Restriction map for
pFN21AE3375
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII BamHI BbeI BbrPI
BcgI BclI BglII BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HpaI KasI KpnI KspI MluI
MroI NarI NdeI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SfiI
SfoI SfuI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AcsI 2800 GAATTC [GA]^AATT[CT]
ApoI 2800 GAATTC [GA]^AATT[CT]
AvrII 2223 CCTAGG C^CTAGG
BalI 1082 TGGCCA TGG^CCA
BbvCI 2122 CCTCAGC CCTCAGC(-5/-2)
BciVI 2030 GTATCC GTATCC(6/5)
BlnI 2223 CCTAGG C^CTAGG
BlpI 1061 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1061 GCTTAGC GC^T[ACGT]AGC
BsaBI 1428 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2004 GCATTC GAATGC(1/-1)
BspMI 258 GCAGGT ACCTGC(4/8)
CelII 1061 GCTTAGC GC^T[ACGT]AGC
DraIII 1694 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2309 GACGGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1604 CCATGG C^C[GA][CT]GG
EcoRI 2800 GAATTC G^AATTC
HindIII 2371 AAGCTT A^AGCTT
MamI 1428 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 779 CAATTG C^AATTG
MluNI 1082 TGGCCA TGG^CCA
MscI 1082 TGGCCA TGG^CCA
MunI 779 CAATTG C^AATTG
NaeI 1523 GCCGGC GCC^GGC
NcoI 1604 CCATGG C^CATGG
NgoMIV 1523 GCCGGC G^CCGGC
NheI 3040 GCTAGC G^CTAGC
SbfI 1263 CCTGCAGG CCTGCA^GG
SgrAI 2665 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 1263 CCTGCAGG CCTGCA^GG
XcmI 641 CCAACCTCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
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AflII 805 CTTAAG C^TTAAG
2692 CTTAAG
AhdI 802 GACCTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1806 GACGCTTGGTC
AlwNI 354 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1408 CAGGAGCTG
Aor51HI 15 AGCGCT AGC^GCT
2588 AGCGCT
AspEI 802 GACCTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1806 GACGCTTGGTC
BbsI 1147 GAAGAC GAAGAC(2/6)
2830 GTCTTC
BfrI 805 CTTAAG C^TTAAG
2692 CTTAAG
BglI 43 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
362 GCCAGCCCGGC
BpuAI 1147 GAAGAC GAAGAC(2/6)
2830 GTCTTC
BsaAI 328 TACGTG [CT]AC^GT[GA]
1116 TACGTG
BsiEI 173 CGGCCG CG[GA][CT]^CG
2911 CGGCCG
BsmBI 2809 CGTCTC CGTCTC(1/5)
3010 GAGACG
BsrDI 609 CATTGC GCAATG(2/0)
2732 GCAATG
BstYI 2601 AGATCC [GA]^GATC[CT]
3221 AGATCC
EagI 173 CGGCCG C^GGCCG
2911 CGGCCG
Eam1105I 802 GACCTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1806 GACGCTTGGTC
EclXI 173 CGGCCG C^GGCCG
2911 CGGCCG
Eco47III 15 AGCGCT AGC^GCT
2588 AGCGCT
Eco52I 173 CGGCCG C^GGCCG
2911 CGGCCG
HincII 1689 GTTGAC GT[CT]^[GA]AC
2101 GTCAAC
HindII 1689 GTTGAC GT[CT]^[GA]AC
2101 GTCAAC
MflI 2601 AGATCC [GA]^GATC[CT]
3221 AGATCC
MseI 806 TTAA T^TAA
2693 TTAA
MslI 720 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
912 CACCAAGATG
PpuMI 377 AGGACCC [GA]G^G[AT]CC[CT]
675 AGGACCT
PstI 1264 CTGCAG CTGCA^G
1366 CTGCAG
SapI 1518 GAAGAGC GCTCTTC(1/4)
579 GCTCTTC
SexAI 1695 ACCAGGT A^CC[AT]GGT
1895 ACCTGGT
Tru9I 806 TTAA T^TAA
2693 TTAA
XhoII 2601 AGATCC [GA]^GATC[CT]
3221 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XmaCI XmaI