(Circular) (Six-base) MAPSORT of: pFN21AE2420.seq Check: 6012 from: 1 to: 10720 pFN21AE2420 10720 bp With 182 enzymes: SgfI * November 2, 2012 18:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7309 7309 Size: 10720 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8530 278 Size: 53 83 186 936 6994 2468 Fragments arranged by size: 6994 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4789 6257 8063 1937 Size: 2852 1468 1806 4594 Fragments arranged by size: 4594 2852 1806 1468 Acc65I G'GTAC_C Cuts at: 3160 8041 3160 Size: 4881 5839 AclI AA'CG_TT Cuts at: 1563 8847 9220 1563 Size: 7284 373 3063 Fragments arranged by size: 7284 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 3713 4119 4736 6011 6213 7130 7616 Size: 597 1484 406 617 1275 202 917 486 Cuts at: 7616 7715 8783 10167 10435 1632 Size: 99 1068 1384 268 1917 Fragments arranged by size: 1917 1484 1384 1275 1068 917 617 597 486 406 268 202 99 AfeI AGC'GCT Cuts at: 2153 2153 Size: 10720 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10523 AflIII A'CryG_T Cuts at: 1203 3755 5893 9619 10596 1203 Size: 2552 2138 3726 977 1327 Fragments arranged by size: 3726 2552 2138 1327 977 AgeI A'CCGG_T Cuts at: 5884 9551 5884 Size: 3667 7053 AhdI GACnn_n'nnGTC Cuts at: 3845 7947 9449 3845 Size: 4102 1502 5116 Fragments arranged by size: 5116 4102 1502 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10720 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 10720 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3930 3962 6810 6842 7671 7703 3930 Size: 32 2848 32 829 32 6947 Fragments arranged by size: 6947 2848 829 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3227 4025 4991 5326 7702 8463 10035 3227 Size: 798 966 335 2376 761 1572 3912 Fragments arranged by size: 3912 2376 1572 966 798 761 335 ApaI G_GGCC'C Cuts at: 4682 4682 Size: 10720 ApaLI G'TGCA_C Cuts at: 8777 9933 8777 Size: 1156 9564 ApoI r'AATT_y Cuts at: 1493 2216 2615 3135 3239 3557 3677 3728 4478 Size: 723 399 520 104 318 120 51 750 Cuts at: 4478 4553 4815 5617 5672 6689 7602 8029 8255 Size: 75 262 802 55 1017 913 427 226 Cuts at: 8255 8407 10516 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1017 913 802 750 723 520 427 399 318 262 226 152 120 104 75 55 51 AseI AT'TA_AT Cuts at: 160 1032 2447 3443 5820 6219 9274 160 Size: 872 1415 996 2377 399 3055 1606 Fragments arranged by size: 3055 2377 1606 1415 996 872 399 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10720 AvaI C'yCGr_G Cuts at: 1870 1943 2123 7434 8045 1870 Size: 73 180 5311 611 4545 Fragments arranged by size: 5311 4545 611 180 73 AvrII C'CTAG_G Cuts at: 6008 6008 Size: 10720 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6037 6070 6037 Size: 33 10687 BamHI G'GATC_C Cuts at: 8050 8417 8050 Size: 367 10353 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3160 5068 7057 8041 9496 Size: 325 217 627 1373 1908 1989 984 1455 Cuts at: 9496 618 Size: 1842 Fragments arranged by size: 1989 1908 1842 1455 1373 984 627 325 217 BanII G_rGCy'C Cuts at: 1391 2124 4248 4682 6789 7684 8039 1391 Size: 733 2124 434 2107 895 355 4072 Fragments arranged by size: 4072 2124 2107 895 733 434 355 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2725 3471 4429 4484 4749 5087 7355 Size: 971 826 746 958 55 265 338 2268 Cuts at: 7355 928 Size: 4293 Fragments arranged by size: 4293 2268 971 958 826 746 338 265 55 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8911 8945 1903 Size: 34 6974 34 3678 Fragments arranged by size: 6974 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6398 8612 9822 6398 Size: 2214 1210 7296 Fragments arranged by size: 7296 2214 1210 BclI T'GATC_A Cuts at: 1548 2644 5395 5863 1548 Size: 1096 2751 468 6405 Fragments arranged by size: 6405 2751 1096 468 BfrBI ATG'CAT Cuts at: 5203 6140 5203 Size: 937 9783 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9331 136 Size: 107 122 71 631 605 7659 1525 Fragments arranged by size: 7659 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6550 7370 7723 10715 6550 Size: 820 353 2992 6555 Fragments arranged by size: 6555 2992 820 353 BlpI GC'TnA_GC Cuts at: 8137 8137 Size: 10720 Bme1580I G_kGCm'C Cuts at: 1790 4682 8781 9937 1790 Size: 2892 4099 1156 2573 Fragments arranged by size: 4099 2892 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3758 7286 1318 Size: 2440 3528 4752 Fragments arranged by size: 4752 3528 2440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2602 5094 8451 Size: 416 53 83 399 24 1185 2492 3357 Cuts at: 8451 9409 442 Size: 958 1753 Fragments arranged by size: 3357 2492 1753 1185 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5922 1056 Size: 4866 5854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4559 7668 9380 1239 Size: 3320 3109 1712 2579 Fragments arranged by size: 3320 3109 2579 1712 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3407 3617 8845 9710 10008 3407 Size: 210 5228 865 298 4119 Fragments arranged by size: 5228 4119 865 298 210 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 9383 882 Size: 617 7884 2219 Fragments arranged by size: 7884 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6230 7898 8511 493 Size: 711 5026 1668 613 2702 Fragments arranged by size: 5026 2702 1668 711 613 BsaBI GATnn'nnATC Cuts at: 6555 7596 10714 6555 Size: 1041 3118 6561 Fragments arranged by size: 6561 3118 1041 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 8527 8909 9544 Size: 53 83 186 936 537 6457 382 635 Cuts at: 9544 275 Size: 1451 Fragments arranged by size: 6457 1451 936 635 537 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5884 7810 9153 9551 9825 9972 10383 5884 Size: 1926 1343 398 274 147 411 6221 Fragments arranged by size: 6221 1926 1343 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3252 3282 3930 3960 5502 Size: 30 165 30 1849 30 648 30 1542 Cuts at: 5502 5532 7410 7440 7671 7701 1178 Size: 30 1878 30 231 30 4197 Fragments arranged by size: 4197 1878 1849 1542 648 231 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2954 3474 3954 7635 1186 Size: 883 885 520 480 3681 4271 Fragments arranged by size: 4271 3681 885 883 520 480 BseYI C'CCAG_C Cuts at: 5594 6277 9923 10419 5594 Size: 683 3646 496 5895 Fragments arranged by size: 5895 3646 683 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 7266 1163 Size: 573 5530 4617 Fragments arranged by size: 5530 4617 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8170 8931 9959 1540 Size: 275 186 6169 761 1028 2301 Fragments arranged by size: 6169 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 4248 4930 7684 8781 8866 9937 2124 Size: 2124 682 2754 1097 85 1071 2907 Fragments arranged by size: 2907 2754 2124 1097 1071 682 85 BsmI GAATG_Cn' Cuts at: 3521 8231 8324 3521 Size: 4710 93 5917 Fragments arranged by size: 5917 4710 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6119 7931 7966 6119 Size: 1812 35 8873 Fragments arranged by size: 8873 1812 35 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 4248 4682 4930 6789 7684 8039 Size: 399 334 2124 434 248 1859 895 355 Cuts at: 8039 8781 8866 9937 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 2124 1859 1071 895 742 434 399 355 334 248 85 BspEI T'CCGG_A Cuts at: 7810 10383 7810 Size: 2573 8147 BspHI T'CATG_A Cuts at: 3703 3913 6720 8609 10339 3703 Size: 210 2807 1889 1730 4084 Fragments arranged by size: 4084 2807 1889 1730 210 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2131 2307 2545 2779 7309 8075 844 Size: 1287 176 238 234 4530 766 3489 Fragments arranged by size: 4530 3489 1287 766 238 234 176 BsrBI CCG'CTC Cuts at: 1394 1823 8607 1394 Size: 429 6784 3507 Fragments arranged by size: 6784 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3963 4197 7913 9215 9389 65 Size: 1183 2715 234 3716 1302 174 1396 Fragments arranged by size: 3716 2715 1396 1302 1183 234 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2130 5884 9364 9551 1700 Size: 110 320 3754 3480 187 2869 Fragments arranged by size: 3754 3480 2869 320 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10720 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10720 BssSI C'ACGA_G Cuts at: 2792 8780 9792 2792 Size: 5988 1012 3720 Fragments arranged by size: 5988 3720 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3800 7324 794 Size: 3006 3524 4190 Fragments arranged by size: 4190 3524 3006 BstBI TT'CG_AA Cuts at: 7606 9524 7606 Size: 1918 8802 BstEII G'GTnAC_C Cuts at: 1184 4778 1184 Size: 3594 7126 BstXI CCAn_nnnn'nTGG Cuts at: 7513 7513 Size: 10720 BstYI r'GATC_y Cuts at: 1972 3592 4498 4988 5701 6550 7370 7420 7723 Size: 1620 906 490 713 849 820 50 303 Cuts at: 7723 7777 8050 8417 8802 8819 10271 10357 10369 Size: 54 273 367 385 17 1452 86 12 Cuts at: 10369 10715 1972 Size: 346 1977 Fragments arranged by size: 1977 1620 1452 906 849 820 713 490 385 367 346 303 273 86 54 50 17 12 Bsu36I CC'TnA_GG Cuts at: 3179 3966 3179 Size: 787 9933 BtgI C'CryG_G Cuts at: 513 1065 2004 4216 513 Size: 552 939 2212 7017 Fragments arranged by size: 7017 2212 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 7232 487 Size: 683 56 6006 3975 Fragments arranged by size: 6006 3975 683 56 BtsI GCAGTG_nn' Cuts at: 2553 7165 7577 8236 9030 9050 2553 Size: 4612 412 659 794 20 4223 Fragments arranged by size: 4612 4223 794 659 412 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10720 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10685 DraI TTT'AAA Cuts at: 2688 3642 4333 5457 5928 7408 8024 2688 Size: 954 691 1124 471 1480 616 5384 Fragments arranged by size: 5384 1480 1124 954 691 616 471 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7259 9727 809 Size: 507 5943 2468 1802 Fragments arranged by size: 5943 2468 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 5789 7530 Size: 54 1425 50 275 18 244 3715 1741 Cuts at: 7530 8167 9056 10685 8 Size: 637 889 1629 43 Fragments arranged by size: 3715 1741 1629 1425 889 637 275 244 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8167 1537 Size: 275 6355 4090 Fragments arranged by size: 6355 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2557 3488 5821 6030 7143 7359 8650 1353 Size: 1204 931 2333 209 1113 216 1291 3423 Fragments arranged by size: 3423 2333 1291 1204 1113 931 216 209 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 2079 9307 9681 9827 387 Size: 1064 138 441 49 7228 374 146 1280 Fragments arranged by size: 7228 1280 1064 441 374 146 138 49 EcoICRI GAG'CTC Cuts at: 2122 4246 7682 2122 Size: 2124 3436 5160 Fragments arranged by size: 5160 3436 2124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 3713 4119 4559 4736 6011 6213 Size: 393 597 1484 406 440 177 1275 202 Cuts at: 6213 7130 7616 7668 7715 8783 9380 10167 10435 Size: 917 486 52 47 1068 597 787 268 Cuts at: 10435 1239 Size: 1524 Fragments arranged by size: 1524 1484 1275 1068 917 787 597 597 486 440 406 393 268 202 177 52 47 EcoO109I rG'GnC_Cy Cuts at: 2037 3785 4678 4679 5684 6065 6537 7627 2037 Size: 1748 893 1 1005 381 472 1090 5130 Fragments arranged by size: 5130 1748 1090 1005 893 472 381 1 EcoRI G'AATT_C Cuts at: 3239 6689 7602 8029 8407 3239 Size: 3450 913 427 378 5552 Fragments arranged by size: 5552 3450 913 427 378 EcoRV GAT'ATC Cuts at: 3531 3531 Size: 10720 FspI TGC'GCA Cuts at: 9226 9226 Size: 10720 HaeII r_GCGC'y Cuts at: 1678 2155 9867 1678 Size: 477 7712 2531 Fragments arranged by size: 7712 2531 477 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3831 3863 4067 4099 5149 Size: 32 135 32 2259 32 204 32 1050 Cuts at: 5149 5181 5387 5419 6887 6919 7171 7203 7410 Size: 32 206 32 1468 32 252 32 207 Cuts at: 7410 7442 7933 7965 9361 9393 9435 9467 1373 Size: 32 491 32 1396 32 42 32 2626 Fragments arranged by size: 2626 2259 1468 1396 1050 491 252 207 206 204 135 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3175 4304 4571 4790 7446 8064 Size: 988 281 1237 1129 267 219 2656 618 Cuts at: 8064 8310 669 Size: 246 3079 Fragments arranged by size: 3079 2656 1237 1129 988 618 281 267 246 219 HindIII A'AGCT_T Cuts at: 748 4199 748 Size: 3451 7269 HpaI GTT'AAC Cuts at: 7446 8310 7446 Size: 864 9856 KpnI G_GTAC'C Cuts at: 3164 8045 3164 Size: 4881 5839 MfeI C'AATT_G Cuts at: 3510 3999 5589 7004 8319 3510 Size: 489 1590 1415 1315 5911 Fragments arranged by size: 5911 1590 1415 1315 489 MluI A'CGCG_T Cuts at: 10596 10596 Size: 10720 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2722 4495 5581 9834 10018 1305 Size: 1417 1773 1086 4253 184 2007 Fragments arranged by size: 4253 2007 1773 1417 1086 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5791 10 Size: 54 1425 343 3959 4939 Fragments arranged by size: 4939 3959 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5169 5813 6242 8678 9037 9196 518 Size: 648 4003 644 429 2436 359 159 2042 Fragments arranged by size: 4003 2436 2042 648 644 429 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 3227 3844 6506 8136 8813 9961 10206 Size: 406 1215 617 2662 1630 677 1148 245 Cuts at: 10206 1606 Size: 2120 Fragments arranged by size: 2662 2120 1630 1215 1148 677 617 406 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4216 513 Size: 552 939 2212 7017 Fragments arranged by size: 7017 2212 939 552 NdeI CA'TA_TG Cuts at: 387 3799 387 Size: 3412 7308 NheI G'CTAG_C Cuts at: 1052 5918 1052 Size: 4866 5854 NotI GC'GGCC_GC Cuts at: 8167 8167 Size: 10720 NsiI A_TGCA'T Cuts at: 5205 6142 5205 Size: 937 9783 NspI r_CATG'y Cuts at: 1132 1229 2392 3202 5897 8078 9623 1132 Size: 97 1163 810 2695 2181 1545 2229 Fragments arranged by size: 2695 2229 2181 1545 1163 810 97 PasI CC'CwG_GG Cuts at: 1348 3789 1348 Size: 2441 8279 PciI A'CATG_T Cuts at: 5893 9619 5893 Size: 3726 6994 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 10720 PfoI T'CCnGG_A Cuts at: 4204 5689 6748 4204 Size: 1485 1059 8176 Fragments arranged by size: 8176 1485 1059 PmeI GTTT'AAAC Cuts at: 8024 8024 Size: 10720 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3930 3962 7671 7703 8789 8821 10302 10334 3930 Size: 32 3709 32 1086 32 1481 32 4316 Fragments arranged by size: 4316 3709 1481 1086 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3785 5684 6065 6537 3785 Size: 1899 381 472 7968 Fragments arranged by size: 7968 1899 472 381 PshAI GACnn'nnGTC Cuts at: 1613 6542 1613 Size: 4929 5791 PsiI TTA'TAA Cuts at: 4710 5522 8003 8290 4710 Size: 812 2481 287 7140 Fragments arranged by size: 7140 2481 812 287 PspOMI G'GGCC_C Cuts at: 4678 4678 Size: 10720 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5301 5333 7008 7040 5301 Size: 32 1675 32 8981 Fragments arranged by size: 8981 1675 32 32 PstI C_TGCA'G Cuts at: 830 2142 2776 8072 830 Size: 1312 634 5296 3478 Fragments arranged by size: 5296 3478 1312 634 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10720 PvuII CAG'CTG Cuts at: 3227 3844 6506 3227 Size: 617 2662 7441 Fragments arranged by size: 7441 2662 617 SacI G_AGCT'C Cuts at: 2124 4248 7684 2124 Size: 2124 3436 5160 Fragments arranged by size: 5160 3436 2124 SalI G'TCGA_C Cuts at: 1936 4788 8062 1936 Size: 2852 3274 4594 Fragments arranged by size: 4594 3274 2852 SbfI CC_TGCA'GG Cuts at: 8072 8072 Size: 10720 ScaI AGT'ACT Cuts at: 3005 6763 8968 10680 3005 Size: 3758 2205 1712 3045 Fragments arranged by size: 3758 3045 2205 1712 SexAI A'CCwGG_T Cuts at: 4774 4774 Size: 10720 SfcI C'TryA_G Cuts at: 826 1045 2138 2303 2772 3933 5318 8068 9203 Size: 219 1093 165 469 1161 1385 2750 1135 Cuts at: 9203 9884 10075 826 Size: 681 191 1471 Fragments arranged by size: 2750 1471 1385 1161 1135 1093 681 469 219 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10720 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10720 SmaI CCC'GGG Cuts at: 1872 8047 1872 Size: 6175 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3386 3596 7434 8824 9725 9987 Size: 197 926 1443 210 3838 1390 901 262 Cuts at: 9987 820 Size: 1553 Fragments arranged by size: 3838 1553 1443 1390 926 901 262 210 197 SnaBI TAC'GTA Cuts at: 493 7898 8511 493 Size: 7405 613 2702 Fragments arranged by size: 7405 2702 613 SpeI A'CTAG_T Cuts at: 152 4573 152 Size: 4421 6299 SphI G_CATG'C Cuts at: 1132 8078 1132 Size: 6946 3774 SspI AAT'ATT Cuts at: 5 52 2399 3124 4348 5011 5127 6421 6770 Size: 47 2347 725 1224 663 116 1294 349 Cuts at: 6770 7033 8644 5 Size: 263 1611 2081 Fragments arranged by size: 2347 2081 1611 1294 1224 725 663 349 263 116 47 StuI AGG'CCT Cuts at: 1517 3412 4132 1517 Size: 1895 720 8105 Fragments arranged by size: 8105 1895 720 StyI C'CwwG_G Cuts at: 513 1065 2004 4216 4674 6008 6540 7306 8159 Size: 552 939 2212 458 1334 532 766 853 Cuts at: 8159 513 Size: 3074 Fragments arranged by size: 3074 2212 1334 939 853 766 552 532 458 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2299 8747 8764 8917 9102 9547 325 Size: 1255 719 6448 17 153 185 445 1498 Fragments arranged by size: 6448 1498 1255 719 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2088 3003 3479 Size: 274 80 33 51 1443 111 915 476 Cuts at: 3479 5485 6761 7133 8966 10678 96 Size: 2006 1276 372 1833 1712 138 Fragments arranged by size: 2006 1833 1712 1443 1276 915 476 372 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3520 3552 5176 5208 6675 6707 10680 10712 3520 Size: 32 1624 32 1467 32 3973 32 3528 Fragments arranged by size: 3973 3528 1624 1467 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10720 XbaI T'CTAG_A Cuts at: 8056 8056 Size: 10720 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6538 6538 Size: 10720 XhoI C'TCGA_G Cuts at: 1943 7434 1943 Size: 5491 5229 XmaI C'CCGG_G Cuts at: 1870 8045 1870 Size: 6175 4545 XmnI GAAnn'nnTTC Cuts at: 2916 4347 7074 8849 2916 Size: 1431 2727 1775 4787 Fragments arranged by size: 4787 2727 1775 1431 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8528 276 Size: 53 83 186 936 6994 2468 Fragments arranged by size: 6994 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI