Restriction map for
pFN21AE3375
(Circular) (Six-base) MAPSORT of: pFN21AE3375.seq Check: 8821 from: 1 to: 7948
pFN21AE3375 7948 bp
With 182 enzymes: SgfI *
October 11, 2013 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2254 2254
Size: 7948
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5758 278
Size: 53 83 186 936 4222 2468
Fragments arranged by size:
4222 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5291 1937
Size: 3354 4594
Acc65I G'GTAC_C
Cuts at: 5269 5269
Size: 7948
AclI AA'CG_TT
Cuts at: 1563 6075 6448 1563
Size: 4512 373 3063
Fragments arranged by size:
4512 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 2657 2894 3554 6011 7395 7663 1632
Size: 986 39 237 660 2457 1384 268 1917
Fragments arranged by size:
2457 1917 1384 986 660 268 237 39
AfeI AGC'GCT
Cuts at: 2022 4595 2022
Size: 2573 5375
AflII C'TTAA_G
Cuts at: 820 1017 2810 4697 820
Size: 197 1793 1887 4071
Fragments arranged by size:
4071 1887 1793 197
AflIII A'CryG_T
Cuts at: 1203 6847 7824 1203
Size: 5644 977 1327
Fragments arranged by size:
5644 1327 977
AgeI A'CCGG_T
Cuts at: 6779 6779
Size: 7948
AhdI GACnn_n'nnGTC
Cuts at: 2812 3816 6677 2812
Size: 1004 2861 4083
Fragments arranged by size:
4083 2861 1004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7948
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7948
AlwNI CAG_nnn'CTG
Cuts at: 2364 3418 5691 7263 2364
Size: 1054 2273 1572 3049
Fragments arranged by size:
3049 2273 1572 1054
ApaI G_GGCC'C
Cuts at: 2138 3778 3914 4696 2138
Size: 1640 136 782 5390
Fragments arranged by size:
5390 1640 782 136
ApaLI G'TGCA_C
Cuts at: 6005 7161 6005
Size: 1156 6792
ApoI r'AATT_y
Cuts at: 1493 4805 5257 5483 5635 7744 1493
Size: 3312 452 226 152 2109 1697
Fragments arranged by size:
3312 2109 1697 452 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6502 160
Size: 872 5470 1606
Fragments arranged by size:
5470 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7948
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2898 3492 3835 4296 5273 1870
Size: 73 195 760 594 343 461 977 4545
Fragments arranged by size:
4545 977 760 594 461 343 195 73
AvrII C'CTAG_G
Cuts at: 4228 4228
Size: 7948
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4018 4051 4018
Size: 33 7915
BamHI G'GATC_C
Cuts at: 5278 5645 5278
Size: 367 7581
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2907 2953 3043 4163 5269
Size: 325 217 627 1120 46 90 1120 1106
Cuts at: 5269 6724 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1120 1120 1106 627 325 217
90 46
BanII G_rGCy'C
Cuts at: 1391 2013 2138 2436 2646 3456 3734 3778 3914
Size: 622 125 298 210 810 278 44 136
Cuts at: 3914 4098 4398 4696 4730 4751 5267 1391
Size: 184 300 298 34 21 516 4072
Fragments arranged by size:
4072 810 622 516 300 298 298 278
210 184 136 125 44 34 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3159 4828 928
Size: 971 1260 1669 4048
Fragments arranged by size:
4048 1669 1260 971
BbvCI CC'TCA_GC
Cuts at: 4128 4128
Size: 7948
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6139 6173 1903
Size: 34 4202 34 3678
Fragments arranged by size:
4202 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4046 5840 7050 4046
Size: 1794 1210 4944
Fragments arranged by size:
4944 1794 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7948
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2054 2373 6559
Size: 107 122 71 631 605 382 319 4186
Cuts at: 6559 136
Size: 1525
Fragments arranged by size:
4186 1525 631 605 382 319 122 107
71
BglII A'GATC_T
Cuts at: 7943 7943
Size: 7948
BlpI GC'TnA_GC
Cuts at: 3067 5365 3067
Size: 2298 5650
Bme1580I G_kGCm'C
Cuts at: 1790 2138 3048 3128 3778 3895 3914 4168 4696
Size: 348 910 80 650 117 19 254 528
Cuts at: 4696 6009 7165 1790
Size: 1313 1156 2573
Fragments arranged by size:
2573 1313 1156 910 650 528 348 254
117 80 19
BmgBI CAC'GTC
Cuts at: 1318 2236 3798 1318
Size: 918 1562 5468
Fragments arranged by size:
5468 1562 918
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2382 4097 4526
Size: 416 53 83 399 24 965 1715 429
Cuts at: 4526 4746 4776 5679 6637 442
Size: 220 30 903 958 1753
Fragments arranged by size:
1753 1715 965 958 903 429 416 399
220 83 53 30 24
BmtI G_CTAG'C
Cuts at: 1056 5049 1056
Size: 3993 3955
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2042 3850 4143 4483 2042
Size: 1808 293 340 5507
Fragments arranged by size:
5507 1808 340 293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 2341 2819 3628 4057 4295 4717 4779
Size: 760 342 478 809 429 238 422 62
Cuts at: 4779 4922 4990 6608 1239
Size: 143 68 1618 2579
Fragments arranged by size:
2579 1618 809 760 478 429 422 342
238 143 68 62
Bpu10I CC'TnA_GC
Cuts at: 2858 4128 4284 2858
Size: 1270 156 6522
Fragments arranged by size:
6522 1270 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3524 3836 4136 6073 6938 7236 3524
Size: 312 300 1937 865 298 4236
Fragments arranged by size:
4236 1937 865 312 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2847 4407 4788 4882 6611 882
Size: 617 1348 1560 381 94 1729 2219
Fragments arranged by size:
2219 1729 1560 1348 617 381 94
BsaAI yAC'GTr
Cuts at: 493 1204 2335 3123 5739 493
Size: 711 1131 788 2616 2702
Fragments arranged by size:
2702 2616 1131 788 711
BsaBI GATnn'nnATC
Cuts at: 3437 7942 3437
Size: 4505 3443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3403 4136 5018 5755
Size: 53 83 186 936 1870 733 882 737
Cuts at: 5755 6137 6772 275
Size: 382 635 1451
Fragments arranged by size:
1870 1451 936 882 737 733 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 6381 6779 7053 7200 7611 6381
Size: 398 274 147 411 6718
Fragments arranged by size:
6718 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2234 2264 3626 3656 5018
Size: 30 165 30 831 30 1362 30 1362
Cuts at: 5018 5048 5228 5258 1178
Size: 30 180 30 3868
Fragments arranged by size:
3868 1362 1362 831 180 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 2111 2114 2117 2120 2123 2126 2129
Size: 922 3 3 3 3 3 3 3
Cuts at: 2129 3239 3362 3365 3773 3776 3779 4106 4292
Size: 1110 123 3 408 3 3 327 186
Cuts at: 4292 4316 4406 4438 5081 1186
Size: 24 90 32 643 4053
Fragments arranged by size:
4053 1110 922 643 408 327 186 123
90 32 24 3 3 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2146 2227 2324 4016 4661 4787 4970 4993 5021
Size: 81 97 1692 645 126 183 23 28
Cuts at: 5021 7151 7647 2146
Size: 2130 496 2447
Fragments arranged by size:
2447 2130 1692 645 496 183 126 97
81 28 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2765 3351 3614 5206 1163
Size: 573 1029 586 263 1592 3905
Fragments arranged by size:
3905 1592 1029 586 573 263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2181 4919 5398 6159 7187 1540
Size: 275 186 180 2738 479 761 1028 2301
Fragments arranged by size:
2738 2301 1028 761 479 275 186 180
BsiHKAI G_wGCw'C
Cuts at: 2436 3456 3734 4358 6009 6094 7165 2436
Size: 1020 278 624 1651 85 1071 3219
Fragments arranged by size:
3219 1651 1071 1020 624 278 85
BsmI GAATG_Cn'
Cuts at: 4009 5459 5552 4009
Size: 1450 93 6405
Fragments arranged by size:
6405 1450 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4820 5009 4820
Size: 189 7759
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2138 2436 2646 3048 3128 3456
Size: 399 223 125 298 210 402 80 328
Cuts at: 3456 3734 3778 3895 3914 4098 4168 4358 4398
Size: 278 44 117 19 184 70 190 40
Cuts at: 4398 4696 4730 4751 5267 6009 6094 7165 1391
Size: 298 34 21 516 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 516 402 399 328 298
298 278 223 210 190 184 125 117
85 80 70 44 40 34 21 19
BspEI T'CCGG_A
Cuts at: 7611 7611
Size: 7948
BspHI T'CATG_A
Cuts at: 5837 7567 5837
Size: 1730 6218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2254 5303 844
Size: 1410 3049 3489
Fragments arranged by size:
3489 3049 1410
BsrBI CCG'CTC
Cuts at: 1394 1823 3427 3642 5071 5128 5192 5835 1394
Size: 429 1604 215 1429 57 64 643 3507
Fragments arranged by size:
3507 1604 1429 643 429 215 64 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 2613 4744 6443 6617 65
Size: 1183 1365 2131 1699 174 1396
Fragments arranged by size:
2131 1699 1396 1365 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3528 4671 4935 6592 6779 1700
Size: 110 1718 1143 264 1657 187 2869
Fragments arranged by size:
2869 1718 1657 1143 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7948
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7948
BssSI C'ACGA_G
Cuts at: 2673 3287 4047 4117 6008 7020 2673
Size: 614 760 70 1891 1012 3601
Fragments arranged by size:
3601 1891 1012 760 614 70
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7948
BstBI TT'CG_AA
Cuts at: 6752 6752
Size: 7948
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7948
BstXI CCAn_nnnn'nTGG
Cuts at: 3148 3952 4857 4900 5029 3148
Size: 804 905 43 129 6067
Fragments arranged by size:
6067 905 804 129 43
BstYI r'GATC_y
Cuts at: 1972 4606 5226 5278 5645 6030 6047 7499 7585
Size: 2634 620 52 367 385 17 1452 86
Cuts at: 7585 7597 7943 1972
Size: 12 346 1977
Fragments arranged by size:
2634 1977 1452 620 385 367 346 86
52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3609 513
Size: 552 939 1605 4852
Fragments arranged by size:
4852 1605 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4077 4512 487
Size: 683 56 2851 435 3923
Fragments arranged by size:
3923 2851 683 435 56
BtsI GCAGTG_nn'
Cuts at: 2998 3262 3368 3884 5464 6258 6278 2998
Size: 264 106 516 1580 794 20 4668
Fragments arranged by size:
4668 1580 794 516 264 106 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7948
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7913
DraI TTT'AAA
Cuts at: 5252 5252
Size: 7948
DraIII CAC_nnn'GTG
Cuts at: 3704 3704
Size: 7948
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4320 6955 809
Size: 507 3004 2635 1802
Fragments arranged by size:
3004 2635 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 3087 3420
Size: 54 1425 50 275 18 348 909 333
Cuts at: 3420 4113 4916 5395 6284 7913 8
Size: 693 803 479 889 1629 43
Fragments arranged by size:
1629 1425 909 889 803 693 479 348
333 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2178 4916 5395 1537
Size: 275 366 2738 479 4090
Fragments arranged by size:
4090 2738 479 366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2439 2591 3518 3617 3764 5878 1353
Size: 1086 152 927 99 147 2114 3423
Fragments arranged by size:
3423 2114 1086 927 152 147 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4690 6535 6909 7055 387
Size: 1064 138 3101 1845 374 146 1280
Fragments arranged by size:
3101 1845 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2434 3454 3732 2434
Size: 1020 278 6650
Fragments arranged by size:
6650 1020 278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2341 2618 2657 2819 2894 3554
Size: 393 367 342 277 39 162 75 660
Cuts at: 3554 3628 4057 4295 4717 4779 4922 4990 6011
Size: 74 429 238 422 62 143 68 1021
Cuts at: 6011 6608 7395 7663 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1021 787 660 597 429 422 393
367 342 277 268 238 162 143 75
74 68 62 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2837 3724 3905 4761 2837
Size: 887 181 856 6024
Fragments arranged by size:
6024 887 856 181
EcoO109I rG'GnC_Cy
Cuts at: 2135 2383 2681 3774 3775 3910 4540 4692 4693
Size: 248 298 1093 1 135 630 152 1
Cuts at: 4693 2135
Size: 5390
Fragments arranged by size:
5390 1093 630 298 248 152 135 1
1
EcoRI G'AATT_C
Cuts at: 4805 5257 5635 4805
Size: 452 378 7118
Fragments arranged by size:
7118 452 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3274 3274
Size: 7948
FspI TGC'GCA
Cuts at: 6454 6454
Size: 7948
HaeII r_GCGC'y
Cuts at: 1678 2024 2046 2352 4597 4858 7095 1678
Size: 346 22 306 2245 261 2237 2531
Fragments arranged by size:
2531 2245 2237 346 306 261 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2266 2798 2830 3161
Size: 32 135 32 662 32 532 32 331
Cuts at: 3161 3193 3802 3834 4055 4087 4738 4770 5228
Size: 32 609 32 221 32 651 32 458
Cuts at: 5228 5260 6589 6621 6663 6695 1373
Size: 32 1329 32 42 32 2626
Fragments arranged by size:
2626 1329 662 651 609 532 458 331
221 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3696 4108 5292 5538 669
Size: 988 281 1758 412 1184 246 3079
Fragments arranged by size:
3079 1758 1184 988 412 281 246
HindIII A'AGCT_T
Cuts at: 748 4376 748
Size: 3628 4320
HpaI GTT'AAC
Cuts at: 5538 5538
Size: 7948
KpnI G_GTAC'C
Cuts at: 5273 5273
Size: 7948
MfeI C'AATT_G
Cuts at: 2784 5547 2784
Size: 2763 5185
MluI A'CGCG_T
Cuts at: 7824 7824
Size: 7948
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4331 4515 7062 7246 1305
Size: 3026 184 2547 184 2007
Fragments arranged by size:
3026 2547 2007 184 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3089 10
Size: 54 1425 343 1257 4869
Fragments arranged by size:
4869 1425 1257 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2729 2921 5906 6265 6424 518
Size: 648 1563 192 2985 359 159 2042
Fragments arranged by size:
2985 2042 1563 648 359 192 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2164 2299 2308 2364 3475 4132 4179
Size: 445 113 135 9 56 1111 657 47
Cuts at: 4179 4264 5113 5182 5364 6041 7189 7434 1606
Size: 85 849 69 182 677 1148 245 2120
Fragments arranged by size:
2120 1148 1111 849 677 657 445 245
182 135 113 85 69 56 47 9
NaeI GCC'GGC
Cuts at: 3530 3530
Size: 7948
NcoI C'CATG_G
Cuts at: 513 1065 2004 3609 513
Size: 552 939 1605 4852
Fragments arranged by size:
4852 1605 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7948
NgoMIV G'CCGG_C
Cuts at: 3528 3528
Size: 7948
NheI G'CTAG_C
Cuts at: 1052 5045 1052
Size: 3993 3955
NotI GC'GGCC_GC
Cuts at: 5395 5395
Size: 7948
NspI r_CATG'y
Cuts at: 1132 1229 5306 6851 1132
Size: 97 4077 1545 2229
Fragments arranged by size:
4077 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3983 1348
Size: 2635 5313
PciI A'CATG_T
Cuts at: 6847 6847
Size: 7948
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 2842 3989 1108
Size: 1203 531 1147 5067
Fragments arranged by size:
5067 1203 1147 531
PfoI T'CCnGG_A
Cuts at: 3201 3441 4756 5136 3201
Size: 240 1315 380 6013
Fragments arranged by size:
6013 1315 380 240
PmeI GTTT'AAAC
Cuts at: 5252 5252
Size: 7948
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3438 3470 6017 6049 7530 7562 3438
Size: 32 2547 32 1481 32 3824
Fragments arranged by size:
3824 2547 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2383 2681 2383
Size: 298 7650
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7948
PsiI TTA'TAA
Cuts at: 5518 5518
Size: 7948
PspOMI G'GGCC_C
Cuts at: 2134 3774 3910 4692 2134
Size: 1640 136 782 5390
Fragments arranged by size:
5390 1640 782 136
PstI C_TGCA'G
Cuts at: 830 3273 3375 5300 830
Size: 2443 102 1925 3478
Fragments arranged by size:
3478 2443 1925 102
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7948
PvuII CAG'CTG
Cuts at: 2299 3475 4132 4264 2299
Size: 1176 657 132 5983
Fragments arranged by size:
5983 1176 657 132
SacI G_AGCT'C
Cuts at: 2436 3456 3734 2436
Size: 1020 278 6650
Fragments arranged by size:
6650 1020 278
SalI G'TCGA_C
Cuts at: 1936 5290 1936
Size: 3354 4594
SapI GCTCTTCn'nnn_
Cuts at: 2591 3518 2591
Size: 927 7021
SbfI CC_TGCA'GG
Cuts at: 3273 5300 3273
Size: 2027 5921
ScaI AGT'ACT
Cuts at: 6196 7908 6196
Size: 1712 6236
SexAI A'CCwGG_T
Cuts at: 3700 3900 3700
Size: 200 7748
SfcI C'TryA_G
Cuts at: 826 1045 3269 3371 4004 5061 5296 6431 7112
Size: 219 2224 102 633 1057 235 1135 681
Cuts at: 7112 7303 826
Size: 191 1471
Fragments arranged by size:
2224 1471 1135 1057 681 633 235 219
191 102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7948
SgrAI Cr'CCGG_yG
Cuts at: 1700 4671 1700
Size: 2971 4977
SmaI CCC'GGG
Cuts at: 1872 2140 3494 4298 5275 1872
Size: 268 1354 804 977 4545
Fragments arranged by size:
4545 1354 977 804 268
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2810 3539 3851 4151 4697 6052
Size: 197 926 867 729 312 300 546 1355
Cuts at: 6052 6953 7215 820
Size: 901 262 1553
Fragments arranged by size:
1553 1355 926 901 867 729 546 312
300 262 197
SnaBI TAC'GTA
Cuts at: 493 5739 493
Size: 5246 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7948
SphI G_CATG'C
Cuts at: 1132 5306 1132
Size: 4174 3774
SspI AAT'ATT
Cuts at: 5 52 5872 5
Size: 47 5820 2081
Fragments arranged by size:
5820 2081 47
StuI AGG'CCT
Cuts at: 1517 3599 3849 4227 5212 1517
Size: 2082 250 378 985 4253
Fragments arranged by size:
4253 2082 985 378 250
StyI C'CwwG_G
Cuts at: 513 1065 2004 2253 2439 3249 3609 3914 4228
Size: 552 939 249 186 810 360 305 314
Cuts at: 4228 4850 4881 4952 5387 513
Size: 622 31 71 435 3074
Fragments arranged by size:
3074 939 810 622 552 435 360 314
305 249 186 71 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4030 4646 5975 5992 6145 6330 6775
Size: 1255 2450 616 1329 17 153 185 445
Cuts at: 6775 325
Size: 1498
Fragments arranged by size:
2450 1498 1329 1255 616 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6194 7906 96
Size: 274 80 33 51 1443 4217 1712 138
Fragments arranged by size:
4217 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3655 3687 4940 4972 7908 7940 3655
Size: 32 1253 32 2936 32 3663
Fragments arranged by size:
3663 2936 1253 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7948
XbaI T'CTAG_A
Cuts at: 5284 5284
Size: 7948
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2653 2653
Size: 7948
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7948
XmaI C'CCGG_G
Cuts at: 1870 2138 3492 4296 5273 1870
Size: 268 1354 804 977 4545
Fragments arranged by size:
4545 1354 977 804 268
XmnI GAAnn'nnTTC
Cuts at: 6077 6077
Size: 7948
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5756 276
Size: 53 83 186 936 4222 2468
Fragments arranged by size:
4222 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI