(Linear) (Six-base) MAPSORT of: KIEE5588.seq Check: 472 from: 1 to: 1131 KIEE5588.orf With 182 enzymes: SgfI * January 29, 2014 12:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 499 1131 Size: 499 632 AgeI A'CCGG_T Cuts at: 0 1007 1131 Size: 1007 124 AlwNI CAG_nnn'CTG Cuts at: 0 318 804 1131 Size: 318 486 327 Fragments arranged by size: 486 327 318 ApoI r'AATT_y Cuts at: 0 127 1131 Size: 127 1004 AvaI C'yCGr_G Cuts at: 0 51 679 754 778 820 1131 Size: 51 628 75 24 42 311 Fragments arranged by size: 628 311 75 51 42 24 BanI G'GyrC_C Cuts at: 0 119 774 1131 Size: 119 655 357 Fragments arranged by size: 655 357 119 BanII G_rGCy'C Cuts at: 0 335 575 680 820 827 1131 Size: 335 240 105 140 7 304 Fragments arranged by size: 335 304 240 140 105 7 BbeI G_GCGC'C Cuts at: 0 778 1131 Size: 778 353 BbvCI CC'TCA_GC Cuts at: 0 141 1131 Size: 141 990 BclI T'GATC_A Cuts at: 0 236 1131 Size: 236 895 Bme1580I G_kGCm'C Cuts at: 0 122 401 797 913 1131 Size: 122 279 396 116 218 Fragments arranged by size: 396 279 218 122 116 BmgBI CAC'GTC Cuts at: 0 255 1131 Size: 255 876 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 534 1131 Size: 534 597 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 825 976 1131 Size: 825 151 155 Fragments arranged by size: 825 155 151 Bpu10I CC'TnA_GC Cuts at: 0 141 1131 Size: 141 990 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 931 1131 Size: 931 200 BsaI GGTCTCn'nnnn_ Cuts at: 0 637 843 1131 Size: 637 206 288 Fragments arranged by size: 637 288 206 BsaAI yAC'GTr Cuts at: 0 549 666 1131 Size: 549 117 465 Fragments arranged by size: 549 465 117 BsaHI Gr'CG_yC Cuts at: 0 775 1131 Size: 775 356 BsaWI w'CCGG_w Cuts at: 0 1007 1131 Size: 1007 124 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 172 202 1131 Size: 172 30 929 Fragments arranged by size: 929 172 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 193 565 721 1131 Size: 193 372 156 410 Fragments arranged by size: 410 372 193 156 BseYI C'CCAG_C Cuts at: 0 304 797 804 1131 Size: 304 493 7 327 Fragments arranged by size: 493 327 304 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 477 751 1131 Size: 196 281 274 380 Fragments arranged by size: 380 281 274 196 BsiEI CG_ry'CG Cuts at: 0 1008 1131 Size: 1008 123 BsiHKAI G_wGCw'C Cuts at: 0 575 1131 Size: 575 556 Bsp1286I G_dGCh'C Cuts at: 0 122 335 401 575 680 797 820 827 Size: 122 213 66 174 105 117 23 7 Cuts at: 827 913 1131 Size: 86 218 Fragments arranged by size: 218 213 174 122 117 105 86 66 23 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 499 1131 Size: 499 632 BsrFI r'CCGG_y Cuts at: 0 938 1007 1131 Size: 938 69 124 Fragments arranged by size: 938 124 69 BsrGI T'GTAC_A Cuts at: 0 188 1131 Size: 188 943 BssSI C'ACGA_G Cuts at: 0 634 1131 Size: 634 497 BstEII G'GTnAC_C Cuts at: 0 486 1074 1131 Size: 486 588 57 Fragments arranged by size: 588 486 57 BstYI r'GATC_y Cuts at: 0 878 1131 Size: 878 253 Bsu36I CC'TnA_GG Cuts at: 0 715 1131 Size: 715 416 BtgI C'CryG_G Cuts at: 0 924 1109 1131 Size: 924 185 22 Fragments arranged by size: 924 185 22 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1008 1043 1131 Size: 1008 35 88 Fragments arranged by size: 1008 88 35 DraIII CAC_nnn'GTG Cuts at: 0 245 857 1131 Size: 245 612 274 Fragments arranged by size: 612 274 245 EaeI y'GGCC_r Cuts at: 0 593 600 1131 Size: 593 7 531 Fragments arranged by size: 593 531 7 EcoICRI GAG'CTC Cuts at: 0 573 1131 Size: 573 558 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 825 976 1131 Size: 825 151 155 Fragments arranged by size: 825 155 151 EcoO109I rG'GnC_Cy Cuts at: 0 183 201 711 759 783 1103 1131 Size: 183 18 510 48 24 320 28 Fragments arranged by size: 510 320 183 48 28 24 18 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 183 294 900 1131 Size: 183 111 606 231 Fragments arranged by size: 606 231 183 111 HaeII r_GCGC'y Cuts at: 0 778 1131 Size: 778 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 34 66 981 1013 1022 1054 1131 Size: 34 32 915 32 9 32 77 Fragments arranged by size: 915 77 34 32 32 32 9 KasI G'GCGC_C Cuts at: 0 774 1131 Size: 774 357 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 395 1131 Size: 395 736 MscI TGG'CCA Cuts at: 0 595 1131 Size: 595 536 MspA1I CmG'CkG Cuts at: 0 210 476 585 599 621 765 804 963 Size: 210 266 109 14 22 144 39 159 Cuts at: 963 1131 Size: 168 Fragments arranged by size: 266 210 168 159 144 109 39 22 14 NarI GG'CG_CC Cuts at: 0 775 1131 Size: 775 356 PasI CC'CwG_GG Cuts at: 0 347 1131 Size: 347 784 PflMI CCAn_nnn'nTGG Cuts at: 0 804 930 1131 Size: 804 126 201 Fragments arranged by size: 804 201 126 PfoI T'CCnGG_A Cuts at: 0 694 1131 Size: 694 437 PmlI CAC'GTG Cuts at: 0 549 1131 Size: 549 582 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 772 804 1131 Size: 772 32 327 Fragments arranged by size: 772 327 32 PpuMI rG'GwC_Cy Cuts at: 0 711 759 1103 1131 Size: 711 48 344 28 Fragments arranged by size: 711 344 48 28 PshAI GACnn'nnGTC Cuts at: 0 629 1131 Size: 629 502 PstI C_TGCA'G Cuts at: 0 209 1131 Size: 209 922 PvuII CAG'CTG Cuts at: 0 210 585 621 804 963 1131 Size: 210 375 36 183 159 168 Fragments arranged by size: 375 210 183 168 159 36 SacI G_AGCT'C Cuts at: 0 575 1131 Size: 575 556 SanDI GG'GwC_CC Cuts at: 0 759 1131 Size: 759 372 ScaI AGT'ACT Cuts at: 0 166 1131 Size: 166 965 SfcI C'TryA_G Cuts at: 0 205 1131 Size: 205 926 SfoI GGC'GCC Cuts at: 0 776 1131 Size: 776 355 SmaI CCC'GGG Cuts at: 0 822 1131 Size: 822 309 SmlI C'TyrA_G Cuts at: 0 946 1131 Size: 946 185 StyI C'CwwG_G Cuts at: 0 671 971 1131 Size: 671 300 160 Fragments arranged by size: 671 300 160 TatI w'GTAC_w Cuts at: 0 164 188 722 1131 Size: 164 24 534 409 Fragments arranged by size: 534 409 164 24 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 546 1131 Size: 546 585 XmaI C'CCGG_G Cuts at: 0 820 1131 Size: 820 311 Enzymes that do cut: AarI AgeI AlwNI ApoI AvaI BanI BanII BbeI BbvCI BclI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssSI BstEII BstYI Bsu36I BtgI CspCI DraIII EaeI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI MscI MspA1I NarI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PstI PvuII SacI SanDI ScaI SfcI SfoI SmaI SmlI StyI TatI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BfrBI BglI BglII BlpI BmrI BmtI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI DraI DrdI EagI EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI