################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 196 GTAGAC GT^[AC][GT]AC AlwNI 256 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 304 AGCGCT AGC^GCT ApaI 12 GGGCCC GGGCC^C AvaII 445 GGACC G^G[AT]CC BanII 12 GGGCCC G[GA]GC[CT]^C BbrPI 229 CACGTG CAC^GTG BglI 366 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 12 GGGCCC G[AGT]GC[ACT]^C BsaAI 229 CACGTG [CT]AC^GT[GA] BsaI 88 GGTCTC GGTCTC(1/5) BsaWI 442 ACCGGA [AT]^CCGG[AT] BseRI 273 GAGGAG GAGGAG(10/8) BsgI 28 GTGCAG GTGCAG(16/14) BsmAI 89 GTCTC GTCTC(1/5) BsmFI 252 GTCCC GGGAC(10/14) Bsp1286I 12 GGGCCC G[AGT]GC[ACT]^C BspMI 30 GCAGGT ACCTGC(4/8) BsrDI 83 GCAATG GCAATG(2/0) BstAPI 21 GCAGTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DpnII 416 GATC ^GATC DraII 11 AGGGCCC [GA]G^G[ACGT]CC[CT] DrdI 199 GACATCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 304 AGCGCT AGC^GCT EcoO109I 11 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoT14I 8 CCAAGG C^C[AT][AT]GG FauI 15 CCCGC CCCGC(4/6) HaeII 304 AGCGCT [GA]GCGC^[CT] HapII 443 CCGG C^CGG HgaI 41 GACGC GACGC(5/10) HinfI 250 GAGTC G^A[ACGT]TC HpaII 443 CCGG C^CGG MaeII 230 ACGT A^CGT MaeIII 227 GTCAC ^GT[ACGT]AC MboI 416 GATC ^GATC MseI 320 TTAA T^TAA MslI 93 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 443 CCGG C^CGG NdeII 416 GATC ^GATC NlaIII 269 CATG CATG^ PflMI 255 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 250 GAGTC GAGTC(4/5) PmaCI 229 CACGTG CAC^GTG PmlI 229 CACGTG CAC^GTG PstI 19 CTGCAG CTGCA^G PvuII 59 CAGCTG CAG^CTG Sau3AI 416 GATC ^GATC StuI 294 AGGCCT AGG^CCT StyI 8 CCAAGG C^C[AT][AT]GG Tru9I 320 TTAA T^TAA Tsp45I 227 GTCAC ^GT[GC]AC Tsp509I 322 AATT ^AATT TspRI 70 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 255 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 361 CCAAAGCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 79 CGCG CG^CG 131 CGCG AfaI 144 GTAC GT^AC 313 GTAC BbsI 48 GAAGAC GAAGAC(2/6) 34 GTCTTC BbvI 18 GCTGC GCAGC(8/12) 150 GCTGC BpmI 115 CTGGAG CTGGAG(16/14) 154 CTGGAG BpuAI 48 GAAGAC GAAGAC(2/6) 34 GTCTTC BstUI 79 CGCG CG^CG 131 CGCG BstXI 237 CCAGATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 361 CCAAAGCCCTGG Cac8I 14 GCCCGC GC[ACGT]^[ACGT]GC 134 GCACGC HphI 99 GGTGA GGTGA(8/7) 247 GGTGA MboII 48 GAAGA GAAGA(8/7) 35 TCTTC MspA1I 16 CCGCTG C[AC]G^C[GT]G 59 CAGCTG MvnI 79 CGCG CG^CG 131 CGCG RsaI 144 GTAC GT^AC 313 GTAC SfcI 19 CTGCAG C^T[GA][CT]AG 139 CTACAG TseI 18 GCTGC G^C[AT]GC 150 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI CfoI Cfr13I EcoRII Fnu4HI FokI HaeIII HhaI HinP1I ItaI MnlI MvaI MwoI NlaIV Sau96I ScrFI