Restriction map for
pFN21AE3375
(Linear) (Six-base) MAPSORT of: KIEE3375.seq Check: 7145 from: 1 to: 3243
KIEE3375.orf
With 182 enzymes: SgfI *
October 11, 2013 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 249 3243
Size: 249 2994
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 652 889 1549 3243
Size: 613 39 237 660 1694
Fragments arranged by size:
1694 660 613 237 39
AfeI AGC'GCT
Cuts at: 0 17 2590 3243
Size: 17 2573 653
Fragments arranged by size:
2573 653 17
AflII C'TTAA_G
Cuts at: 0 805 2692 3243
Size: 805 1887 551
Fragments arranged by size:
1887 805 551
AhdI GACnn_n'nnGTC
Cuts at: 0 807 1811 3243
Size: 807 1004 1432
Fragments arranged by size:
1432 1004 807
AlwNI CAG_nnn'CTG
Cuts at: 0 359 1413 3243
Size: 359 1054 1830
Fragments arranged by size:
1830 1054 359
ApaI G_GGCC'C
Cuts at: 0 133 1773 1909 2691 3243
Size: 133 1640 136 782 552
Fragments arranged by size:
1640 782 552 136 133
ApoI r'AATT_y
Cuts at: 0 2800 3243
Size: 2800 443
AvaI C'yCGr_G
Cuts at: 0 133 893 1487 1830 2291 3243
Size: 133 760 594 343 461 952
Fragments arranged by size:
952 760 594 461 343 133
AvrII C'CTAG_G
Cuts at: 0 2223 3243
Size: 2223 1020
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2013 2046 3243
Size: 2013 33 1197
Fragments arranged by size:
2013 1197 33
BanI G'GyrC_C
Cuts at: 0 902 948 1038 2158 3243
Size: 902 46 90 1120 1085
Fragments arranged by size:
1120 1085 902 90 46
BanII G_rGCy'C
Cuts at: 0 8 133 431 641 1451 1729 1773 1909
Size: 8 125 298 210 810 278 44 136
Cuts at: 1909 2093 2393 2691 2725 2746 3243
Size: 184 300 298 34 21 497
Fragments arranged by size:
810 497 300 298 298 278 210 184
136 125 44 34 21 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1154 2823 3243
Size: 1154 1669 420
Fragments arranged by size:
1669 1154 420
BbvCI CC'TCA_GC
Cuts at: 0 2123 3243
Size: 2123 1120
BciVI GTATCCnnnnn_n'
Cuts at: 0 2041 3243
Size: 2041 1202
BglI GCCn_nnn'nGGC
Cuts at: 0 49 368 3243
Size: 49 319 2875
Fragments arranged by size:
2875 319 49
BlpI GC'TnA_GC
Cuts at: 0 1062 3243
Size: 1062 2181
Bme1580I G_kGCm'C
Cuts at: 0 133 1043 1123 1773 1890 1909 2163 2691
Size: 133 910 80 650 117 19 254 528
Cuts at: 2691 3243
Size: 552
Fragments arranged by size:
910 650 552 528 254 133 117 80
19
BmgBI CAC'GTC
Cuts at: 0 231 1793 3243
Size: 231 1562 1450
Fragments arranged by size:
1562 1450 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 377 2092 2521 2741 2771 3243
Size: 377 1715 429 220 30 472
Fragments arranged by size:
1715 472 429 377 220 30
BmtI G_CTAG'C
Cuts at: 0 3044 3243
Size: 3044 199
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 37 1845 2138 2478 3243
Size: 37 1808 293 340 765
Fragments arranged by size:
1808 765 340 293 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 814 1623 2052 2290 2712 2774 2917
Size: 336 478 809 429 238 422 62 143
Cuts at: 2917 2985 3243
Size: 68 258
Fragments arranged by size:
809 478 429 422 336 258 238 143
68 62
Bpu10I CC'TnA_GC
Cuts at: 0 853 2123 2279 3243
Size: 853 1270 156 964
Fragments arranged by size:
1270 964 853 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 1831 2131 3243
Size: 1519 312 300 1112
Fragments arranged by size:
1519 1112 312 300
BsaI GGTCTCn'nnnn_
Cuts at: 0 842 2402 2783 2877 3243
Size: 842 1560 381 94 366
Fragments arranged by size:
1560 842 381 366 94
BsaAI yAC'GTr
Cuts at: 0 330 1118 3243
Size: 330 788 2125
Fragments arranged by size:
2125 788 330
BsaBI GATnn'nnATC
Cuts at: 0 1432 3243
Size: 1432 1811
BsaHI Gr'CG_yC
Cuts at: 0 1398 2131 3013 3243
Size: 1398 733 882 230
Fragments arranged by size:
1398 882 733 230
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 1621 1651 3013 3043 3223 3243
Size: 229 30 1362 30 1362 30 180 20
Fragments arranged by size:
1362 1362 229 180 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 106 109 112 115 118 121 124
Size: 103 3 3 3 3 3 3 3
Cuts at: 124 1234 1357 1360 1768 1771 1774 2101 2287
Size: 1110 123 3 408 3 3 327 186
Cuts at: 2287 2311 2401 2433 3076 3243
Size: 24 90 32 643 167
Fragments arranged by size:
1110 643 408 327 186 167 123 103
90 32 24 3 3 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 141 222 319 2011 2656 2782 2965 2988
Size: 141 81 97 1692 645 126 183 23
Cuts at: 2988 3016 3243
Size: 28 227
Fragments arranged by size:
1692 645 227 183 141 126 97 81
28 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1346 1609 3201 3243
Size: 760 586 263 1592 42
Fragments arranged by size:
1592 760 586 263 42
BsiEI CG_ry'CG
Cuts at: 0 176 2914 3243
Size: 176 2738 329
Fragments arranged by size:
2738 329 176
BsiHKAI G_wGCw'C
Cuts at: 0 431 1451 1729 2353 3243
Size: 431 1020 278 624 890
Fragments arranged by size:
1020 890 624 431 278
BsmI GAATG_Cn'
Cuts at: 0 2004 3243
Size: 2004 1239
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2815 3004 3243
Size: 2815 189 239
Fragments arranged by size:
2815 239 189
Bsp1286I G_dGCh'C
Cuts at: 0 8 133 431 641 1043 1123 1451 1729
Size: 8 125 298 210 402 80 328 278
Cuts at: 1729 1773 1890 1909 2093 2163 2353 2393 2691
Size: 44 117 19 184 70 190 40 298
Cuts at: 2691 2725 2746 3243
Size: 34 21 497
Fragments arranged by size:
497 402 328 298 298 278 210 190
184 125 117 80 70 44 40 34
21 19 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 249 3243
Size: 249 2994
BsrBI CCG'CTC
Cuts at: 0 1422 1637 3066 3123 3187 3243
Size: 1422 215 1429 57 64 56
Fragments arranged by size:
1429 1422 215 64 57 56
BsrDI GCAATG_nn'
Cuts at: 0 608 2739 3243
Size: 608 2131 504
Fragments arranged by size:
2131 608 504
BsrFI r'CCGG_y
Cuts at: 0 1523 2666 2930 3243
Size: 1523 1143 264 313
Fragments arranged by size:
1523 1143 313 264
BssSI C'ACGA_G
Cuts at: 0 668 1282 2042 2112 3243
Size: 668 614 760 70 1131
Fragments arranged by size:
1131 760 668 614 70
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1143 1947 2852 2895 3024 3243
Size: 1143 804 905 43 129 219
Fragments arranged by size:
1143 905 804 219 129 43
BstYI r'GATC_y
Cuts at: 0 2601 3221 3243
Size: 2601 620 22
Fragments arranged by size:
2601 620 22
BtgI C'CryG_G
Cuts at: 0 1604 3243
Size: 1604 1639
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2072 2507 3243
Size: 2072 435 736
Fragments arranged by size:
2072 736 435
BtsI GCAGTG_nn'
Cuts at: 0 993 1257 1363 1879 3243
Size: 993 264 106 516 1364
Fragments arranged by size:
1364 993 516 264 106
DraIII CAC_nnn'GTG
Cuts at: 0 1699 3243
Size: 1699 1544
DrdI GACnn_nn'nnGTC
Cuts at: 0 2315 3243
Size: 2315 928
EaeI y'GGCC_r
Cuts at: 0 173 1082 1415 2108 2911 3243
Size: 173 909 333 693 803 332
Fragments arranged by size:
909 803 693 333 332 173
EagI C'GGCC_G
Cuts at: 0 173 2911 3243
Size: 173 2738 332
Fragments arranged by size:
2738 332 173
EarI CTCTTCn'nnn_
Cuts at: 0 434 586 1513 1612 1759 3243
Size: 434 152 927 99 147 1484
Fragments arranged by size:
1484 927 434 152 147 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2685 3243
Size: 2685 558
EcoICRI GAG'CTC
Cuts at: 0 429 1449 1727 3243
Size: 429 1020 278 1516
Fragments arranged by size:
1516 1020 429 278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 613 652 814 889 1549 1623 2052
Size: 336 277 39 162 75 660 74 429
Cuts at: 2052 2290 2712 2774 2917 2985 3243
Size: 238 422 62 143 68 258
Fragments arranged by size:
660 429 422 336 277 258 238 162
143 75 74 68 62 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 832 1719 1900 2756 3243
Size: 832 887 181 856 487
Fragments arranged by size:
887 856 832 487 181
EcoO109I rG'GnC_Cy
Cuts at: 0 130 378 676 1769 1770 1905 2535 2687
Size: 130 248 298 1093 1 135 630 152
Cuts at: 2687 2688 3243
Size: 1 555
Fragments arranged by size:
1093 630 555 298 248 152 135 130
1 1
EcoRI G'AATT_C
Cuts at: 0 2800 3243
Size: 2800 443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1269 3243
Size: 1269 1974
HaeII r_GCGC'y
Cuts at: 0 19 41 347 2592 2853 3243
Size: 19 22 306 2245 261 390
Fragments arranged by size:
2245 390 306 261 22 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 793 825 1156 1188 1797 1829
Size: 229 32 532 32 331 32 609 32
Cuts at: 1829 2050 2082 2733 2765 3223 3243
Size: 221 32 651 32 458 20
Fragments arranged by size:
651 609 532 458 331 229 221 32
32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 0 1691 2103 3243
Size: 1691 412 1140
Fragments arranged by size:
1691 1140 412
HindIII A'AGCT_T
Cuts at: 0 2371 3243
Size: 2371 872
MfeI C'AATT_G
Cuts at: 0 779 3243
Size: 779 2464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2326 2510 3243
Size: 2326 184 733
Fragments arranged by size:
2326 733 184
MscI TGG'CCA
Cuts at: 0 1084 3243
Size: 1084 2159
MslI CAynn'nnrTG
Cuts at: 0 724 916 3243
Size: 724 192 2327
Fragments arranged by size:
2327 724 192
MspA1I CmG'CkG
Cuts at: 0 46 159 294 303 359 1470 2127 2174
Size: 46 113 135 9 56 1111 657 47
Cuts at: 2174 2259 3108 3177 3243
Size: 85 849 69 66
Fragments arranged by size:
1111 849 657 135 113 85 69 66
56 47 46 9
NaeI GCC'GGC
Cuts at: 0 1525 3243
Size: 1525 1718
NcoI C'CATG_G
Cuts at: 0 1604 3243
Size: 1604 1639
NgoMIV G'CCGG_C
Cuts at: 0 1523 3243
Size: 1523 1720
NheI G'CTAG_C
Cuts at: 0 3040 3243
Size: 3040 203
PasI CC'CwG_GG
Cuts at: 0 1978 3243
Size: 1978 1265
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 837 1984 3243
Size: 306 531 1147 1259
Fragments arranged by size:
1259 1147 531 306
PfoI T'CCnGG_A
Cuts at: 0 1196 1436 2751 3131 3243
Size: 1196 240 1315 380 112
Fragments arranged by size:
1315 1196 380 240 112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1433 1465 3243
Size: 1433 32 1778
Fragments arranged by size:
1778 1433 32
PpuMI rG'GwC_Cy
Cuts at: 0 378 676 3243
Size: 378 298 2567
Fragments arranged by size:
2567 378 298
PspOMI G'GGCC_C
Cuts at: 0 129 1769 1905 2687 3243
Size: 129 1640 136 782 556
Fragments arranged by size:
1640 782 556 136 129
PstI C_TGCA'G
Cuts at: 0 1268 1370 3243
Size: 1268 102 1873
Fragments arranged by size:
1873 1268 102
PvuII CAG'CTG
Cuts at: 0 294 1470 2127 2259 3243
Size: 294 1176 657 132 984
Fragments arranged by size:
1176 984 657 294 132
SacI G_AGCT'C
Cuts at: 0 431 1451 1729 3243
Size: 431 1020 278 1514
Fragments arranged by size:
1514 1020 431 278
SapI GCTCTTCn'nnn_
Cuts at: 0 586 1513 3243
Size: 586 927 1730
Fragments arranged by size:
1730 927 586
SbfI CC_TGCA'GG
Cuts at: 0 1268 3243
Size: 1268 1975
SexAI A'CCwGG_T
Cuts at: 0 1695 1895 3243
Size: 1695 200 1348
Fragments arranged by size:
1695 1348 200
SfcI C'TryA_G
Cuts at: 0 1264 1366 1999 3056 3243
Size: 1264 102 633 1057 187
Fragments arranged by size:
1264 1057 633 187 102
SgrAI Cr'CCGG_yG
Cuts at: 0 2666 3243
Size: 2666 577
SmaI CCC'GGG
Cuts at: 0 135 1489 2293 3243
Size: 135 1354 804 950
Fragments arranged by size:
1354 950 804 135
SmlI C'TyrA_G
Cuts at: 0 805 1534 1846 2146 2692 3243
Size: 805 729 312 300 546 551
Fragments arranged by size:
805 729 551 546 312 300
StuI AGG'CCT
Cuts at: 0 1594 1844 2222 3207 3243
Size: 1594 250 378 985 36
Fragments arranged by size:
1594 985 378 250 36
StyI C'CwwG_G
Cuts at: 0 248 434 1244 1604 1909 2223 2845 2876
Size: 248 186 810 360 305 314 622 31
Cuts at: 2876 2947 3243
Size: 71 296
Fragments arranged by size:
810 622 360 314 305 296 248 186
71 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2025 2641 3243
Size: 2025 616 602
Fragments arranged by size:
2025 616 602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1650 1682 2935 2967 3243
Size: 1650 32 1253 32 276
Fragments arranged by size:
1650 1253 276 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 648 3243
Size: 648 2595
XmaI C'CCGG_G
Cuts at: 0 133 1487 2291 3243
Size: 133 1354 804 952
Fragments arranged by size:
1354 952 804 133
Enzymes that do cut:
AarI AcuI AfeI AflII AhdI AlwNI ApaI ApoI
AvaI AvrII BaeI BanI BanII BbsI BbvCI BciVI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NheI PasI PflMI PfoI PpiI PpuMI PspOMI
PstI PvuII SacI SapI SbfI SexAI SfcI SgrAI
SmaI SmlI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflIII AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI BamHI BbeI BcgI
BclI BfrBI BglII BsaWI BsiWI BspEI BspHI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI EcoRV FseI FspI FspAI HpaI KasI KpnI
MluI NarI NdeI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI ScaI SfiI SfoI SgfI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI