Restriction map for
pFN21AE2497
(Circular) (Six-base) MAPSORT of: pFN21AE2497.seq Check: 2919 from: 1 to: 7231
pFN21AE2497 7231 bp
With 182 enzymes: SgfI *
January 16, 2014 19:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2972 3001 3248 2972
Size: 29 247 6955
Fragments arranged by size:
6955 247 29
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5041 278
Size: 53 83 186 936 3505 2468
Fragments arranged by size:
3505 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3585 4574 1937
Size: 1648 989 4594
Fragments arranged by size:
4594 1648 989
Acc65I G'GTAC_C
Cuts at: 4373 4552 4373
Size: 179 7052
AclI AA'CG_TT
Cuts at: 1563 5358 5731 1563
Size: 3795 373 3063
Fragments arranged by size:
3795 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2636 4346 5294 6678 6946 1632
Size: 1004 1710 948 1384 268 1917
Fragments arranged by size:
1917 1710 1384 1004 948 268
AfeI AGC'GCT
Cuts at: 4226 4226
Size: 7231
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7034
AflIII A'CryG_T
Cuts at: 1203 2418 3360 6130 7107 1203
Size: 1215 942 2770 977 1327
Fragments arranged by size:
2770 1327 1215 977 942
AgeI A'CCGG_T
Cuts at: 3537 6062 3537
Size: 2525 4706
AhdI GACnn_n'nnGTC
Cuts at: 5960 5960
Size: 7231
AleI CACnn'nnGTG
Cuts at: 1166 2933 4511 1166
Size: 1767 1578 3886
Fragments arranged by size:
3886 1767 1578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3125 3256 1184
Size: 1941 131 5159
Fragments arranged by size:
5159 1941 131
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 4274 4306 2534
Size: 32 1708 32 5459
Fragments arranged by size:
5459 1708 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2272 2629 4043 4131 4177 4974 6546 2272
Size: 357 1414 88 46 797 1572 2957
Fragments arranged by size:
2957 1572 1414 797 357 88 46
ApaLI G'TGCA_C
Cuts at: 2422 3695 5288 6444 2422
Size: 1273 1593 1156 3209
Fragments arranged by size:
3209 1593 1273 1156
ApoI r'AATT_y
Cuts at: 1493 4540 4766 4918 7027 1493
Size: 3047 226 152 2109 1697
Fragments arranged by size:
3047 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5785 160
Size: 872 4753 1606
Fragments arranged by size:
4753 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7231
AvaI C'yCGr_G
Cuts at: 1870 1943 2756 4198 4431 4556 1870
Size: 73 813 1442 233 125 4545
Fragments arranged by size:
4545 1442 813 233 125 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3370 3403 3370
Size: 33 7198
BamHI G'GATC_C
Cuts at: 4561 4928 4561
Size: 367 6864
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3426 4073 4373 4552 6007
Size: 325 217 627 1639 647 300 179 1455
Cuts at: 6007 618
Size: 1842
Fragments arranged by size:
1842 1639 1455 647 627 325 300 217
179
BanII G_rGCy'C
Cuts at: 1391 2136 2259 2865 4550 1391
Size: 745 123 606 1685 4072
Fragments arranged by size:
4072 1685 745 606 123
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2252 2643 2895 3228 3303 3364 928
Size: 971 353 391 252 333 75 61 4795
Fragments arranged by size:
4795 971 391 353 333 252 75 61
BbvCI CC'TCA_GC
Cuts at: 2585 3096 3347 2585
Size: 511 251 6469
Fragments arranged by size:
6469 511 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5422 5456 1903
Size: 34 3485 34 3678
Fragments arranged by size:
3678 3485 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5123 6333 5123
Size: 1210 6021
BclI T'GATC_A
Cuts at: 1548 3723 1548
Size: 2175 5056
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5842 136
Size: 107 122 71 631 605 4170 1525
Fragments arranged by size:
4170 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7226 7226
Size: 7231
BlpI GC'TnA_GC
Cuts at: 2237 2604 2744 2795 3639 4359 4648 2237
Size: 367 140 51 844 720 289 4820
Fragments arranged by size:
4820 844 720 367 289 140 51
Bme1580I G_kGCm'C
Cuts at: 1790 2426 3699 3936 5292 6448 1790
Size: 636 1273 237 1356 1156 2573
Fragments arranged by size:
2573 1356 1273 1156 636 237
BmgBI CAC'GTC
Cuts at: 1318 3004 3694 1318
Size: 1686 690 4855
Fragments arranged by size:
4855 1686 690
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4962 5920 442
Size: 416 53 83 399 24 3545 958 1753
Fragments arranged by size:
3545 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2453 3087 3701 4316 5891 1239
Size: 1055 159 634 614 615 1575 2579
Fragments arranged by size:
2579 1575 1055 634 615 614 159
Bpu10I CC'TnA_GC
Cuts at: 2585 3096 3347 3680 2585
Size: 511 251 333 6136
Fragments arranged by size:
6136 511 333 251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3884 5356 6221 6519 3884
Size: 1472 865 298 4596
Fragments arranged by size:
4596 1472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2820 5894 882
Size: 617 1321 3074 2219
Fragments arranged by size:
3074 2219 1321 617
BsaAI yAC'GTr
Cuts at: 493 1204 2296 2421 4141 5022 493
Size: 711 1092 125 1720 881 2702
Fragments arranged by size:
2702 1720 1092 881 711 125
BsaBI GATnn'nnATC
Cuts at: 7225 7225
Size: 7231
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5038 5420 6055 275
Size: 53 83 186 936 3505 382 635 1451
Fragments arranged by size:
3505 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3531 3537 5664 6062 6336 6483 6894 3531
Size: 6 2127 398 274 147 411 3868
Fragments arranged by size:
3868 2127 411 398 274 147 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2504 2534 3699 3729 4401
Size: 30 165 30 1101 30 1165 30 672
Cuts at: 4401 4431 1178
Size: 30 3978
Fragments arranged by size:
3978 1165 1101 672 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2657 3271 4409 1186
Size: 958 513 614 1138 4008
Fragments arranged by size:
4008 1138 958 614 513
BseYI C'CCAG_C
Cuts at: 2065 2288 3498 3555 3684 6434 6930 2065
Size: 223 1210 57 129 2750 496 2366
Fragments arranged by size:
2750 2366 1210 496 223 129 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3899 4121 4163 4253 1163
Size: 573 2163 222 42 90 4141
Fragments arranged by size:
4141 2163 573 222 90 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4681 5442 6470 1540
Size: 275 186 2680 761 1028 2301
Fragments arranged by size:
2680 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 2426 2801 3699 5292 5377 6448 2238
Size: 188 375 898 1593 85 1071 3021
Fragments arranged by size:
3021 1593 1071 898 375 188 85
BsmI GAATG_Cn'
Cuts at: 2267 3102 4742 4835 2267
Size: 835 1640 93 4663
Fragments arranged by size:
4663 1640 835 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2238 2259 2426 2801 2865 3699
Size: 399 346 102 21 167 375 64 834
Cuts at: 3699 3936 4550 5292 5377 6448 1391
Size: 237 614 742 85 1071 2174
Fragments arranged by size:
2174 1071 834 742 614 399 375 346
237 167 102 85 64 21
BspEI T'CCGG_A
Cuts at: 3531 6894 3531
Size: 3363 3868
BspHI T'CATG_A
Cuts at: 3453 4386 5120 6850 3453
Size: 933 734 1730 3834
Fragments arranged by size:
3834 1730 933 734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2819 2972 3001 3248 4194 4293 4586 844
Size: 1975 153 29 247 946 99 293 3489
Fragments arranged by size:
3489 1975 946 293 247 153 99 29
BsrBI CCG'CTC
Cuts at: 1394 1823 5118 1394
Size: 429 3295 3507
Fragments arranged by size:
3507 3295 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2581 3243 5726 5900 65
Size: 1183 1333 662 2483 174 1396
Fragments arranged by size:
2483 1396 1333 1183 662 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2226 2432 3537 3634 3740 5875
Size: 110 246 170 206 1105 97 106 2135
Cuts at: 5875 6062 1700
Size: 187 2869
Fragments arranged by size:
2869 2135 1105 246 206 187 170 110
106 97
BsrGI T'GTAC_A
Cuts at: 96 2922 96
Size: 2826 4405
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7231
BssSI C'ACGA_G
Cuts at: 2981 5291 6303 2981
Size: 2310 1012 3909
Fragments arranged by size:
3909 2310 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7231
BstBI TT'CG_AA
Cuts at: 6035 6035
Size: 7231
BstEII G'GTnAC_C
Cuts at: 1184 2660 2935 1184
Size: 1476 275 5480
Fragments arranged by size:
5480 1476 275
BstXI CCAn_nnnn'nTGG
Cuts at: 2847 2950 3622 2847
Size: 103 672 6456
Fragments arranged by size:
6456 672 103
BstYI r'GATC_y
Cuts at: 1972 3417 4167 4561 4928 5313 5330 6782 6868
Size: 1445 750 394 367 385 17 1452 86
Cuts at: 6868 6880 7226 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1445 750 394 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2831 2831
Size: 7231
BtgI C'CryG_G
Cuts at: 513 1065 2427 2787 2821 3438 3922 3942 4305
Size: 552 1362 360 34 617 484 20 363
Cuts at: 4305 513
Size: 3439
Fragments arranged by size:
3439 1362 617 552 484 363 360 34
20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6492
Fragments arranged by size:
6492 683 56
BtsI GCAGTG_nn'
Cuts at: 2036 3214 4747 5541 5561 2036
Size: 1178 1533 794 20 3706
Fragments arranged by size:
3706 1533 1178 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7196
DraI TTT'AAA
Cuts at: 4535 4535
Size: 7231
DraIII CAC_nnn'GTG
Cuts at: 2660 3874 2660
Size: 1214 6017
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6238 809
Size: 507 4922 1802
Fragments arranged by size:
4922 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2430 2578 2977
Size: 54 1425 50 275 18 600 148 399
Cuts at: 2977 3742 4678 5567 7196 8
Size: 765 936 889 1629 43
Fragments arranged by size:
1629 1425 936 889 765 600 399 275
148 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4678 1537
Size: 275 2866 4090
Fragments arranged by size:
4090 2866 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2152 3293 5161 1353
Size: 799 1141 1868 3423
Fragments arranged by size:
3423 1868 1141 799
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5818 6192 6338 387
Size: 1064 138 4229 374 146 1280
Fragments arranged by size:
4229 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2453 2636 3087 3701 4316 4346
Size: 393 662 159 183 451 614 615 30
Cuts at: 4346 5294 5891 6678 6946 1239
Size: 948 597 787 268 1524
Fragments arranged by size:
1524 948 787 662 615 614 597 451
393 268 183 159 30
EcoO109I rG'GnC_Cy
Cuts at: 2626 3676 2626
Size: 1050 6181
EcoRI G'AATT_C
Cuts at: 4540 4918 4540
Size: 378 6853
EcoRV GAT'ATC
Cuts at: 3277 3277
Size: 7231
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2660 2660
Size: 7231
FspI TGC'GCA
Cuts at: 5737 5737
Size: 7231
HaeII r_GCGC'y
Cuts at: 1678 2533 4102 4228 6378 1678
Size: 855 1569 126 2150 2531
Fragments arranged by size:
2531 2150 1569 855 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2504 2536 3266 3298 3699
Size: 32 135 32 932 32 730 32 401
Cuts at: 3699 3731 4005 4037 4401 4433 5872 5904 5946
Size: 32 274 32 364 32 1439 32 42
Cuts at: 5946 5978 1373
Size: 32 2626
Fragments arranged by size:
2626 1439 932 730 401 364 274 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3586 4027 4575 4821 669
Size: 988 281 1648 441 548 246 3079
Fragments arranged by size:
3079 1648 988 548 441 281 246
HindIII A'AGCT_T
Cuts at: 748 2140 748
Size: 1392 5839
HpaI GTT'AAC
Cuts at: 4821 4821
Size: 7231
KpnI G_GTAC'C
Cuts at: 4377 4556 4377
Size: 179 7052
MfeI C'AATT_G
Cuts at: 4830 4830
Size: 7231
MluI A'CGCG_T
Cuts at: 7107 7107
Size: 7231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3402 6345 6529 1305
Size: 2097 2943 184 2007
Fragments arranged by size:
2943 2097 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2580 10
Size: 54 1425 343 748 4661
Fragments arranged by size:
4661 1425 748 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2933 2948 3107 3548 3620 3921 4511
Size: 648 1767 15 159 441 72 301 590
Cuts at: 4511 5189 5548 5707 518
Size: 678 359 159 2042
Fragments arranged by size:
2042 1767 678 648 590 441 359 301
159 159 72 15
MspA1I CmG'CkG
Cuts at: 1606 2989 4018 4121 4647 5324 6472 6717 1606
Size: 1383 1029 103 526 677 1148 245 2120
Fragments arranged by size:
2120 1383 1148 1029 677 526 245 103
NaeI GCC'GGC
Cuts at: 2058 2434 3636 2058
Size: 376 1202 5653
Fragments arranged by size:
5653 1202 376
NcoI C'CATG_G
Cuts at: 513 1065 2427 2821 3922 3942 513
Size: 552 1362 394 1101 20 3802
Fragments arranged by size:
3802 1362 1101 552 394 20
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7231
NgoMIV G'CCGG_C
Cuts at: 2056 2432 3634 2056
Size: 376 1202 5653
Fragments arranged by size:
5653 1202 376
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7231
NotI GC'GGCC_GC
Cuts at: 4678 4678
Size: 7231
NspI r_CATG'y
Cuts at: 1132 1229 2283 2612 3364 4324 4589 6134 1132
Size: 97 1054 329 752 960 265 1545 2229
Fragments arranged by size:
2229 1545 1054 960 752 329 265 97
PasI CC'CwG_GG
Cuts at: 1348 4365 1348
Size: 3017 4214
PciI A'CATG_T
Cuts at: 3360 6130 3360
Size: 2770 4461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2272 3406 3874 3943 1108
Size: 1164 1134 468 69 4396
Fragments arranged by size:
4396 1164 1134 468 69
PfoI T'CCnGG_A
Cuts at: 2877 4293 2877
Size: 1416 5815
PmeI GTTT'AAAC
Cuts at: 4535 4535
Size: 7231
PmlI CAC'GTG
Cuts at: 2421 4141 2421
Size: 1720 5511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 4427 4459 5300 5332 6813 6845 2975
Size: 32 1420 32 841 32 1481 32 3361
Fragments arranged by size:
3361 1481 1420 841 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2626 3676 2626
Size: 1050 6181
PshAI GACnn'nnGTC
Cuts at: 1613 3002 1613
Size: 1389 5842
PsiI TTA'TAA
Cuts at: 4801 4801
Size: 7231
PstI C_TGCA'G
Cuts at: 830 2216 2484 3012 3523 3865 4120 4583 830
Size: 1386 268 528 511 342 255 463 3478
Fragments arranged by size:
3478 1386 528 511 463 342 268 255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7231
PvuII CAG'CTG
Cuts at: 2989 4018 4121 2989
Size: 1029 103 6099
Fragments arranged by size:
6099 1029 103
SalI G'TCGA_C
Cuts at: 1936 3584 4573 1936
Size: 1648 989 4594
Fragments arranged by size:
4594 1648 989
SapI GCTCTTCn'nnn_
Cuts at: 3293 3293
Size: 7231
SbfI CC_TGCA'GG
Cuts at: 3012 4583 3012
Size: 1571 5660
ScaI AGT'ACT
Cuts at: 5479 7191 5479
Size: 1712 5519
SexAI A'CCwGG_T
Cuts at: 2656 2656
Size: 7231
SfcI C'TryA_G
Cuts at: 826 1045 2212 2480 3008 3519 3608 3861 4116
Size: 219 1167 268 528 511 89 253 255
Cuts at: 4116 4579 5714 6395 6586 826
Size: 463 1135 681 191 1471
Fragments arranged by size:
1471 1167 1135 681 528 511 463 268
255 253 219 191 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7231
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7231
SmaI CCC'GGG
Cuts at: 1872 4558 1872
Size: 2686 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3899 5335 6236 6498 820
Size: 197 926 1956 1436 901 262 1553
Fragments arranged by size:
1956 1553 1436 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5022 493
Size: 4529 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7231
SphI G_CATG'C
Cuts at: 1132 2283 4324 4589 1132
Size: 1151 2041 265 3774
Fragments arranged by size:
3774 2041 1151 265
SspI AAT'ATT
Cuts at: 5 52 5155 5
Size: 47 5103 2081
Fragments arranged by size:
5103 2081 47
StuI AGG'CCT
Cuts at: 1517 3345 1517
Size: 1828 5403
StyI C'CwwG_G
Cuts at: 513 1065 2427 2821 2931 3922 3942 4670 513
Size: 552 1362 394 110 991 20 728 3074
Fragments arranged by size:
3074 1362 991 728 552 394 110 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2096 3251 3968 4507 4511 5258 5275
Size: 1255 516 1155 717 539 4 747 17
Cuts at: 5275 5428 5613 6058 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1155 747 717 539 516 445
185 153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2922 5477 7189
Size: 274 80 33 51 1443 945 2555 1712
Cuts at: 7189 96
Size: 138
Fragments arranged by size:
2555 1712 1443 945 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7191 7223 7191
Size: 32 7199
Tth111I GACn'n_nGTC
Cuts at: 1316 2765 3711 4031 1316
Size: 1449 946 320 4516
Fragments arranged by size:
4516 1449 946 320
XbaI T'CTAG_A
Cuts at: 4567 4567
Size: 7231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2019 2785 2844 2947 3256 3830 4312 4362 2019
Size: 766 59 103 309 574 482 50 4888
Fragments arranged by size:
4888 766 574 482 309 103 59 50
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7231
XmaI C'CCGG_G
Cuts at: 1870 4556 1870
Size: 2686 4545
XmnI GAAnn'nnTTC
Cuts at: 2207 5360 2207
Size: 3153 4078
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5039 276
Size: 53 83 186 936 3505 2468
Fragments arranged by size:
3505 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoICRI EcoNI FseI FspAI KasI NarI NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI