Restriction map for
pFN21AE2497
(Linear) (Six-base) MAPSORT of: KIEE2497.seq Check: 7742 from: 1 to: 2526
KIEE2497.orf
With 182 enzymes: SgfI *
January 16, 2014 17:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 967 996 1243 2526
Size: 967 29 247 1283
Fragments arranged by size:
1283 967 247 29
AccI GT'mk_AC
Cuts at: 0 1580 2526
Size: 1580 946
Acc65I G'GTAC_C
Cuts at: 0 2368 2526
Size: 2368 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 2341 2526
Size: 631 1710 185
Fragments arranged by size:
1710 631 185
AfeI AGC'GCT
Cuts at: 0 2221 2526
Size: 2221 305
AflIII A'CryG_T
Cuts at: 0 413 1355 2526
Size: 413 942 1171
Fragments arranged by size:
1171 942 413
AgeI A'CCGG_T
Cuts at: 0 1532 2526
Size: 1532 994
AleI CACnn'nnGTG
Cuts at: 0 928 2506 2526
Size: 928 1578 20
Fragments arranged by size:
1578 928 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1120 1251 2526
Size: 1120 131 1275
Fragments arranged by size:
1275 1120 131
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 529 561 2269 2301 2526
Size: 529 32 1708 32 225
Fragments arranged by size:
1708 529 225 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 267 624 2038 2126 2172 2526
Size: 267 357 1414 88 46 354
Fragments arranged by size:
1414 357 354 267 88 46
ApaLI G'TGCA_C
Cuts at: 0 417 1690 2526
Size: 417 1273 836
Fragments arranged by size:
1273 836 417
AvaI C'yCGr_G
Cuts at: 0 751 2193 2426 2526
Size: 751 1442 233 100
Fragments arranged by size:
1442 751 233 100
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1365 1398 2526
Size: 1365 33 1128
Fragments arranged by size:
1365 1128 33
BanI G'GyrC_C
Cuts at: 0 1421 2068 2368 2526
Size: 1421 647 300 158
Fragments arranged by size:
1421 647 300 158
BanII G_rGCy'C
Cuts at: 0 131 254 860 2526
Size: 131 123 606 1666
Fragments arranged by size:
1666 606 131 123
BbsI GAAGACnn'nnnn_
Cuts at: 0 247 638 890 1223 1298 1359 2526
Size: 247 391 252 333 75 61 1167
Fragments arranged by size:
1167 391 333 252 247 75 61
BbvCI CC'TCA_GC
Cuts at: 0 580 1091 1342 2526
Size: 580 511 251 1184
Fragments arranged by size:
1184 580 511 251
BclI T'GATC_A
Cuts at: 0 1718 2526
Size: 1718 808
BlpI GC'TnA_GC
Cuts at: 0 232 599 739 790 1634 2354 2526
Size: 232 367 140 51 844 720 172
Fragments arranged by size:
844 720 367 232 172 140 51
Bme1580I G_kGCm'C
Cuts at: 0 421 1694 1931 2526
Size: 421 1273 237 595
Fragments arranged by size:
1273 595 421 237
BmgBI CAC'GTC
Cuts at: 0 999 1689 2526
Size: 999 690 837
Fragments arranged by size:
999 837 690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 448 1082 1696 2311 2526
Size: 289 159 634 614 615 215
Fragments arranged by size:
634 615 614 289 215 159
Bpu10I CC'TnA_GC
Cuts at: 0 580 1091 1342 1675 2526
Size: 580 511 251 333 851
Fragments arranged by size:
851 580 511 333 251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1879 2526
Size: 1879 647
BsaI GGTCTCn'nnnn_
Cuts at: 0 815 2526
Size: 815 1711
BsaAI yAC'GTr
Cuts at: 0 291 416 2136 2526
Size: 291 125 1720 390
Fragments arranged by size:
1720 390 291 125
BsaWI w'CCGG_w
Cuts at: 0 1526 1532 2526
Size: 1526 6 994
Fragments arranged by size:
1526 994 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 499 529 1694 1724 2396 2426 2526
Size: 499 30 1165 30 672 30 100
Fragments arranged by size:
1165 672 499 100 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 652 1266 2404 2526
Size: 139 513 614 1138 122
Fragments arranged by size:
1138 614 513 139 122
BseYI C'CCAG_C
Cuts at: 0 60 283 1493 1550 1679 2526
Size: 60 223 1210 57 129 847
Fragments arranged by size:
1210 847 223 129 60 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1894 2116 2158 2248 2526
Size: 1894 222 42 90 278
Fragments arranged by size:
1894 278 222 90 42
BsiHKAI G_wGCw'C
Cuts at: 0 233 421 796 1694 2526
Size: 233 188 375 898 832
Fragments arranged by size:
898 832 375 233 188
BsmI GAATG_Cn'
Cuts at: 0 262 1097 2526
Size: 262 835 1429
Fragments arranged by size:
1429 835 262
Bsp1286I G_dGCh'C
Cuts at: 0 131 233 254 421 796 860 1694 1931
Size: 131 102 21 167 375 64 834 237
Cuts at: 1931 2526
Size: 595
Fragments arranged by size:
834 595 375 237 167 131 102 64
21
BspEI T'CCGG_A
Cuts at: 0 1526 2526
Size: 1526 1000
BspHI T'CATG_A
Cuts at: 0 1448 2381 2526
Size: 1448 933 145
Fragments arranged by size:
1448 933 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 814 967 996 1243 2189 2288 2526
Size: 814 153 29 247 946 99 238
Fragments arranged by size:
946 814 247 238 153 99 29
BsrDI GCAATG_nn'
Cuts at: 0 576 1238 2526
Size: 576 662 1288
Fragments arranged by size:
1288 662 576
BsrFI r'CCGG_y
Cuts at: 0 51 221 427 1532 1629 1735 2526
Size: 51 170 206 1105 97 106 791
Fragments arranged by size:
1105 791 206 170 106 97 51
BsrGI T'GTAC_A
Cuts at: 0 917 2526
Size: 917 1609
BssSI C'ACGA_G
Cuts at: 0 976 2526
Size: 976 1550
BstEII G'GTnAC_C
Cuts at: 0 655 930 2526
Size: 655 275 1596
Fragments arranged by size:
1596 655 275
BstXI CCAn_nnnn'nTGG
Cuts at: 0 842 945 1617 2526
Size: 842 103 672 909
Fragments arranged by size:
909 842 672 103
BstYI r'GATC_y
Cuts at: 0 1412 2162 2526
Size: 1412 750 364
Fragments arranged by size:
1412 750 364
Bsu36I CC'TnA_GG
Cuts at: 0 826 2526
Size: 826 1700
BtgI C'CryG_G
Cuts at: 0 422 782 816 1433 1917 1937 2300 2526
Size: 422 360 34 617 484 20 363 226
Fragments arranged by size:
617 484 422 363 360 226 34 20
BtsI GCAGTG_nn'
Cuts at: 0 31 1209 2526
Size: 31 1178 1317
Fragments arranged by size:
1317 1178 31
DraIII CAC_nnn'GTG
Cuts at: 0 655 1869 2526
Size: 655 1214 657
Fragments arranged by size:
1214 657 655
EaeI y'GGCC_r
Cuts at: 0 425 573 972 1737 2526
Size: 425 148 399 765 789
Fragments arranged by size:
789 765 425 399 148
EarI CTCTTCn'nnn_
Cuts at: 0 147 1288 2526
Size: 147 1141 1238
Fragments arranged by size:
1238 1141 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 448 631 1082 1696 2311 2341 2526
Size: 289 159 183 451 614 615 30 185
Fragments arranged by size:
615 614 451 289 185 183 159 30
EcoO109I rG'GnC_Cy
Cuts at: 0 621 1671 2526
Size: 621 1050 855
Fragments arranged by size:
1050 855 621
EcoRV GAT'ATC
Cuts at: 0 1272 2526
Size: 1272 1254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 655 2526
Size: 655 1871
HaeII r_GCGC'y
Cuts at: 0 528 2097 2223 2526
Size: 528 1569 126 303
Fragments arranged by size:
1569 528 303 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 499 531 1261 1293 1694 1726 2000 2032
Size: 499 32 730 32 401 32 274 32
Cuts at: 2032 2396 2428 2526
Size: 364 32 98
Fragments arranged by size:
730 499 401 364 274 98 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1581 2022 2526
Size: 1581 441 504
Fragments arranged by size:
1581 504 441
HindIII A'AGCT_T
Cuts at: 0 135 2526
Size: 135 2391
KpnI G_GTAC'C
Cuts at: 0 2372 2526
Size: 2372 154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 2526
Size: 1397 1129
MscI TGG'CCA
Cuts at: 0 575 2526
Size: 575 1951
MslI CAynn'nnrTG
Cuts at: 0 928 943 1102 1543 1615 1916 2506 2526
Size: 928 15 159 441 72 301 590 20
Fragments arranged by size:
928 590 441 301 159 72 20 15
MspA1I CmG'CkG
Cuts at: 0 984 2013 2116 2526
Size: 984 1029 103 410
Fragments arranged by size:
1029 984 410 103
NaeI GCC'GGC
Cuts at: 0 53 429 1631 2526
Size: 53 376 1202 895
Fragments arranged by size:
1202 895 376 53
NcoI C'CATG_G
Cuts at: 0 422 816 1917 1937 2526
Size: 422 394 1101 20 589
Fragments arranged by size:
1101 589 422 394 20
NgoMIV G'CCGG_C
Cuts at: 0 51 427 1629 2526
Size: 51 376 1202 897
Fragments arranged by size:
1202 897 376 51
NspI r_CATG'y
Cuts at: 0 278 607 1359 2319 2526
Size: 278 329 752 960 207
Fragments arranged by size:
960 752 329 278 207
PasI CC'CwG_GG
Cuts at: 0 2360 2526
Size: 2360 166
PciI A'CATG_T
Cuts at: 0 1355 2526
Size: 1355 1171
PflMI CCAn_nnn'nTGG
Cuts at: 0 267 1401 1869 1938 2526
Size: 267 1134 468 69 588
Fragments arranged by size:
1134 588 468 267 69
PfoI T'CCnGG_A
Cuts at: 0 872 2288 2526
Size: 872 1416 238
Fragments arranged by size:
1416 872 238
PmlI CAC'GTG
Cuts at: 0 416 2136 2526
Size: 416 1720 390
Fragments arranged by size:
1720 416 390
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 970 1002 2422 2454 2526
Size: 970 32 1420 32 72
Fragments arranged by size:
1420 970 72 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 621 1671 2526
Size: 621 1050 855
Fragments arranged by size:
1050 855 621
PshAI GACnn'nnGTC
Cuts at: 0 997 2526
Size: 997 1529
PstI C_TGCA'G
Cuts at: 0 211 479 1007 1518 1860 2115 2526
Size: 211 268 528 511 342 255 411
Fragments arranged by size:
528 511 411 342 268 255 211
PvuII CAG'CTG
Cuts at: 0 984 2013 2116 2526
Size: 984 1029 103 410
Fragments arranged by size:
1029 984 410 103
SalI G'TCGA_C
Cuts at: 0 1579 2526
Size: 1579 947
SapI GCTCTTCn'nnn_
Cuts at: 0 1288 2526
Size: 1288 1238
SbfI CC_TGCA'GG
Cuts at: 0 1007 2526
Size: 1007 1519
SexAI A'CCwGG_T
Cuts at: 0 651 2526
Size: 651 1875
SfcI C'TryA_G
Cuts at: 0 207 475 1003 1514 1603 1856 2111 2526
Size: 207 268 528 511 89 253 255 415
Fragments arranged by size:
528 511 415 268 255 253 207 89
SmlI C'TyrA_G
Cuts at: 0 1894 2526
Size: 1894 632
SphI G_CATG'C
Cuts at: 0 278 2319 2526
Size: 278 2041 207
Fragments arranged by size:
2041 278 207
StuI AGG'CCT
Cuts at: 0 1340 2526
Size: 1340 1186
StyI C'CwwG_G
Cuts at: 0 422 816 926 1917 1937 2526
Size: 422 394 110 991 20 589
Fragments arranged by size:
991 589 422 394 110 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 91 1246 1963 2502 2506 2526
Size: 91 1155 717 539 4 20
Fragments arranged by size:
1155 717 539 91 20 4
TatI w'GTAC_w
Cuts at: 0 917 2526
Size: 917 1609
Tth111I GACn'n_nGTC
Cuts at: 0 760 1706 2026 2526
Size: 760 946 320 500
Fragments arranged by size:
946 760 500 320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 14 780 839 942 1251 1825 2307 2357
Size: 14 766 59 103 309 574 482 50
Cuts at: 2357 2526
Size: 169
Fragments arranged by size:
766 574 482 309 169 103 59 50
14
XmnI GAAnn'nnTTC
Cuts at: 0 202 2526
Size: 202 2324
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI AvaI BaeI BanI BanII
BbsI BbvCI BclI BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EarI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PstI PvuII SalI SapI
SbfI SexAI SfcI SmlI SphI StuI StyI TaqII
TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AclI AflII AhdI ApaI ApoI AscI AseI
AsiSI AvrII BamHI BbeI BcgI BciVI BfrBI BglI
BglII BmrI BmtI BplI BsaBI BsaHI BsiEI BsiWI
BsmBI BsrBI BssHII BstAPI BstBI BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI
FseI FspI FspAI HpaI KasI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PmeI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TstI XbaI XhoI XmaI ZraI