Restriction map for
pFN21AE5152
(Circular) (Six-base) MAPSORT of: pFN21AE5152.seq Check: 2784 from: 1 to: 4966
pFN21AE5152 4966 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2776 278
Size: 53 83 186 936 1240 2468
Fragments arranged by size:
2468 1240 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2309 1937
Size: 372 4594
Acc65I G'GTAC_C
Cuts at: 2287 2287
Size: 4966
AclI AA'CG_TT
Cuts at: 1563 3093 3466 1563
Size: 1530 373 3063
Fragments arranged by size:
3063 1530 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3029 4413 4681 1632
Size: 1397 1384 268 1917
Fragments arranged by size:
1917 1397 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4769
AflIII A'CryG_T
Cuts at: 1203 3865 4842 1203
Size: 2662 977 1327
Fragments arranged by size:
2662 1327 977
AgeI A'CCGG_T
Cuts at: 3797 3797
Size: 4966
AhdI GACnn_n'nnGTC
Cuts at: 3695 3695
Size: 4966
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4966
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4966
AlwNI CAG_nnn'CTG
Cuts at: 2709 4281 2709
Size: 1572 3394
ApaLI G'TGCA_C
Cuts at: 3023 4179 3023
Size: 1156 3810
ApoI r'AATT_y
Cuts at: 1493 2275 2501 2653 4762 1493
Size: 782 226 152 2109 1697
Fragments arranged by size:
2109 1697 782 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3520 160
Size: 872 2488 1606
Fragments arranged by size:
2488 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4966
AvaI C'yCGr_G
Cuts at: 1870 1943 2291 1870
Size: 73 348 4545
Fragments arranged by size:
4545 348 73
BamHI G'GATC_C
Cuts at: 2296 2663 2296
Size: 367 4599
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2287 3742 618
Size: 325 217 627 500 1455 1842
Fragments arranged by size:
1842 1455 627 500 325 217
BanII G_rGCy'C
Cuts at: 1391 2130 2285 1391
Size: 739 155 4072
Fragments arranged by size:
4072 739 155
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3995
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2149 2183 3157 3191 1903
Size: 34 50 34 128 34 974 34 3678
Fragments arranged by size:
3678 974 128 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2858 4068 2858
Size: 1210 3756
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4966
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3577 136
Size: 107 122 71 631 605 1905 1525
Fragments arranged by size:
1905 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4961 4961
Size: 4966
BlpI GC'TnA_GC
Cuts at: 2254 2383 2254
Size: 129 4837
Bme1580I G_kGCm'C
Cuts at: 1790 3027 4183 1790
Size: 1237 1156 2573
Fragments arranged by size:
2573 1237 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4966
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2697 3655 442
Size: 416 53 83 399 24 1280 958 1753
Fragments arranged by size:
1753 1280 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 3626 1239
Size: 860 1527 2579
Fragments arranged by size:
2579 1527 860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3091 3956 4254 3091
Size: 865 298 3803
Fragments arranged by size:
3803 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3629 882
Size: 617 2130 2219
Fragments arranged by size:
2219 2130 617
BsaAI yAC'GTr
Cuts at: 493 1204 2757 493
Size: 711 1553 2702
Fragments arranged by size:
2702 1553 711
BsaBI GATnn'nnATC
Cuts at: 4960 4960
Size: 4966
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2773 3155 3790 275
Size: 53 83 186 936 1240 382 635 1451
Fragments arranged by size:
1451 1240 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2210 3399 3797 4071 4218 4629 2210
Size: 1189 398 274 147 411 2547
Fragments arranged by size:
2547 1189 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4741
Fragments arranged by size:
4741 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4966
BseYI C'CCAG_C
Cuts at: 2138 4169 4665 2138
Size: 2031 496 2439
Fragments arranged by size:
2439 2031 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4393
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2416 3177 4205 1540
Size: 275 186 415 761 1028 2301
Fragments arranged by size:
2301 1028 761 415 275 186
BsiHKAI G_wGCw'C
Cuts at: 3027 3112 4183 3027
Size: 85 1071 3810
Fragments arranged by size:
3810 1071 85
BsmI GAATG_Cn'
Cuts at: 2477 2570 2477
Size: 93 4873
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2285 3027 3112 4183 1391
Size: 399 340 155 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 340 155 85
BspEI T'CCGG_A
Cuts at: 4629 4629
Size: 4966
BspHI T'CATG_A
Cuts at: 2172 2855 4585 2172
Size: 683 1730 2553
Fragments arranged by size:
2553 1730 683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2321 844
Size: 1477 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2853 1394
Size: 429 1030 3507
Fragments arranged by size:
3507 1030 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3461 3635 65
Size: 1183 2213 174 1396
Fragments arranged by size:
2213 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3610 3797 1700
Size: 110 1800 187 2869
Fragments arranged by size:
2869 1800 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4966
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4966
BssSI C'ACGA_G
Cuts at: 3026 4038 3026
Size: 1012 3954
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4966
BstBI TT'CG_AA
Cuts at: 3770 3770
Size: 4966
BstEII G'GTnAC_C
Cuts at: 1184 2014 2213 1184
Size: 830 199 3937
Fragments arranged by size:
3937 830 199
BstYI r'GATC_y
Cuts at: 1972 2296 2663 3048 3065 4517 4603 4615 4961
Size: 324 367 385 17 1452 86 12 346
Cuts at: 4961 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 324 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2154 513
Size: 552 1089 3325
Fragments arranged by size:
3325 1089 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4227
Fragments arranged by size:
4227 683 56
BtsI GCAGTG_nn'
Cuts at: 2482 3276 3296 2482
Size: 794 20 4152
Fragments arranged by size:
4152 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4966
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4931
DraI TTT'AAA
Cuts at: 2270 2270
Size: 4966
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3973 809
Size: 507 2657 1802
Fragments arranged by size:
2657 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2200 2413 3302
Size: 54 1425 50 275 18 370 213 889
Cuts at: 3302 4931 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 370 275 213 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2413 1537
Size: 275 601 4090
Fragments arranged by size:
4090 601 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2896 1353
Size: 1543 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3553 3927 4073 387
Size: 1064 138 1964 374 146 1280
Fragments arranged by size:
1964 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 3029 3626 4413 4681 1239
Size: 393 467 930 597 787 268 1524
Fragments arranged by size:
1524 930 787 597 467 393 268
EcoRI G'AATT_C
Cuts at: 2275 2653 2275
Size: 378 4588
FspI TGC'GCA
Cuts at: 3472 3472
Size: 4966
HaeII r_GCGC'y
Cuts at: 1678 4113 1678
Size: 2435 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2221 2253 3607 3639 3681
Size: 32 135 32 649 32 1354 32 42
Cuts at: 3681 3713 1373
Size: 32 2626
Fragments arranged by size:
2626 1354 649 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2310 2556 669
Size: 988 281 372 246 3079
Fragments arranged by size:
3079 988 372 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4966
HpaI GTT'AAC
Cuts at: 2556 2556
Size: 4966
KpnI G_GTAC'C
Cuts at: 2291 2291
Size: 4966
MfeI C'AATT_G
Cuts at: 2565 2565
Size: 4966
MluI A'CGCG_T
Cuts at: 4842 4842
Size: 4966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4080 4264 1305
Size: 2775 184 2007
Fragments arranged by size:
2775 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2202 10
Size: 54 1425 343 370 2774
Fragments arranged by size:
2774 1425 370 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2924 3283 3442 518
Size: 648 1005 753 359 159 2042
Fragments arranged by size:
2042 1005 753 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2382 3059 4207 4452 1606
Size: 776 677 1148 245 2120
Fragments arranged by size:
2120 1148 776 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2154 513
Size: 552 1089 3325
Fragments arranged by size:
3325 1089 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4966
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4966
NotI GC'GGCC_GC
Cuts at: 2413 2413
Size: 4966
NspI r_CATG'y
Cuts at: 1132 1229 2324 3869 1132
Size: 97 1095 1545 2229
Fragments arranged by size:
2229 1545 1095 97
PasI CC'CwG_GG
Cuts at: 1348 2020 1348
Size: 672 4294
PciI A'CATG_T
Cuts at: 3865 3865
Size: 4966
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4966
PfoI T'CCnGG_A
Cuts at: 2055 2055
Size: 4966
PmeI GTTT'AAAC
Cuts at: 2270 2270
Size: 4966
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 4548 4580 3035
Size: 32 1481 32 3421
Fragments arranged by size:
3421 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4966
PsiI TTA'TAA
Cuts at: 2536 2536
Size: 4966
PstI C_TGCA'G
Cuts at: 830 2318 830
Size: 1488 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4966
SalI G'TCGA_C
Cuts at: 1936 2308 1936
Size: 372 4594
SbfI CC_TGCA'GG
Cuts at: 2318 2318
Size: 4966
ScaI AGT'ACT
Cuts at: 3214 4926 3214
Size: 1712 3254
SfcI C'TryA_G
Cuts at: 826 1045 2314 3449 4130 4321 826
Size: 219 1269 1135 681 191 1471
Fragments arranged by size:
1471 1269 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4966
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4966
SmaI CCC'GGG
Cuts at: 1872 2293 1872
Size: 421 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3070 3971 4233 820
Size: 197 926 1127 901 262 1553
Fragments arranged by size:
1553 1127 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2757 493
Size: 2264 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4966
SphI G_CATG'C
Cuts at: 1132 2324 1132
Size: 1192 3774
SspI AAT'ATT
Cuts at: 5 52 2890 5
Size: 47 2838 2081
Fragments arranged by size:
2838 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4966
StyI C'CwwG_G
Cuts at: 513 1065 2154 2405 513
Size: 552 1089 251 3074
Fragments arranged by size:
3074 1089 552 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2993 3010 3163 3348 3793 325
Size: 1255 1413 17 153 185 445 1498
Fragments arranged by size:
1498 1413 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3212 4924 96
Size: 274 80 33 51 1443 1235 1712 138
Fragments arranged by size:
1712 1443 1235 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4926 4958 4926
Size: 32 4934
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4966
XbaI T'CTAG_A
Cuts at: 2302 2302
Size: 4966
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4966
XmaI C'CCGG_G
Cuts at: 1870 2291 1870
Size: 421 4545
XmnI GAAnn'nnTTC
Cuts at: 3095 3095
Size: 4966
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2774 276
Size: 53 83 186 936 1240 2468
Fragments arranged by size:
2468 1240 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI