(Circular) (Six-base) MAPSORT of: pFN21AE5816.seq Check: 6001 from: 1 to: 7291 pFN21AE5816 7291 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5101 278 Size: 53 83 186 936 3565 2468 Fragments arranged by size: 3565 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4634 1937 Size: 2697 4594 Acc65I G'GTAC_C Cuts at: 4612 4612 Size: 7291 AclI AA'CG_TT Cuts at: 1563 5418 5791 1563 Size: 3855 373 3063 Fragments arranged by size: 3855 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2318 2623 3274 3506 3588 3918 4451 5354 Size: 686 305 651 232 82 330 533 903 Cuts at: 5354 6738 7006 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 903 686 651 533 330 305 268 232 82 AfeI AGC'GCT Cuts at: 2141 2141 Size: 7291 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7094 AflIII A'CryG_T Cuts at: 1203 2097 6190 7167 1203 Size: 894 4093 977 1327 Fragments arranged by size: 4093 1327 977 894 AgeI A'CCGG_T Cuts at: 6122 6122 Size: 7291 AhdI GACnn_n'nnGTC Cuts at: 6020 6020 Size: 7291 AleI CACnn'nnGTG Cuts at: 1166 3983 1166 Size: 2817 4474 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7291 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4052 4084 4052 Size: 32 7259 AlwNI CAG_nnn'CTG Cuts at: 2413 2440 5034 6606 2413 Size: 27 2594 1572 3098 Fragments arranged by size: 3098 2594 1572 27 ApaI G_GGCC'C Cuts at: 3705 3705 Size: 7291 ApaLI G'TGCA_C Cuts at: 2543 2611 5348 6504 2543 Size: 68 2737 1156 3330 Fragments arranged by size: 3330 2737 1156 68 ApoI r'AATT_y Cuts at: 1493 2777 3056 3932 4252 4259 4294 4600 4826 Size: 1284 279 876 320 7 35 306 226 Cuts at: 4826 4978 7087 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1284 876 320 306 279 226 152 35 7 AseI AT'TA_AT Cuts at: 160 1032 5845 160 Size: 872 4813 1606 Fragments arranged by size: 4813 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7291 AvaI C'yCGr_G Cuts at: 1870 1943 2129 4074 4616 1870 Size: 73 186 1945 542 4545 Fragments arranged by size: 4545 1945 542 186 73 BamHI G'GATC_C Cuts at: 2086 4621 4988 2086 Size: 2535 367 4389 Fragments arranged by size: 4389 2535 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 2564 3821 4612 6067 Size: 325 217 627 327 450 1257 791 1455 Cuts at: 6067 618 Size: 1842 Fragments arranged by size: 1842 1455 1257 791 627 450 327 325 217 BanII G_rGCy'C Cuts at: 1391 2739 3155 3633 3705 4610 1391 Size: 1348 416 478 72 905 4072 Fragments arranged by size: 4072 1348 905 478 416 72 BbeI G_GCGC'C Cuts at: 2568 2568 Size: 7291 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2718 4274 928 Size: 971 819 1556 3945 Fragments arranged by size: 3945 1556 971 819 BbvCI CC'TCA_GC Cuts at: 2054 2054 Size: 7291 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2441 2475 5482 5516 1903 Size: 34 504 34 3007 34 3678 Fragments arranged by size: 3678 3007 504 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5183 6393 5183 Size: 1210 6081 BclI T'GATC_A Cuts at: 1548 1548 Size: 7291 BfrBI ATG'CAT Cuts at: 3221 3221 Size: 7291 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3497 5902 136 Size: 107 122 71 631 605 1825 2405 1525 Fragments arranged by size: 2405 1825 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3711 7286 3711 Size: 3575 3716 BlpI GC'TnA_GC Cuts at: 2420 2738 3653 4708 2420 Size: 318 915 1055 5003 Fragments arranged by size: 5003 1055 915 318 Bme1580I G_kGCm'C Cuts at: 1790 2117 2547 2615 3705 5352 6508 1790 Size: 327 430 68 1090 1647 1156 2573 Fragments arranged by size: 2573 1647 1156 1090 430 327 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7291 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5022 5980 442 Size: 416 53 83 399 24 3605 958 1753 Fragments arranged by size: 3605 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7291 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3678 4100 3678 Size: 422 6869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2753 4438 5951 1239 Size: 974 540 1685 1513 2579 Fragments arranged by size: 2579 1685 1513 974 540 Bpu10I CC'TnA_GC Cuts at: 2054 2054 Size: 7291 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 4473 4496 5416 6281 6579 2543 Size: 1930 23 920 865 298 3255 Fragments arranged by size: 3255 1930 920 865 298 23 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5954 882 Size: 617 4455 2219 Fragments arranged by size: 4455 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3403 3511 3523 5082 493 Size: 711 2199 108 12 1559 2702 Fragments arranged by size: 2702 2199 1559 711 108 12 BsaBI GATnn'nnATC Cuts at: 7285 7285 Size: 7291 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2126 2565 5098 5480 Size: 53 83 186 936 593 439 2533 382 Cuts at: 5480 6115 275 Size: 635 1451 Fragments arranged by size: 2533 1451 936 635 593 439 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2181 5724 6122 6396 6543 6954 2181 Size: 3543 398 274 147 411 2518 Fragments arranged by size: 3543 2518 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7066 Fragments arranged by size: 7066 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 2150 2210 3163 3398 4058 1186 Size: 961 3 60 953 235 660 4419 Fragments arranged by size: 4419 961 953 660 235 60 3 BseYI C'CCAG_C Cuts at: 3835 6494 6990 3835 Size: 2659 496 4136 Fragments arranged by size: 4136 2659 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3145 3392 3524 3545 1163 Size: 573 1409 247 132 21 4909 Fragments arranged by size: 4909 1409 573 247 132 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4741 5502 6530 1540 Size: 275 186 2740 761 1028 2301 Fragments arranged by size: 2740 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2274 2547 2615 2739 5352 5437 6508 2274 Size: 273 68 124 2613 85 1071 3057 Fragments arranged by size: 3057 2613 1071 273 124 85 68 BsmI GAATG_Cn' Cuts at: 3223 3371 4802 4895 3223 Size: 148 1431 93 5619 Fragments arranged by size: 5619 1431 148 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2274 2547 2615 2739 3155 3633 Size: 399 327 157 273 68 124 416 478 Cuts at: 3633 3705 4610 5352 5437 6508 1391 Size: 72 905 742 85 1071 2174 Fragments arranged by size: 2174 1071 905 742 478 416 399 327 273 157 124 85 72 68 BspEI T'CCGG_A Cuts at: 2181 6954 2181 Size: 4773 2518 BspHI T'CATG_A Cuts at: 3159 3783 4353 5180 6910 3159 Size: 624 570 827 1730 3540 Fragments arranged by size: 3540 1730 827 624 570 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3429 4129 4646 844 Size: 2585 700 517 3489 Fragments arranged by size: 3489 2585 700 517 BsrBI CCG'CTC Cuts at: 1394 1823 2578 5178 1394 Size: 429 755 2600 3507 Fragments arranged by size: 3507 2600 755 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2611 2816 3666 5786 5960 65 Size: 1183 1363 205 850 2120 174 1396 Fragments arranged by size: 2120 1396 1363 1183 850 205 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 5935 6122 1700 Size: 110 230 3895 187 2869 Fragments arranged by size: 3895 2869 230 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7291 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7291 BssSI C'ACGA_G Cuts at: 5351 6363 5351 Size: 1012 6279 BstAPI GCAn_nnn'nTGC Cuts at: 794 2551 2610 2998 794 Size: 1757 59 388 5087 Fragments arranged by size: 5087 1757 388 59 BstBI TT'CG_AA Cuts at: 6095 6095 Size: 7291 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7291 BstXI CCAn_nnnn'nTGG Cuts at: 3451 3451 Size: 7291 BstYI r'GATC_y Cuts at: 1972 2086 3711 4621 4988 5373 5390 6842 6928 Size: 114 1625 910 367 385 17 1452 86 Cuts at: 6928 6940 7286 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1625 1452 910 385 367 346 114 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3146 4565 3146 Size: 1419 5872 BtgI C'CryG_G Cuts at: 513 1065 2259 3392 3492 3981 513 Size: 552 1194 1133 100 489 3823 Fragments arranged by size: 3823 1194 1133 552 489 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6552 Fragments arranged by size: 6552 683 56 BtsI GCAGTG_nn' Cuts at: 2521 4807 5601 5621 2521 Size: 2286 794 20 4191 Fragments arranged by size: 4191 2286 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7291 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7256 DraI TTT'AAA Cuts at: 2877 3865 4595 2877 Size: 988 730 5573 Fragments arranged by size: 5573 988 730 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6298 809 Size: 507 4982 1802 Fragments arranged by size: 4982 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2340 4738 Size: 54 1425 50 275 18 426 84 2398 Cuts at: 4738 5627 7256 8 Size: 889 1629 43 Fragments arranged by size: 2398 1629 1425 889 426 275 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4738 1537 Size: 275 2926 4090 Fragments arranged by size: 4090 2926 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2853 5221 1353 Size: 1500 2368 3423 Fragments arranged by size: 3423 2368 1500 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3546 5878 6252 6398 387 Size: 1064 138 1957 2332 374 146 1280 Fragments arranged by size: 2332 1957 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2737 2737 Size: 7291 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2318 2623 2753 3274 3506 3588 Size: 393 581 105 305 130 521 232 82 Cuts at: 3588 3918 4438 4451 5354 5951 6738 7006 1239 Size: 330 520 13 903 597 787 268 1524 Fragments arranged by size: 1524 903 787 597 581 521 520 393 330 305 268 232 130 105 82 13 EcoNI CCTnn'n_nnAGG Cuts at: 3583 3583 Size: 7291 EcoO109I rG'GnC_Cy Cuts at: 3702 3702 Size: 7291 EcoRI G'AATT_C Cuts at: 4252 4294 4600 4978 4252 Size: 42 306 378 6565 Fragments arranged by size: 6565 378 306 42 EcoRV GAT'ATC Cuts at: 3739 4450 3739 Size: 711 6580 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3361 3361 Size: 7291 FspI TGC'GCA Cuts at: 2363 5797 2363 Size: 3434 3857 FspAI rTGC'GCAy Cuts at: 2363 2363 Size: 7291 HaeII r_GCGC'y Cuts at: 1678 2041 2143 2413 2568 6438 1678 Size: 363 102 270 155 3870 2531 Fragments arranged by size: 3870 2531 363 270 155 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4469 4501 5932 5964 6006 Size: 32 135 32 2897 32 1431 32 42 Cuts at: 6006 6038 1373 Size: 32 2626 Fragments arranged by size: 2897 2626 1431 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3038 4635 4881 669 Size: 988 281 1100 1597 246 3079 Fragments arranged by size: 3079 1597 1100 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7291 HpaI GTT'AAC Cuts at: 4881 4881 Size: 7291 KasI G'GCGC_C Cuts at: 2564 2564 Size: 7291 KpnI G_GTAC'C Cuts at: 4616 4616 Size: 7291 MfeI C'AATT_G Cuts at: 2996 4890 2996 Size: 1894 5397 MluI A'CGCG_T Cuts at: 7167 7167 Size: 7291 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2931 6405 6589 1305 Size: 1626 3474 184 2007 Fragments arranged by size: 3474 2007 1626 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2342 10 Size: 54 1425 343 510 4959 Fragments arranged by size: 4959 1425 510 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 3449 3983 5249 5608 5767 518 Size: 648 1008 1275 534 1266 359 159 2042 Fragments arranged by size: 2042 1275 1266 1008 648 534 359 159 MspA1I CmG'CkG Cuts at: 1606 2254 2428 2626 3505 4707 5384 6532 6777 Size: 648 174 198 879 1202 677 1148 245 Cuts at: 6777 1606 Size: 2120 Fragments arranged by size: 2120 1202 1148 879 677 648 245 198 174 NaeI GCC'GGC Cuts at: 2042 2042 Size: 7291 NarI GG'CG_CC Cuts at: 2565 2565 Size: 7291 NcoI C'CATG_G Cuts at: 513 1065 3981 513 Size: 552 2916 3823 Fragments arranged by size: 3823 2916 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7291 NgoMIV G'CCGG_C Cuts at: 2040 2040 Size: 7291 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7291 NotI GC'GGCC_GC Cuts at: 4738 4738 Size: 7291 NsiI A_TGCA'T Cuts at: 3223 3223 Size: 7291 NspI r_CATG'y Cuts at: 1132 1229 2035 2101 4006 4249 4649 6194 1132 Size: 97 806 66 1905 243 400 1545 2229 Fragments arranged by size: 2229 1905 1545 806 400 243 97 66 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7291 PciI A'CATG_T Cuts at: 2097 6190 2097 Size: 4093 3198 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7291 PfoI T'CCnGG_A Cuts at: 2082 2145 3314 2082 Size: 63 1169 6059 Fragments arranged by size: 6059 1169 63 PmeI GTTT'AAAC Cuts at: 4595 4595 Size: 7291 PmlI CAC'GTG Cuts at: 3523 3523 Size: 7291 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5360 5392 6873 6905 5360 Size: 32 1481 32 5746 Fragments arranged by size: 5746 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7291 PsiI TTA'TAA Cuts at: 3972 4861 3972 Size: 889 6402 PspOMI G'GGCC_C Cuts at: 3701 3701 Size: 7291 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3935 3967 3935 Size: 32 7259 PstI C_TGCA'G Cuts at: 830 2253 3711 4643 830 Size: 1423 1458 932 3478 Fragments arranged by size: 3478 1458 1423 932 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7291 PvuII CAG'CTG Cuts at: 2254 3505 2254 Size: 1251 6040 SacI G_AGCT'C Cuts at: 2739 2739 Size: 7291 SalI G'TCGA_C Cuts at: 1936 4633 1936 Size: 2697 4594 SbfI CC_TGCA'GG Cuts at: 4643 4643 Size: 7291 ScaI AGT'ACT Cuts at: 5539 7251 5539 Size: 1712 5579 SfcI C'TryA_G Cuts at: 826 1045 2249 3707 4639 5774 6455 6646 826 Size: 219 1204 1458 932 1135 681 191 1471 Fragments arranged by size: 1471 1458 1204 1135 932 681 219 191 SfoI GGC'GCC Cuts at: 2566 2566 Size: 7291 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7291 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7291 SmaI CCC'GGG Cuts at: 1872 4618 1872 Size: 2746 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 4475 4488 5395 6296 6558 Size: 197 926 615 1917 13 907 901 262 Cuts at: 6558 820 Size: 1553 Fragments arranged by size: 1917 1553 926 907 901 615 262 197 13 SnaBI TAC'GTA Cuts at: 493 5082 493 Size: 4589 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7291 SphI G_CATG'C Cuts at: 1132 4249 4649 1132 Size: 3117 400 3774 Fragments arranged by size: 3774 3117 400 SspI AAT'ATT Cuts at: 5 52 5215 5 Size: 47 5163 2081 Fragments arranged by size: 5163 2081 47 StuI AGG'CCT Cuts at: 1517 3087 1517 Size: 1570 5721 StyI C'CwwG_G Cuts at: 513 1065 3981 4730 513 Size: 552 2916 749 3074 Fragments arranged by size: 3074 2916 749 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3243 5318 5335 5488 5673 6118 325 Size: 1255 1663 2075 17 153 185 445 1498 Fragments arranged by size: 2075 1663 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3804 5537 7249 Size: 274 80 33 51 1443 1827 1733 1712 Cuts at: 7249 96 Size: 138 Fragments arranged by size: 1827 1733 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3581 3613 7251 7283 3581 Size: 32 3638 32 3589 Fragments arranged by size: 3638 3589 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2717 4412 1316 Size: 1401 1695 4195 Fragments arranged by size: 4195 1695 1401 XbaI T'CTAG_A Cuts at: 4627 4627 Size: 7291 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7291 XmaI C'CCGG_G Cuts at: 1870 4616 1870 Size: 2746 4545 XmnI GAAnn'nnTTC Cuts at: 5420 5420 Size: 7291 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5099 276 Size: 53 83 186 936 3565 2468 Fragments arranged by size: 3565 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BsiWI BsmBI BstZ17I DraIII FseI NruI PacI PpuMI PspXI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI