Restriction map for
pFN21AE3876
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# NO CUT ENZYMES ( 137 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstBI BstEII
BstPI BstUI BstXI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 629 GACGTC GACGT^C
AcyI 629 GACGTC G[GA]^CG[CT]C
AflIII 720 ACATGT A^C[GA][CT]GT
Alw44I 137 GTGCAC G^TGCAC
AlwNI 232 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 137 GTGCAC G^TGCAC
BamHI 1119 GGATCC G^GATCC
BbsI 442 GAAGAC GAAGAC(2/6)
BcnI 658 CCCGG CC^[GC]GG
BfaI 406 CTAG C^TAG
BglI 1754 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1724 AGATCT A^GATCT
BpuAI 442 GAAGAC GAAGAC(2/6)
BsaHI 629 GACGTC G[GA]^CG[CT]C
BsaI 401 GAGACC GGTCTC(1/5)
BseRI 1604 CTCCTC GAGGAG(10/8)
BsgI 795 GTGCAG GTGCAG(16/14)
BsmBI 627 GAGACG CGTCTC(1/5)
BsmI 896 GAATGC GAATGC(1/-1)
BspLU11I 720 ACATGT A^CATGT
BssSI 1412 CACGAG CACGAG(-5/-1)
Bst1107I 1480 GTATAC GTA^TAC
BstZ17I 1480 GTATAC GTA^TAC
Cac8I 863 GCAAGC GC[ACGT]^[ACGT]GC
DraI 1816 TTTAAA TTT^AAA
DraII 654 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoNI 963 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 654 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 746 GAATTC G^AATTC
EcoRV 1705 GATATC GAT^ATC
HapII 659 CCGG C^CGG
Hin1I 629 GACGTC G[GA]^CG[CT]C
HindIII 129 AAGCTT A^AGCTT
HpaII 659 CCGG C^CGG
MaeI 406 CTAG C^TAG
MslI 1245 CACAATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 659 CCGG C^CGG
NciI 658 CCCGG CC^[GC]GG
PflMI 292 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 654 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 629 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmlI 1607 CTCAAG C^T[CT][GA]AG
SspI 1189 AATATT AAT^ATT
StuI 879 AGGCCT AGG^CCT
TaqI 1133 TCGA T^CGA
Tsp45I 717 GTCAC ^GT[GC]AC
TthHB8I 1133 TCGA T^CGA
Van91I 292 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 1480 GTATAC GT^[AC][GT]AC
1659 GTCTAC
Asp700I 412 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
746 GAATTCCTTC
AspHI 137 GTGCAC G[AT]GC[AT]^C
267 GTGCTC
AvaII 655 GGACC G^G[AT]CC
1269 GGTCC
BmyI 137 GTGCAC G[AGT]GC[ACT]^C
267 GTGCTC
BsiHKAI 137 GTGCAC G[AT]GC[AT]^C
267 GTGCTC
Bsp1286I 137 GTGCAC G[AGT]GC[ACT]^C
267 GTGCTC
Cfr13I 655 GGACC G^G[ACGT]CC
1269 GGTCC
EcoT14I 891 CCAAGG C^C[AT][AT]GG
963 CCTTGG
MaeII 537 ACGT A^CGT
630 ACGT
MspA1I 71 CAGCTG C[AC]G^C[GT]G
367 CAGCTG
NspI 311 GCATGT [GA]CATG^[CT]
720 ACATGT
PvuII 71 CAGCTG CAG^CTG
367 CAGCTG
SapI 1081 GAAGAGC GCTCTTC(1/4)
1236 GAAGAGC
Sau96I 655 GGACC G^G[ACGT]CC
1269 GGTCC
ScaI 857 AGTACT AGT^ACT
1711 AGTACT
StyI 891 CCAAGG C^C[AT][AT]GG
963 CCTTGG
XmnI 412 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
746 GAATTCCTTC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI SfaNI SfcI SspBI TfiI
Tru9I TseI Tsp509I TspRI XhoII