(Circular) (Six-base) MAPSORT of: pFN21AE5571.seq Check: 3778 from: 1 to: 6064 pFN21AE5571 6064 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2234 3407 1937 Size: 297 1173 4594 Fragments arranged by size: 4594 1173 297 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2193 2395 2978 3134 4127 5511 5779 1632 Size: 561 202 583 156 993 1384 268 1917 Fragments arranged by size: 1917 1384 993 583 561 268 202 156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 4963 5940 1203 Size: 3760 977 1327 Fragments arranged by size: 3760 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 4793 4793 Size: 6064 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 3807 5379 3807 Size: 1572 4492 ApaI G_GGCC'C Cuts at: 2211 2211 Size: 6064 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 3270 3373 3599 3751 5860 1493 Size: 1777 103 226 152 2109 1697 Fragments arranged by size: 2109 1777 1697 226 152 103 AseI AT'TA_AT Cuts at: 160 1032 2796 4618 160 Size: 872 1764 1822 1606 Fragments arranged by size: 1822 1764 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 3389 1870 Size: 73 1446 4545 Fragments arranged by size: 4545 1446 73 AvrII C'CTAG_G Cuts at: 2056 2056 Size: 6064 BamHI G'GATC_C Cuts at: 2106 3394 3761 2106 Size: 1288 367 4409 Fragments arranged by size: 4409 1288 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3242 3385 4840 618 Size: 325 217 627 1455 143 1455 1842 Fragments arranged by size: 1842 1455 1455 627 325 217 143 BanII G_rGCy'C Cuts at: 1391 2211 3383 1391 Size: 820 1172 4072 Fragments arranged by size: 4072 1172 820 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2938 928 Size: 971 1039 4054 Fragments arranged by size: 4054 1039 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4255 4289 1903 Size: 34 2318 34 3678 Fragments arranged by size: 3678 2318 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3956 5166 3956 Size: 1210 4854 BclI T'GATC_A Cuts at: 1548 1548 Size: 6064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4675 136 Size: 107 122 71 631 605 3003 1525 Fragments arranged by size: 3003 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 3481 3481 Size: 6064 Bme1580I G_kGCm'C Cuts at: 1790 2211 2875 4125 5281 1790 Size: 421 664 1250 1156 2573 Fragments arranged by size: 2573 1250 1156 664 421 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3275 3322 3795 Size: 416 53 83 399 24 1858 47 473 Cuts at: 3795 4753 442 Size: 958 1753 Fragments arranged by size: 1858 1753 958 473 416 399 83 53 47 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2606 4724 1239 Size: 1367 2118 2579 Fragments arranged by size: 2579 2118 1367 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2504 4189 5054 5352 2504 Size: 1685 865 298 3216 Fragments arranged by size: 3216 1685 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4727 882 Size: 617 3228 2219 Fragments arranged by size: 3228 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2565 3855 493 Size: 711 1361 1290 2702 Fragments arranged by size: 2702 1361 1290 711 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2342 3871 4253 Size: 53 83 186 936 476 333 1529 382 Cuts at: 4253 4888 275 Size: 635 1451 Fragments arranged by size: 1529 1451 936 635 476 382 333 186 83 53 BsaWI w'CCGG_w Cuts at: 4497 4895 5169 5316 5727 4497 Size: 398 274 147 411 4834 Fragments arranged by size: 4834 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2151 2181 2216 2246 1178 Size: 30 165 30 748 30 35 30 4996 Fragments arranged by size: 4996 748 165 35 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6064 BseYI C'CCAG_C Cuts at: 2812 5267 5763 2812 Size: 2455 496 3113 Fragments arranged by size: 3113 2455 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5491 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2150 3514 4275 5303 1540 Size: 275 186 149 1364 761 1028 2301 Fragments arranged by size: 2301 1364 1028 761 275 186 149 BsiHKAI G_wGCw'C Cuts at: 3177 4125 4210 5281 3177 Size: 948 85 1071 3960 Fragments arranged by size: 3960 1071 948 85 BsmI GAATG_Cn' Cuts at: 2939 3575 3668 2939 Size: 636 93 5335 Fragments arranged by size: 5335 636 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2019 2019 Size: 6064 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2875 3177 3383 4125 4210 5281 Size: 399 421 664 302 206 742 85 1071 Cuts at: 5281 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 664 421 399 302 206 85 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 2733 3953 5683 2733 Size: 1220 1730 3114 Fragments arranged by size: 3114 1730 1220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3419 844 Size: 2575 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3951 1394 Size: 429 2128 3507 Fragments arranged by size: 3507 2128 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2376 4559 4733 65 Size: 1183 1128 2183 174 1396 Fragments arranged by size: 2183 1396 1183 1128 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4708 4895 1700 Size: 110 2898 187 2869 Fragments arranged by size: 2898 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 3178 4124 5136 3178 Size: 946 1012 4106 Fragments arranged by size: 4106 1012 946 BstAPI GCAn_nnn'nTGC Cuts at: 794 2884 3026 794 Size: 2090 142 3832 Fragments arranged by size: 3832 2090 142 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6064 BstXI CCAn_nnnn'nTGG Cuts at: 2482 2911 2482 Size: 429 5635 BstYI r'GATC_y Cuts at: 1972 2106 3394 3761 4146 4163 5615 5701 5713 Size: 134 1288 367 385 17 1452 86 12 Cuts at: 5713 6059 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1288 385 367 346 134 86 17 12 BstZ17I GTA'TAC Cuts at: 2235 2235 Size: 6064 BtgI C'CryG_G Cuts at: 513 1065 2004 2910 513 Size: 552 939 906 3667 Fragments arranged by size: 3667 939 906 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5325 Fragments arranged by size: 5325 683 56 BtsI GCAGTG_nn' Cuts at: 2904 3361 3580 4374 4394 2904 Size: 457 219 794 20 4574 Fragments arranged by size: 4574 794 457 219 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2147 3511 4400 Size: 54 1425 50 275 18 317 1364 889 Cuts at: 4400 6029 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1364 889 317 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2147 3511 1537 Size: 275 335 1364 4090 Fragments arranged by size: 4090 1364 335 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2414 3281 3994 1353 Size: 1061 867 713 3423 Fragments arranged by size: 3423 1061 867 713 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2243 4651 5025 5171 387 Size: 1064 138 654 2408 374 146 1280 Fragments arranged by size: 2408 1280 1064 654 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2193 2395 2606 2978 3134 4127 4724 Size: 393 561 202 211 372 156 993 597 Cuts at: 4724 5511 5779 1239 Size: 787 268 1524 Fragments arranged by size: 1524 993 787 597 561 393 372 268 211 202 156 EcoRI G'AATT_C Cuts at: 3270 3373 3751 3270 Size: 103 378 5583 Fragments arranged by size: 5583 378 103 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2597 3144 2597 Size: 547 5517 FspI TGC'GCA Cuts at: 4570 4570 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 3188 5211 1678 Size: 1510 2023 2531 Fragments arranged by size: 2531 2023 1510 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2151 2183 2293 2325 2734 Size: 32 135 32 579 32 110 32 409 Cuts at: 2734 2766 3115 3147 3174 3206 4705 4737 4779 Size: 32 349 32 27 32 1499 32 42 Cuts at: 4779 4811 1373 Size: 32 2626 Fragments arranged by size: 2626 1499 579 409 349 135 110 42 32 32 32 32 32 32 32 32 32 27 HincII GTy'rAC Cuts at: 669 1657 1938 2488 2977 3408 3654 669 Size: 988 281 550 489 431 246 3079 Fragments arranged by size: 3079 988 550 489 431 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 2488 3654 2488 Size: 1166 4898 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2084 5178 5362 1305 Size: 779 3094 184 2007 Fragments arranged by size: 3094 2007 779 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4242 Fragments arranged by size: 4242 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2909 4022 4381 4540 518 Size: 648 1026 717 1113 359 159 2042 Fragments arranged by size: 2042 1113 1026 717 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2972 3480 4157 5305 5550 1606 Size: 1366 508 677 1148 245 2120 Fragments arranged by size: 2120 1366 1148 677 508 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2910 513 Size: 552 939 906 3667 Fragments arranged by size: 3667 939 906 552 NdeI CA'TA_TG Cuts at: 387 2899 387 Size: 2512 3552 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2510 3422 4967 1132 Size: 97 1281 912 1545 2229 Fragments arranged by size: 2229 1545 1281 912 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 4963 4963 Size: 6064 PflMI CCAn_nnn'nTGG Cuts at: 1108 2916 3236 1108 Size: 1808 320 3936 Fragments arranged by size: 3936 1808 320 PfoI T'CCnGG_A Cuts at: 2109 2109 Size: 6064 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6064 PsiI TTA'TAA Cuts at: 2690 3634 2690 Size: 944 5120 PspOMI G'GGCC_C Cuts at: 2207 2207 Size: 6064 PstI C_TGCA'G Cuts at: 830 3416 830 Size: 2586 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 PvuII CAG'CTG Cuts at: 2972 2972 Size: 6064 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SfcI C'TryA_G Cuts at: 826 1045 3412 4547 5228 5419 826 Size: 219 2367 1135 681 191 1471 Fragments arranged by size: 2367 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2519 4168 5069 5331 820 Size: 197 926 576 1649 901 262 1553 Fragments arranged by size: 1649 1553 926 901 576 262 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 2510 3422 1132 Size: 1378 912 3774 Fragments arranged by size: 3774 1378 912 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6064 StyI C'CwwG_G Cuts at: 513 1065 2004 2056 2910 3503 513 Size: 552 939 52 854 593 3074 Fragments arranged by size: 3074 939 854 593 552 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3052 4091 4108 4261 4446 4891 325 Size: 1255 1472 1039 17 153 185 445 1498 Fragments arranged by size: 1498 1472 1255 1039 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3309 4310 6022 Size: 274 80 33 51 1443 1332 1001 1712 Cuts at: 6022 96 Size: 138 Fragments arranged by size: 1712 1443 1332 1001 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6024 6056 6024 Size: 32 6032 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6064 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3337 3337 Size: 6064 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6064 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 4193 4193 Size: 6064 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI