################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII BalI BamHI BanII BbeI BbsI BbvCI BcgI BcnI BglI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BspEI BspLU11I BspMI BsrBI BssHII Bst1107I BstBI BstUI BstZ17I CelII CfoI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcsI 291 GAATTT [GA]^AATT[CT] AflII 154 CTTAAG C^TTAAG ApoI 291 GAATTT [GA]^AATT[CT] AvaI 716 CCCGAG C^[CT]CG[GA]G AvrII 564 CCTAGG C^CTAGG BanI 599 GGCACC G^G[CT][GA]CC BbrPI 625 CACGTG CAC^GTG BciVI 728 GTATCC GTATCC(6/5) BclI 581 TGATCA T^GATCA BfaI 565 CTAG C^TAG BfrI 154 CTTAAG C^TTAAG BglII 383 AGATCT A^GATCT BlnI 564 CCTAGG C^CTAGG BmyI 393 GGGCAC G[AGT]GC[ACT]^C BpmI 551 CTGGAG CTGGAG(16/14) BsaAI 625 CACGTG [CT]AC^GT[GA] BsoBI 716 CCCGAG C^[CT]CG[GA]G Bsp1286I 393 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 330 TGTACA T^GTACA BspDI 214 ATCGAT AT^CGAT BspHI 423 TCATGA T^CATGA BsrDI 741 CATTGC GCAATG(2/0) BsrGI 330 TGTACA T^GTACA BssKI 604 CCTGG ^CC[ACGT]GG BssSI 5 CTCGTG CACGAG(-5/-1) BstAPI 48 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 532 GGTAACC G^GT[ACGT]ACC BstNI 604 CCTGG CC^[AT]GG BstPI 532 GGTAACC G^GT[ACGT]ACC BstXI 43 CCAAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 214 ATCGAT AT^CGAT DsaI 700 CCATGG C^C[GA][CT]GG EcoNI 456 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 532 GGTAACC G^GT[ACGT]ACC EcoRII 604 CCTGG ^CC[AT]GG FbaI 581 TGATCA T^GATCA HaeIII 354 GGCC GG^CC MaeI 565 CTAG C^TAG MslI 459 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 199 CAGCGG C[AC]G^C[GT]G MvaI 604 CCTGG CC^[AT]GG NcoI 700 CCATGG C^CATGG NspI 194 GCATGC [GA]CATG^[CT] PleI 420 GACTC GAGTC(4/5) PmaCI 625 CACGTG CAC^GTG PmlI 625 CACGTG CAC^GTG RcaI 423 TCATGA T^CATGA ScrFI 604 CCTGG CC^[ACGT]GG SmlI 154 CTTAAG C^T[CT][GA]AG SphI 194 GCATGC GCATG^C SspBI 330 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 331 GTAC GT^AC 360 GTAC AgeI 70 ACCGGT A^CCGGT 97 ACCGGT AluI 39 AGCT AG^CT 649 AGCT AlwI 451 GATCC GGATC(4/5) 607 GGATC AocI 141 CCTGAGG CC^T[ACGT]AGG 346 CCTGAGG BsaWI 70 ACCGGT [AT]^CCGG[AT] 97 ACCGGT BseRI 83 CTCCTC GAGGAG(10/8) 349 GAGGAG BsmFI 11 GTCCC GGGAC(10/14) 101 GTCCC BsrFI 70 ACCGGT [GA]^CCGG[CT] 97 ACCGGT BstYI 383 AGATCT [GA]^GATC[CT] 607 GGATCT Bsu36I 141 CCTGAGG CC^T[ACGT]AGG 346 CCTGAGG Cac8I 194 GCATGC GC[ACGT]^[ACGT]GC 430 GCCCGC Cfr10I 70 ACCGGT [GA]^CCGG[CT] 97 ACCGGT DdeI 142 CTGAG C^T[ACGT]AG 347 CTGAG EarI 710 CTCTTC CTCTTC(1/4) 748 GAAGAG Eco81I 141 CCTGAGG CC^T[ACGT]AGG 346 CCTGAGG FauI 431 CCCGC CCCGC(4/6) 30 GCGGG HapII 71 CCGG C^CGG 98 CCGG HpaII 71 CCGG C^CGG 98 CCGG Ksp632I 710 CTCTTC CTCTTC(1/4) 748 GAAGAG MaeII 617 ACGT A^CGT 626 ACGT MflI 383 AGATCT [GA]^GATC[CT] 607 GGATCT MseI 155 TTAA T^TAA 209 TTAA MspI 71 CCGG C^CGG 98 CCGG PinAI 70 ACCGGT A^CCGGT 97 ACCGGT RsaI 331 GTAC GT^AC 360 GTAC TfiI 254 GATTC G^A[AT]TC 404 GAATC Tru9I 155 TTAA T^TAA 209 TTAA Tsp45I 418 GTGAC ^GT[GC]AC 547 GTGAC TspRI 528 CACTG CA[GC]TG[ACGT][ACGT]^ 783 CAGTG XhoII 383 AGATCT [GA]^GATC[CT] 607 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AvaII BbvI BsaJI BsiYI BslI BsmAI BsrI BstF5I Cfr13I DpnII EcoT14I Fnu4HI FokI HinfI HphI ItaI MaeIII MboI MboII MnlI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I SfaNI StyI TaqI TseI Tsp509I TthHB8I