(Linear) (Six-base) MAPSORT of: KIEE4296.seq Check: 2515 from: 1 to: 9420 KIEE4296.orf With 182 enzymes: SgfI * August 14, 2013 11:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3434 8239 8327 8773 9237 9420 Size: 3434 4805 88 446 464 183 Fragments arranged by size: 4805 3434 464 446 183 88 AatII G_ACGT'C Cuts at: 0 2981 9420 Size: 2981 6439 AccI GT'mk_AC Cuts at: 0 4186 4940 6017 9420 Size: 4186 754 1077 3403 Fragments arranged by size: 4186 3403 1077 754 Acc65I G'GTAC_C Cuts at: 0 1747 9420 Size: 1747 7673 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 790 1128 1421 1703 1827 2169 2458 2607 Size: 790 338 293 282 124 342 289 149 Cuts at: 2607 3347 3451 3745 5078 5293 5899 5923 6106 Size: 740 104 294 1333 215 606 24 183 Cuts at: 6106 6398 8174 8792 9420 Size: 292 1776 618 628 Fragments arranged by size: 1776 1333 790 740 628 618 606 342 338 294 293 292 289 282 215 183 149 124 104 24 AflII C'TTAA_G Cuts at: 0 6438 9420 Size: 6438 2982 AflIII A'CryG_T Cuts at: 0 2766 4312 6254 7219 7221 9216 9420 Size: 2766 1546 1942 965 2 1995 204 Fragments arranged by size: 2766 1995 1942 1546 965 204 2 AhdI GACnn_n'nnGTC Cuts at: 0 411 8838 9420 Size: 411 8427 582 Fragments arranged by size: 8427 582 411 AleI CACnn'nnGTG Cuts at: 0 7222 7236 9037 9420 Size: 7222 14 1801 383 Fragments arranged by size: 7222 1801 383 14 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2437 6560 8335 9420 Size: 2437 4123 1775 1085 Fragments arranged by size: 4123 2437 1775 1085 AlwNI CAG_nnn'CTG Cuts at: 0 174 805 3646 5386 5511 6334 6645 6678 Size: 174 631 2841 1740 125 823 311 33 Cuts at: 6678 8036 8217 9060 9420 Size: 1358 181 843 360 Fragments arranged by size: 2841 1740 1358 843 823 631 360 311 181 174 125 33 ApaI G_GGCC'C Cuts at: 0 3393 6666 9135 9420 Size: 3393 3273 2469 285 Fragments arranged by size: 3393 3273 2469 285 ApaLI G'TGCA_C Cuts at: 0 5985 6925 8182 8719 9420 Size: 5985 940 1257 537 701 Fragments arranged by size: 5985 1257 940 701 537 ApoI r'AATT_y Cuts at: 0 342 352 1930 4541 5018 5193 6060 6458 Size: 342 10 1578 2611 477 175 867 398 Cuts at: 6458 6745 8542 9420 Size: 287 1797 878 Fragments arranged by size: 2611 1797 1578 878 867 477 398 342 287 175 10 AseI AT'TA_AT Cuts at: 0 5126 9420 Size: 5126 4294 AvaI C'yCGr_G Cuts at: 0 496 1159 4155 6568 6659 7801 9420 Size: 496 663 2996 2413 91 1142 1619 Fragments arranged by size: 2996 2413 1619 1142 663 496 91 AvrII C'CTAG_G Cuts at: 0 3873 6444 9420 Size: 3873 2571 2976 Fragments arranged by size: 3873 2976 2571 BamHI G'GATC_C Cuts at: 0 5930 7725 8039 8927 9420 Size: 5930 1795 314 888 493 Fragments arranged by size: 5930 1795 888 493 314 BanI G'GyrC_C Cuts at: 0 526 957 1747 2368 3396 6526 7759 8220 Size: 526 431 790 621 1028 3130 1233 461 Cuts at: 8220 9420 Size: 1200 Fragments arranged by size: 3130 1233 1200 1028 790 621 526 461 431 BanII G_rGCy'C Cuts at: 0 503 1122 2676 3393 5090 5460 5688 6137 Size: 503 619 1554 717 1697 370 228 449 Cuts at: 6137 6666 7456 8340 9135 9314 9420 Size: 529 790 884 795 179 106 Fragments arranged by size: 1697 1554 884 795 790 717 619 529 503 449 370 228 179 106 BbsI GAAGACnn'nnnn_ Cuts at: 0 2441 2756 3076 3949 4204 4375 4799 5258 Size: 2441 315 320 873 255 171 424 459 Cuts at: 5258 6552 7472 8201 9420 Size: 1294 920 729 1219 Fragments arranged by size: 2441 1294 1219 920 873 729 459 424 320 315 255 171 BbvCI CC'TCA_GC Cuts at: 0 140 149 182 236 2140 2332 9420 Size: 140 9 33 54 1904 192 7088 Fragments arranged by size: 7088 1904 192 140 54 33 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 6007 6041 6117 6151 6559 6593 7915 7949 Size: 6007 34 76 34 408 34 1322 34 Cuts at: 7949 9420 Size: 1471 Fragments arranged by size: 6007 1471 1322 408 76 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 3871 9420 Size: 3871 5549 BclI T'GATC_A Cuts at: 0 1965 2540 5468 5774 8081 9420 Size: 1965 575 2928 306 2307 1339 Fragments arranged by size: 2928 2307 1965 1339 575 306 BfrBI ATG'CAT Cuts at: 0 2892 9420 Size: 2892 6528 BglI GCCn_nnn'nGGC Cuts at: 0 998 1837 4612 7504 8743 9420 Size: 998 839 2775 2892 1239 677 Fragments arranged by size: 2892 2775 1239 998 839 677 BglII A'GATC_T Cuts at: 0 5769 9420 Size: 5769 3651 BlpI GC'TnA_GC Cuts at: 0 566 3798 7633 8055 9420 Size: 566 3232 3835 422 1365 Fragments arranged by size: 3835 3232 1365 566 422 Bme1580I G_kGCm'C Cuts at: 0 531 962 2371 3393 3401 3453 5989 6254 Size: 531 431 1409 1022 8 52 2536 265 Cuts at: 6254 6483 6666 6929 7229 7764 8186 8628 8723 Size: 229 183 263 300 535 422 442 95 Cuts at: 8723 9135 9420 Size: 412 285 Fragments arranged by size: 2536 1409 1022 535 531 442 431 422 412 300 285 265 263 229 183 95 52 8 BmgBI CAC'GTC Cuts at: 0 3456 3468 7955 9255 9420 Size: 3456 12 4487 1300 165 Fragments arranged by size: 4487 3456 1300 165 12 BmrI ACTGGGnnnn_n' Cuts at: 0 3097 3682 3966 4185 5043 6075 6306 6695 Size: 3097 585 284 219 858 1032 231 389 Cuts at: 6695 8931 9015 9105 9420 Size: 2236 84 90 315 Fragments arranged by size: 3097 2236 1032 858 585 389 315 284 231 219 90 84 BmtI G_CTAG'C Cuts at: 0 6430 9420 Size: 6430 2990 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1371 5704 5974 8682 9420 Size: 1371 4333 270 2708 738 Fragments arranged by size: 4333 2708 1371 738 270 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 332 1144 1289 1436 1578 4020 4159 5636 Size: 332 812 145 147 142 2442 139 1477 Cuts at: 5636 5780 6203 6618 6802 6888 6958 6980 7297 Size: 144 423 415 184 86 70 22 317 Cuts at: 7297 7943 7953 8006 8314 8383 8659 9262 9420 Size: 646 10 53 308 69 276 603 158 Fragments arranged by size: 2442 1477 812 646 603 423 415 332 317 308 276 184 158 147 145 144 142 139 86 70 69 53 22 10 Bpu10I CC'TnA_GC Cuts at: 0 140 149 182 236 1214 2140 2332 6418 Size: 140 9 33 54 978 926 192 4086 Cuts at: 6418 7168 8305 9420 Size: 750 1137 1115 Fragments arranged by size: 4086 1137 1115 978 926 750 192 140 54 33 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 819 1602 6784 8041 9361 9420 Size: 25 794 783 5182 1257 1320 59 Fragments arranged by size: 5182 1320 1257 794 783 59 25 BsaI GGTCTCn'nnnn_ Cuts at: 0 642 664 2267 6693 6882 8118 9420 Size: 642 22 1603 4426 189 1236 1302 Fragments arranged by size: 4426 1603 1302 1236 642 189 22 BsaAI yAC'GTr Cuts at: 0 2352 6255 7222 8478 9152 9420 Size: 2352 3903 967 1256 674 268 Fragments arranged by size: 3903 2352 1256 967 674 268 BsaBI GATnn'nnATC Cuts at: 0 476 4394 5347 9420 Size: 476 3918 953 4073 Fragments arranged by size: 4073 3918 953 476 BsaHI Gr'CG_yC Cuts at: 0 2978 4109 4984 7928 9420 Size: 2978 1131 875 2944 1492 Fragments arranged by size: 2978 2944 1492 1131 875 BsaWI w'CCGG_w Cuts at: 0 1114 2100 4935 5369 6276 9420 Size: 1114 986 2835 434 907 3144 Fragments arranged by size: 3144 2835 1114 986 907 434 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 528 558 2144 2174 2889 2919 3108 3138 Size: 528 30 1586 30 715 30 189 30 Cuts at: 3138 3952 3982 6892 6922 7984 8014 9420 Size: 814 30 2910 30 1062 30 1406 Fragments arranged by size: 2910 1586 1406 1062 814 715 528 189 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 128 253 1234 1585 2303 3376 4023 5638 Size: 128 125 981 351 718 1073 647 1615 Cuts at: 5638 6206 6870 7030 7033 7036 7462 7906 7927 Size: 568 664 160 3 3 426 444 21 Cuts at: 7927 7930 7933 8413 8608 9319 9322 9420 Size: 3 3 480 195 711 3 98 Fragments arranged by size: 1615 1073 981 718 711 664 647 568 480 444 426 351 195 160 128 125 98 21 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 3342 3997 7557 7812 8323 9420 Size: 3342 655 3560 255 511 1097 Fragments arranged by size: 3560 3342 1097 655 511 255 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 417 2012 3635 4150 4250 4339 5575 Size: 232 185 1595 1623 515 100 89 1236 Cuts at: 5575 6320 6371 6553 6614 6973 7522 7919 8326 Size: 745 51 182 61 359 549 397 407 Cuts at: 8326 9046 9420 Size: 720 374 Fragments arranged by size: 1623 1595 1236 745 720 549 515 407 397 374 359 232 185 182 100 89 61 51 BsiEI CG_ry'CG Cuts at: 0 9140 9318 9420 Size: 9140 178 102 Fragments arranged by size: 9140 178 102 BsiHKAI G_wGCw'C Cuts at: 0 503 878 1325 2717 3054 3297 4020 4996 Size: 503 375 447 1392 337 243 723 976 Cuts at: 4996 5090 5738 5960 5989 6929 7526 8186 8302 Size: 94 648 222 29 940 597 660 116 Cuts at: 8302 8723 9314 9420 Size: 421 591 106 Fragments arranged by size: 1392 976 940 723 660 648 597 591 503 447 421 375 337 243 222 116 106 94 29 BsmI GAATG_Cn' Cuts at: 0 445 2414 4245 6393 7285 9420 Size: 445 1969 1831 2148 892 2135 Fragments arranged by size: 2148 2135 1969 1831 892 445 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2890 6187 7961 9420 Size: 2890 3297 1774 1459 Fragments arranged by size: 3297 2890 1774 1459 Bsp1286I G_dGCh'C Cuts at: 0 503 531 878 962 1122 1325 2371 2676 Size: 503 28 347 84 160 203 1046 305 Cuts at: 2676 2717 3054 3297 3393 3401 3453 4020 4996 Size: 41 337 243 96 8 52 567 976 Cuts at: 4996 5090 5460 5688 5738 5960 5989 6137 6254 Size: 94 370 228 50 222 29 148 117 Cuts at: 6254 6483 6666 6929 7229 7456 7526 7764 8186 Size: 229 183 263 300 227 70 238 422 Cuts at: 8186 8302 8340 8628 8723 9135 9314 9420 Size: 116 38 288 95 412 179 106 Fragments arranged by size: 1046 976 567 503 422 412 370 347 337 305 300 288 263 243 238 229 228 227 222 203 183 179 160 148 117 116 106 96 95 94 84 70 52 50 41 38 29 28 8 BspEI T'CCGG_A Cuts at: 0 2100 5369 6276 9420 Size: 2100 3269 907 3144 Fragments arranged by size: 3269 3144 2100 907 BspHI T'CATG_A Cuts at: 0 1467 2495 3024 4875 6395 8972 9420 Size: 1467 1028 529 1851 1520 2577 448 Fragments arranged by size: 2577 1851 1520 1467 1028 529 448 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 966 1453 2327 3434 4323 8239 8327 8381 Size: 966 487 874 1107 889 3916 88 54 Cuts at: 8381 8773 9237 9420 Size: 392 464 183 Fragments arranged by size: 3916 1107 966 889 874 487 464 392 183 88 54 BsrBI CCG'CTC Cuts at: 0 6524 7806 8862 9420 Size: 6524 1282 1056 558 Fragments arranged by size: 6524 1282 1056 558 BsrDI GCAATG_nn' Cuts at: 0 7162 7538 7679 9420 Size: 7162 376 141 1741 Fragments arranged by size: 7162 1741 376 141 BsrFI r'CCGG_y Cuts at: 0 4611 6585 7146 7549 9374 9420 Size: 4611 1974 561 403 1825 46 Fragments arranged by size: 4611 1974 1825 561 403 46 BsrGI T'GTAC_A Cuts at: 0 2473 5116 8027 8771 9420 Size: 2473 2643 2911 744 649 Fragments arranged by size: 2911 2643 2473 744 649 BssSI C'ACGA_G Cuts at: 0 4209 5788 7432 7774 9420 Size: 4209 1579 1644 342 1646 Fragments arranged by size: 4209 1646 1644 1579 342 BstAPI GCAn_nnn'nTGC Cuts at: 0 1506 3292 8016 9420 Size: 1506 1786 4724 1404 Fragments arranged by size: 4724 1786 1506 1404 BstEII G'GTnAC_C Cuts at: 0 7253 9408 9420 Size: 7253 2155 12 Fragments arranged by size: 7253 2155 12 BstXI CCAn_nnnn'nTGG Cuts at: 0 699 2740 3979 5614 7238 8919 9420 Size: 699 2041 1239 1635 1624 1681 501 Fragments arranged by size: 2041 1681 1635 1624 1239 699 501 BstYI r'GATC_y Cuts at: 0 1524 3237 3756 3869 5769 5930 6797 6813 Size: 1524 1713 519 113 1900 161 867 16 Cuts at: 6813 7032 7295 7725 8039 8098 8201 8927 9420 Size: 219 263 430 314 59 103 726 493 Fragments arranged by size: 1900 1713 1524 867 726 519 493 430 314 263 219 161 113 103 59 16 Bsu36I CC'TnA_GG Cuts at: 0 946 2228 3639 4117 7885 9420 Size: 946 1282 1411 478 3768 1535 Fragments arranged by size: 3768 1535 1411 1282 946 478 BtgI C'CryG_G Cuts at: 0 1489 1628 1856 2216 2849 3401 7301 8267 Size: 1489 139 228 360 633 552 3900 966 Cuts at: 8267 8738 9035 9083 9143 9193 9420 Size: 471 297 48 60 50 227 Fragments arranged by size: 3900 1489 966 633 552 471 360 297 228 227 139 60 50 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 357 9420 Size: 357 9063 BtsI GCAGTG_nn' Cuts at: 0 405 821 1504 1896 1926 2294 2874 3092 Size: 405 416 683 392 30 368 580 218 Cuts at: 3092 3609 3838 4780 5326 5412 6337 6956 7061 Size: 517 229 942 546 86 925 619 105 Cuts at: 7061 8384 8448 8495 9420 Size: 1323 64 47 925 Fragments arranged by size: 1323 942 925 925 683 619 580 546 517 416 405 392 368 229 218 105 86 64 47 30 ClaI AT'CG_AT Cuts at: 0 2264 2546 9420 Size: 2264 282 6874 Fragments arranged by size: 6874 2264 282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 6873 6908 7389 7424 9420 Size: 6873 35 481 35 1996 Fragments arranged by size: 6873 1996 481 35 35 DraI TTT'AAA Cuts at: 0 2696 4292 9420 Size: 2696 1596 5128 Fragments arranged by size: 5128 2696 1596 DraIII CAC_nnn'GTG Cuts at: 0 3151 6924 7857 8454 9420 Size: 3151 3773 933 597 966 Fragments arranged by size: 3773 3151 966 933 597 EaeI y'GGCC_r Cuts at: 0 1230 1529 2846 3191 3269 3370 3833 4577 Size: 1230 299 1317 345 78 101 463 744 Cuts at: 4577 5024 6468 7496 7547 7836 8072 8921 9029 Size: 447 1444 1028 51 289 236 849 108 Cuts at: 9029 9146 9420 Size: 117 274 Fragments arranged by size: 1444 1317 1230 1028 849 744 463 447 345 299 289 274 236 117 108 101 78 51 EarI CTCTTCn'nnn_ Cuts at: 0 1147 1857 3166 3421 3860 5615 5673 5701 Size: 1147 710 1309 255 439 1755 58 28 Cuts at: 5701 5918 6231 6469 7904 9420 Size: 217 313 238 1435 1516 Fragments arranged by size: 1755 1516 1435 1309 1147 710 439 313 255 238 217 58 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 810 1528 2038 2422 4150 8287 9420 Size: 810 718 510 384 1728 4137 1133 Fragments arranged by size: 4137 1728 1133 810 718 510 384 EcoICRI GAG'CTC Cuts at: 0 501 5088 9312 9420 Size: 501 4587 4224 108 Fragments arranged by size: 4587 4224 501 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 332 790 1128 1144 1289 1421 1436 1578 Size: 332 458 338 16 145 132 15 142 Cuts at: 1578 1703 1827 2169 2458 2607 3347 3451 3745 Size: 125 124 342 289 149 740 104 294 Cuts at: 3745 4020 4159 5078 5293 5636 5780 5899 5923 Size: 275 139 919 215 343 144 119 24 Cuts at: 5923 6106 6203 6398 6618 6802 6888 6958 6980 Size: 183 97 195 220 184 86 70 22 Cuts at: 6980 7297 7943 7953 8006 8174 8314 8383 8659 Size: 317 646 10 53 168 140 69 276 Cuts at: 8659 8792 9262 9420 Size: 133 470 158 Fragments arranged by size: 919 740 646 470 458 343 342 338 332 317 294 289 276 275 220 215 195 184 183 168 158 149 145 144 142 140 139 133 132 125 124 119 104 97 86 70 69 53 24 22 16 15 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 1867 3430 7888 9420 Size: 1867 1563 4458 1532 Fragments arranged by size: 4458 1867 1563 1532 EcoO109I rG'GnC_Cy Cuts at: 0 1646 1696 2130 2312 2339 3390 3643 4098 Size: 1646 50 434 182 27 1051 253 455 Cuts at: 4098 6663 6768 7478 8066 9131 9132 9323 9420 Size: 2565 105 710 588 1065 1 191 97 Fragments arranged by size: 2565 1646 1065 1051 710 588 455 434 253 191 182 105 97 50 27 1 EcoRI G'AATT_C Cuts at: 0 4541 5018 9420 Size: 4541 477 4402 Fragments arranged by size: 4541 4402 477 EcoRV GAT'ATC Cuts at: 0 1578 5012 9420 Size: 1578 3434 4408 Fragments arranged by size: 4408 3434 1578 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2224 3513 3966 6220 7344 9420 Size: 2224 1289 453 2254 1124 2076 Fragments arranged by size: 2254 2224 2076 1289 1124 453 HaeII r_GCGC'y Cuts at: 0 1610 4026 8646 9420 Size: 1610 2416 4620 774 Fragments arranged by size: 4620 2416 1610 774 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 397 429 895 927 1065 1097 1345 1358 Size: 397 32 466 32 138 32 248 13 Cuts at: 1358 1377 1390 1816 1848 2008 2040 2889 2921 Size: 19 13 426 32 160 32 849 32 Cuts at: 2921 3669 3701 3970 4002 6757 6789 6798 6830 Size: 748 32 269 32 2755 32 9 32 Cuts at: 6830 7979 7984 8011 8016 8193 8225 8668 8700 Size: 1149 5 27 5 177 32 443 32 Cuts at: 8700 8824 8851 8856 8883 8983 9015 9420 Size: 124 27 5 27 100 32 405 Fragments arranged by size: 2755 1149 849 748 466 443 426 405 397 269 248 177 160 138 124 100 32 32 32 32 32 32 32 32 32 32 32 32 32 27 27 27 19 13 13 9 5 5 5 HincII GTy'rAC Cuts at: 0 5054 5523 6018 7209 7965 7992 9420 Size: 5054 469 495 1191 756 27 1428 Fragments arranged by size: 5054 1428 1191 756 495 469 27 HindIII A'AGCT_T Cuts at: 0 461 3074 4286 7243 9420 Size: 461 2613 1212 2957 2177 Fragments arranged by size: 2957 2613 2177 1212 461 KpnI G_GTAC'C Cuts at: 0 1751 9420 Size: 1751 7669 MfeI C'AATT_G Cuts at: 0 3934 9420 Size: 3934 5486 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3561 4016 4727 5917 6298 8313 8453 9026 Size: 3561 455 711 1190 381 2015 140 573 Cuts at: 9026 9140 9420 Size: 114 280 Fragments arranged by size: 3561 2015 1190 711 573 455 381 280 140 114 MscI TGG'CCA Cuts at: 0 1531 3193 3372 3835 4579 5026 6470 8923 Size: 1531 1662 179 463 744 447 1444 2453 Cuts at: 8923 9031 9148 9420 Size: 108 117 272 Fragments arranged by size: 2453 1662 1531 1444 744 463 447 272 179 117 108 MslI CAynn'nnrTG Cuts at: 0 376 2548 3063 3254 4441 7222 7236 8767 Size: 376 2172 515 191 1187 2781 14 1531 Cuts at: 8767 9037 9420 Size: 270 383 Fragments arranged by size: 2781 2172 1531 1187 515 383 376 270 191 14 MspA1I CmG'CkG Cuts at: 0 174 246 685 802 1210 1535 1708 2144 Size: 174 72 439 117 408 325 173 436 Cuts at: 2144 2158 2479 2538 4356 5271 5386 5540 5843 Size: 14 321 59 1818 915 115 154 303 Cuts at: 5843 6131 6237 6804 6855 7561 8036 8242 9420 Size: 288 106 567 51 706 475 206 1178 Fragments arranged by size: 1818 1178 915 706 567 475 439 436 408 325 321 303 288 206 174 173 154 117 115 106 72 59 51 14 NaeI GCC'GGC Cuts at: 0 7148 7551 9420 Size: 7148 403 1869 Fragments arranged by size: 7148 1869 403 NcoI C'CATG_G Cuts at: 0 1628 1856 3401 8738 9083 9143 9420 Size: 1628 228 1545 5337 345 60 277 Fragments arranged by size: 5337 1628 1545 345 277 228 60 NgoMIV G'CCGG_C Cuts at: 0 7146 7549 9420 Size: 7146 403 1871 Fragments arranged by size: 7146 1871 403 NheI G'CTAG_C Cuts at: 0 6426 9420 Size: 6426 2994 NsiI A_TGCA'T Cuts at: 0 2894 9420 Size: 2894 6526 NspI r_CATG'y Cuts at: 0 2770 4316 4606 4947 8772 9220 9420 Size: 2770 1546 290 341 3825 448 200 Fragments arranged by size: 3825 2770 1546 448 341 290 200 PasI CC'CwG_GG Cuts at: 0 2756 3378 9420 Size: 2756 622 6042 Fragments arranged by size: 6042 2756 622 PciI A'CATG_T Cuts at: 0 2766 4312 9216 9420 Size: 2766 1546 4904 204 Fragments arranged by size: 4904 2766 1546 204 PflMI CCAn_nnn'nTGG Cuts at: 0 1764 3459 6237 9027 9194 9420 Size: 1764 1695 2778 2790 167 226 Fragments arranged by size: 2790 2778 1764 1695 226 167 PfoI T'CCnGG_A Cuts at: 0 665 3458 3474 6110 6935 7313 7969 9089 Size: 665 2793 16 2636 825 378 656 1120 Cuts at: 9089 9420 Size: 331 Fragments arranged by size: 2793 2636 1120 825 665 656 378 331 16 PmlI CAC'GTG Cuts at: 0 2352 6255 7222 9152 9420 Size: 2352 3903 967 1930 268 Fragments arranged by size: 3903 2352 1930 967 268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2553 2585 2699 2731 4138 4170 9420 Size: 2553 32 114 32 1407 32 5250 Fragments arranged by size: 5250 2553 1407 114 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1646 2312 2339 3643 7478 8066 9420 Size: 1646 666 27 1304 3835 588 1354 Fragments arranged by size: 3835 1646 1354 1304 666 588 27 PshAI GACnn'nnGTC Cuts at: 0 1183 9420 Size: 1183 8237 PsiI TTA'TAA Cuts at: 0 2813 4282 9420 Size: 2813 1469 5138 Fragments arranged by size: 5138 2813 1469 PspOMI G'GGCC_C Cuts at: 0 3389 6662 9131 9420 Size: 3389 3273 2469 289 Fragments arranged by size: 3389 3273 2469 289 PspXI vC'TCGA_Gb Cuts at: 0 496 9420 Size: 496 8924 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1061 1093 9420 Size: 1061 32 8327 Fragments arranged by size: 8327 1061 32 PstI C_TGCA'G Cuts at: 0 684 971 1047 1511 1775 1903 2509 2537 Size: 684 287 76 464 264 128 606 28 Cuts at: 2537 3845 4622 4772 6582 6854 7068 7106 7793 Size: 1308 777 150 1810 272 214 38 687 Cuts at: 7793 8826 9420 Size: 1033 594 Fragments arranged by size: 1810 1308 1033 777 687 684 606 594 464 287 272 264 214 150 128 76 38 28 PvuII CAG'CTG Cuts at: 0 685 1535 1708 2144 2158 2479 2538 4356 Size: 685 850 173 436 14 321 59 1818 Cuts at: 4356 5271 5386 6855 7561 8242 9420 Size: 915 115 1469 706 681 1178 Fragments arranged by size: 1818 1469 1178 915 850 706 685 681 436 321 173 115 59 14 SacI G_AGCT'C Cuts at: 0 503 5090 9314 9420 Size: 503 4587 4224 106 Fragments arranged by size: 4587 4224 503 106 SalI G'TCGA_C Cuts at: 0 6016 9420 Size: 6016 3404 SanDI GG'GwC_CC Cuts at: 0 1646 2312 9420 Size: 1646 666 7108 Fragments arranged by size: 7108 1646 666 SapI GCTCTTCn'nnn_ Cuts at: 0 1147 3166 3421 9420 Size: 1147 2019 255 5999 Fragments arranged by size: 5999 2019 1147 255 ScaI AGT'ACT Cuts at: 0 5151 9420 Size: 5151 4269 SexAI A'CCwGG_T Cuts at: 0 578 608 6322 6677 7615 8216 9420 Size: 578 30 5714 355 938 601 1204 Fragments arranged by size: 5714 1204 938 601 578 355 30 SfcI C'TryA_G Cuts at: 0 680 855 967 1043 1507 1771 1899 2505 Size: 680 175 112 76 464 264 128 606 Cuts at: 2505 2517 2533 3841 4618 4768 6578 6850 7064 Size: 12 16 1308 777 150 1810 272 214 Cuts at: 7064 7102 7754 7789 8822 9288 9420 Size: 38 652 35 1033 466 132 Fragments arranged by size: 1810 1308 1033 777 680 652 606 466 464 272 264 214 175 150 132 128 112 76 38 35 16 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 7504 9420 Size: 7504 1916 SmaI CCC'GGG Cuts at: 0 6661 9420 Size: 6661 2759 SmlI C'TyrA_G Cuts at: 0 40 496 834 1581 6438 6763 8020 9340 Size: 40 456 338 747 4857 325 1257 1320 Cuts at: 9340 9420 Size: 80 Fragments arranged by size: 4857 1320 1257 747 456 338 325 80 40 SrfI GCCC'GGGC Cuts at: 0 6661 9420 Size: 6661 2759 SspI AAT'ATT Cuts at: 0 850 7695 9420 Size: 850 6845 1725 Fragments arranged by size: 6845 1725 850 StuI AGG'CCT Cuts at: 0 28 603 754 1654 1840 8636 9420 Size: 28 575 151 900 186 6796 784 Fragments arranged by size: 6796 900 784 575 186 151 28 StyI C'CwwG_G Cuts at: 0 484 674 1343 1628 1856 2823 3401 3803 Size: 484 190 669 285 228 967 578 402 Cuts at: 3803 3873 4026 4884 6444 7118 7407 8250 8738 Size: 70 153 858 1560 674 289 843 488 Cuts at: 8738 9083 9143 9420 Size: 345 60 277 Fragments arranged by size: 1560 967 858 843 674 669 578 488 484 402 345 289 285 277 228 190 153 70 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 4099 4108 5491 7272 7717 7965 9420 Size: 4099 9 1383 1781 445 248 1455 Fragments arranged by size: 4099 1781 1455 1383 445 248 9 TatI w'GTAC_w Cuts at: 0 2473 4298 5116 5149 5799 8027 8771 9420 Size: 2473 1825 818 33 650 2228 744 649 Fragments arranged by size: 2473 2228 1825 818 744 650 649 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 528 560 800 832 2021 2053 2075 2107 Size: 528 32 240 32 1189 32 22 32 Cuts at: 2107 2144 2176 5566 5598 6892 6924 7032 7064 Size: 37 32 3390 32 1294 32 108 32 Cuts at: 7064 9420 Size: 2356 Fragments arranged by size: 3390 2356 1294 1189 528 240 108 37 32 32 32 32 32 32 32 32 22 Tth111I GACn'n_nGTC Cuts at: 0 5576 6324 9097 9420 Size: 5576 748 2773 323 Fragments arranged by size: 5576 2773 748 323 XbaI T'CTAG_A Cuts at: 0 3622 8943 9420 Size: 3622 5321 477 Fragments arranged by size: 5321 3622 477 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3110 7515 8745 9420 Size: 3110 4405 1230 675 Fragments arranged by size: 4405 3110 1230 675 XhoI C'TCGA_G Cuts at: 0 496 9420 Size: 496 8924 XmaI C'CCGG_G Cuts at: 0 6659 9420 Size: 6659 2761 XmnI GAAnn'nnTTC Cuts at: 0 28 386 6047 6119 6391 9262 9420 Size: 28 358 5661 72 272 2871 158 Fragments arranged by size: 5661 2871 358 272 158 72 28 ZraI GAC'GTC Cuts at: 0 2979 9420 Size: 2979 6441 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuII SacI SalI SanDI SapI ScaI SexAI SfcI SfiI SmaI SmlI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AclI AfeI AgeI AloI AscI AsiSI BaeI BbeI BsiWI BssHII BstBI BstZ17I DrdI EagI FseI FspI FspAI HpaI KasI MluI NarI NdeI NotI NruI PacI PmeI PvuI RsrII SacII SbfI SfoI SgfI SgrAI SnaBI SpeI SphI SwaI