################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII AclI AcsI AcyI AflII AhdI ApaI ApoI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BcgI BciVI BfrI BglI BglII BlnI BsaBI BsaHI BsiEI BsiWI BsmBI BspDI BsrBI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoRI EcoT22I FseI FspI HgaI Hin1I HpaI KasI KspI MamI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII SacI SacII ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 2368 GGTACC G^GTACC AccI 1579 GTCGAC GT^[AC][GT]AC AccIII 1526 TCCGGA T^CCGGA AgeI 1532 ACCGGT A^CCGGT AocI 825 CCTGAGG CC^T[ACGT]AGG Aor51HI 2219 AGCGCT AGC^GCT Asp700I 198 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2368 GGTACC G^GTACC BalI 573 TGGCCA TGG^CCA BclI 1718 TGATCA T^GATCA BcnI 93 CCCGG CC^[GC]GG BfaI 1301 CTAG C^TAG BsaI 821 GAGACC GGTCTC(1/5) BseAI 1526 TCCGGA T^CCGGA Bsp1407I 917 TGTACA T^GTACA BspEI 1526 TCCGGA T^CCGGA BspLU11I 1355 ACATGT A^CATGT BsrGI 917 TGTACA T^GTACA BssSI 976 CACGAG CACGAG(-5/-1) Bsu36I 825 CCTGAGG CC^T[ACGT]AGG Eco47III 2219 AGCGCT AGC^GCT Eco81I 825 CCTGAGG CC^T[ACGT]AGG EcoRV 1270 GATATC GAT^ATC FbaI 1718 TGATCA T^GATCA HindIII 135 AAGCTT A^AGCTT KpnI 2368 GGTACC GGTAC^C MaeI 1301 CTAG C^TAG MluNI 573 TGGCCA TGG^CCA MroI 1526 TCCGGA T^CCGGA MscI 573 TGGCCA TGG^CCA MseI 2329 TTAA T^TAA NciI 93 CCCGG CC^[GC]GG PinAI 1532 ACCGGT A^CCGGT PshAI 993 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 1579 GTCGAC G^TCGAC SapI 1281 GCTCTTC GCTCTTC(1/4) SbfI 1002 CCTGCAGG CCTGCA^GG SexAI 651 ACCAGGT A^CC[AT]GGT SmlI 1894 CTCAAG C^T[CT][GA]AG Sse8387I 1002 CCTGCAGG CCTGCA^GG SspBI 917 TGTACA T^GTACA StuI 1338 AGGCCT AGG^CCT TaqI 1580 TCGA T^CGA Tru9I 2329 TTAA T^TAA Tsp509I 952 AATT ^AATT TthHB8I 1580 TCGA T^CGA XmnI 198 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 706 CGCG CG^CG 2256 CGCG AflIII 413 ACACGT A^C[GA][CT]GT 1355 ACATGT Alw44I 417 GTGCAC G^TGCAC 1690 GTGCAC ApaLI 417 GTGCAC G^TGCAC 1690 GTGCAC BbrPI 414 CACGTG CAC^GTG 2134 CACGTG BsaWI 1526 TCCGGA [AT]^CCGG[AT] 1532 ACCGGT BsmI 256 GAATGC GAATGC(1/-1) 1097 GCATTC BspHI 1448 TCATGA T^CATGA 2381 TCATGA BsrDI 1239 CATTGC GCAATG(2/0) 569 GCAATG BstEII 655 GGTGACC G^GT[ACGT]ACC 930 GGTGACC BstPI 655 GGTGACC G^GT[ACGT]ACC 930 GGTGACC BstUI 706 CGCG CG^CG 2256 CGCG BstYI 1412 AGATCC [GA]^GATC[CT] 2162 AGATCC DraII 620 AGGACCT [GA]G^G[ACGT]CC[CT] 1670 GGGTCCT DraIII 650 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 1864 CACAAGGTG EarI 1282 CTCTTC CTCTTC(1/4) 152 GAAGAG EcoO109I 620 AGGACCT [GA]G^G[ACGT]CC[CT] 1670 GGGTCCT EcoO65I 655 GGTGACC G^GT[ACGT]ACC 930 GGTGACC HincII 1579 GTCGAC GT[CT]^[GA]AC 2020 GTTGAC HindII 1579 GTCGAC GT[CT]^[GA]AC 2020 GTTGAC Ksp632I 1282 CTCTTC CTCTTC(1/4) 152 GAAGAG MflI 1412 AGATCC [GA]^GATC[CT] 2162 AGATCC MvnI 706 CGCG CG^CG 2256 CGCG PmaCI 414 CACGTG CAC^GTG 2134 CACGTG PmlI 414 CACGTG CAC^GTG 2134 CACGTG PpuMI 620 AGGACCT [GA]G^G[AT]CC[CT] 1670 GGGTCCT RcaI 1448 TCATGA T^CATGA 2381 TCATGA SphI 274 GCATGC GCATG^C 2315 GCATGC XhoII 1412 AGATCC [GA]^GATC[CT] 2162 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AspI AvaI AvaII BanI BanII BbsI BbvCI BbvI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NaeI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I TspRI Tth111I Van91I XcmI