(Circular) (Six-base) MAPSORT of: pFN21AE5340.seq Check: 4287 from: 1 to: 5494 pFN21AE5340 5494 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3304 278 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2837 1937 Size: 900 4594 Acc65I G'GTAC_C Cuts at: 2815 2815 Size: 5494 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 2772 3557 4941 5209 1632 Size: 419 721 785 1384 268 1917 Fragments arranged by size: 1917 1384 785 721 419 268 AflII C'TTAA_G Cuts at: 820 1017 2159 820 Size: 197 1142 4155 Fragments arranged by size: 4155 1142 197 AflIII A'CryG_T Cuts at: 1203 4393 5370 1203 Size: 3190 977 1327 Fragments arranged by size: 3190 1327 977 AgeI A'CCGG_T Cuts at: 2075 2102 4325 2075 Size: 27 2223 3244 Fragments arranged by size: 3244 2223 27 AhdI GACnn_n'nnGTC Cuts at: 4223 4223 Size: 5494 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5494 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5494 AlwNI CAG_nnn'CTG Cuts at: 3237 4809 3237 Size: 1572 3922 ApaLI G'TGCA_C Cuts at: 3551 4707 3551 Size: 1156 4338 ApoI r'AATT_y Cuts at: 1493 2296 2803 3029 3181 5290 1493 Size: 803 507 226 152 2109 1697 Fragments arranged by size: 2109 1697 803 507 226 152 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2721 2819 1870 Size: 73 778 98 4545 Fragments arranged by size: 4545 778 98 73 AvrII C'CTAG_G Cuts at: 2569 2569 Size: 5494 BamHI G'GATC_C Cuts at: 2824 3191 2824 Size: 367 5127 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2604 2815 4270 618 Size: 325 217 627 817 211 1455 1842 Fragments arranged by size: 1842 1455 817 627 325 217 211 BanII G_rGCy'C Cuts at: 1391 2813 1391 Size: 1422 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4523 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3685 3719 1903 Size: 34 1748 34 3678 Fragments arranged by size: 3678 1748 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2744 3386 4596 2744 Size: 642 1210 3642 Fragments arranged by size: 3642 1210 642 BclI T'GATC_A Cuts at: 1548 2586 1548 Size: 1038 4456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4105 136 Size: 107 122 71 631 605 2433 1525 Fragments arranged by size: 2433 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2388 5489 2388 Size: 3101 2393 BlpI GC'TnA_GC Cuts at: 2911 2911 Size: 5494 Bme1580I G_kGCm'C Cuts at: 1790 2402 3555 4711 1790 Size: 612 1153 1156 2573 Fragments arranged by size: 2573 1156 1153 612 BmgBI CAC'GTC Cuts at: 1318 2623 1318 Size: 1305 4189 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3225 4183 442 Size: 416 53 83 399 24 1808 958 1753 Fragments arranged by size: 1808 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2144 2144 Size: 5494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2577 4154 1239 Size: 1338 1577 2579 Fragments arranged by size: 2579 1577 1338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3619 4484 4782 3619 Size: 865 298 4331 Fragments arranged by size: 4331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4157 882 Size: 617 2658 2219 Fragments arranged by size: 2658 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2632 3285 493 Size: 711 1428 653 2702 Fragments arranged by size: 2702 1428 711 653 BsaBI GATnn'nnATC Cuts at: 5488 5488 Size: 5494 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3301 3683 4318 275 Size: 53 83 186 936 1768 382 635 1451 Fragments arranged by size: 1768 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2075 2102 3927 4325 4599 4746 5157 2075 Size: 27 1825 398 274 147 411 2412 Fragments arranged by size: 2412 1825 411 398 274 147 27 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2169 2199 2348 2378 1178 Size: 30 165 30 766 30 149 30 4294 Fragments arranged by size: 4294 766 165 149 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2079 2369 1186 Size: 893 290 4311 Fragments arranged by size: 4311 893 290 BseYI C'CCAG_C Cuts at: 4697 5193 4697 Size: 496 4998 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4921 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2944 3705 4733 1540 Size: 275 186 943 761 1028 2301 Fragments arranged by size: 2301 1028 943 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3555 3640 4711 3555 Size: 85 1071 4338 Fragments arranged by size: 4338 1071 85 BsmI GAATG_Cn' Cuts at: 3005 3098 3005 Size: 93 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2402 2813 3555 3640 4711 1391 Size: 399 612 411 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 612 411 399 85 BspEI T'CCGG_A Cuts at: 5157 5157 Size: 5494 BspHI T'CATG_A Cuts at: 2428 3383 5113 2428 Size: 955 1730 2809 Fragments arranged by size: 2809 1730 955 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2849 844 Size: 2005 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3381 1394 Size: 429 1558 3507 Fragments arranged by size: 3507 1558 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2745 3989 4163 65 Size: 1183 1497 1244 174 1396 Fragments arranged by size: 1497 1396 1244 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2075 2102 4138 4325 1700 Size: 110 265 27 2036 187 2869 Fragments arranged by size: 2869 2036 265 187 110 27 BsrGI T'GTAC_A Cuts at: 96 2335 96 Size: 2239 3255 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 2010 3554 4566 2010 Size: 1544 1012 2938 Fragments arranged by size: 2938 1544 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2059 794 Size: 1265 4229 BstBI TT'CG_AA Cuts at: 4298 4298 Size: 5494 BstEII G'GTnAC_C Cuts at: 1184 2537 1184 Size: 1353 4141 BstXI CCAn_nnnn'nTGG Cuts at: 2055 2055 Size: 5494 BstYI r'GATC_y Cuts at: 1972 2388 2612 2824 3191 3576 3593 5045 5131 Size: 416 224 212 367 385 17 1452 86 Cuts at: 5131 5143 5489 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 416 385 367 346 224 212 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2147 2352 2147 Size: 205 5289 BtgI C'CryG_G Cuts at: 513 1065 2004 2705 513 Size: 552 939 701 3302 Fragments arranged by size: 3302 939 701 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4755 Fragments arranged by size: 4755 683 56 BtsI GCAGTG_nn' Cuts at: 2794 3010 3804 3824 2794 Size: 216 794 20 4464 Fragments arranged by size: 4464 794 216 20 ClaI AT'CG_AT Cuts at: 1554 2220 1554 Size: 666 4828 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5459 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4501 809 Size: 507 3185 1802 Fragments arranged by size: 3185 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2941 3830 5459 Size: 54 1425 50 275 18 1111 889 1629 Cuts at: 5459 8 Size: 43 Fragments arranged by size: 1629 1425 1111 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2941 1537 Size: 275 1129 4090 Fragments arranged by size: 4090 1129 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2721 2748 3424 1353 Size: 1368 27 676 3423 Fragments arranged by size: 3423 1368 676 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2726 4081 4455 4601 387 Size: 1064 138 1137 1355 374 146 1280 Fragments arranged by size: 1355 1280 1137 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2577 2772 3557 4154 4941 5209 Size: 393 419 526 195 785 597 787 268 Cuts at: 5209 1239 Size: 1524 Fragments arranged by size: 1524 787 785 597 526 419 393 268 195 EcoNI CCTnn'n_nnAGG Cuts at: 2465 2465 Size: 5494 EcoRI G'AATT_C Cuts at: 2803 3181 2803 Size: 378 5116 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2344 2344 Size: 5494 FspI TGC'GCA Cuts at: 4000 4000 Size: 5494 HaeII r_GCGC'y Cuts at: 1678 4641 1678 Size: 2963 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2110 2142 4135 4167 4209 Size: 32 135 32 538 32 1993 32 42 Cuts at: 4209 4241 1373 Size: 32 2626 Fragments arranged by size: 2626 1993 538 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2838 3084 669 Size: 988 281 900 246 3079 Fragments arranged by size: 3079 988 900 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5494 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KpnI G_GTAC'C Cuts at: 2819 2819 Size: 5494 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2697 4608 4792 1305 Size: 1392 1911 184 2007 Fragments arranged by size: 2007 1911 1392 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3672 Fragments arranged by size: 3672 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2468 3452 3811 3970 518 Size: 648 1302 984 359 159 2042 Fragments arranged by size: 2042 1302 984 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2206 2910 3587 4735 4980 1606 Size: 600 704 677 1148 245 2120 Fragments arranged by size: 2120 1148 704 677 600 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2705 513 Size: 552 939 701 3302 Fragments arranged by size: 3302 939 701 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2203 2852 4397 1132 Size: 97 974 649 1545 2229 Fragments arranged by size: 2229 1545 974 649 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5494 PciI A'CATG_T Cuts at: 4393 4393 Size: 5494 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PmlI CAC'GTG Cuts at: 2632 2632 Size: 5494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5076 5108 3563 Size: 32 1481 32 3949 Fragments arranged by size: 3949 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5494 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PstI C_TGCA'G Cuts at: 830 2846 830 Size: 2016 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SbfI CC_TGCA'GG Cuts at: 2846 2846 Size: 5494 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SfcI C'TryA_G Cuts at: 826 1045 2842 3977 4658 4849 826 Size: 219 1797 1135 681 191 1471 Fragments arranged by size: 1797 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2821 1872 Size: 949 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 3598 4499 4761 820 Size: 197 926 216 1439 901 262 1553 Fragments arranged by size: 1553 1439 926 901 262 216 197 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2203 2852 1132 Size: 1071 649 3774 Fragments arranged by size: 3774 1071 649 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5494 StyI C'CwwG_G Cuts at: 513 1065 2004 2319 2569 2705 2933 513 Size: 552 939 315 250 136 228 3074 Fragments arranged by size: 3074 939 552 315 250 228 136 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3521 3538 3691 3876 4321 325 Size: 1255 1941 17 153 185 445 1498 Fragments arranged by size: 1941 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2335 2364 3740 Size: 274 80 33 51 1443 358 29 1376 Cuts at: 3740 5452 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1376 358 274 138 80 51 33 29 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5454 5486 5454 Size: 32 5462 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5494 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5494 XmaI C'CCGG_G Cuts at: 1870 2819 1870 Size: 949 4545 XmnI GAAnn'nnTTC Cuts at: 3623 3623 Size: 5494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3302 276 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI