(Linear) (Six-base) MAPSORT of: KIEE5405.seq Check: 3451 from: 1 to: 996 KIEE5405.orf With 182 enzymes: SgfI * January 29, 2014 11:59 .. Acc65I G'GTAC_C Cuts at: 0 707 996 Size: 707 289 AflIII A'CryG_T Cuts at: 0 199 996 Size: 199 797 AhdI GACnn_n'nnGTC Cuts at: 0 855 996 Size: 855 141 AleI CACnn'nnGTG Cuts at: 0 112 996 Size: 112 884 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 718 750 804 836 996 Size: 718 32 54 32 160 Fragments arranged by size: 718 160 54 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 954 996 Size: 954 42 ApaI G_GGCC'C Cuts at: 0 18 996 Size: 18 978 AvaI C'yCGr_G Cuts at: 0 17 28 558 996 Size: 17 11 530 438 Fragments arranged by size: 530 438 17 11 BamHI G'GATC_C Cuts at: 0 419 513 743 996 Size: 419 94 230 253 Fragments arranged by size: 419 253 230 94 BanI G'GyrC_C Cuts at: 0 54 61 106 502 547 707 996 Size: 54 7 45 396 45 160 289 Fragments arranged by size: 396 289 160 54 45 45 7 BanII G_rGCy'C Cuts at: 0 18 308 316 370 544 996 Size: 18 290 8 54 174 452 Fragments arranged by size: 452 290 174 54 18 8 BbeI G_GCGC'C Cuts at: 0 551 996 Size: 551 445 BbsI GAAGACnn'nnnn_ Cuts at: 0 854 996 Size: 854 142 BbvCI CC'TCA_GC Cuts at: 0 274 655 996 Size: 274 381 341 Fragments arranged by size: 381 341 274 BclI T'GATC_A Cuts at: 0 773 996 Size: 773 223 BglI GCCn_nnn'nGGC Cuts at: 0 22 88 996 Size: 22 66 908 Fragments arranged by size: 908 66 22 Bme1580I G_kGCm'C Cuts at: 0 18 59 109 505 996 Size: 18 41 50 396 491 Fragments arranged by size: 491 396 50 41 18 BmrI ACTGGGnnnn_n' Cuts at: 0 460 492 996 Size: 460 32 504 Fragments arranged by size: 504 460 32 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 286 918 996 Size: 286 632 78 Fragments arranged by size: 632 286 78 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 135 148 636 820 888 996 Size: 135 13 488 184 68 108 Fragments arranged by size: 488 184 135 108 68 13 Bpu10I CC'TnA_GC Cuts at: 0 274 364 655 996 Size: 274 90 291 341 Fragments arranged by size: 341 291 274 90 BsaAI yAC'GTr Cuts at: 0 200 681 996 Size: 200 481 315 Fragments arranged by size: 481 315 200 BsaHI Gr'CG_yC Cuts at: 0 548 805 996 Size: 548 257 191 Fragments arranged by size: 548 257 191 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 804 834 880 910 996 Size: 804 30 46 30 86 Fragments arranged by size: 804 86 46 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 262 544 996 Size: 262 282 452 Fragments arranged by size: 452 282 262 BseYI C'CCAG_C Cuts at: 0 102 299 495 794 996 Size: 102 197 196 299 202 Fragments arranged by size: 299 202 197 196 102 BsiEI CG_ry'CG Cuts at: 0 10 996 Size: 10 986 BsiHKAI G_wGCw'C Cuts at: 0 71 308 443 996 Size: 71 237 135 553 Fragments arranged by size: 553 237 135 71 BsmBI CGTCTCn'nnnn_ Cuts at: 0 796 996 Size: 796 200 Bsp1286I G_dGCh'C Cuts at: 0 18 59 71 109 308 316 370 443 Size: 18 41 12 38 199 8 54 73 Cuts at: 443 505 544 996 Size: 62 39 452 Fragments arranged by size: 452 199 73 62 54 41 39 38 18 12 8 BsrFI r'CCGG_y Cuts at: 0 109 996 Size: 109 887 BssSI C'ACGA_G Cuts at: 0 307 433 996 Size: 307 126 563 Fragments arranged by size: 563 307 126 BstAPI GCAn_nnn'nTGC Cuts at: 0 462 996 Size: 462 534 BstXI CCAn_nnnn'nTGG Cuts at: 0 291 378 495 996 Size: 291 87 117 501 Fragments arranged by size: 501 291 117 87 BstYI r'GATC_y Cuts at: 0 419 513 743 996 Size: 419 94 230 253 Fragments arranged by size: 419 253 230 94 BtgI C'CryG_G Cuts at: 0 10 662 935 996 Size: 10 652 273 61 Fragments arranged by size: 652 273 61 10 EarI CTCTTCn'nnn_ Cuts at: 0 397 577 601 996 Size: 397 180 24 395 Fragments arranged by size: 397 395 180 24 EcoICRI GAG'CTC Cuts at: 0 306 996 Size: 306 690 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 135 148 636 820 888 996 Size: 135 13 488 184 68 108 Fragments arranged by size: 488 184 135 108 68 13 EcoNI CCTnn'n_nnAGG Cuts at: 0 702 996 Size: 702 294 EcoO109I rG'GnC_Cy Cuts at: 0 877 931 996 Size: 877 54 65 Fragments arranged by size: 877 65 54 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 867 996 Size: 867 129 HaeII r_GCGC'y Cuts at: 0 103 220 300 551 996 Size: 103 117 80 251 445 Fragments arranged by size: 445 251 117 103 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 841 873 880 912 996 Size: 841 32 7 32 84 Fragments arranged by size: 841 84 32 32 7 KasI G'GCGC_C Cuts at: 0 547 996 Size: 547 449 KpnI G_GTAC'C Cuts at: 0 711 996 Size: 711 285 MslI CAynn'nnrTG Cuts at: 0 112 289 996 Size: 112 177 707 Fragments arranged by size: 707 177 112 MspA1I CmG'CkG Cuts at: 0 12 591 798 996 Size: 12 579 207 198 Fragments arranged by size: 579 207 198 12 NarI GG'CG_CC Cuts at: 0 548 996 Size: 548 448 NcoI C'CATG_G Cuts at: 0 935 996 Size: 935 61 PfoI T'CCnGG_A Cuts at: 0 233 996 Size: 233 763 PmlI CAC'GTG Cuts at: 0 200 996 Size: 200 796 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 804 836 996 Size: 804 32 160 Fragments arranged by size: 804 160 32 PpuMI rG'GwC_Cy Cuts at: 0 877 931 996 Size: 877 54 65 Fragments arranged by size: 877 65 54 PspOMI G'GGCC_C Cuts at: 0 14 996 Size: 14 982 PstI C_TGCA'G Cuts at: 0 978 996 Size: 978 18 PvuII CAG'CTG Cuts at: 0 798 996 Size: 798 198 SacI G_AGCT'C Cuts at: 0 308 996 Size: 308 688 SacII CC_GC'GG Cuts at: 0 13 996 Size: 13 983 SanDI GG'GwC_CC Cuts at: 0 931 996 Size: 931 65 SapI GCTCTTCn'nnn_ Cuts at: 0 577 601 996 Size: 577 24 395 Fragments arranged by size: 577 395 24 SfcI C'TryA_G Cuts at: 0 974 996 Size: 974 22 SfoI GGC'GCC Cuts at: 0 549 996 Size: 549 447 SgrAI Cr'CCGG_yG Cuts at: 0 109 996 Size: 109 887 SmaI CCC'GGG Cuts at: 0 19 996 Size: 19 977 SrfI GCCC'GGGC Cuts at: 0 19 996 Size: 19 977 StyI C'CwwG_G Cuts at: 0 935 996 Size: 935 61 XmaI C'CCGG_G Cuts at: 0 17 996 Size: 17 979 Enzymes that do cut: Acc65I AflIII AhdI AleI AloI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BssSI BstAPI BstXI BstYI BtgI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI MslI MspA1I NarI NcoI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SfcI SfoI SgrAI SmaI SrfI StyI XmaI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AgeI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BpuEI BsaI BsaBI BsaWI BsgI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI