(Circular) (Six-base) MAPSORT of: pFN21AE5588.seq Check: 7632 from: 1 to: 5836 pFN21AE5588 5836 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2504 2504 Size: 5836 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3899 5283 5551 1632 Size: 2267 1384 268 1917 Fragments arranged by size: 2267 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 3012 4667 3012 Size: 1655 4181 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 2323 2809 3579 5151 2323 Size: 486 770 1572 3008 Fragments arranged by size: 3008 1572 770 486 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 2132 3145 3371 3523 5632 1493 Size: 639 1013 226 152 2109 1697 Fragments arranged by size: 2109 1697 1013 639 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2056 2684 2759 2783 2825 3161 1870 Size: 73 113 628 75 24 42 336 4545 Fragments arranged by size: 4545 628 336 113 75 73 42 24 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2124 2779 3157 4612 618 Size: 325 217 627 337 655 378 1455 1842 Fragments arranged by size: 1842 1455 655 627 378 337 325 217 BanII G_rGCy'C Cuts at: 1391 2340 2580 2685 2825 2832 3155 1391 Size: 949 240 105 140 7 323 4072 Fragments arranged by size: 4072 949 323 240 140 105 7 BbeI G_GCGC'C Cuts at: 2783 2783 Size: 5836 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4865 BbvCI CC'TCA_GC Cuts at: 2146 2146 Size: 5836 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4027 4061 1903 Size: 34 2090 34 3678 Fragments arranged by size: 3678 2090 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 2241 1548 Size: 693 5143 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4447 136 Size: 107 122 71 631 605 2775 1525 Fragments arranged by size: 2775 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 3253 3253 Size: 5836 Bme1580I G_kGCm'C Cuts at: 1790 2127 2406 2802 2918 3897 5053 1790 Size: 337 279 396 116 979 1156 2573 Fragments arranged by size: 2573 1156 979 396 337 279 116 BmgBI CAC'GTC Cuts at: 1318 2260 1318 Size: 942 4894 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2539 2539 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2830 2981 4496 1239 Size: 1591 151 1515 2579 Fragments arranged by size: 2579 1591 1515 151 Bpu10I CC'TnA_GC Cuts at: 2146 2146 Size: 5836 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2936 3961 4826 5124 2936 Size: 1025 865 298 3648 Fragments arranged by size: 3648 1025 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2642 2848 4499 882 Size: 617 1143 206 1651 2219 Fragments arranged by size: 2219 1651 1143 617 206 BsaAI yAC'GTr Cuts at: 493 1204 2554 2671 3627 493 Size: 711 1350 117 956 2702 Fragments arranged by size: 2702 1350 956 711 117 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2780 3643 4025 4660 Size: 53 83 186 936 1247 863 382 635 Cuts at: 4660 275 Size: 1451 Fragments arranged by size: 1451 1247 936 863 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3012 4269 4667 4941 5088 5499 3012 Size: 1257 398 274 147 411 3349 Fragments arranged by size: 3349 1257 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2177 2207 1178 Size: 30 165 30 774 30 4807 Fragments arranged by size: 4807 774 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 2570 2726 1186 Size: 1012 372 156 4296 Fragments arranged by size: 4296 1012 372 156 BseYI C'CCAG_C Cuts at: 2309 2802 2809 5039 5535 2309 Size: 493 7 2230 496 2610 Fragments arranged by size: 2610 2230 496 493 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2201 2482 2756 3140 1163 Size: 573 465 281 274 384 3859 Fragments arranged by size: 3859 573 465 384 281 274 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3013 3286 4047 5075 1540 Size: 275 186 1012 273 761 1028 2301 Fragments arranged by size: 2301 1028 1012 761 275 273 186 BsiHKAI G_wGCw'C Cuts at: 2580 3897 3982 5053 2580 Size: 1317 85 1071 3363 Fragments arranged by size: 3363 1317 1071 85 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2340 2406 2580 2685 2802 2825 Size: 399 337 213 66 174 105 117 23 Cuts at: 2825 2832 2918 3155 3897 3982 5053 1391 Size: 7 86 237 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 337 237 213 174 117 105 86 85 66 23 7 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2504 3191 844 Size: 1660 687 3489 Fragments arranged by size: 3489 1660 687 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4331 4505 65 Size: 1183 3083 174 1396 Fragments arranged by size: 3083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2943 3012 4480 4667 1700 Size: 110 1133 69 1468 187 2869 Fragments arranged by size: 2869 1468 1133 187 110 69 BsrGI T'GTAC_A Cuts at: 96 2193 96 Size: 2097 3739 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 2639 3896 4908 2639 Size: 1257 1012 3567 Fragments arranged by size: 3567 1257 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5836 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 2491 3079 1184 Size: 1307 588 3941 Fragments arranged by size: 3941 1307 588 BstYI r'GATC_y Cuts at: 1972 2883 3166 3533 3918 3935 5387 5473 5485 Size: 911 283 367 385 17 1452 86 12 Cuts at: 5485 5831 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 911 385 367 346 283 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2720 2720 Size: 5836 BtgI C'CryG_G Cuts at: 513 1065 2929 3114 513 Size: 552 1864 185 3235 Fragments arranged by size: 3235 1864 552 185 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 3352 4146 4166 3352 Size: 794 20 5022 Fragments arranged by size: 5022 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3013 3048 529 Size: 35 2449 35 3317 Fragments arranged by size: 3317 2449 35 35 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DraIII CAC_nnn'GTG Cuts at: 2250 2862 2250 Size: 612 5224 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2598 2605 3283 Size: 54 1425 50 275 18 768 7 678 Cuts at: 3283 4172 5801 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 768 678 275 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3766 1353 Size: 2413 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4423 4797 4943 387 Size: 1064 138 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2578 2578 Size: 5836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2830 2981 3899 4496 5283 5551 1239 Size: 393 1198 151 918 597 787 268 1524 Fragments arranged by size: 1524 1198 918 787 597 393 268 151 EcoO109I rG'GnC_Cy Cuts at: 2188 2206 2716 2764 2788 3108 2188 Size: 18 510 48 24 320 4916 Fragments arranged by size: 4916 510 320 48 24 18 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2299 2905 2188 Size: 111 606 5119 Fragments arranged by size: 5119 606 111 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 2783 4983 1678 Size: 1105 2200 2531 Fragments arranged by size: 2531 2200 1105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2986 3018 3027 Size: 32 135 32 467 32 915 32 9 Cuts at: 3027 3059 4477 4509 4551 4583 1373 Size: 32 1418 32 42 32 2626 Fragments arranged by size: 2626 1418 915 467 135 42 32 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KasI G'GCGC_C Cuts at: 2779 2779 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2400 4950 5134 1305 Size: 1095 2550 184 2007 Fragments arranged by size: 2550 2007 1095 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2600 10 Size: 54 1425 343 768 3246 Fragments arranged by size: 3246 1425 768 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3794 4153 4312 518 Size: 648 2628 359 159 2042 Fragments arranged by size: 2628 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2215 2481 2590 2604 2626 2770 2809 2968 Size: 609 266 109 14 22 144 39 159 Cuts at: 2968 3252 3929 5077 5322 1606 Size: 284 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 609 284 266 245 159 144 109 39 22 14 NarI GG'CG_CC Cuts at: 2780 2780 Size: 5836 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5284 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 2352 1348 Size: 1004 4832 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2809 2935 1108 Size: 1701 126 4009 Fragments arranged by size: 4009 1701 126 PfoI T'CCnGG_A Cuts at: 2699 2699 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PmlI CAC'GTG Cuts at: 2554 2554 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2777 2809 3905 3937 5418 5450 2777 Size: 32 1096 32 1481 32 3163 Fragments arranged by size: 3163 1481 1096 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2716 2764 3108 2716 Size: 48 344 5444 Fragments arranged by size: 5444 344 48 PshAI GACnn'nnGTC Cuts at: 1613 2634 1613 Size: 1021 4815 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 2214 3188 830 Size: 1384 974 3478 Fragments arranged by size: 3478 1384 974 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2215 2590 2626 2809 2968 2215 Size: 375 36 183 159 5083 Fragments arranged by size: 5083 375 183 159 36 SacI G_AGCT'C Cuts at: 2580 2580 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SanDI GG'GwC_CC Cuts at: 2764 2764 Size: 5836 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 2171 4084 5796 2171 Size: 1913 1712 2211 Fragments arranged by size: 2211 1913 1712 SfcI C'TryA_G Cuts at: 826 1045 2210 3184 4319 5000 5191 826 Size: 219 1165 974 1135 681 191 1471 Fragments arranged by size: 1471 1165 1135 974 681 219 191 SfoI GGC'GCC Cuts at: 2781 2781 Size: 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 2827 3163 1872 Size: 955 336 4545 Fragments arranged by size: 4545 955 336 SmlI C'TyrA_G Cuts at: 820 1017 1943 2951 3940 4841 5103 820 Size: 197 926 1008 989 901 262 1553 Fragments arranged by size: 1553 1008 989 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 2676 2976 3275 513 Size: 552 1611 300 299 3074 Fragments arranged by size: 3074 1611 552 300 299 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3863 3880 4033 4218 4663 325 Size: 1255 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2169 2193 2727 Size: 274 80 33 51 1443 192 24 534 Cuts at: 2727 4082 5794 96 Size: 1355 1712 138 Fragments arranged by size: 1712 1443 1355 534 274 192 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5796 5828 5796 Size: 32 5804 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2551 2551 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 2825 3161 1870 Size: 955 336 4545 Fragments arranged by size: 4545 955 336 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI