(Circular) (Six-base) MAPSORT of: pFN21AE3317.seq Check: 8589 from: 1 to: 7342 pFN21AE3317 7342 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5152 278 Size: 53 83 186 936 3616 2468 Fragments arranged by size: 3616 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2322 4281 4685 1937 Size: 385 1959 404 4594 Fragments arranged by size: 4594 1959 404 385 Acc65I G'GTAC_C Cuts at: 4663 4663 Size: 7342 AclI AA'CG_TT Cuts at: 1563 3938 5469 5842 1563 Size: 2375 1531 373 3063 Fragments arranged by size: 3063 2375 1531 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2439 3327 4492 5405 6789 7057 1632 Size: 807 888 1165 913 1384 268 1917 Fragments arranged by size: 1917 1384 1165 913 888 807 268 AflII C'TTAA_G Cuts at: 820 1017 3476 3649 820 Size: 197 2459 173 4513 Fragments arranged by size: 4513 2459 197 173 AflIII A'CryG_T Cuts at: 1203 6241 7218 1203 Size: 5038 977 1327 Fragments arranged by size: 5038 1327 977 AgeI A'CCGG_T Cuts at: 6173 6173 Size: 7342 AhdI GACnn_n'nnGTC Cuts at: 6071 6071 Size: 7342 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7342 AlwNI CAG_nnn'CTG Cuts at: 4618 5085 6657 4618 Size: 467 1572 5303 Fragments arranged by size: 5303 1572 467 ApaI G_GGCC'C Cuts at: 3011 4267 3011 Size: 1256 6086 ApaLI G'TGCA_C Cuts at: 3488 5399 6555 3488 Size: 1911 1156 4275 Fragments arranged by size: 4275 1911 1156 ApoI r'AATT_y Cuts at: 1493 2105 3340 3729 4215 4313 4326 4651 4877 Size: 612 1235 389 486 98 13 325 226 Cuts at: 4877 5029 7138 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1235 612 486 389 325 226 152 98 13 AseI AT'TA_AT Cuts at: 160 1032 2562 3990 5896 160 Size: 872 1530 1428 1906 1606 Fragments arranged by size: 1906 1606 1530 1428 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7342 AvaI C'yCGr_G Cuts at: 1870 1943 4667 1870 Size: 73 2724 4545 Fragments arranged by size: 4545 2724 73 BamHI G'GATC_C Cuts at: 2008 4672 5039 2008 Size: 2664 367 4311 Fragments arranged by size: 4311 2664 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2135 4663 6118 618 Size: 325 217 627 348 2528 1455 1842 Fragments arranged by size: 2528 1842 1455 627 348 325 217 BanII G_rGCy'C Cuts at: 1391 3011 4267 4661 1391 Size: 1620 1256 394 4072 Fragments arranged by size: 4072 1620 1256 394 BbeI G_GCGC'C Cuts at: 2139 2139 Size: 7342 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3624 928 Size: 971 1725 4646 Fragments arranged by size: 4646 1725 971 BbvCI CC'TCA_GC Cuts at: 3859 3859 Size: 7342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3863 3897 5533 5567 1903 Size: 34 1926 34 1636 34 3678 Fragments arranged by size: 3678 1926 1636 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5234 6444 5234 Size: 1210 6132 BclI T'GATC_A Cuts at: 1548 2211 1548 Size: 663 6679 BfrBI ATG'CAT Cuts at: 3995 3995 Size: 7342 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5953 136 Size: 107 122 71 631 605 4281 1525 Fragments arranged by size: 4281 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7337 7337 Size: 7342 BlpI GC'TnA_GC Cuts at: 4611 4759 4611 Size: 148 7194 Bme1580I G_kGCm'C Cuts at: 1790 3011 3136 3492 4267 4294 5403 6559 1790 Size: 1221 125 356 775 27 1109 1156 2573 Fragments arranged by size: 2573 1221 1156 1109 775 356 125 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7342 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4531 5073 6031 Size: 416 53 83 399 24 3114 542 958 Cuts at: 6031 442 Size: 1753 Fragments arranged by size: 3114 1753 958 542 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7342 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 2861 3119 3343 3944 6002 1239 Size: 819 803 258 224 601 2058 2579 Fragments arranged by size: 2579 2058 819 803 601 258 224 Bpu10I CC'TnA_GC Cuts at: 3859 4245 3859 Size: 386 6956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2727 5467 6332 6630 2727 Size: 2740 865 298 3439 Fragments arranged by size: 3439 2740 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6005 882 Size: 617 4506 2219 Fragments arranged by size: 4506 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5133 493 Size: 711 3929 2702 Fragments arranged by size: 3929 2702 711 BsaBI GATnn'nnATC Cuts at: 2573 7336 2573 Size: 4763 2579 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 5149 5531 6166 Size: 53 83 186 936 603 3013 382 635 Cuts at: 6166 275 Size: 1451 Fragments arranged by size: 3013 1451 936 635 603 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2479 5775 6173 6447 6594 7005 2479 Size: 3296 398 274 147 411 2816 Fragments arranged by size: 3296 2816 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7117 Fragments arranged by size: 7117 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2055 2072 3909 1186 Size: 869 17 1837 4619 Fragments arranged by size: 4619 1837 869 17 BseYI C'CCAG_C Cuts at: 4560 6545 7041 4560 Size: 1985 496 4861 Fragments arranged by size: 4861 1985 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3488 4455 1163 Size: 573 1752 967 4050 Fragments arranged by size: 4050 1752 967 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4792 5553 6581 1540 Size: 275 186 2791 761 1028 2301 Fragments arranged by size: 2791 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3315 3492 5403 5488 6559 3315 Size: 177 1911 85 1071 4098 Fragments arranged by size: 4098 1911 1071 177 85 BsmI GAATG_Cn' Cuts at: 2850 2957 3034 4853 4946 2850 Size: 107 77 1819 93 5246 Fragments arranged by size: 5246 1819 107 93 77 BsmBI CGTCTCn'nnnn_ Cuts at: 3065 3065 Size: 7342 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3011 3136 3315 3492 4267 4294 4661 Size: 399 1221 125 179 177 775 27 367 Cuts at: 4661 5403 5488 6559 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1221 1071 775 742 399 367 179 177 125 85 27 BspEI T'CCGG_A Cuts at: 7005 7005 Size: 7342 BspHI T'CATG_A Cuts at: 5231 6961 5231 Size: 1730 5612 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4697 844 Size: 3853 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5229 1394 Size: 429 3406 3507 Fragments arranged by size: 3507 3406 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4050 5837 6011 65 Size: 1183 2802 1787 174 1396 Fragments arranged by size: 2802 1787 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5986 6173 1700 Size: 110 4176 187 2869 Fragments arranged by size: 4176 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3886 96 Size: 3790 3552 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7342 BssSI C'ACGA_G Cuts at: 5402 6414 5402 Size: 1012 6330 BstAPI GCAn_nnn'nTGC Cuts at: 794 3056 794 Size: 2262 5080 BstBI TT'CG_AA Cuts at: 3970 6146 3970 Size: 2176 5166 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7342 BstYI r'GATC_y Cuts at: 1972 2008 3094 3820 4109 4672 5039 5424 5441 Size: 36 1086 726 289 563 367 385 17 Cuts at: 5441 6893 6979 6991 7337 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1086 726 563 385 367 346 289 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 4080 4267 513 Size: 552 939 12 2064 187 3588 Fragments arranged by size: 3588 2064 939 552 187 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6603 Fragments arranged by size: 6603 683 56 BtsI GCAGTG_nn' Cuts at: 2085 2300 3136 4858 5652 5672 2085 Size: 215 836 1722 794 20 3755 Fragments arranged by size: 3755 1722 836 794 215 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7342 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7307 DraI TTT'AAA Cuts at: 3333 4646 3333 Size: 1313 6029 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6349 809 Size: 507 5033 1802 Fragments arranged by size: 5033 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2895 4394 4789 Size: 54 1425 50 275 18 1065 1499 395 Cuts at: 4789 5678 7307 8 Size: 889 1629 43 Fragments arranged by size: 1629 1499 1425 1065 889 395 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4789 1537 Size: 275 2977 4090 Fragments arranged by size: 4090 2977 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2388 3303 4169 4180 4511 5272 1353 Size: 1035 915 866 11 331 761 3423 Fragments arranged by size: 3423 1035 915 866 761 331 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5929 6303 6449 387 Size: 1064 138 4340 374 146 1280 Fragments arranged by size: 4340 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2439 2861 3119 3327 3343 3944 Size: 393 426 381 422 258 208 16 601 Cuts at: 3944 4492 5405 6002 6789 7057 1239 Size: 548 913 597 787 268 1524 Fragments arranged by size: 1524 913 787 601 597 548 426 422 393 381 268 258 208 16 EcoNI CCTnn'n_nnAGG Cuts at: 2040 2040 Size: 7342 EcoO109I rG'GnC_Cy Cuts at: 3472 3914 4263 3472 Size: 442 349 6551 Fragments arranged by size: 6551 442 349 EcoRI G'AATT_C Cuts at: 2105 4215 4326 4651 5029 2105 Size: 2110 111 325 378 4418 Fragments arranged by size: 4418 2110 378 325 111 EcoRV GAT'ATC Cuts at: 2575 3303 4387 2575 Size: 728 1084 5530 Fragments arranged by size: 5530 1084 728 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3332 3842 3332 Size: 510 6832 FspI TGC'GCA Cuts at: 3049 5848 3049 Size: 2799 4543 HaeII r_GCGC'y Cuts at: 1678 2139 6489 1678 Size: 461 4350 2531 Fragments arranged by size: 4350 2531 461 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2442 2474 4123 Size: 32 135 32 773 32 65 32 1649 Cuts at: 4123 4155 5983 6015 6057 6089 1373 Size: 32 1828 32 42 32 2626 Fragments arranged by size: 2626 1828 1649 773 135 65 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3355 3500 4686 4932 669 Size: 988 281 1417 145 1186 246 3079 Fragments arranged by size: 3079 1417 1186 988 281 246 145 HindIII A'AGCT_T Cuts at: 748 748 Size: 7342 HpaI GTT'AAC Cuts at: 4932 4932 Size: 7342 KasI G'GCGC_C Cuts at: 2135 2135 Size: 7342 KpnI G_GTAC'C Cuts at: 4667 4667 Size: 7342 MfeI C'AATT_G Cuts at: 2673 4941 2673 Size: 2268 5074 MluI A'CGCG_T Cuts at: 7218 7218 Size: 7342 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2859 3005 6456 6640 1305 Size: 1554 146 3451 184 2007 Fragments arranged by size: 3451 2007 1554 184 146 MscI TGG'CCA Cuts at: 10 64 1489 1832 2897 4396 10 Size: 54 1425 343 1065 1499 2956 Fragments arranged by size: 2956 1499 1425 1065 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2376 2451 3990 5300 5659 5818 518 Size: 648 1210 75 1539 1310 359 159 2042 Fragments arranged by size: 2042 1539 1310 1210 648 359 159 75 MspA1I CmG'CkG Cuts at: 1606 2018 4758 5435 6583 6828 1606 Size: 412 2740 677 1148 245 2120 Fragments arranged by size: 2740 2120 1148 677 412 245 NarI GG'CG_CC Cuts at: 2136 2136 Size: 7342 NcoI C'CATG_G Cuts at: 513 1065 2004 4080 4267 513 Size: 552 939 2076 187 3588 Fragments arranged by size: 3588 2076 939 552 187 NdeI CA'TA_TG Cuts at: 387 387 Size: 7342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7342 NotI GC'GGCC_GC Cuts at: 4789 4789 Size: 7342 NsiI A_TGCA'T Cuts at: 3997 3997 Size: 7342 NspI r_CATG'y Cuts at: 1132 1229 4700 6245 1132 Size: 97 3471 1545 2229 Fragments arranged by size: 3471 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7342 PciI A'CATG_T Cuts at: 6241 6241 Size: 7342 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7342 PfoI T'CCnGG_A Cuts at: 3724 3724 Size: 7342 PmeI GTTT'AAAC Cuts at: 4646 4646 Size: 7342 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5411 5443 6924 6956 5411 Size: 32 1481 32 5797 Fragments arranged by size: 5797 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3472 3914 3472 Size: 442 6900 PshAI GACnn'nnGTC Cuts at: 1613 3249 3360 1613 Size: 1636 111 5595 Fragments arranged by size: 5595 1636 111 PsiI TTA'TAA Cuts at: 4912 4912 Size: 7342 PspOMI G'GGCC_C Cuts at: 3007 4263 3007 Size: 1256 6086 PstI C_TGCA'G Cuts at: 830 2307 4536 4694 830 Size: 1477 2229 158 3478 Fragments arranged by size: 3478 2229 1477 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7342 SacII CC_GC'GG Cuts at: 2019 2019 Size: 7342 SalI G'TCGA_C Cuts at: 1936 4684 1936 Size: 2748 4594 SapI GCTCTTCn'nnn_ Cuts at: 3303 3303 Size: 7342 SbfI CC_TGCA'GG Cuts at: 4694 4694 Size: 7342 ScaI AGT'ACT Cuts at: 5590 7302 5590 Size: 1712 5630 SfcI C'TryA_G Cuts at: 826 1045 2303 2766 4063 4532 4690 5825 6506 Size: 219 1258 463 1297 469 158 1135 681 Cuts at: 6506 6697 826 Size: 191 1471 Fragments arranged by size: 1471 1297 1258 1135 681 469 463 219 191 158 SfoI GGC'GCC Cuts at: 2137 2137 Size: 7342 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7342 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7342 SmaI CCC'GGG Cuts at: 1872 4669 1872 Size: 2797 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2742 3476 3649 5446 6347 6609 Size: 197 926 799 734 173 1797 901 262 Cuts at: 6609 820 Size: 1553 Fragments arranged by size: 1797 1553 926 901 799 734 262 197 173 SnaBI TAC'GTA Cuts at: 493 5133 493 Size: 4640 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7342 SphI G_CATG'C Cuts at: 1132 4700 1132 Size: 3568 3774 SspI AAT'ATT Cuts at: 5 52 3697 3814 4372 5266 5 Size: 47 3645 117 558 894 2081 Fragments arranged by size: 3645 2081 894 558 117 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7342 StyI C'CwwG_G Cuts at: 513 1065 2004 3316 4080 4267 4391 4781 513 Size: 552 939 1312 764 187 124 390 3074 Fragments arranged by size: 3074 1312 939 764 552 390 187 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5369 5386 5539 5724 6169 325 Size: 1255 3789 17 153 185 445 1498 Fragments arranged by size: 3789 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3886 5588 7300 Size: 274 80 33 51 1443 1909 1702 1712 Cuts at: 7300 96 Size: 138 Fragments arranged by size: 1909 1712 1702 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2835 2867 4040 4072 7302 7334 2835 Size: 32 1173 32 3230 32 2843 Fragments arranged by size: 3230 2843 1173 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7342 XbaI T'CTAG_A Cuts at: 2434 4678 2434 Size: 2244 5098 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7342 XmaI C'CCGG_G Cuts at: 1870 4667 1870 Size: 2797 4545 XmnI GAAnn'nnTTC Cuts at: 3080 5471 3080 Size: 2391 4951 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5150 276 Size: 53 83 186 936 3616 2468 Fragments arranged by size: 3616 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI