Restriction map for
pFN21AE5405
(Circular) (Six-base) MAPSORT of: pFN21AE5405.seq Check: 969 from: 1 to: 5701
pFN21AE5405 5701 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3044 1937
Size: 1107 4594
Acc65I G'GTAC_C
Cuts at: 2712 3022 2712
Size: 310 5391
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3764 5148 5416 1632
Size: 2132 1384 268 1917
Fragments arranged by size:
2132 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 2204 4600 5577 1203
Size: 1001 2396 977 1327
Fragments arranged by size:
2396 1327 1001 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 2860 4430 2860
Size: 1570 4131
AleI CACnn'nnGTG
Cuts at: 1166 2117 1166
Size: 951 4750
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5701
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2723 2755 2809 2841 2723
Size: 32 54 32 5583
Fragments arranged by size:
5583 54 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2959 3444 5016 2959
Size: 485 1572 3644
Fragments arranged by size:
3644 1572 485
ApaI G_GGCC'C
Cuts at: 2023 2023
Size: 5701
ApaLI G'TGCA_C
Cuts at: 3758 4914 3758
Size: 1156 4545
ApoI r'AATT_y
Cuts at: 1493 3010 3236 3388 5497 1493
Size: 1517 226 152 2109 1697
Fragments arranged by size:
2109 1697 1517 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2033 2563 3026 1870
Size: 73 79 11 530 463 4545
Fragments arranged by size:
4545 530 463 79 73 11
BamHI G'GATC_C
Cuts at: 2424 2518 2748 3031 3398 2424
Size: 94 230 283 367 4727
Fragments arranged by size:
4727 367 283 230 94
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2066 2111 2507 2552
Size: 325 217 627 272 7 45 396 45
Cuts at: 2552 2712 3022 4477 618
Size: 160 310 1455 1842
Fragments arranged by size:
1842 1455 627 396 325 310 272 217
160 45 45 7
BanII G_rGCy'C
Cuts at: 1391 2023 2313 2321 2375 2549 3020 1391
Size: 632 290 8 54 174 471 4072
Fragments arranged by size:
4072 632 471 290 174 54 8
BbeI G_GCGC'C
Cuts at: 2556 2556
Size: 5701
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2859 928
Size: 971 960 3770
Fragments arranged by size:
3770 971 960
BbvCI CC'TCA_GC
Cuts at: 2279 2660 2279
Size: 381 5320
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3892 3926 1903
Size: 34 1955 34 3678
Fragments arranged by size:
3678 1955 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 2778 1548
Size: 1230 4471
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 2093 4312
Size: 107 122 71 631 605 355 66 2219
Cuts at: 4312 136
Size: 1525
Fragments arranged by size:
2219 1525 631 605 355 122 107 71
66
BglII A'GATC_T
Cuts at: 5696 5696
Size: 5701
BlpI GC'TnA_GC
Cuts at: 3118 3118
Size: 5701
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2064 2114 2510 3762 4918 1790
Size: 233 41 50 396 1252 1156 2573
Fragments arranged by size:
2573 1252 1156 396 233 50 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2465 2497 3432
Size: 416 53 83 399 24 1048 32 935
Cuts at: 3432 4390 442
Size: 958 1753
Fragments arranged by size:
1753 1048 958 935 416 399 83 53
32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2291 2923 2291
Size: 632 5069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2140 2153 2641 2825 2893 4361 1239
Size: 901 13 488 184 68 1468 2579
Fragments arranged by size:
2579 1468 901 488 184 68 13
Bpu10I CC'TnA_GC
Cuts at: 2279 2369 2660 2279
Size: 90 291 5320
Fragments arranged by size:
5320 291 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 4691 4989 3826
Size: 865 298 4538
Fragments arranged by size:
4538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4364 882
Size: 617 2865 2219
Fragments arranged by size:
2865 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2205 2686 3492 493
Size: 711 1001 481 806 2702
Fragments arranged by size:
2702 1001 806 711 481
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2553 2810 3508 3890
Size: 53 83 186 936 1020 257 698 382
Cuts at: 3890 4525 275
Size: 635 1451
Fragments arranged by size:
1451 1020 936 698 635 382 257 186
83 53
BsaWI w'CCGG_w
Cuts at: 4134 4532 4806 4953 5364 4134
Size: 398 274 147 411 4471
Fragments arranged by size:
4471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2809 2839 2885 2915 1178
Size: 30 165 30 1406 30 46 30 3964
Fragments arranged by size:
3964 1406 165 46 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2549 1186
Size: 1081 282 4338
Fragments arranged by size:
4338 1081 282
BseYI C'CCAG_C
Cuts at: 2107 2304 2500 2799 4904 5400 2107
Size: 197 196 299 2105 496 2408
Fragments arranged by size:
2408 2105 496 299 197 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5128
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 3151 3912 4940 1540
Size: 275 186 14 1136 761 1028 2301
Fragments arranged by size:
2301 1136 1028 761 275 186 14
BsiHKAI G_wGCw'C
Cuts at: 2076 2313 2448 3762 3847 4918 2076
Size: 237 135 1314 85 1071 2859
Fragments arranged by size:
2859 1314 1071 237 135 85
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
BsmBI CGTCTCn'nnnn_
Cuts at: 2801 2801
Size: 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2064 2076 2114 2313 2321 2375
Size: 399 233 41 12 38 199 8 54
Cuts at: 2375 2448 2510 2549 3020 3762 3847 4918 1391
Size: 73 62 39 471 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 471 399 233 199 85
73 62 54 41 39 38 12 8
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 3590 5320 3590
Size: 1730 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3056 844
Size: 2212 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3588 1394
Size: 429 1765 3507
Fragments arranged by size:
3507 1765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4196 4370 65
Size: 1183 2948 174 1396
Fragments arranged by size:
2948 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2114 4345 4532 1700
Size: 110 304 2231 187 2869
Fragments arranged by size:
2869 2231 304 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5701
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5701
BssSI C'ACGA_G
Cuts at: 2312 2438 3761 4773 2312
Size: 126 1323 1012 3240
Fragments arranged by size:
3240 1323 1012 126
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2467 794
Size: 1673 4028
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5701
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2383 2500 2296
Size: 87 117 5497
Fragments arranged by size:
5497 117 87
BstYI r'GATC_y
Cuts at: 1972 2424 2518 2748 3031 3398 3783 3800 5252
Size: 452 94 230 283 367 385 17 1452
Cuts at: 5252 5338 5350 5696 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 452 385 367 346 283 230
94 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2015 2667 2940 513
Size: 552 950 652 273 3274
Fragments arranged by size:
3274 950 652 552 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4962
Fragments arranged by size:
4962 683 56
BtsI GCAGTG_nn'
Cuts at: 3217 4011 4031 3217
Size: 794 20 4887
Fragments arranged by size:
4887 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5666
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3148 4037 5666
Size: 54 1425 50 275 18 1318 889 1629
Cuts at: 5666 8
Size: 43
Fragments arranged by size:
1629 1425 1318 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3148 1537
Size: 275 1336 4090
Fragments arranged by size:
4090 1336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2402 2582 2606 3631 1353
Size: 1049 180 24 1025 3423
Fragments arranged by size:
3423 1049 1025 180 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4288 4662 4808 387
Size: 1064 138 2699 374 146 1280
Fragments arranged by size:
2699 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 2311
Size: 5701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2153 2641 2825 2893 3764 4361
Size: 393 508 13 488 184 68 871 597
Cuts at: 4361 5148 5416 1239
Size: 787 268 1524
Fragments arranged by size:
1524 871 787 597 508 488 393 268
184 68 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2707 2707
Size: 5701
EcoO109I rG'GnC_Cy
Cuts at: 2882 2936 2882
Size: 54 5647
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 5701
FspI TGC'GCA
Cuts at: 4207 4207
Size: 5701
HaeII r_GCGC'y
Cuts at: 1678 2108 2225 2305 2556 4848 1678
Size: 430 117 80 251 2292 2531
Fragments arranged by size:
2531 2292 430 251 117 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2846 2878 2885 2917 4342
Size: 32 135 32 1274 32 7 32 1425
Cuts at: 4342 4374 4416 4448 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1425 1274 135 42 32 32 32
32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3045 3291 669
Size: 988 281 1107 246 3079
Fragments arranged by size:
3079 1107 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KasI G'GCGC_C
Cuts at: 2552 2552
Size: 5701
KpnI G_GTAC'C
Cuts at: 2716 3026 2716
Size: 310 5391
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4815 4999 1305
Size: 3510 184 2007
Fragments arranged by size:
3510 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3879
Fragments arranged by size:
3879 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 2294 3659 4018 4177 518
Size: 648 951 177 1365 359 159 2042
Fragments arranged by size:
2042 1365 951 648 359 177 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2596 2803 3117 3794 4942 5187 1606
Size: 411 579 207 314 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 579 411 314 245 207
NarI GG'CG_CC
Cuts at: 2553 2553
Size: 5701
NcoI C'CATG_G
Cuts at: 513 1065 2940 513
Size: 552 1875 3274
Fragments arranged by size:
3274 1875 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 3148 3148
Size: 5701
NspI r_CATG'y
Cuts at: 1132 1229 3059 4604 1132
Size: 97 1830 1545 2229
Fragments arranged by size:
2229 1830 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5701
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5701
PfoI T'CCnGG_A
Cuts at: 2238 2238
Size: 5701
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PmlI CAC'GTG
Cuts at: 2205 2205
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2809 2841 3770 3802 5283 5315 2809
Size: 32 929 32 1481 32 3195
Fragments arranged by size:
3195 1481 929 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2882 2936 2882
Size: 54 5647
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 3271 3271
Size: 5701
PspOMI G'GGCC_C
Cuts at: 2019 2019
Size: 5701
PstI C_TGCA'G
Cuts at: 830 2983 3053 830
Size: 2153 70 3478
Fragments arranged by size:
3478 2153 70
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
PvuII CAG'CTG
Cuts at: 2803 2803
Size: 5701
SacI G_AGCT'C
Cuts at: 2313 2313
Size: 5701
SacII CC_GC'GG
Cuts at: 2018 2018
Size: 5701
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SanDI GG'GwC_CC
Cuts at: 2936 2936
Size: 5701
SapI GCTCTTCn'nnn_
Cuts at: 2582 2606 2582
Size: 24 5677
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SfcI C'TryA_G
Cuts at: 826 1045 2979 3049 4184 4865 5056 826
Size: 219 1934 70 1135 681 191 1471
Fragments arranged by size:
1934 1471 1135 681 219 191 70
SfoI GGC'GCC
Cuts at: 2554 2554
Size: 5701
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 2114 1700
Size: 414 5287
SmaI CCC'GGG
Cuts at: 1872 2024 3028 1872
Size: 152 1004 4545
Fragments arranged by size:
4545 1004 152
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3805 4706 4968 820
Size: 197 926 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 3059 1132
Size: 1927 3774
SrfI GCCC'GGGC
Cuts at: 2024 2024
Size: 5701
SspI AAT'ATT
Cuts at: 5 52 3625 5
Size: 47 3573 2081
Fragments arranged by size:
3573 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5701
StyI C'CwwG_G
Cuts at: 513 1065 2940 3140 513
Size: 552 1875 200 3074
Fragments arranged by size:
3074 1875 552 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3728 3745 3898 4083 4528 325
Size: 1255 2148 17 153 185 445 1498
Fragments arranged by size:
2148 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3947 5659 96
Size: 274 80 33 51 1443 1970 1712 138
Fragments arranged by size:
1970 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5661 5693 5661
Size: 32 5669
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 3037 3037
Size: 5701
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 2022 3026 1870
Size: 152 1004 4545
Fragments arranged by size:
4545 1004 152
XmnI GAAnn'nnTTC
Cuts at: 3830 3830
Size: 5701
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SexAI SfiI SwaI
XcmI