G[GA]^CG[CT]C HphI 2398 GGTGA GGTGA(8/7) KasI 130 GGCGCC G^GCGCC KspI 11 CCGCGG CCGC^GG MamI 564 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 668 CAATTG C^AATTG MspA1I 11 CCGCGG C[AC]G^C[GT]G MunI 668 CAATTG C^AATTG NarI 130 GGCGCC GG^CGCC NciI 126 CCGGG CC^[GC]GG NsiI 1988 ATGCAT ATGCA^T NspV 1964 TTCGAA TT^CGAA Psp1406I 1932 AACGTT AA^CGTT RsaI 1882 GTAC GT^AC SacII 11 CCGCGG CCGC^GG SapI 1303 GAAGAGC GCTCTTC(1/4) SfoI 130 GGCGCC GGC^GCC SfuI 1964 TTCGAA TT^CGAA SspBI 1881 TGTACA T^GTACA TseI 2529 GCAGC G^C[AT]GC XbaI 429 TCTAGA T^CTAGA XmnI 1071 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 316 GTCTAC GT^[AC][GT]AC 2275 GTAGAC AccII 12 CGCG CG^CG 42 CGCG AflII 1471 CTTAAG C^TTAAG 1644 CTTAAG ApaI 1002 GGGCCC GGGCC^C 2258 GGGCCC AseI 556 ATTAAT AT^TAAT 1984 ATTAAT AsnI 556 ATTAAT AT^TAAT 1984 ATTAAT AspHI 1306 GAGCAC G[AT]GC[AT]^C 1483 GTGCAC BalI 890 TGGCCA TGG^CCA 2389 TGGCCA BanII 1002 GGGCCC G[GA]GC[CT]^C 2258 GGGCCC BfrI 1471 CTTAAG C^TTAAG 1644 CTTAAG BsgI 1462 GTGCAG GTGCAG(16/14) 2429 GTGCAG BsiHKAI 1306 GAGCAC G[AT]GC[AT]^C 1483 GTGCAC BsmAI 1066 GAGAC GTCTC(1/5) 357 GTCTC BstUI 12 CGCG CG^CG 42 CGCG EaeI 890 TGGCCA [CT]^GGCC[GA] 2389 TGGCCA HapII 126 CCGG C^CGG 475 CCGG HincII 1348 GTTGAC GT[CT]^[GA]AC 1493 GTCAAC HindII 1348 GTTGAC GT[CT]^[GA]AC 1493 GTCAAC HpaII 126 CCGG C^CGG 475 CCGG MaeII 1933 ACGT A^CGT 2490 ACGT MluNI 890 TGGCCA TGG^CCA 2389 TGGCCA MscI 890 TGGCCA TGG^CCA 2389 TGGCCA MspI 126 CCGG C^CGG 475 CCGG MvnI 12 CGCG CG^CG 42 CGCG NcoI 2075 CCATGG C^CATGG 2262 CCATGG PpuMI 1466 AGGACCT [GA]G^G[AT]CC[CT] 1908 AGGTCCT PshAI 1240 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1351 GACTCCAGTC PshBI 556 ATTAAT AT^TAAT 1984 ATTAAT PstI 298 CTGCAG CTGCA^G 2527 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstYI CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I XhoII