(Circular) (Six-base) MAPSORT of: pFN21AE5344.seq Check: 8895 from: 1 to: 5518 pFN21AE5344 5518 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3328 278 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2861 1937 Size: 924 4594 Acc65I G'GTAC_C Cuts at: 2839 2839 Size: 5518 AclI AA'CG_TT Cuts at: 1563 3645 4018 1563 Size: 2082 373 3063 Fragments arranged by size: 3063 2082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2184 2354 3581 4965 5233 1632 Size: 552 170 1227 1384 268 1917 Fragments arranged by size: 1917 1384 1227 552 268 170 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 4417 5394 1203 Size: 3214 977 1327 Fragments arranged by size: 3214 1327 977 AgeI A'CCGG_T Cuts at: 4349 4349 Size: 5518 AhdI GACnn_n'nnGTC Cuts at: 4247 4247 Size: 5518 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2490 1184 Size: 1306 4212 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2096 2128 2096 Size: 32 5486 AlwNI CAG_nnn'CTG Cuts at: 3261 4833 3261 Size: 1572 3946 ApaI G_GGCC'C Cuts at: 2130 2130 Size: 5518 ApaLI G'TGCA_C Cuts at: 3575 4731 3575 Size: 1156 4362 ApoI r'AATT_y Cuts at: 1493 2585 2827 3053 3205 5314 1493 Size: 1092 242 226 152 2109 1697 Fragments arranged by size: 2109 1697 1092 242 226 152 AseI AT'TA_AT Cuts at: 160 1032 2694 2706 4072 160 Size: 872 1662 12 1366 1606 Fragments arranged by size: 1662 1606 1366 872 12 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2118 2144 2189 2843 1870 Size: 73 70 105 26 45 654 4545 Fragments arranged by size: 4545 654 105 73 70 45 26 BamHI G'GATC_C Cuts at: 2848 3215 2848 Size: 367 5151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2839 4294 618 Size: 325 217 627 1052 1455 1842 Fragments arranged by size: 1842 1455 1052 627 325 217 BanII G_rGCy'C Cuts at: 1391 2087 2130 2837 1391 Size: 696 43 707 4072 Fragments arranged by size: 4072 707 696 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4547 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3709 3743 1903 Size: 34 50 34 1688 34 3678 Fragments arranged by size: 3678 1688 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4620 3410 Size: 1210 4308 BclI T'GATC_A Cuts at: 1548 2205 1548 Size: 657 4861 BfrBI ATG'CAT Cuts at: 2543 2543 Size: 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4129 136 Size: 107 122 71 631 605 2457 1525 Fragments arranged by size: 2457 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2275 5513 2275 Size: 3238 2280 BlpI GC'TnA_GC Cuts at: 2935 2935 Size: 5518 Bme1580I G_kGCm'C Cuts at: 1790 2130 3579 4735 1790 Size: 340 1449 1156 2573 Fragments arranged by size: 2573 1449 1156 340 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5518 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3249 4207 442 Size: 416 53 83 399 24 1832 958 1753 Fragments arranged by size: 1832 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 4178 1239 Size: 884 2055 2579 Fragments arranged by size: 2579 2055 884 Bpu10I CC'TnA_GC Cuts at: 2081 2081 Size: 5518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3643 4508 4806 3643 Size: 865 298 4355 Fragments arranged by size: 4355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4181 882 Size: 617 2682 2219 Fragments arranged by size: 2682 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3309 493 Size: 711 2105 2702 Fragments arranged by size: 2702 2105 711 BsaBI GATnn'nnATC Cuts at: 2460 5512 2460 Size: 3052 2466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3325 3707 4342 275 Size: 53 83 186 936 1792 382 635 1451 Fragments arranged by size: 1792 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2087 2787 3951 4349 4623 4770 5181 2087 Size: 700 1164 398 274 147 411 2424 Fragments arranged by size: 2424 1164 700 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 2417 2447 1178 Size: 30 165 30 693 30 291 30 4249 Fragments arranged by size: 4249 693 291 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 1186 Size: 934 4584 BseYI C'CCAG_C Cuts at: 2065 2679 2750 4721 5217 2065 Size: 614 71 1971 496 2366 Fragments arranged by size: 2366 1971 614 496 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2243 1163 Size: 573 507 4438 Fragments arranged by size: 4438 573 507 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2968 3729 4757 1540 Size: 275 186 967 761 1028 2301 Fragments arranged by size: 2301 1028 967 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2087 2253 3579 3664 4735 2087 Size: 166 1326 85 1071 2870 Fragments arranged by size: 2870 1326 1071 166 85 BsmI GAATG_Cn' Cuts at: 3029 3122 3029 Size: 93 5425 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2130 2253 2837 3579 3664 4735 Size: 399 297 43 123 584 742 85 1071 Cuts at: 4735 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 584 399 297 123 85 43 BspEI T'CCGG_A Cuts at: 2787 5181 2787 Size: 2394 3124 BspHI T'CATG_A Cuts at: 3407 5137 3407 Size: 1730 3788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2873 844 Size: 2029 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2230 3405 1394 Size: 429 407 1175 3507 Fragments arranged by size: 3507 1175 429 407 BsrDI GCAATG_nn' Cuts at: 65 1248 2436 4013 4187 65 Size: 1183 1188 1577 174 1396 Fragments arranged by size: 1577 1396 1188 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2073 4162 4349 1700 Size: 110 263 2089 187 2869 Fragments arranged by size: 2869 2089 263 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5518 BssSI C'ACGA_G Cuts at: 3578 4590 3578 Size: 1012 4506 BstAPI GCAn_nnn'nTGC Cuts at: 794 2257 794 Size: 1463 4055 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5518 BstXI CCAn_nnnn'nTGG Cuts at: 2811 2811 Size: 5518 BstYI r'GATC_y Cuts at: 1972 2275 2848 3215 3600 3617 5069 5155 5167 Size: 303 573 367 385 17 1452 86 12 Cuts at: 5167 5513 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 573 385 367 346 303 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2772 513 Size: 552 1707 3259 Fragments arranged by size: 3259 1707 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4779 Fragments arranged by size: 4779 683 56 BtsI GCAGTG_nn' Cuts at: 3034 3828 3848 3034 Size: 794 20 4704 Fragments arranged by size: 4704 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5483 DraI TTT'AAA Cuts at: 2482 2822 2482 Size: 340 5178 DraIII CAC_nnn'GTG Cuts at: 2310 2310 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2783 4525 809 Size: 507 1467 1742 1802 Fragments arranged by size: 1802 1742 1467 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2965 3854 5483 Size: 54 1425 50 275 18 1135 889 1629 Cuts at: 5483 8 Size: 43 Fragments arranged by size: 1629 1425 1135 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2965 1537 Size: 275 1153 4090 Fragments arranged by size: 4090 1153 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3448 1353 Size: 2095 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2085 2085 Size: 5518 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2184 2354 3581 4178 4965 5233 Size: 393 491 61 170 1227 597 787 268 Cuts at: 5233 1239 Size: 1524 Fragments arranged by size: 1524 1227 787 597 491 393 268 170 61 EcoO109I rG'GnC_Cy Cuts at: 2127 2127 Size: 5518 EcoRI G'AATT_C Cuts at: 2827 3205 2827 Size: 378 5140 FspI TGC'GCA Cuts at: 4024 4024 Size: 5518 HaeII r_GCGC'y Cuts at: 1678 2031 2152 4665 1678 Size: 353 121 2513 2531 Fragments arranged by size: 2531 2513 353 121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2782 2814 4159 4191 4233 Size: 32 135 32 1210 32 1345 32 42 Cuts at: 4233 4265 1373 Size: 32 2626 Fragments arranged by size: 2626 1345 1210 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2862 3108 669 Size: 988 281 924 246 3079 Fragments arranged by size: 3079 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5518 HpaI GTT'AAC Cuts at: 3108 3108 Size: 5518 KpnI G_GTAC'C Cuts at: 2843 2843 Size: 5518 MfeI C'AATT_G Cuts at: 3117 3117 Size: 5518 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2395 2828 4632 4816 1305 Size: 1090 433 1804 184 2007 Fragments arranged by size: 2007 1804 1090 433 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3696 Fragments arranged by size: 3696 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3476 3835 3994 518 Size: 648 2310 359 159 2042 Fragments arranged by size: 2310 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2101 2332 2934 3611 4759 5004 1606 Size: 495 231 602 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 602 495 245 231 NaeI GCC'GGC Cuts at: 2075 2075 Size: 5518 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4966 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NgoMIV G'CCGG_C Cuts at: 2073 2073 Size: 5518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2965 2965 Size: 5518 NsiI A_TGCA'T Cuts at: 2545 2545 Size: 5518 NspI r_CATG'y Cuts at: 1132 1229 2543 2765 2876 4421 1132 Size: 97 1314 222 111 1545 2229 Fragments arranged by size: 2229 1545 1314 222 111 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5518 PciI A'CATG_T Cuts at: 4417 4417 Size: 5518 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5518 PfoI T'CCnGG_A Cuts at: 2394 2394 Size: 5518 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2096 2128 3587 3619 5100 5132 2096 Size: 32 1459 32 1481 32 2482 Fragments arranged by size: 2482 1481 1459 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5518 PsiI TTA'TAA Cuts at: 3088 3088 Size: 5518 PspOMI G'GGCC_C Cuts at: 2126 2126 Size: 5518 PspXI vC'TCGA_Gb Cuts at: 2144 2144 Size: 5518 PstI C_TGCA'G Cuts at: 830 2410 2870 830 Size: 1580 460 3478 Fragments arranged by size: 3478 1580 460 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5518 PvuII CAG'CTG Cuts at: 2332 2332 Size: 5518 SacI G_AGCT'C Cuts at: 2087 2087 Size: 5518 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SbfI CC_TGCA'GG Cuts at: 2870 2870 Size: 5518 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SfcI C'TryA_G Cuts at: 826 1045 2406 2572 2664 2866 4001 4682 4873 Size: 219 1361 166 92 202 1135 681 191 Cuts at: 4873 826 Size: 1471 Fragments arranged by size: 1471 1361 1135 681 219 202 191 166 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5518 SmaI CCC'GGG Cuts at: 1872 2015 2120 2845 1872 Size: 143 105 725 4545 Fragments arranged by size: 4545 725 143 105 SmlI C'TyrA_G Cuts at: 820 1017 1943 2144 3622 4523 4785 820 Size: 197 926 201 1478 901 262 1553 Fragments arranged by size: 1553 1478 926 901 262 201 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2876 1132 Size: 1744 3774 SspI AAT'ATT Cuts at: 5 52 2510 2673 3442 5 Size: 47 2458 163 769 2081 Fragments arranged by size: 2458 2081 769 163 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5518 StyI C'CwwG_G Cuts at: 513 1065 2957 513 Size: 552 1892 3074 Fragments arranged by size: 3074 1892 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3545 3562 3715 3900 4345 325 Size: 1255 1965 17 153 185 445 1498 Fragments arranged by size: 1965 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2608 3764 5476 Size: 274 80 33 51 1443 631 1156 1712 Cuts at: 5476 96 Size: 138 Fragments arranged by size: 1712 1443 1156 631 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5478 5510 5478 Size: 32 5486 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5518 XbaI T'CTAG_A Cuts at: 2854 2854 Size: 5518 XhoI C'TCGA_G Cuts at: 1943 2144 1943 Size: 201 5317 XmaI C'CCGG_G Cuts at: 1870 2013 2118 2843 1870 Size: 143 105 725 4545 Fragments arranged by size: 4545 725 143 105 XmnI GAAnn'nnTTC Cuts at: 2511 3647 2511 Size: 1136 4382 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3326 276 Size: 53 83 186 936 1792 2468 Fragments arranged by size: 2468 1792 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI