(Linear) (Six-base) MAPSORT of: KIEE5180.seq Check: 8122 from: 1 to: 456 KIEE5180.orf With 182 enzymes: SgfI * January 29, 2014 11:49 .. AccI GT'mk_AC Cuts at: 0 197 456 Size: 197 259 AfeI AGC'GCT Cuts at: 0 306 456 Size: 306 150 AlwNI CAG_nnn'CTG Cuts at: 0 261 456 Size: 261 195 ApaI G_GGCC'C Cuts at: 0 16 456 Size: 16 440 BanII G_rGCy'C Cuts at: 0 16 456 Size: 16 440 BbsI GAAGACnn'nnnn_ Cuts at: 0 27 55 456 Size: 27 28 401 Fragments arranged by size: 401 28 27 BglI GCCn_nnn'nGGC Cuts at: 0 372 456 Size: 372 84 Bme1580I G_kGCm'C Cuts at: 0 16 456 Size: 16 440 BmrI ACTGGGnnnn_n' Cuts at: 0 271 456 Size: 271 185 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 175 456 Size: 136 39 281 Fragments arranged by size: 281 136 39 BsaI GGTCTCn'nnnn_ Cuts at: 0 94 456 Size: 94 362 BsaAI yAC'GTr Cuts at: 0 231 456 Size: 231 225 BsaWI w'CCGG_w Cuts at: 0 442 456 Size: 442 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 288 456 Size: 288 168 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 456 Size: 49 407 Bsp1286I G_dGCh'C Cuts at: 0 16 456 Size: 16 440 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 21 456 Size: 21 435 BsrDI GCAATG_nn' Cuts at: 0 90 456 Size: 90 366 BstAPI GCAn_nnn'nTGC Cuts at: 0 27 456 Size: 27 429 BstXI CCAn_nnnn'nTGG Cuts at: 0 244 368 456 Size: 244 124 88 Fragments arranged by size: 244 124 88 BtsI GCAGTG_nn' Cuts at: 0 69 456 Size: 69 387 DrdI GACnn_nn'nnGTC Cuts at: 0 205 456 Size: 205 251 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 175 456 Size: 136 39 281 Fragments arranged by size: 281 136 39 EcoO109I rG'GnC_Cy Cuts at: 0 12 456 Size: 12 444 HaeII r_GCGC'y Cuts at: 0 308 456 Size: 308 148 MslI CAynn'nnrTG Cuts at: 0 97 456 Size: 97 359 MspA1I CmG'CkG Cuts at: 0 18 61 456 Size: 18 43 395 Fragments arranged by size: 395 43 18 PflMI CCAn_nnn'nTGG Cuts at: 0 261 456 Size: 261 195 PmlI CAC'GTG Cuts at: 0 231 456 Size: 231 225 PspOMI G'GGCC_C Cuts at: 0 12 456 Size: 12 444 PstI C_TGCA'G Cuts at: 0 23 456 Size: 23 433 PvuII CAG'CTG Cuts at: 0 61 456 Size: 61 395 SfcI C'TryA_G Cuts at: 0 19 139 456 Size: 19 120 317 Fragments arranged by size: 317 120 19 StuI AGG'CCT Cuts at: 0 296 456 Size: 296 160 StyI C'CwwG_G Cuts at: 0 8 456 Size: 8 448 TatI w'GTAC_w Cuts at: 0 143 456 Size: 143 313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 368 456 Size: 368 88 Enzymes that do cut: AccI AfeI AlwNI ApaI BanII BbsI BglI Bme1580I BmrI BpmI BsaI BsaAI BsaWI BseRI BsgI Bsp1286I BspMI BsrDI BstAPI BstXI BtsI DrdI Eco57MI EcoO109I HaeII MslI MspA1I PflMI PmlI PspOMI PstI PvuII SfcI StuI StyI TatI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI