################################## # NO CUT ENZYMES ( 193 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AlwI 255 GGATC GGATC(4/5) AlwNI 430 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 397 GTGCTC G[AT]GC[AT]^C BbsI 457 GTCTTC GAAGAC(2/6) BciVI 143 GTATCC GTATCC(6/5) BfaI 334 CTAG C^TAG BpuAI 457 GTCTTC GAAGAC(2/6) BsaJI 147 CCAAGG C^C[ACGT][ACGT]GG BsiHKAI 397 GTGCTC G[AT]GC[AT]^C BsrI 207 CCAGT ACTGG(1/-1) BssKI 245 CCTGG ^CC[ACGT]GG BstAPI 522 GCACAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 245 CCTGG CC^[AT]GG BstXI 476 CCATCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 256 GATC ^GATC EarI 136 GAAGAG CTCTTC(1/4) EcoRII 245 CCTGG ^CC[AT]GG EcoT14I 147 CCAAGG C^C[AT][AT]GG HphI 323 TCACC GGTGA(8/7) Ksp632I 136 GAAGAG CTCTTC(1/4) MaeI 334 CTAG C^TAG MaeII 338 ACGT A^CGT MboI 256 GATC ^GATC MspA1I 314 CCGCTG C[AC]G^C[GT]G MvaI 245 CCTGG CC^[AT]GG NdeI 7 CATATG CA^TATG NdeII 256 GATC ^GATC NlaIV 373 GGCTCC GG[ACGT]^[ACGT]CC PstI 317 CTGCAG CTGCA^G Sau3AI 256 GATC ^GATC ScrFI 245 CCTGG CC^[ACGT]GG SfcI 317 CTGCAG C^T[GA][CT]AG StyI 147 CCAAGG C^C[AT][AT]GG Tsp509I 354 AATT ^AATT TspRI 381 CACTG CA[GC]TG[ACGT][ACGT]^ XbaI 333 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 314 CCGC CCGC(-3/-1) 423 GCGG AluI 294 AGCT AG^CT 526 AGCT AvaII 204 GGTCC G^G[AT]CC 242 GGTCC BmyI 80 GGGCAC G[AGT]GC[ACT]^C 397 GTGCTC BsiYI 408 CCTGCATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 462 CCCTCGAATGG BslI 408 CCTGCATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 462 CCCTCGAATGG Bsp1286I 80 GGGCAC G[AGT]GC[ACT]^C 397 GTGCTC BspMI 301 GCAGGT ACCTGC(4/8) 451 GCAGGT Cfr13I 204 GGTCC G^G[ACGT]CC 242 GGTCC MaeIII 322 GTCAC ^GT[ACGT]AC 426 GTCAC MboII 136 GAAGA GAAGA(8/7) 458 TCTTC NlaIII 259 CATG CATG^ 412 CATG PleI 223 GAGTC GAGTC(4/5) 552 GAGTC Sau96I 204 GGTCC G^G[ACGT]CC 242 GGTCC Tsp45I 322 GTCAC ^GT[GC]AC 426 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### BbvI BpmI Cac8I Fnu4HI HinfI ItaI MnlI MseI MwoI TaqI TfiI Tru9I TseI TthHB8I