################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BseRI BsgI BsiWI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstPI BstUI Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HindIII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 228 GTATAC GT^[AC][GT]AC AcsI 1265 GAATTC [GA]^AATT[CT] AfaI 1305 GTAC GT^AC ApaI 202 GGGCCC GGGCC^C ApoI 1265 GAATTC [GA]^AATT[CT] AseI 790 ATTAAT AT^TAAT AsnI 790 ATTAAT AT^TAAT AspHI 1168 GTGCTC G[AT]GC[AT]^C AvaII 571 GGACC G^G[AT]CC AvrII 51 CCTAGG C^CTAGG BamHI 101 GGATCC G^GATCC BanI 1237 GGCACC G^G[CT][GA]CC BanII 202 GGGCCC G[GA]GC[CT]^C BbsI 940 GTCTTC GAAGAC(2/6) BlnI 51 CCTAGG C^CTAGG BpmI 616 CTCCAG CTGGAG(16/14) BpuAI 940 GTCTTC GAAGAC(2/6) BsaAI 558 TACGTG [CT]AC^GT[GA] BsiEI 142 CGGCCG CG[GA][CT]^CG BsiHKAI 1168 GTGCTC G[AT]GC[AT]^C BsmBI 8 CGTCTC CGTCTC(1/5) BsmI 934 GCATTC GAATGC(1/-1) BspHI 728 TCATGA T^CATGA BsrDI 372 CATTGC GCAATG(2/0) BssSI 1173 CACGAG CACGAG(-5/-1) Bst1107I 228 GTATAC GTA^TAC BstYI 101 GGATCC [GA]^GATC[CT] BstZ17I 228 GTATAC GTA^TAC CfoI 1180 GCGC GCG^C DsaI 905 CCATGG C^C[GA][CT]GG EaeI 142 CGGCCG [CT]^GGCC[GA] EagI 142 CGGCCG C^GGCCG EclXI 142 CGGCCG C^GGCCG Eco52I 142 CGGCCG C^GGCCG EcoRI 1265 GAATTC G^AATTC HaeII 1179 AGCGCC [GA]GCGC^[CT] HhaI 1180 GCGC GCG^C HinP1I 1180 GCGC G^CGC HpaI 481 GTTAAC GTT^AAC MflI 101 GGATCC [GA]^GATC[CT] MspA1I 965 CAGCTG C[AC]G^C[GT]G NcoI 905 CCATGG C^CATGG NdeI 893 CATATG CA^TATG NspI 501 GCATGC [GA]CATG^[CT] PshBI 790 ATTAAT AT^TAAT PvuII 965 CAGCTG CAG^CTG RcaI 728 TCATGA T^CATGA RsaI 1305 GTAC GT^AC SmlI 514 CTCAAG C^T[CT][GA]AG SphI 501 GCATGC GCATG^C XcmI 1325 CCAAAGATACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 101 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 249 CCGC CCGC(-3/-1) 141 GCGG AcyI 3 GGCGTC G[GA]^CG[CT]C 336 GACGCC BcnI 145 CCGGG CC^[GC]GG 869 CCCGG BsaHI 3 GGCGTC G[GA]^CG[CT]C 336 GACGCC BsmAI 652 GAGAC GTCTC(1/5) 9 GTCTC BstAPI 873 GCACTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1015 GCACAGTTTGC BstXI 470 CCATGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 899 CCACTGCCATGG Cac8I 501 GCATGC GC[ACGT]^[ACGT]GC 1224 GCCAGC EarI 403 CTCTTC CTCTTC(1/4) 1270 CTCTTC EcoT14I 51 CCTAGG C^C[AT][AT]GG 905 CCATGG HapII 145 CCGG C^CGG 870 CCGG HgaI 336 GACGC GACGC(5/10) 4 GCGTC Hin1I 3 GGCGTC G[GA]^CG[CT]C 336 GACGCC HincII 481 GTTAAC GT[CT]^[GA]AC 970 GTCAAC HindII 481 GTTAAC GT[CT]^[GA]AC 970 GTCAAC HpaII 145 CCGG C^CGG 870 CCGG HphI 673 GGTGA GGTGA(8/7) 830 TCACC Ksp632I 403 CTCTTC CTCTTC(1/4) 1270 CTCTTC MslI 183 CATTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 900 CACTGCCATG MspI 145 CCGG C^CGG 870 CCGG MwoI 873 GCACTCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1015 GCACAGTTTGC NciI 145 CCGGG CC^[GC]GG 869 CCCGG PflMI 905 CCATGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1225 CCAGCCTTTGG SfaNI 860 GCATC GCATC(5/9) 1086 GCATC StyI 51 CCTAGG C^C[AT][AT]GG 905 CCATGG Van91I 905 CCATGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1225 CCAGCCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AluI AlwI BbvI BfaI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I