(Circular) (Six-base) MAPSORT of: pFN21AE4296.seq Check: 6605 from: 1 to: 14125 pFN21AE4296 14125 bp With 182 enzymes: SgfI * August 14, 2013 14:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5439 10244 10332 10778 11242 11429 5439 Size: 4805 88 446 464 187 8135 Fragments arranged by size: 8135 4805 464 446 187 88 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4986 11935 278 Size: 53 83 186 936 3450 6949 2468 Fragments arranged by size: 6949 3450 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6191 6945 8022 11468 1937 Size: 4254 754 1077 3446 4594 Fragments arranged by size: 4594 4254 3446 1077 754 Acc65I G'GTAC_C Cuts at: 3752 11446 3752 Size: 7694 6431 AclI AA'CG_TT Cuts at: 1563 12252 12625 1563 Size: 10689 373 3063 Fragments arranged by size: 10689 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2795 3133 3426 3708 3832 4174 4463 4612 Size: 1163 338 293 282 124 342 289 149 Cuts at: 4612 5352 5456 5750 7083 7298 7904 7928 8111 Size: 740 104 294 1333 215 606 24 183 Cuts at: 8111 8403 10179 10797 12188 13572 13840 1632 Size: 292 1776 618 1391 1384 268 1917 Fragments arranged by size: 1917 1776 1391 1384 1333 1163 740 618 606 342 338 294 293 292 289 282 268 215 183 149 124 104 24 AflII C'TTAA_G Cuts at: 820 1017 8443 820 Size: 197 7426 6502 Fragments arranged by size: 7426 6502 197 AflIII A'CryG_T Cuts at: 1203 4771 6317 8259 9224 9226 11221 13024 14001 Size: 3568 1546 1942 965 2 1995 1803 977 Cuts at: 14001 1203 Size: 1327 Fragments arranged by size: 3568 1995 1942 1803 1546 1327 977 965 2 AgeI A'CCGG_T Cuts at: 12956 12956 Size: 14125 AhdI GACnn_n'nnGTC Cuts at: 2416 10843 12854 2416 Size: 8427 2011 3687 Fragments arranged by size: 8427 3687 2011 AleI CACnn'nnGTG Cuts at: 1166 9227 9241 11042 1166 Size: 8061 14 1801 4249 Fragments arranged by size: 8061 4249 1801 14 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4442 8565 10340 1184 Size: 3258 4123 1775 4969 Fragments arranged by size: 4969 4123 3258 1775 AlwNI CAG_nnn'CTG Cuts at: 2179 2810 5651 7391 7516 8339 8650 8683 10041 Size: 631 2841 1740 125 823 311 33 1358 Cuts at: 10041 10222 11065 11868 13440 2179 Size: 181 843 803 1572 2864 Fragments arranged by size: 2864 2841 1740 1572 1358 843 823 803 631 311 181 125 33 ApaI G_GGCC'C Cuts at: 5398 8671 11140 5398 Size: 3273 2469 8383 Fragments arranged by size: 8383 3273 2469 ApaLI G'TGCA_C Cuts at: 7990 8930 10187 10724 12182 13338 7990 Size: 940 1257 537 1458 1156 8777 Fragments arranged by size: 8777 1458 1257 1156 940 537 ApoI r'AATT_y Cuts at: 1493 2347 2357 3935 6546 7023 7198 8065 8463 Size: 854 10 1578 2611 477 175 867 398 Cuts at: 8463 8750 10547 11434 11660 11812 13921 1493 Size: 287 1797 887 226 152 2109 1697 Fragments arranged by size: 2611 2109 1797 1697 1578 887 867 854 477 398 287 226 175 152 10 AseI AT'TA_AT Cuts at: 160 1032 7131 12679 160 Size: 872 6099 5548 1606 Fragments arranged by size: 6099 5548 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 14125 AvaI C'yCGr_G Cuts at: 1870 1943 2501 3164 6160 8573 8664 9806 11450 Size: 73 558 663 2996 2413 91 1142 1644 Cuts at: 11450 1870 Size: 4545 Fragments arranged by size: 4545 2996 2413 1644 1142 663 558 91 73 AvrII C'CTAG_G Cuts at: 5878 8449 5878 Size: 2571 11554 BamHI G'GATC_C Cuts at: 7935 9730 10044 10932 11455 11822 7935 Size: 1795 314 888 523 367 10238 Fragments arranged by size: 10238 1795 888 523 367 314 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2531 2962 3752 4373 5401 Size: 325 217 627 744 431 790 621 1028 Cuts at: 5401 8531 9764 10225 11446 12901 618 Size: 3130 1233 461 1221 1455 1842 Fragments arranged by size: 3130 1842 1455 1233 1221 1028 790 744 627 621 461 431 325 217 BanII G_rGCy'C Cuts at: 1391 2508 3127 4681 5398 7095 7465 7693 8142 Size: 1117 619 1554 717 1697 370 228 449 Cuts at: 8142 8671 9461 10345 11140 11319 11444 1391 Size: 529 790 884 795 179 125 4072 Fragments arranged by size: 4072 1697 1554 1117 884 795 790 717 619 529 449 370 228 179 125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4446 4761 5081 5954 6209 6380 6804 Size: 971 2547 315 320 873 255 171 424 Cuts at: 6804 7263 8557 9477 10206 928 Size: 459 1294 920 729 4847 Fragments arranged by size: 4847 2547 1294 971 920 873 729 459 424 320 315 255 171 BbvCI CC'TCA_GC Cuts at: 2145 2154 2187 2241 4145 4337 2145 Size: 9 33 54 1904 192 11933 Fragments arranged by size: 11933 1904 192 54 33 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8012 8046 8122 8156 8564 8598 9920 Size: 34 6075 34 76 34 408 34 1322 Cuts at: 9920 9954 12316 12350 1903 Size: 34 2362 34 3678 Fragments arranged by size: 6075 3678 2362 1322 408 76 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5876 12017 13227 5876 Size: 6141 1210 6774 Fragments arranged by size: 6774 6141 1210 BclI T'GATC_A Cuts at: 1548 3970 4545 7473 7779 10086 1548 Size: 2422 575 2928 306 2307 5587 Fragments arranged by size: 5587 2928 2422 2307 575 306 BfrBI ATG'CAT Cuts at: 4897 4897 Size: 14125 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3003 3842 6617 Size: 107 122 71 631 605 1331 839 2775 Cuts at: 6617 9509 10748 12736 136 Size: 2892 1239 1988 1525 Fragments arranged by size: 2892 2775 1988 1525 1331 1239 839 631 605 122 107 71 BglII A'GATC_T Cuts at: 7774 14120 7774 Size: 6346 7779 BlpI GC'TnA_GC Cuts at: 2571 5803 9638 10060 11542 2571 Size: 3232 3835 422 1482 5154 Fragments arranged by size: 5154 3835 3232 1482 422 Bme1580I G_kGCm'C Cuts at: 1790 2536 2967 4376 5398 5406 5458 7994 8259 Size: 746 431 1409 1022 8 52 2536 265 Cuts at: 8259 8488 8671 8934 9234 9769 10191 10633 10728 Size: 229 183 263 300 535 422 442 95 Cuts at: 10728 11140 12186 13342 1790 Size: 412 1046 1156 2573 Fragments arranged by size: 2573 2536 1409 1156 1046 1022 746 535 442 431 422 412 300 265 263 229 183 95 52 8 BmgBI CAC'GTC Cuts at: 1318 5461 5473 9960 11260 1318 Size: 4143 12 4487 1300 4183 Fragments arranged by size: 4487 4183 4143 1300 12 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5102 5687 5971 Size: 416 53 83 399 24 3685 585 284 Cuts at: 5971 6190 7048 8080 8311 8700 10936 11020 11110 Size: 219 858 1032 231 389 2236 84 90 Cuts at: 11110 11856 12814 442 Size: 746 958 1753 Fragments arranged by size: 3685 2236 1753 1032 958 858 746 585 416 399 389 284 231 219 90 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 8435 1056 Size: 7379 6746 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3376 7709 7979 10687 3376 Size: 4333 270 2708 6814 Fragments arranged by size: 6814 4333 2708 270 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2337 3149 3294 3441 3583 6025 6164 7641 Size: 1098 812 145 147 142 2442 139 1477 Cuts at: 7641 7785 8208 8623 8807 8893 8963 8985 9302 Size: 144 423 415 184 86 70 22 317 Cuts at: 9302 9948 9958 10011 10319 10388 10664 11267 12785 Size: 646 10 53 308 69 276 603 1518 Cuts at: 12785 1239 Size: 2579 Fragments arranged by size: 2579 2442 1518 1477 1098 812 646 603 423 415 317 308 276 184 147 145 144 142 139 86 70 69 53 22 10 Bpu10I CC'TnA_GC Cuts at: 2145 2154 2187 2241 3219 4145 4337 8423 9173 Size: 9 33 54 978 926 192 4086 750 Cuts at: 9173 10310 2145 Size: 1137 5960 Fragments arranged by size: 5960 4086 1137 978 926 750 192 54 33 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2030 2824 3607 8789 10046 11366 12250 13115 13413 Size: 794 783 5182 1257 1320 884 865 298 Cuts at: 13413 2030 Size: 2742 Fragments arranged by size: 5182 2742 1320 1257 884 865 794 783 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2647 2669 4272 8698 8887 10123 12788 Size: 617 1148 22 1603 4426 189 1236 2665 Cuts at: 12788 882 Size: 2219 Fragments arranged by size: 4426 2665 2219 1603 1236 1148 617 189 22 BsaAI yAC'GTr Cuts at: 493 1204 4357 8260 9227 10483 11157 11916 493 Size: 711 3153 3903 967 1256 674 759 2702 Fragments arranged by size: 3903 3153 2702 1256 967 759 711 674 BsaBI GATnn'nnATC Cuts at: 2481 6399 7352 14119 2481 Size: 3918 953 6767 2487 Fragments arranged by size: 6767 3918 2487 953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4983 6114 6989 9933 Size: 53 83 186 936 3450 1131 875 2944 Cuts at: 9933 11932 12314 12949 275 Size: 1999 382 635 1451 Fragments arranged by size: 3450 2944 1999 1451 1131 936 875 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3119 4105 6940 7374 8281 12558 12956 13230 13377 Size: 986 2835 434 907 4277 398 274 147 Cuts at: 13377 13788 3119 Size: 411 3456 Fragments arranged by size: 4277 3456 2835 986 907 434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2533 2563 4149 4179 4894 Size: 30 165 30 1130 30 1586 30 715 Cuts at: 4894 4924 5113 5143 5957 5987 8897 8927 9989 Size: 30 189 30 814 30 2910 30 1062 Cuts at: 9989 10019 1178 Size: 30 5284 Fragments arranged by size: 5284 2910 1586 1130 1062 814 715 189 165 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2133 2258 3239 3590 4308 5381 6028 7643 Size: 947 125 981 351 718 1073 647 1615 Cuts at: 7643 8211 8875 9035 9038 9041 9467 9911 9932 Size: 568 664 160 3 3 426 444 21 Cuts at: 9932 9935 9938 10418 10613 11324 11327 1186 Size: 3 3 480 195 711 3 3984 Fragments arranged by size: 3984 1615 1073 981 947 718 711 664 647 568 480 444 426 351 195 160 125 21 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 5347 6002 9562 9817 10328 13328 13824 5347 Size: 655 3560 255 511 3000 496 5648 Fragments arranged by size: 5648 3560 3000 655 511 496 255 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2422 4017 5640 6155 6255 6344 Size: 573 501 185 1595 1623 515 100 89 Cuts at: 6344 7580 8325 8376 8558 8619 8978 9527 9924 Size: 1236 745 51 182 61 359 549 397 Cuts at: 9924 10331 11051 1163 Size: 407 720 4237 Fragments arranged by size: 4237 1623 1595 1236 745 720 573 549 515 501 407 397 359 185 182 100 89 61 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 11145 11323 11575 12336 13364 1540 Size: 275 186 9144 178 252 761 1028 2301 Fragments arranged by size: 9144 2301 1028 761 275 252 186 178 BsiHKAI G_wGCw'C Cuts at: 2508 2883 3330 4722 5059 5302 6025 7001 7095 Size: 375 447 1392 337 243 723 976 94 Cuts at: 7095 7743 7965 7994 8934 9531 10191 10307 10728 Size: 648 222 29 940 597 660 116 421 Cuts at: 10728 11319 12186 12271 13342 2508 Size: 591 867 85 1071 3291 Fragments arranged by size: 3291 1392 1071 976 940 867 723 660 648 597 591 447 421 375 337 243 222 116 94 85 29 BsmI GAATG_Cn' Cuts at: 2450 4419 6250 8398 9290 11636 11729 2450 Size: 1969 1831 2148 892 2346 93 4846 Fragments arranged by size: 4846 2346 2148 1969 1831 892 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4895 8192 9966 4895 Size: 3297 1774 9054 Fragments arranged by size: 9054 3297 1774 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2508 2536 2883 2967 3127 3330 4376 Size: 399 718 28 347 84 160 203 1046 Cuts at: 4376 4681 4722 5059 5302 5398 5406 5458 6025 Size: 305 41 337 243 96 8 52 567 Cuts at: 6025 7001 7095 7465 7693 7743 7965 7994 8142 Size: 976 94 370 228 50 222 29 148 Cuts at: 8142 8259 8488 8671 8934 9234 9461 9531 9769 Size: 117 229 183 263 300 227 70 238 Cuts at: 9769 10191 10307 10345 10633 10728 11140 11319 11444 Size: 422 116 38 288 95 412 179 125 Cuts at: 11444 12186 12271 13342 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1046 976 742 718 567 422 412 399 370 347 337 305 300 288 263 243 238 229 228 227 222 203 183 179 160 148 125 117 116 96 95 94 85 84 70 52 50 41 38 29 28 8 BspEI T'CCGG_A Cuts at: 4105 7374 8281 13788 4105 Size: 3269 907 5507 4442 Fragments arranged by size: 5507 4442 3269 907 BspHI T'CATG_A Cuts at: 3472 4500 5029 6880 8400 10977 12014 13744 3472 Size: 1028 529 1851 1520 2577 1037 1730 3853 Fragments arranged by size: 3853 2577 1851 1730 1520 1037 1028 529 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2971 3458 4332 5439 6328 10244 10332 10386 Size: 2127 487 874 1107 889 3916 88 54 Cuts at: 10386 10778 11242 11429 11480 844 Size: 392 464 187 51 3489 Fragments arranged by size: 3916 3489 2127 1107 889 874 487 464 392 187 88 54 51 BsrBI CCG'CTC Cuts at: 1394 1823 8529 9811 10867 12012 1394 Size: 429 6706 1282 1056 1145 3507 Fragments arranged by size: 6706 3507 1282 1145 1056 429 BsrDI GCAATG_nn' Cuts at: 65 1248 9167 9543 9684 12620 12794 65 Size: 1183 7919 376 141 2936 174 1396 Fragments arranged by size: 7919 2936 1396 1183 376 174 141 BsrFI r'CCGG_y Cuts at: 1700 1810 6616 8590 9151 9554 11379 12769 12956 Size: 110 4806 1974 561 403 1825 1390 187 Cuts at: 12956 1700 Size: 2869 Fragments arranged by size: 4806 2869 1974 1825 1390 561 403 187 110 BsrGI T'GTAC_A Cuts at: 96 4478 7121 10032 10776 96 Size: 4382 2643 2911 744 3445 Fragments arranged by size: 4382 3445 2911 2643 744 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 14125 BssSI C'ACGA_G Cuts at: 6214 7793 9437 9779 12185 13197 6214 Size: 1579 1644 342 2406 1012 7142 Fragments arranged by size: 7142 2406 1644 1579 1012 342 BstAPI GCAn_nnn'nTGC Cuts at: 794 3511 5297 10021 794 Size: 2717 1786 4724 4898 Fragments arranged by size: 4898 4724 2717 1786 BstBI TT'CG_AA Cuts at: 12929 12929 Size: 14125 BstEII G'GTnAC_C Cuts at: 1184 9258 11413 1184 Size: 8074 2155 3896 Fragments arranged by size: 8074 3896 2155 BstXI CCAn_nnnn'nTGG Cuts at: 2704 4745 5984 7619 9243 10924 2704 Size: 2041 1239 1635 1624 1681 5905 Fragments arranged by size: 5905 2041 1681 1635 1624 1239 BstYI r'GATC_y Cuts at: 1972 3529 5242 5761 5874 7774 7935 8802 8818 Size: 1557 1713 519 113 1900 161 867 16 Cuts at: 8818 9037 9300 9730 10044 10103 10206 10932 11455 Size: 219 263 430 314 59 103 726 523 Cuts at: 11455 11822 12207 12224 13676 13762 13774 14120 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1900 1713 1557 1452 867 726 523 519 430 385 367 346 314 263 219 161 113 103 86 59 17 16 12 Bsu36I CC'TnA_GG Cuts at: 2951 4233 5644 6122 9890 2951 Size: 1282 1411 478 3768 7186 Fragments arranged by size: 7186 3768 1411 1282 478 BtgI C'CryG_G Cuts at: 513 1065 2004 3494 3633 3861 4221 4854 5406 Size: 552 939 1490 139 228 360 633 552 Cuts at: 5406 9306 10272 10743 11040 11088 11148 11198 513 Size: 3900 966 471 297 48 60 50 3440 Fragments arranged by size: 3900 3440 1490 966 939 633 552 552 471 360 297 228 139 60 50 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2362 487 Size: 683 56 1136 12250 Fragments arranged by size: 12250 1136 683 56 BtsI GCAGTG_nn' Cuts at: 2410 2826 3509 3901 3931 4299 4879 5097 5614 Size: 416 683 392 30 368 580 218 517 Cuts at: 5614 5843 6785 7331 7417 8342 8961 9066 10389 Size: 229 942 546 86 925 619 105 1323 Cuts at: 10389 10453 10500 11641 12435 12455 2410 Size: 64 47 1141 794 20 4080 Fragments arranged by size: 4080 1323 1141 942 925 794 683 619 580 546 517 416 392 368 229 218 105 86 64 47 30 20 ClaI AT'CG_AT Cuts at: 1554 4269 4551 1554 Size: 2715 282 11128 Fragments arranged by size: 11128 2715 282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 8878 8913 9394 9429 529 Size: 35 8314 35 481 35 5225 Fragments arranged by size: 8314 5225 481 35 35 35 DraI TTT'AAA Cuts at: 4701 6297 11429 4701 Size: 1596 5132 7397 Fragments arranged by size: 7397 5132 1596 DraIII CAC_nnn'GTG Cuts at: 5156 8929 9862 10459 5156 Size: 3773 933 597 8822 Fragments arranged by size: 8822 3773 933 597 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 13132 809 Size: 507 11816 1802 Fragments arranged by size: 11816 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3235 3534 4851 Size: 54 1425 50 275 18 1405 299 1317 Cuts at: 4851 5196 5274 5375 5838 6582 7029 8473 9501 Size: 345 78 101 463 744 447 1444 1028 Cuts at: 9501 9552 9841 10077 10926 11034 11151 11572 12461 Size: 51 289 236 849 108 117 421 889 Cuts at: 12461 14090 8 Size: 1629 43 Fragments arranged by size: 1629 1444 1425 1405 1317 1028 889 849 744 463 447 421 345 299 289 275 236 117 108 101 78 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 11572 1537 Size: 275 9760 4090 Fragments arranged by size: 9760 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3152 3862 5171 5426 5865 7620 7678 7706 Size: 1799 710 1309 255 439 1755 58 28 Cuts at: 7706 7923 8236 8474 9909 12055 1353 Size: 217 313 238 1435 2146 3423 Fragments arranged by size: 3423 2146 1799 1755 1435 1309 710 439 313 255 238 217 58 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2815 3533 4043 4427 6155 10292 Size: 1064 138 1226 718 510 384 1728 4137 Cuts at: 10292 12712 13086 13232 387 Size: 2420 374 146 1280 Fragments arranged by size: 4137 2420 1728 1280 1226 1064 718 510 384 374 146 138 EcoICRI GAG'CTC Cuts at: 2506 7093 11317 2506 Size: 4587 4224 5314 Fragments arranged by size: 5314 4587 4224 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2337 2795 3133 3149 3294 3426 3441 Size: 393 705 458 338 16 145 132 15 Cuts at: 3441 3583 3708 3832 4174 4463 4612 5352 5456 Size: 142 125 124 342 289 149 740 104 Cuts at: 5456 5750 6025 6164 7083 7298 7641 7785 7904 Size: 294 275 139 919 215 343 144 119 Cuts at: 7904 7928 8111 8208 8403 8623 8807 8893 8963 Size: 24 183 97 195 220 184 86 70 Cuts at: 8963 8985 9302 9948 9958 10011 10179 10319 10388 Size: 22 317 646 10 53 168 140 69 Cuts at: 10388 10664 10797 11267 12188 12785 13572 13840 1239 Size: 276 133 470 921 597 787 268 1524 Fragments arranged by size: 1524 921 919 787 740 705 646 597 470 458 393 343 342 338 317 294 289 276 275 268 220 215 195 184 183 168 149 145 144 142 140 139 133 132 125 124 119 104 97 86 70 69 53 24 22 16 15 10 EcoNI CCTnn'n_nnAGG Cuts at: 3872 5435 9893 3872 Size: 1563 4458 8104 Fragments arranged by size: 8104 4458 1563 EcoO109I rG'GnC_Cy Cuts at: 3651 3701 4135 4317 4344 5395 5648 6103 8668 Size: 50 434 182 27 1051 253 455 2565 Cuts at: 8668 8773 9483 10071 11136 11137 11328 3651 Size: 105 710 588 1065 1 191 6448 Fragments arranged by size: 6448 2565 1065 1051 710 588 455 434 253 191 182 105 50 27 1 EcoRI G'AATT_C Cuts at: 6546 7023 11434 11812 6546 Size: 477 4411 378 8859 Fragments arranged by size: 8859 4411 477 378 EcoRV GAT'ATC Cuts at: 3583 7017 3583 Size: 3434 10691 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4229 5518 5971 8225 9349 4229 Size: 1289 453 2254 1124 9005 Fragments arranged by size: 9005 2254 1289 1124 453 FspI TGC'GCA Cuts at: 12631 12631 Size: 14125 HaeII r_GCGC'y Cuts at: 1678 3615 6031 10651 13272 1678 Size: 1937 2416 4620 2621 2531 Fragments arranged by size: 4620 2621 2531 2416 1937 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 2900 2932 3070 Size: 32 135 32 830 32 466 32 138 Cuts at: 3070 3102 3350 3363 3382 3395 3821 3853 4013 Size: 32 248 13 19 13 426 32 160 Cuts at: 4013 4045 4894 4926 5674 5706 5975 6007 8762 Size: 32 849 32 748 32 269 32 2755 Cuts at: 8762 8794 8803 8835 9984 9989 10016 10021 10198 Size: 32 9 32 1149 5 27 5 177 Cuts at: 10198 10230 10673 10705 10829 10856 10861 10888 10988 Size: 32 443 32 124 27 5 27 100 Cuts at: 10988 11020 12766 12798 12840 12872 1373 Size: 32 1746 32 42 32 2626 Fragments arranged by size: 2755 2626 1746 1149 849 830 748 466 443 426 269 248 177 160 138 135 124 100 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 27 27 27 19 13 13 9 5 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 7059 7528 8023 9214 9970 9997 Size: 988 281 5121 469 495 1191 756 27 Cuts at: 9997 11469 11715 669 Size: 1472 246 3079 Fragments arranged by size: 5121 3079 1472 1191 988 756 495 469 281 246 27 HindIII A'AGCT_T Cuts at: 748 2466 5079 6291 9248 748 Size: 1718 2613 1212 2957 5625 Fragments arranged by size: 5625 2957 2613 1718 1212 HpaI GTT'AAC Cuts at: 11715 11715 Size: 14125 KpnI G_GTAC'C Cuts at: 3756 11450 3756 Size: 7694 6431 MfeI C'AATT_G Cuts at: 5939 11724 5939 Size: 5785 8340 MluI A'CGCG_T Cuts at: 14001 14001 Size: 14125 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5566 6021 6732 7922 8303 10318 10458 11031 Size: 4261 455 711 1190 381 2015 140 573 Cuts at: 11031 11145 13239 13423 1305 Size: 114 2094 184 2007 Fragments arranged by size: 4261 2094 2015 2007 1190 711 573 455 381 184 140 114 MscI TGG'CCA Cuts at: 10 64 1489 1832 3536 5198 5377 5840 6584 Size: 54 1425 343 1704 1662 179 463 744 Cuts at: 6584 7031 8475 10928 11036 11153 10 Size: 447 1444 2453 108 117 2982 Fragments arranged by size: 2982 2453 1704 1662 1444 1425 744 463 447 343 179 117 108 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 4553 5068 5259 6446 9227 9241 Size: 648 1215 2172 515 191 1187 2781 14 Cuts at: 9241 10772 11042 12083 12442 12601 518 Size: 1531 270 1041 359 159 2042 Fragments arranged by size: 2781 2172 2042 1531 1215 1187 1041 648 515 359 270 191 159 14 MspA1I CmG'CkG Cuts at: 1606 2179 2251 2690 2807 3215 3540 3713 4149 Size: 573 72 439 117 408 325 173 436 Cuts at: 4149 4163 4484 4543 6361 7276 7391 7545 7848 Size: 14 321 59 1818 915 115 154 303 Cuts at: 7848 8136 8242 8809 8860 9566 10041 10247 11541 Size: 288 106 567 51 706 475 206 1294 Cuts at: 11541 12218 13366 13611 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1818 1294 1148 915 706 677 573 567 475 439 436 408 325 321 303 288 245 206 173 154 117 115 106 72 59 51 14 NaeI GCC'GGC Cuts at: 9153 9556 9153 Size: 403 13722 NcoI C'CATG_G Cuts at: 513 1065 2004 3633 3861 5406 10743 11088 11148 Size: 552 939 1629 228 1545 5337 345 60 Cuts at: 11148 513 Size: 3490 Fragments arranged by size: 5337 3490 1629 1545 939 552 345 228 60 NdeI CA'TA_TG Cuts at: 387 387 Size: 14125 NgoMIV G'CCGG_C Cuts at: 9151 9554 9151 Size: 403 13722 NheI G'CTAG_C Cuts at: 1052 8431 1052 Size: 7379 6746 NotI GC'GGCC_GC Cuts at: 11572 11572 Size: 14125 NsiI A_TGCA'T Cuts at: 4899 4899 Size: 14125 NspI r_CATG'y Cuts at: 1132 1229 4775 6321 6611 6952 10777 11225 11483 Size: 97 3546 1546 290 341 3825 448 258 Cuts at: 11483 13028 1132 Size: 1545 2229 Fragments arranged by size: 3825 3546 2229 1546 1545 448 341 290 258 97 PasI CC'CwG_GG Cuts at: 1348 4761 5383 1348 Size: 3413 622 10090 Fragments arranged by size: 10090 3413 622 PciI A'CATG_T Cuts at: 4771 6317 11221 13024 4771 Size: 1546 4904 1803 5872 Fragments arranged by size: 5872 4904 1803 1546 PflMI CCAn_nnn'nTGG Cuts at: 1108 3769 5464 8242 11032 11199 1108 Size: 2661 1695 2778 2790 167 4034 Fragments arranged by size: 4034 2790 2778 2661 1695 167 PfoI T'CCnGG_A Cuts at: 2670 5463 5479 8115 8940 9318 9974 11094 2670 Size: 2793 16 2636 825 378 656 1120 5701 Fragments arranged by size: 5701 2793 2636 1120 825 656 378 16 PmeI GTTT'AAAC Cuts at: 11429 11429 Size: 14125 PmlI CAC'GTG Cuts at: 4357 8260 9227 11157 4357 Size: 3903 967 1930 7325 Fragments arranged by size: 7325 3903 1930 967 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4558 4590 4704 4736 6143 6175 12194 12226 13707 Size: 32 114 32 1407 32 6019 32 1481 Cuts at: 13707 13739 4558 Size: 32 4944 Fragments arranged by size: 6019 4944 1481 1407 114 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3651 4317 4344 5648 9483 10071 3651 Size: 666 27 1304 3835 588 7705 Fragments arranged by size: 7705 3835 1304 666 588 27 PshAI GACnn'nnGTC Cuts at: 1613 3188 1613 Size: 1575 12550 PsiI TTA'TAA Cuts at: 4818 6287 11695 4818 Size: 1469 5408 7248 Fragments arranged by size: 7248 5408 1469 PspOMI G'GGCC_C Cuts at: 5394 8667 11136 5394 Size: 3273 2469 8383 Fragments arranged by size: 8383 3273 2469 PspXI vC'TCGA_Gb Cuts at: 2501 2501 Size: 14125 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3066 3098 3066 Size: 32 14093 PstI C_TGCA'G Cuts at: 830 2689 2976 3052 3516 3780 3908 4514 4542 Size: 1859 287 76 464 264 128 606 28 Cuts at: 4542 5850 6627 6777 8587 8859 9073 9111 9798 Size: 1308 777 150 1810 272 214 38 687 Cuts at: 9798 10831 11477 830 Size: 1033 646 3478 Fragments arranged by size: 3478 1859 1810 1308 1033 777 687 646 606 464 287 272 264 214 150 128 76 38 28 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 14125 PvuII CAG'CTG Cuts at: 2690 3540 3713 4149 4163 4484 4543 6361 7276 Size: 850 173 436 14 321 59 1818 915 Cuts at: 7276 7391 8860 9566 10247 2690 Size: 115 1469 706 681 6568 Fragments arranged by size: 6568 1818 1469 915 850 706 681 436 321 173 115 59 14 SacI G_AGCT'C Cuts at: 2508 7095 11319 2508 Size: 4587 4224 5314 Fragments arranged by size: 5314 4587 4224 SalI G'TCGA_C Cuts at: 1936 8021 11467 1936 Size: 6085 3446 4594 Fragments arranged by size: 6085 4594 3446 SanDI GG'GwC_CC Cuts at: 3651 4317 3651 Size: 666 13459 SapI GCTCTTCn'nnn_ Cuts at: 3152 5171 5426 3152 Size: 2019 255 11851 Fragments arranged by size: 11851 2019 255 SbfI CC_TGCA'GG Cuts at: 11477 11477 Size: 14125 ScaI AGT'ACT Cuts at: 7156 12373 14085 7156 Size: 5217 1712 7196 Fragments arranged by size: 7196 5217 1712 SexAI A'CCwGG_T Cuts at: 2583 2613 8327 8682 9620 10221 2583 Size: 30 5714 355 938 601 6487 Fragments arranged by size: 6487 5714 938 601 355 30 SfcI C'TryA_G Cuts at: 826 1045 2685 2860 2972 3048 3512 3776 3904 Size: 219 1640 175 112 76 464 264 128 Cuts at: 3904 4510 4522 4538 5846 6623 6773 8583 8855 Size: 606 12 16 1308 777 150 1810 272 Cuts at: 8855 9069 9107 9759 9794 10827 11293 11473 12608 Size: 214 38 652 35 1033 466 180 1135 Cuts at: 12608 13289 13480 826 Size: 681 191 1471 Fragments arranged by size: 1810 1640 1471 1308 1135 1033 777 681 652 606 466 464 272 264 219 214 191 180 175 150 128 112 76 38 35 16 12 SfiI GGCCn_nnn'nGGCC Cuts at: 9509 9509 Size: 14125 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 14125 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 14125 SmaI CCC'GGG Cuts at: 1872 8666 11452 1872 Size: 6794 2786 4545 Fragments arranged by size: 6794 4545 2786 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 2501 2839 3586 8443 8768 Size: 197 926 102 456 338 747 4857 325 Cuts at: 8768 10025 11345 12229 13130 13392 820 Size: 1257 1320 884 901 262 1553 Fragments arranged by size: 4857 1553 1320 1257 926 901 884 747 456 338 325 262 197 102 SnaBI TAC'GTA Cuts at: 493 11916 493 Size: 11423 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 14125 SphI G_CATG'C Cuts at: 1132 11483 1132 Size: 10351 3774 SrfI GCCC'GGGC Cuts at: 8666 8666 Size: 14125 SspI AAT'ATT Cuts at: 5 52 2855 9700 12049 5 Size: 47 2803 6845 2349 2081 Fragments arranged by size: 6845 2803 2349 2081 47 StuI AGG'CCT Cuts at: 1517 2033 2608 2759 3659 3845 10641 1517 Size: 516 575 151 900 186 6796 5001 Fragments arranged by size: 6796 5001 900 575 516 186 151 StyI C'CwwG_G Cuts at: 513 1065 2004 2489 2679 3348 3633 3861 4828 Size: 552 939 485 190 669 285 228 967 Cuts at: 4828 5406 5808 5878 6031 6889 8449 9123 9412 Size: 578 402 70 153 858 1560 674 289 Cuts at: 9412 10255 10743 11088 11148 11564 513 Size: 843 488 345 60 416 3074 Fragments arranged by size: 3074 1560 967 939 858 843 674 669 578 552 488 485 416 402 345 289 285 228 190 153 70 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6104 6113 7496 9277 9722 9970 12152 Size: 1255 4524 9 1383 1781 445 248 2182 Cuts at: 12152 12169 12322 12507 12952 325 Size: 17 153 185 445 1498 Fragments arranged by size: 4524 2182 1781 1498 1383 1255 445 445 248 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4478 6303 7121 Size: 274 80 33 51 1443 2501 1825 818 Cuts at: 7121 7154 7804 10032 10776 12371 14083 96 Size: 33 650 2228 744 1595 1712 138 Fragments arranged by size: 2501 2228 1825 1712 1595 1443 818 744 650 274 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2533 2565 2805 2837 4026 4058 4080 4112 4149 Size: 32 240 32 1189 32 22 32 37 Cuts at: 4149 4181 7571 7603 8897 8929 9037 9069 14085 Size: 32 3390 32 1294 32 108 32 5016 Cuts at: 14085 14117 2533 Size: 32 2541 Fragments arranged by size: 5016 3390 2541 1294 1189 240 108 37 32 32 32 32 32 32 32 32 32 22 Tth111I GACn'n_nGTC Cuts at: 1316 7581 8329 11102 1316 Size: 6265 748 2773 4339 Fragments arranged by size: 6265 4339 2773 748 XbaI T'CTAG_A Cuts at: 5627 10948 11461 5627 Size: 5321 513 8291 Fragments arranged by size: 8291 5321 513 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 5115 9520 10750 2011 Size: 3104 4405 1230 5386 Fragments arranged by size: 5386 4405 3104 1230 XhoI C'TCGA_G Cuts at: 1943 2501 1943 Size: 558 13567 XmaI C'CCGG_G Cuts at: 1870 8664 11450 1870 Size: 6794 2786 4545 Fragments arranged by size: 6794 4545 2786 XmnI GAAnn'nnTTC Cuts at: 2033 2391 8052 8124 8396 11267 12254 2033 Size: 358 5661 72 272 2871 987 3904 Fragments arranged by size: 5661 3904 2871 987 358 272 72 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4984 11933 276 Size: 53 83 186 936 3450 6949 2468 Fragments arranged by size: 6949 3450 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BsiWI BstZ17I FseI FspAI KasI NarI NruI PacI RsrII SacII SfoI SwaI