Restriction map for
pFN21AE5317
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AvaII 661 GGTCC G^G[AT]CC
BanII 393 GGGCTC G[GA]GC[CT]^C
BbrPI 698 CACGTG CAC^GTG
BcgI 341 GCAATGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 414 CCGGG CC^[GC]GG
BfaI 332 CTAG C^TAG
BglI 111 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 698 CACGTG [CT]AC^GT[GA]
BsgI 242 CTGCAC GTGCAG(16/14)
BsmFI 379 GTCCC GGGAC(10/14)
BspMI 658 GCAGGT ACCTGC(4/8)
BsrBI 678 CCGCTC CCGCTC(-3/-3)
Bst1107I 636 GTATAC GTA^TAC
BstAPI 215 GCAAACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 636 GTATAC GTA^TAC
DraII 660 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 16 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 428 CCACGG C^C[GA][CT]GG
EcoNI 136 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 660 AGGTCCT [GA]G^G[ACGT]CC[CT]
HincII 575 GTCAAC GT[CT]^[GA]AC
HindII 575 GTCAAC GT[CT]^[GA]AC
MaeI 332 CTAG C^TAG
MaeII 699 ACGT A^CGT
NciI 414 CCGGG CC^[GC]GG
PmaCI 698 CACGTG CAC^GTG
PmlI 698 CACGTG CAC^GTG
PpuMI 660 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 356 CTGCAG CTGCA^G
PvuII 476 CAGCTG CAG^CTG
SexAI 73 ACCAGGT A^CC[AT]GGT
Tsp45I 229 GTCAC ^GT[GC]AC
TspRI 65 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 78 GTCTAC GT^[AC][GT]AC
636 GTATAC
AcsI 692 GAATTC [GA]^AATT[CT]
778 GAATTC
AlwI 3 GATCC GGATC(4/5)
420 GATCC
ApoI 692 GAATTC [GA]^AATT[CT]
778 GAATTC
BmyI 182 GTGCCC G[AGT]GC[ACT]^C
393 GGGCTC
BpmI 365 CTCCAG CTGGAG(16/14)
187 CTGGAG
BseRI 323 CTCCTC GAGGAG(10/8)
190 GAGGAG
Bsp1286I 182 GTGCCC G[AGT]GC[ACT]^C
393 GGGCTC
BsrDI 224 GCAATG GCAATG(2/0)
341 GCAATG
Cfr13I 584 GGGCC G^G[ACGT]CC
661 GGTCC
EarI 151 GAAGAG CTCTTC(1/4)
769 GAAGAG
EcoRI 692 GAATTC G^AATTC
778 GAATTC
FauI 677 CCCGC CCCGC(4/6)
582 GCGGG
HapII 414 CCGG C^CGG
783 CCGG
HpaII 414 CCGG C^CGG
783 CCGG
Ksp632I 151 GAAGAG CTCTTC(1/4)
769 GAAGAG
MseI 401 TTAA T^TAA
608 TTAA
MspA1I 476 CAGCTG C[AC]G^C[GT]G
829 CAGCGG
MspI 414 CCGG C^CGG
783 CCGG
NlaIV 85 GGAACC GG[ACGT]^[ACGT]CC
394 GGCTCC
Sau96I 584 GGGCC G^G[ACGT]CC
661 GGTCC
SfaNI 457 GATGC GCATC(5/9)
615 GATGC
SfcI 80 CTACAG C^T[GA][CT]AG
356 CTGCAG
TaqI 160 TCGA T^CGA
350 TCGA
Tru9I 401 TTAA T^TAA
608 TTAA
TthHB8I 160 TCGA T^CGA
350 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI BalI BbvI
BsaJI BsiYI BslI BsrI BssKI
BstNI Cac8I DdeI DpnII EaeI
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MluNI
MnlI MscI MvaI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI TfiI
TseI Tsp509I