(Circular) (Six-base) MAPSORT of: pFN21AE5553.seq Check: 6873 from: 1 to: 5983 pFN21AE5553 5983 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2880 3326 1937 Size: 943 446 4594 Fragments arranged by size: 4594 943 446 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 2692 2991 4046 5430 5698 1632 Size: 443 617 299 1055 1384 268 1917 Fragments arranged by size: 1917 1384 1055 617 443 299 268 AflII C'TTAA_G Cuts at: 820 1017 3047 820 Size: 197 2030 3756 Fragments arranged by size: 3756 2030 197 AflIII A'CryG_T Cuts at: 1203 2034 4882 5859 1203 Size: 831 2848 977 1327 Fragments arranged by size: 2848 1327 977 831 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 4712 4712 Size: 5983 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5983 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5983 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2021 2053 2060 2092 2021 Size: 32 7 32 5912 Fragments arranged by size: 5912 32 32 7 AlwNI CAG_nnn'CTG Cuts at: 3726 5298 3726 Size: 1572 4411 ApaLI G'TGCA_C Cuts at: 4040 5196 4040 Size: 1156 4827 ApoI r'AATT_y Cuts at: 1493 2617 3292 3518 3670 5779 1493 Size: 1124 675 226 152 2109 1697 Fragments arranged by size: 2109 1697 1124 675 226 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 3308 1870 Size: 73 1365 4545 Fragments arranged by size: 4545 1365 73 BamHI G'GATC_C Cuts at: 2757 3313 3680 2757 Size: 556 367 5060 Fragments arranged by size: 5060 556 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3304 4759 618 Size: 325 217 627 1517 1455 1842 Fragments arranged by size: 1842 1517 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2064 2073 3201 3302 1391 Size: 673 9 1128 101 4072 Fragments arranged by size: 4072 1128 673 101 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2612 2915 928 Size: 971 713 303 3996 Fragments arranged by size: 3996 971 713 303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4174 4208 1903 Size: 34 2237 34 3678 Fragments arranged by size: 3678 2237 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 3120 1548 Size: 1572 4411 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4594 136 Size: 107 122 71 631 605 2922 1525 Fragments arranged by size: 2922 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2175 5978 2175 Size: 3803 2180 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2784 4044 5200 1790 Size: 994 1260 1156 2573 Fragments arranged by size: 2573 1260 1156 994 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3714 4672 442 Size: 416 53 83 399 24 2297 958 1753 Fragments arranged by size: 2297 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 3077 4643 1239 Size: 875 963 1566 2579 Fragments arranged by size: 2579 1566 963 875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4108 4973 5271 4108 Size: 865 298 4820 Fragments arranged by size: 4820 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4646 882 Size: 617 3147 2219 Fragments arranged by size: 3147 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2035 2121 3774 493 Size: 711 831 86 1653 2702 Fragments arranged by size: 2702 1653 831 711 86 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2227 3790 4172 4807 Size: 53 83 186 936 694 1563 382 635 Cuts at: 4807 275 Size: 1451 Fragments arranged by size: 1563 1451 936 694 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4416 4814 5088 5235 5646 4416 Size: 398 274 147 411 4753 Fragments arranged by size: 4753 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2021 2051 2060 2090 1178 Size: 30 165 30 618 30 9 30 5071 Fragments arranged by size: 5071 618 165 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2391 2707 3188 1186 Size: 1205 316 481 3981 Fragments arranged by size: 3981 1205 481 316 BseYI C'CCAG_C Cuts at: 2256 5186 5682 2256 Size: 2930 496 2557 Fragments arranged by size: 2930 2557 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2761 1163 Size: 573 1025 4385 Fragments arranged by size: 4385 1025 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3433 4194 5222 1540 Size: 275 186 1432 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3201 4044 4129 5200 3201 Size: 843 85 1071 3984 Fragments arranged by size: 3984 1071 843 85 BsmI GAATG_Cn' Cuts at: 3256 3494 3587 3256 Size: 238 93 5652 Fragments arranged by size: 5652 238 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2073 2784 3201 3302 4044 4129 Size: 399 274 9 711 417 101 742 85 Cuts at: 4129 5200 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 711 417 399 274 101 85 9 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 3872 5602 3872 Size: 1730 4253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2376 3338 844 Size: 1532 962 3489 Fragments arranged by size: 3489 1532 962 BsrBI CCG'CTC Cuts at: 1394 1823 3870 1394 Size: 429 2047 3507 Fragments arranged by size: 3507 2047 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2124 2805 4478 4652 65 Size: 1183 876 681 1673 174 1396 Fragments arranged by size: 1673 1396 1183 876 681 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4627 4814 1700 Size: 110 2817 187 2869 Fragments arranged by size: 2869 2817 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 4043 5055 4043 Size: 1012 4971 BstAPI GCAn_nnn'nTGC Cuts at: 794 2480 794 Size: 1686 4297 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstYI r'GATC_y Cuts at: 1972 2175 2757 3313 3680 4065 4082 5534 5620 Size: 203 582 556 367 385 17 1452 86 Cuts at: 5620 5632 5978 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 582 556 385 367 346 203 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 2064 2811 2871 513 Size: 552 939 21 39 747 60 3625 Fragments arranged by size: 3625 939 747 552 60 39 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 1996 487 Size: 683 56 770 4474 Fragments arranged by size: 4474 770 683 56 BtsI GCAGTG_nn' Cuts at: 2282 3079 3499 4293 4313 2282 Size: 797 420 794 20 3952 Fragments arranged by size: 3952 797 794 420 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2534 2569 529 Size: 35 1970 35 3943 Fragments arranged by size: 3943 1970 35 35 DraI TTT'AAA Cuts at: 3287 3287 Size: 5983 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2114 4990 809 Size: 507 798 2876 1802 Fragments arranged by size: 2876 1802 798 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2022 3430 Size: 54 1425 50 275 18 177 15 1408 Cuts at: 3430 4319 5948 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1408 889 275 177 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3008 3913 1353 Size: 1655 905 3423 Fragments arranged by size: 3423 1655 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4570 4944 5090 387 Size: 1064 138 2981 374 146 1280 Fragments arranged by size: 2981 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3199 3199 Size: 5983 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2114 2692 2991 3077 4046 4643 Size: 393 443 39 578 299 86 969 597 Cuts at: 4643 5430 5698 1239 Size: 787 268 1524 Fragments arranged by size: 1524 969 787 597 578 443 393 299 268 86 39 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 FspI TGC'GCA Cuts at: 4489 4489 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 5130 1678 Size: 3452 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3017 3049 3061 3093 4624 Size: 32 135 32 1445 32 12 32 1531 Cuts at: 4624 4656 4698 4730 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1531 1445 135 42 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 2586 3582 2586 Size: 996 4987 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2926 5097 5281 1305 Size: 1621 2171 184 2007 Fragments arranged by size: 2171 2007 1621 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2024 10 Size: 54 1425 343 177 15 3969 Fragments arranged by size: 3969 1425 343 177 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2129 2810 3040 3941 4300 4459 518 Size: 648 963 681 230 901 359 159 2042 Fragments arranged by size: 2042 963 901 681 648 359 230 159 MspA1I CmG'CkG Cuts at: 1606 2377 2396 3399 4076 5224 5469 1606 Size: 771 19 1003 677 1148 245 2120 Fragments arranged by size: 2120 1148 1003 771 677 245 19 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4492 Fragments arranged by size: 4492 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 3341 4886 1132 Size: 97 2112 1545 2229 Fragments arranged by size: 2229 2112 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5983 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5983 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PmlI CAC'GTG Cuts at: 2121 2121 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2021 2053 2060 2092 4052 4084 5565 5597 2021 Size: 32 7 32 1960 32 1481 32 2407 Fragments arranged by size: 2407 1960 1481 32 32 32 32 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PstI C_TGCA'G Cuts at: 830 2289 3163 3335 830 Size: 1459 874 172 3478 Fragments arranged by size: 3478 1459 874 172 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 PvuII CAG'CTG Cuts at: 2396 2396 Size: 5983 SacI G_AGCT'C Cuts at: 3201 3201 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 2600 4231 5943 2600 Size: 1631 1712 2640 Fragments arranged by size: 2640 1712 1631 SfcI C'TryA_G Cuts at: 826 1045 2285 3159 3331 4466 5147 5338 826 Size: 219 1240 874 172 1135 681 191 1471 Fragments arranged by size: 1471 1240 1135 874 681 219 191 172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 3310 1872 Size: 1438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3047 4087 4988 5250 820 Size: 197 926 1104 1040 901 262 1553 Fragments arranged by size: 1553 1104 1040 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 3341 1132 Size: 2209 3774 SspI AAT'ATT Cuts at: 5 52 3064 3907 5 Size: 47 3012 843 2081 Fragments arranged by size: 3012 2081 843 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5983 StyI C'CwwG_G Cuts at: 513 1065 2004 3112 3202 3422 513 Size: 552 939 1108 90 220 3074 Fragments arranged by size: 3074 1108 939 552 220 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4010 4027 4180 4365 4810 325 Size: 1255 2430 17 153 185 445 1498 Fragments arranged by size: 2430 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2598 2640 4229 Size: 274 80 33 51 1443 621 42 1589 Cuts at: 4229 5941 96 Size: 1712 138 Fragments arranged by size: 1712 1589 1443 621 274 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5943 5975 5943 Size: 32 5951 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2017 2017 Size: 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 3308 1870 Size: 1438 4545 XmnI GAAnn'nnTTC Cuts at: 4112 4112 Size: 5983 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI