(Linear) (Six-base) MAPSORT of: KIEE3317.seq Check: 2203 from: 1 to: 2637 KIEE3317.orf With 182 enzymes: SgfI * January 29, 2014 11:45 .. AccI GT'mk_AC Cuts at: 0 317 2276 2637 Size: 317 1959 361 Fragments arranged by size: 1959 361 317 AclI AA'CG_TT Cuts at: 0 1933 2637 Size: 1933 704 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 434 1322 2487 2637 Size: 434 888 1165 150 Fragments arranged by size: 1165 888 434 150 AflII C'TTAA_G Cuts at: 0 1471 1644 2637 Size: 1471 173 993 Fragments arranged by size: 1471 993 173 AlwNI CAG_nnn'CTG Cuts at: 0 2613 2637 Size: 2613 24 ApaI G_GGCC'C Cuts at: 0 1006 2262 2637 Size: 1006 1256 375 Fragments arranged by size: 1256 1006 375 ApaLI G'TGCA_C Cuts at: 0 1483 2637 Size: 1483 1154 ApoI r'AATT_y Cuts at: 0 100 1335 1724 2210 2308 2321 2637 Size: 100 1235 389 486 98 13 316 Fragments arranged by size: 1235 486 389 316 100 98 13 AseI AT'TA_AT Cuts at: 0 557 1985 2637 Size: 557 1428 652 Fragments arranged by size: 1428 652 557 BamHI G'GATC_C Cuts at: 0 3 2637 Size: 3 2634 BanI G'GyrC_C Cuts at: 0 130 2637 Size: 130 2507 BanII G_rGCy'C Cuts at: 0 1006 2262 2637 Size: 1006 1256 375 Fragments arranged by size: 1256 1006 375 BbeI G_GCGC'C Cuts at: 0 134 2637 Size: 134 2503 BbsI GAAGACnn'nnnn_ Cuts at: 0 1619 2637 Size: 1619 1018 BbvCI CC'TCA_GC Cuts at: 0 1854 2637 Size: 1854 783 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1858 1892 2637 Size: 1858 34 745 Fragments arranged by size: 1858 745 34 BclI T'GATC_A Cuts at: 0 206 2637 Size: 206 2431 BfrBI ATG'CAT Cuts at: 0 1990 2637 Size: 1990 647 BlpI GC'TnA_GC Cuts at: 0 2606 2637 Size: 2606 31 Bme1580I G_kGCm'C Cuts at: 0 1006 1131 1487 2262 2289 2637 Size: 1006 125 356 775 27 348 Fragments arranged by size: 1006 775 356 348 125 27 BmrI ACTGGGnnnn_n' Cuts at: 0 2526 2637 Size: 2526 111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 53 856 1114 1338 1939 2637 Size: 53 803 258 224 601 698 Fragments arranged by size: 803 698 601 258 224 53 Bpu10I CC'TnA_GC Cuts at: 0 1854 2240 2637 Size: 1854 386 397 Fragments arranged by size: 1854 397 386 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 722 2637 Size: 722 1915 BsaBI GATnn'nnATC Cuts at: 0 568 2637 Size: 568 2069 BsaHI Gr'CG_yC Cuts at: 0 131 2637 Size: 131 2506 BsaWI w'CCGG_w Cuts at: 0 474 2637 Size: 474 2163 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 50 67 1904 2637 Size: 50 17 1837 733 Fragments arranged by size: 1837 733 50 17 BseYI C'CCAG_C Cuts at: 0 2555 2637 Size: 2555 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1483 2450 2637 Size: 1483 967 187 Fragments arranged by size: 1483 967 187 BsiHKAI G_wGCw'C Cuts at: 0 1310 1487 2637 Size: 1310 177 1150 Fragments arranged by size: 1310 1150 177 BsmI GAATG_Cn' Cuts at: 0 845 952 1029 2637 Size: 845 107 77 1608 Fragments arranged by size: 1608 845 107 77 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1060 2637 Size: 1060 1577 Bsp1286I G_dGCh'C Cuts at: 0 1006 1131 1310 1487 2262 2289 2637 Size: 1006 125 179 177 775 27 348 Fragments arranged by size: 1006 775 348 179 177 125 27 BsrDI GCAATG_nn' Cuts at: 0 2045 2637 Size: 2045 592 BsrGI T'GTAC_A Cuts at: 0 1881 2637 Size: 1881 756 BstAPI GCAn_nnn'nTGC Cuts at: 0 1051 2637 Size: 1051 1586 BstBI TT'CG_AA Cuts at: 0 1965 2637 Size: 1965 672 BstYI r'GATC_y Cuts at: 0 3 1089 1815 2104 2637 Size: 3 1086 726 289 533 Fragments arranged by size: 1086 726 533 289 3 BtgI C'CryG_G Cuts at: 0 11 2075 2262 2637 Size: 11 2064 187 375 Fragments arranged by size: 2064 375 187 11 BtsI GCAGTG_nn' Cuts at: 0 80 295 1131 2637 Size: 80 215 836 1506 Fragments arranged by size: 1506 836 215 80 DraI TTT'AAA Cuts at: 0 1328 2637 Size: 1328 1309 EaeI y'GGCC_r Cuts at: 0 890 2389 2637 Size: 890 1499 248 Fragments arranged by size: 1499 890 248 EarI CTCTTCn'nnn_ Cuts at: 0 383 1298 2164 2175 2506 2637 Size: 383 915 866 11 331 131 Fragments arranged by size: 915 866 383 331 131 11 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 53 434 856 1114 1322 1338 1939 2487 Size: 53 381 422 258 208 16 601 548 Cuts at: 2487 2637 Size: 150 Fragments arranged by size: 601 548 422 381 258 208 150 53 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 35 2637 Size: 35 2602 EcoO109I rG'GnC_Cy Cuts at: 0 1467 1909 2258 2637 Size: 1467 442 349 379 Fragments arranged by size: 1467 442 379 349 EcoRI G'AATT_C Cuts at: 0 100 2210 2321 2637 Size: 100 2110 111 316 Fragments arranged by size: 2110 316 111 100 EcoRV GAT'ATC Cuts at: 0 570 1298 2382 2637 Size: 570 728 1084 255 Fragments arranged by size: 1084 728 570 255 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1327 1837 2637 Size: 1327 510 800 Fragments arranged by size: 1327 800 510 FspI TGC'GCA Cuts at: 0 1044 2637 Size: 1044 1593 HaeII r_GCGC'y Cuts at: 0 134 2637 Size: 134 2503 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 340 372 437 469 2118 2150 2637 Size: 340 32 65 32 1649 32 487 Fragments arranged by size: 1649 487 340 65 32 32 32 HincII GTy'rAC Cuts at: 0 1350 1495 2637 Size: 1350 145 1142 Fragments arranged by size: 1350 1142 145 KasI G'GCGC_C Cuts at: 0 130 2637 Size: 130 2507 MfeI C'AATT_G Cuts at: 0 668 2637 Size: 668 1969 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 854 1000 2637 Size: 854 146 1637 Fragments arranged by size: 1637 854 146 MscI TGG'CCA Cuts at: 0 892 2391 2637 Size: 892 1499 246 Fragments arranged by size: 1499 892 246 MslI CAynn'nnrTG Cuts at: 0 371 446 1985 2637 Size: 371 75 1539 652 Fragments arranged by size: 1539 652 371 75 MspA1I CmG'CkG Cuts at: 0 13 2637 Size: 13 2624 NarI GG'CG_CC Cuts at: 0 131 2637 Size: 131 2506 NcoI C'CATG_G Cuts at: 0 2075 2262 2637 Size: 2075 187 375 Fragments arranged by size: 2075 375 187 NsiI A_TGCA'T Cuts at: 0 1992 2637 Size: 1992 645 PfoI T'CCnGG_A Cuts at: 0 1719 2637 Size: 1719 918 PpuMI rG'GwC_Cy Cuts at: 0 1467 1909 2637 Size: 1467 442 728 Fragments arranged by size: 1467 728 442 PshAI GACnn'nnGTC Cuts at: 0 1244 1355 2637 Size: 1244 111 1282 Fragments arranged by size: 1282 1244 111 PspOMI G'GGCC_C Cuts at: 0 1002 2258 2637 Size: 1002 1256 379 Fragments arranged by size: 1256 1002 379 PstI C_TGCA'G Cuts at: 0 302 2531 2637 Size: 302 2229 106 Fragments arranged by size: 2229 302 106 SacII CC_GC'GG Cuts at: 0 14 2637 Size: 14 2623 SapI GCTCTTCn'nnn_ Cuts at: 0 1298 2637 Size: 1298 1339 SfcI C'TryA_G Cuts at: 0 298 761 2058 2527 2637 Size: 298 463 1297 469 110 Fragments arranged by size: 1297 469 463 298 110 SfoI GGC'GCC Cuts at: 0 132 2637 Size: 132 2505 SmlI C'TyrA_G Cuts at: 0 737 1471 1644 2637 Size: 737 734 173 993 Fragments arranged by size: 993 737 734 173 SspI AAT'ATT Cuts at: 0 1692 1809 2367 2637 Size: 1692 117 558 270 Fragments arranged by size: 1692 558 270 117 StyI C'CwwG_G Cuts at: 0 1311 2075 2262 2386 2637 Size: 1311 764 187 124 251 Fragments arranged by size: 1311 764 251 187 124 TatI w'GTAC_w Cuts at: 0 1881 2637 Size: 1881 756 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 830 862 2035 2067 2637 Size: 830 32 1173 32 570 Fragments arranged by size: 1173 830 570 32 32 XbaI T'CTAG_A Cuts at: 0 429 2637 Size: 429 2208 XmnI GAAnn'nnTTC Cuts at: 0 1075 2637 Size: 1075 1562 Enzymes that do cut: AccI AclI AcuI AflII AlwNI ApaI ApaLI ApoI AseI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrGI BstAPI BstBI BstYI BtgI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NsiI PfoI PpuMI PshAI PspOMI PstI SacII SapI SfcI SfoI SmlI SspI StyI TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflIII AgeI AhdI AleI AlfI AloI AscI AsiSI AvaI AvrII BaeI BciVI BglI BglII BmgBI BmtI BplI BsaI BsaAI BsaXI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI FseI FspAI HindIII HpaI KpnI MluI NaeI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I XcmI XhoI XmaI ZraI