################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbsI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MroI MslI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcsI 127 AAATTC [GA]^AATT[CT] AcyI 774 GGCGCC G[GA]^CG[CT]C AgeI 1007 ACCGGT A^CCGGT AlwI 879 GATCC GGATC(4/5) AocI 714 CCTGAGG CC^T[ACGT]AGG ApoI 127 AAATTC [GA]^AATT[CT] AspHI 571 GAGCTC G[AT]GC[AT]^C BalI 593 TGGCCA TGG^CCA BbeI 774 GGCGCC GGCGC^C BbrPI 547 CACGTG CAC^GTG BbvCI 140 CCTCAGC CCTCAGC(-5/-2) BclI 236 TGATCA T^GATCA BfaI 292 CTAG C^TAG BsaHI 774 GGCGCC G[GA]^CG[CT]C BsaWI 1007 ACCGGT [AT]^CCGG[AT] BsiEI 1005 CGACCG CG[GA][CT]^CG BsiHKAI 571 GAGCTC G[AT]GC[AT]^C Bsp1407I 188 TGTACA T^GTACA BspMI 490 ACCTGC ACCTGC(4/8) BsrGI 188 TGTACA T^GTACA BssSI 634 CTCGTG CACGAG(-5/-1) BstYI 878 AGATCC [GA]^GATC[CT] Bsu36I 714 CCTGAGG CC^T[ACGT]AGG CfoI 775 GCGC GCG^C Eco81I 714 CCTGAGG CC^T[ACGT]AGG FbaI 236 TGATCA T^GATCA HaeII 774 GGCGCC [GA]GCGC^[CT] HhaI 775 GCGC GCG^C Hin1I 774 GGCGCC G[GA]^CG[CT]C HinP1I 775 GCGC G^CGC KasI 774 GGCGCC G^GCGCC MaeI 292 CTAG C^TAG MflI 878 AGATCC [GA]^GATC[CT] MluNI 593 TGGCCA TGG^CCA MscI 593 TGGCCA TGG^CCA MseI 5 TTAA T^TAA NarI 774 GGCGCC GG^CGCC PinAI 1007 ACCGGT A^CCGGT PmaCI 547 CACGTG CAC^GTG PmlI 547 CACGTG CAC^GTG PshAI 625 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 205 CTGCAG CTGCA^G SacI 571 GAGCTC GAGCT^C ScaI 164 AGTACT AGT^ACT SfcI 205 CTGCAG C^T[GA][CT]AG SfoI 774 GGCGCC GGC^GCC SmaI 820 CCCGGG CCC^GGG SmlI 946 CTCAAG C^T[CT][GA]AG SspBI 188 TGTACA T^GTACA Tru9I 5 TTAA T^TAA Tsp509I 128 AATT ^AATT TspRI 495 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 539 CCAAGCTCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 878 AGATCC [GA]^GATC[CT] XmaCI 820 CCCGGG C^CCGGG XmaI 820 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 313 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 799 CAGCAGCTG BanI 119 GGCACC G^G[CT][GA]CC 774 GGCGCC BpmI 840 CTCCAG CTGGAG(16/14) 955 CTGGAG BsaAI 547 CACGTG [CT]AC^GT[GA] 664 TACGTG BsaI 631 GGTCTC GGTCTC(1/5) 837 GGTCTC BsrFI 938 ACCGGC [GA]^CCGG[CT] 1007 ACCGGT BstEII 486 GGTCACC G^GT[ACGT]ACC 1074 GGTCACC BstPI 486 GGTCACC G^GT[ACGT]ACC 1074 GGTCACC Cfr10I 938 ACCGGC [GA]^CCGG[CT] 1007 ACCGGT DraIII 240 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 852 CACCAAGTG DsaI 924 CCACGG C^C[GA][CT]GG 1109 CCGTGG EaeI 593 TGGCCA [CT]^GGCC[GA] 600 CGGCCA EcoO65I 486 GGTCACC G^GT[ACGT]ACC 1074 GGTCACC EcoT14I 671 CCTTGG C^C[AT][AT]GG 971 CCAAGG HinfI 22 GACTC G^A[ACGT]TC 263 GACTC PflMI 798 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 924 CCACGGGCTGG PleI 22 GACTC GAGTC(4/5) 263 GACTC StyI 671 CCTTGG C^C[AT][AT]GG 971 CCAAGG Van91I 798 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 924 CCACGGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AfaI AluI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I TthHB8I