(Linear) (Six-base) MAPSORT of: KIEE5261.seq  Check: 6361  from: 1  to: 558



KIEE5261.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:53  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     292     442     558 
   Size:        292     150     116

  Fragments arranged by size: 

                 292     150     116
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     558 
   Size:         83     475
 
AlwNI CAG_nnn'CTG

Cuts at:      0     435     558 
   Size:        435     123
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     450     558 
   Size:        450     108
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     154     558 
   Size:        154     404
 
Bme1580I G_kGCm'C

Cuts at:      0      84     558 
   Size:         84     474
 
BmgBI CAC'GTC

Cuts at:      0     339     558 
   Size:        339     219
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     267     360     558 
   Size:         47     220      93     198

  Fragments arranged by size: 

                 220     198      93      47
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      20      50     558 
   Size:         20      30     508

  Fragments arranged by size: 

                 508      30      20
 
BsiHKAI G_wGCw'C

Cuts at:      0     401     558 
   Size:        401     157
 
Bsp1286I G_dGCh'C

Cuts at:      0      84     401     558 
   Size:         84     317     157

  Fragments arranged by size: 

                 317     157      84
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     292     442     558 
   Size:        292     150     116

  Fragments arranged by size: 

                 292     150     116
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     528     558 
   Size:        528      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     483     558 
   Size:        483      75
 
BtsI GCAGTG_nn'

Cuts at:      0     380     558 
   Size:        380     178
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     558 
   Size:        131     427
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47      83     267     360     558 
   Size:         47      36     184      93     198

  Fragments arranged by size: 

                 198     184      93      47      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     240     272     479     511     558 
   Size:        240      32     207      32      47

  Fragments arranged by size: 

                 240     207      47      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     515     558 
   Size:        515      43
 
MspA1I CmG'CkG

Cuts at:      0     316     558 
   Size:        316     242
 
NdeI CA'TA_TG

Cuts at:      0       8     558 
   Size:          8     550
 
PfoI T'CCnGG_A

Cuts at:      0     244     558 
   Size:        244     314
 
PstI C_TGCA'G

Cuts at:      0     321     558 
   Size:        321     237
 
SfcI C'TryA_G

Cuts at:      0     317     558 
   Size:        317     241
 
StyI C'CwwG_G

Cuts at:      0     147     558 
   Size:        147     411
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     530     558 
   Size:        530      28
 
XbaI T'CTAG_A

Cuts at:      0     333     558 
   Size:        333     225
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     BbsI    BciVI Bme1580I    BmgBI     BpmI
    BsaXI  BsiHKAI Bsp1286I    BspMI   BstAPI    BstXI     BtsI     EarI
  Eco57MI    Hin4I     MmeI   MspA1I     NdeI     PfoI     PstI     SfcI
     StyI    TaqII     XbaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI