Restriction map for
pFN21AE5405
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AviII AvrII BalI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 707 GGTACC G^GTACC
AflIII 199 ACGTGT A^C[GA][CT]GT
AhdI 850 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 949 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 14 GGGCCC GGGCC^C
Asp718I 707 GGTACC G^GTACC
AspEI 850 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 547 GGCGCC GGCGC^C
BbrPI 198 CACGTG CAC^GTG
BbsI 847 GAAGAC GAAGAC(2/6)
BclI 773 TGATCA T^GATCA
BpuAI 847 GAAGAC GAAGAC(2/6)
BsiEI 7 CGACCG CG[GA][CT]^CG
BsmBI 802 GAGACG CGTCTC(1/5)
BsrFI 109 ACCGGC [GA]^CCGG[CT]
BstAPI 456 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 109 ACCGGC [GA]^CCGG[CT]
Eam1105I 850 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 698 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 935 CCATGG C^C[AT][AT]GG
FbaI 773 TGATCA T^GATCA
KasI 547 GGCGCC G^GCGCC
KpnI 707 GGTACC GGTAC^C
KspI 10 CCGCGG CCGC^GG
NarI 547 GGCGCC GG^CGCC
NcoI 935 CCATGG C^CATGG
PmaCI 198 CACGTG CAC^GTG
PmlI 198 CACGTG CAC^GTG
PstI 974 CTGCAG CTGCA^G
PvuII 796 CAGCTG CAG^CTG
SacI 304 GAGCTC GAGCT^C
SacII 10 CCGCGG CCGC^GG
SfcI 974 CTGCAG C^T[GA][CT]AG
SfoI 547 GGCGCC GGC^GCC
SgrAI 108 CACCGGCG C[GA]^CCGG[CT]G
SmaI 17 CCCGGG CCC^GGG
StyI 935 CCATGG C^C[AT][AT]GG
TfiI 941 GAATC G^A[AT]TC
XmaCI 17 CCCGGG C^CCGGG
XmaI 17 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 547 GGCGCC G[GA]^CG[CT]C
804 GACGCC
BbvCI 273 CCTCAGC CCTCAGC(-5/-2)
654 CCTCAGC
BglI 16 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
82 GCCTACCTGGC
BsaAI 198 CACGTG [CT]AC^GT[GA]
679 TACGTG
BsaHI 547 GGCGCC G[GA]^CG[CT]C
804 GACGCC
BseRI 271 CTCCTC GAGGAG(10/8)
553 CTCCTC
BsmAI 802 GAGAC GTCTC(1/5)
870 GAGAC
BsrI 450 ACTGG ACTGG(1/-1)
482 ACTGG
BssSI 307 CTCGTG CACGAG(-5/-1)
433 CTCGTG
DraII 876 GGGACCT [GA]G^G[ACGT]CC[CT]
930 GGGACCC
EcoO109I 876 GGGACCT [GA]G^G[ACGT]CC[CT]
930 GGGACCC
HgaI 123 GACGC GACGC(5/10)
804 GACGC
Hin1I 547 GGCGCC G[GA]^CG[CT]C
804 GACGCC
HphI 341 TCACC GGTGA(8/7)
650 TCACC
MaeII 199 ACGT A^CGT
680 ACGT
MslI 108 CACCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
285 CACCAGCATG
PpuMI 876 GGGACCT [GA]G^G[AT]CC[CT]
930 GGGACCC
SapI 570 GCTCTTC GCTCTTC(1/4)
594 GCTCTTC
TspRI 481 CACTG CA[GC]TG[ACGT][ACGT]^
713 CACTG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BamHI BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI XhoII