(Circular) (Six-base) MAPSORT of: pFN21AE5317.seq Check: 281 from: 1 to: 5572 pFN21AE5317 5572 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3382 278 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2084 2642 2915 1937 Size: 147 558 273 4594 Fragments arranged by size: 4594 558 273 147 Acc65I G'GTAC_C Cuts at: 2893 2893 Size: 5572 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2455 3635 5019 5287 1632 Size: 823 1180 1384 268 1917 Fragments arranged by size: 1917 1384 1180 823 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 4471 5448 1203 Size: 3268 977 1327 Fragments arranged by size: 3268 1327 977 AgeI A'CCGG_T Cuts at: 4403 4403 Size: 5572 AhdI GACnn_n'nnGTC Cuts at: 4301 4301 Size: 5572 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5572 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2202 1184 Size: 1018 4554 AlwNI CAG_nnn'CTG Cuts at: 3315 4887 3315 Size: 1572 4000 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2697 2783 2881 3107 3259 5368 1493 Size: 1204 86 98 226 152 2109 1697 Fragments arranged by size: 2109 1697 1204 226 152 98 86 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2897 1870 Size: 73 954 4545 Fragments arranged by size: 4545 954 73 BamHI G'GATC_C Cuts at: 2902 3269 2902 Size: 367 5205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2893 4348 618 Size: 325 217 627 1106 1455 1842 Fragments arranged by size: 1842 1455 1106 627 325 217 BanII G_rGCy'C Cuts at: 1391 2402 2891 1391 Size: 1011 489 4072 Fragments arranged by size: 4072 1011 489 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4601 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2335 2369 3763 3797 1903 Size: 34 398 34 1394 34 3678 Fragments arranged by size: 3678 1394 398 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3464 4674 3464 Size: 1210 4362 BclI T'GATC_A Cuts at: 1548 1548 Size: 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2122 4183 136 Size: 107 122 71 631 605 450 2061 1525 Fragments arranged by size: 2061 1525 631 605 450 122 107 71 BglII A'GATC_T Cuts at: 5567 5567 Size: 5572 BlpI GC'TnA_GC Cuts at: 2989 2989 Size: 5572 Bme1580I G_kGCm'C Cuts at: 1790 2191 3633 4789 1790 Size: 401 1442 1156 2573 Fragments arranged by size: 2573 1442 1156 401 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5572 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3303 4261 442 Size: 416 53 83 399 24 1886 958 1753 Fragments arranged by size: 1886 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2355 4232 1239 Size: 974 142 1877 2579 Fragments arranged by size: 2579 1877 974 142 Bpu10I CC'TnA_GC Cuts at: 2040 2490 2040 Size: 450 5122 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3697 4562 4860 3697 Size: 865 298 4409 Fragments arranged by size: 4409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4235 882 Size: 617 2736 2219 Fragments arranged by size: 2736 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2705 3363 493 Size: 711 1501 658 2702 Fragments arranged by size: 2702 1501 711 658 BsaBI GATnn'nnATC Cuts at: 5566 5566 Size: 5572 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3379 3761 4396 275 Size: 53 83 186 936 1846 382 635 1451 Fragments arranged by size: 1846 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4005 4403 4677 4824 5235 4005 Size: 398 274 147 411 4342 Fragments arranged by size: 4342 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2406 2436 1178 Size: 30 165 30 1003 30 4314 Fragments arranged by size: 4314 1003 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2319 1186 Size: 1024 109 4439 Fragments arranged by size: 4439 1024 109 BseYI C'CCAG_C Cuts at: 2479 2575 4775 5271 2479 Size: 96 2200 496 2780 Fragments arranged by size: 2780 2200 496 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2232 1163 Size: 573 496 4503 Fragments arranged by size: 4503 573 496 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3022 3783 4811 1540 Size: 275 186 1021 761 1028 2301 Fragments arranged by size: 2301 1028 1021 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3633 3718 4789 3633 Size: 85 1071 4416 Fragments arranged by size: 4416 1071 85 BsmI GAATG_Cn' Cuts at: 3083 3176 3083 Size: 93 5479 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2191 2402 2891 3633 3718 4789 1391 Size: 399 401 211 489 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 489 401 399 211 85 BspEI T'CCGG_A Cuts at: 5235 5235 Size: 5572 BspHI T'CATG_A Cuts at: 3461 5191 3461 Size: 1730 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2654 2927 844 Size: 1810 273 3489 Fragments arranged by size: 3489 1810 273 BsrBI CCG'CTC Cuts at: 1394 1823 2685 3459 1394 Size: 429 862 774 3507 Fragments arranged by size: 3507 862 774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2236 2353 4067 4241 65 Size: 1183 988 117 1714 174 1396 Fragments arranged by size: 1714 1396 1183 988 174 117 BsrFI r'CCGG_y Cuts at: 1700 1810 4216 4403 1700 Size: 110 2406 187 2869 Fragments arranged by size: 2869 2406 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5572 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5572 BssSI C'ACGA_G Cuts at: 3632 4644 3632 Size: 1012 4560 BstAPI GCAn_nnn'nTGC Cuts at: 794 2226 794 Size: 1432 4140 BstBI TT'CG_AA Cuts at: 4376 4376 Size: 5572 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5572 BstYI r'GATC_y Cuts at: 1972 2902 3269 3654 3671 5123 5209 5221 5567 Size: 930 367 385 17 1452 86 12 346 Cuts at: 5567 1972 Size: 1977 Fragments arranged by size: 1977 1452 930 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2643 2643 Size: 5572 BtgI C'CryG_G Cuts at: 513 1065 2433 513 Size: 552 1368 3652 Fragments arranged by size: 3652 1368 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4833 Fragments arranged by size: 4833 683 56 BtsI GCAGTG_nn' Cuts at: 3088 3882 3902 3088 Size: 794 20 4758 Fragments arranged by size: 4758 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2876 2876 Size: 5572 DraIII CAC_nnn'GTG Cuts at: 2026 2026 Size: 5572 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4579 809 Size: 507 3263 1802 Fragments arranged by size: 3263 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2430 2712 Size: 54 1425 50 275 18 243 357 282 Cuts at: 2712 2734 3019 3908 5537 8 Size: 22 285 889 1629 43 Fragments arranged by size: 1629 1425 889 357 285 282 275 243 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 3019 1537 Size: 275 1207 4090 Fragments arranged by size: 4090 1207 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2151 2769 3502 1353 Size: 798 618 733 3423 Fragments arranged by size: 3423 798 733 618 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2552 4159 4533 4679 387 Size: 1064 138 963 1607 374 146 1280 Fragments arranged by size: 1607 1280 1064 963 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2355 2455 3635 4232 5019 5287 Size: 393 581 142 100 1180 597 787 268 Cuts at: 5287 1239 Size: 1524 Fragments arranged by size: 1524 1180 787 597 581 393 268 142 100 EcoNI CCTnn'n_nnAGG Cuts at: 2145 2145 Size: 5572 EcoO109I rG'GnC_Cy Cuts at: 2666 2666 Size: 5572 EcoRI G'AATT_C Cuts at: 2697 2783 2881 3259 2697 Size: 86 98 378 5010 Fragments arranged by size: 5010 378 98 86 FspI TGC'GCA Cuts at: 4078 4078 Size: 5572 HaeII r_GCGC'y Cuts at: 1678 4719 1678 Size: 3041 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2842 2874 4213 4245 4287 Size: 32 135 32 1270 32 1339 32 42 Cuts at: 4287 4319 1373 Size: 32 2626 Fragments arranged by size: 2626 1339 1270 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2582 2916 3162 669 Size: 988 281 644 334 246 3079 Fragments arranged by size: 3079 988 644 334 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5572 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KpnI G_GTAC'C Cuts at: 2897 2897 Size: 5572 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2743 4686 4870 1305 Size: 1438 1943 184 2007 Fragments arranged by size: 2007 1943 1438 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2075 2432 2714 2736 10 Size: 54 1425 343 243 357 282 22 2846 Fragments arranged by size: 2846 1425 357 343 282 243 54 22 MslI CAynn'nnrTG Cuts at: 518 1166 3530 3889 4048 518 Size: 648 2364 359 159 2042 Fragments arranged by size: 2364 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2483 2836 2988 3665 4813 5058 1606 Size: 877 353 152 677 1148 245 2120 Fragments arranged by size: 2120 1148 877 677 353 245 152 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5020 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5572 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2930 4475 1132 Size: 97 1701 1545 2229 Fragments arranged by size: 2229 1701 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5572 PciI A'CATG_T Cuts at: 4471 4471 Size: 5572 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5572 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PmlI CAC'GTG Cuts at: 2705 2705 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2666 2666 Size: 5572 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 3142 3142 Size: 5572 PstI C_TGCA'G Cuts at: 830 2365 2924 830 Size: 1535 559 3478 Fragments arranged by size: 3478 1535 559 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 PvuII CAG'CTG Cuts at: 2483 2483 Size: 5572 SalI G'TCGA_C Cuts at: 1936 2914 1936 Size: 978 4594 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 3820 5532 3820 Size: 1712 3860 SexAI A'CCwGG_T Cuts at: 2078 2078 Size: 5572 SfcI C'TryA_G Cuts at: 826 1045 2085 2361 2920 4055 4736 4927 826 Size: 219 1040 276 559 1135 681 191 1471 Fragments arranged by size: 1471 1135 1040 681 559 276 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5572 SmaI CCC'GGG Cuts at: 1872 2899 1872 Size: 1027 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3676 4577 4839 820 Size: 197 926 1733 901 262 1553 Fragments arranged by size: 1733 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2930 1132 Size: 1798 3774 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5572 StyI C'CwwG_G Cuts at: 513 1065 3011 513 Size: 552 1946 3074 Fragments arranged by size: 3074 1946 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3599 3616 3769 3954 4399 325 Size: 1255 2019 17 153 185 445 1498 Fragments arranged by size: 2019 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 2647 3818 Size: 274 80 33 51 1443 237 433 1171 Cuts at: 3818 5530 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1171 433 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2406 2438 5532 5564 2406 Size: 32 3094 32 2414 Fragments arranged by size: 3094 2414 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5572 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5572 XmaI C'CCGG_G Cuts at: 1870 2897 1870 Size: 1027 4545 XmnI GAAnn'nnTTC Cuts at: 3701 3701 Size: 5572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3380 276 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI