Restriction map for
pFN21AE5816
(Linear) (Six-base) MAPSORT of: KIEE5816.seq Check: 2391 from: 1 to: 2586
KIEE5816.orf
With 182 enzymes: SgfI *
July 26, 2013 11:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 618 1269 1501 1583 1913 2446 2586
Size: 313 305 651 232 82 330 533 140
Fragments arranged by size:
651 533 330 313 305 232 140 82
AfeI AGC'GCT
Cuts at: 0 136 2586
Size: 136 2450
AflIII A'CryG_T
Cuts at: 0 92 2586
Size: 92 2494
AleI CACnn'nnGTG
Cuts at: 0 1978 2586
Size: 1978 608
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2047 2079 2586
Size: 2047 32 507
Fragments arranged by size:
2047 507 32
AlwNI CAG_nnn'CTG
Cuts at: 0 408 435 2586
Size: 408 27 2151
Fragments arranged by size:
2151 408 27
ApaI G_GGCC'C
Cuts at: 0 1700 2586
Size: 1700 886
ApaLI G'TGCA_C
Cuts at: 0 538 606 2586
Size: 538 68 1980
Fragments arranged by size:
1980 538 68
ApoI r'AATT_y
Cuts at: 0 772 1051 1927 2247 2254 2289 2586
Size: 772 279 876 320 7 35 297
Fragments arranged by size:
876 772 320 297 279 35 7
AvaI C'yCGr_G
Cuts at: 0 124 2069 2586
Size: 124 1945 517
Fragments arranged by size:
1945 517 124
BamHI G'GATC_C
Cuts at: 0 81 2586
Size: 81 2505
BanI G'GyrC_C
Cuts at: 0 109 559 1816 2586
Size: 109 450 1257 770
Fragments arranged by size:
1257 770 450 109
BanII G_rGCy'C
Cuts at: 0 734 1150 1628 1700 2586
Size: 734 416 478 72 886
Fragments arranged by size:
886 734 478 416 72
BbeI G_GCGC'C
Cuts at: 0 563 2586
Size: 563 2023
BbsI GAAGACnn'nnnn_
Cuts at: 0 713 2269 2586
Size: 713 1556 317
Fragments arranged by size:
1556 713 317
BbvCI CC'TCA_GC
Cuts at: 0 49 2586
Size: 49 2537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 436 470 2586
Size: 436 34 2116
Fragments arranged by size:
2116 436 34
BfrBI ATG'CAT
Cuts at: 0 1216 2586
Size: 1216 1370
BglI GCCn_nnn'nGGC
Cuts at: 0 1492 2586
Size: 1492 1094
BglII A'GATC_T
Cuts at: 0 1706 2586
Size: 1706 880
BlpI GC'TnA_GC
Cuts at: 0 415 733 1648 2586
Size: 415 318 915 938
Fragments arranged by size:
938 915 415 318
Bme1580I G_kGCm'C
Cuts at: 0 112 542 610 1700 2586
Size: 112 430 68 1090 886
Fragments arranged by size:
1090 886 430 112 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1673 2095 2586
Size: 1673 422 491
Fragments arranged by size:
1673 491 422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 748 2433 2586
Size: 208 540 1685 153
Fragments arranged by size:
1685 540 208 153
Bpu10I CC'TnA_GC
Cuts at: 0 49 2586
Size: 49 2537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 2468 2491 2586
Size: 538 1930 23 95
Fragments arranged by size:
1930 538 95 23
BsaAI yAC'GTr
Cuts at: 0 1398 1506 1518 2586
Size: 1398 108 12 1068
Fragments arranged by size:
1398 1068 108 12
BsaHI Gr'CG_yC
Cuts at: 0 121 560 2586
Size: 121 439 2026
Fragments arranged by size:
2026 439 121
BsaWI w'CCGG_w
Cuts at: 0 176 2586
Size: 176 2410
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 145 205 1158 1393 2053 2586
Size: 142 3 60 953 235 660 533
Fragments arranged by size:
953 660 533 235 142 60 3
BseYI C'CCAG_C
Cuts at: 0 1830 2586
Size: 1830 756
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1140 1387 1519 1540 2586
Size: 1140 247 132 21 1046
Fragments arranged by size:
1140 1046 247 132 21
BsiHKAI G_wGCw'C
Cuts at: 0 269 542 610 734 2586
Size: 269 273 68 124 1852
Fragments arranged by size:
1852 273 269 124 68
BsmI GAATG_Cn'
Cuts at: 0 1218 1366 2586
Size: 1218 148 1220
Fragments arranged by size:
1220 1218 148
Bsp1286I G_dGCh'C
Cuts at: 0 112 269 542 610 734 1150 1628 1700
Size: 112 157 273 68 124 416 478 72
Cuts at: 1700 2586
Size: 886
Fragments arranged by size:
886 478 416 273 157 124 112 72
68
BspEI T'CCGG_A
Cuts at: 0 176 2586
Size: 176 2410
BspHI T'CATG_A
Cuts at: 0 1154 1778 2348 2586
Size: 1154 624 570 238
Fragments arranged by size:
1154 624 570 238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1424 2124 2586
Size: 1424 700 462
Fragments arranged by size:
1424 700 462
BsrBI CCG'CTC
Cuts at: 0 573 2586
Size: 573 2013
BsrDI GCAATG_nn'
Cuts at: 0 606 811 1661 2586
Size: 606 205 850 925
Fragments arranged by size:
925 850 606 205
BsrFI r'CCGG_y
Cuts at: 0 35 2586
Size: 35 2551
BstAPI GCAn_nnn'nTGC
Cuts at: 0 546 605 993 2586
Size: 546 59 388 1593
Fragments arranged by size:
1593 546 388 59
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1446 2586
Size: 1446 1140
BstYI r'GATC_y
Cuts at: 0 81 1706 2586
Size: 81 1625 880
Fragments arranged by size:
1625 880 81
Bsu36I CC'TnA_GG
Cuts at: 0 1141 2560 2586
Size: 1141 1419 26
Fragments arranged by size:
1419 1141 26
BtgI C'CryG_G
Cuts at: 0 254 1387 1487 1976 2586
Size: 254 1133 100 489 610
Fragments arranged by size:
1133 610 489 254 100
BtsI GCAGTG_nn'
Cuts at: 0 516 2586
Size: 516 2070
DraI TTT'AAA
Cuts at: 0 872 1860 2586
Size: 872 988 726
Fragments arranged by size:
988 872 726
EaeI y'GGCC_r
Cuts at: 0 251 335 2586
Size: 251 84 2251
Fragments arranged by size:
2251 251 84
EarI CTCTTCn'nnn_
Cuts at: 0 848 2586
Size: 848 1738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1541 2586
Size: 1541 1045
EcoICRI GAG'CTC
Cuts at: 0 732 2586
Size: 732 1854
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 313 618 748 1269 1501 1583 1913
Size: 208 105 305 130 521 232 82 330
Cuts at: 1913 2433 2446 2586
Size: 520 13 140
Fragments arranged by size:
521 520 330 305 232 208 140 130
105 82 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1578 2586
Size: 1578 1008
EcoO109I rG'GnC_Cy
Cuts at: 0 1697 2586
Size: 1697 889
EcoRI G'AATT_C
Cuts at: 0 2247 2289 2586
Size: 2247 42 297
Fragments arranged by size:
2247 297 42
EcoRV GAT'ATC
Cuts at: 0 1734 2445 2586
Size: 1734 711 141
Fragments arranged by size:
1734 711 141
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1356 2586
Size: 1356 1230
FspI TGC'GCA
Cuts at: 0 358 2586
Size: 358 2228
FspAI rTGC'GCAy
Cuts at: 0 358 2586
Size: 358 2228
HaeII r_GCGC'y
Cuts at: 0 36 138 408 563 2586
Size: 36 102 270 155 2023
Fragments arranged by size:
2023 270 155 102 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2464 2496 2586
Size: 2464 32 90
Fragments arranged by size:
2464 90 32
HincII GTy'rAC
Cuts at: 0 1033 2586
Size: 1033 1553
KasI G'GCGC_C
Cuts at: 0 559 2586
Size: 559 2027
MfeI C'AATT_G
Cuts at: 0 991 2586
Size: 991 1595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 2586
Size: 926 1660
MscI TGG'CCA
Cuts at: 0 337 2586
Size: 337 2249
MslI CAynn'nnrTG
Cuts at: 0 169 1444 1978 2586
Size: 169 1275 534 608
Fragments arranged by size:
1275 608 534 169
MspA1I CmG'CkG
Cuts at: 0 249 423 621 1500 2586
Size: 249 174 198 879 1086
Fragments arranged by size:
1086 879 249 198 174
NaeI GCC'GGC
Cuts at: 0 37 2586
Size: 37 2549
NarI GG'CG_CC
Cuts at: 0 560 2586
Size: 560 2026
NcoI C'CATG_G
Cuts at: 0 1976 2586
Size: 1976 610
NgoMIV G'CCGG_C
Cuts at: 0 35 2586
Size: 35 2551
NsiI A_TGCA'T
Cuts at: 0 1218 2586
Size: 1218 1368
NspI r_CATG'y
Cuts at: 0 30 96 2001 2244 2586
Size: 30 66 1905 243 342
Fragments arranged by size:
1905 342 243 66 30
PciI A'CATG_T
Cuts at: 0 92 2586
Size: 92 2494
PfoI T'CCnGG_A
Cuts at: 0 77 140 1309 2586
Size: 77 63 1169 1277
Fragments arranged by size:
1277 1169 77 63
PmlI CAC'GTG
Cuts at: 0 1518 2586
Size: 1518 1068
PsiI TTA'TAA
Cuts at: 0 1967 2586
Size: 1967 619
PspOMI G'GGCC_C
Cuts at: 0 1696 2586
Size: 1696 890
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1930 1962 2586
Size: 1930 32 624
Fragments arranged by size:
1930 624 32
PstI C_TGCA'G
Cuts at: 0 248 1706 2586
Size: 248 1458 880
Fragments arranged by size:
1458 880 248
PvuII CAG'CTG
Cuts at: 0 249 1500 2586
Size: 249 1251 1086
Fragments arranged by size:
1251 1086 249
SacI G_AGCT'C
Cuts at: 0 734 2586
Size: 734 1852
SfcI C'TryA_G
Cuts at: 0 244 1702 2586
Size: 244 1458 884
Fragments arranged by size:
1458 884 244
SfoI GGC'GCC
Cuts at: 0 561 2586
Size: 561 2025
SmlI C'TyrA_G
Cuts at: 0 553 2470 2483 2586
Size: 553 1917 13 103
Fragments arranged by size:
1917 553 103 13
SphI G_CATG'C
Cuts at: 0 2244 2586
Size: 2244 342
StuI AGG'CCT
Cuts at: 0 1082 2586
Size: 1082 1504
StyI C'CwwG_G
Cuts at: 0 1976 2586
Size: 1976 610
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1238 2586
Size: 1238 1348
TatI w'GTAC_w
Cuts at: 0 1799 2586
Size: 1799 787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1576 1608 2586
Size: 1576 32 978
Fragments arranged by size:
1576 978 32
Tth111I GACn'n_nGTC
Cuts at: 0 712 2407 2586
Size: 712 1695 179
Fragments arranged by size:
1695 712 179
Enzymes that do cut:
AcuI AfeI AflIII AleI AloI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BfrBI BglI BglII BlpI Bme1580I BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII KasI
MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PfoI PmlI PsiI PspOMI
PsrI PstI PvuII SacI SfcI SfoI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AscI AseI AsiSI AvrII BaeI BciVI BclI
BmgBI BmrI BmtI BsaI BsaBI BsaXI BsiEI BsiWI
BsmBI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraIII DrdI EagI FseI HindIII HpaI
KpnI MluI NdeI NheI NotI NruI PacI PasI
PflMI PmeI PpiI PpuMI PshAI PspXI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
XbaI XcmI XhoI XmaI XmnI ZraI