################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 855 TCCGGA T^CCGGA AcyI 658 GACGCC G[GA]^CG[CT]C AfaI 954 GTAC GT^AC AlwI 702 GATCC GGATC(4/5) AocI 392 CCTCAGG CC^T[ACGT]AGG BalI 344 TGGCCA TGG^CCA BbsI 709 GTCTTC GAAGAC(2/6) BbvCI 264 CCTCAGC CCTCAGC(-5/-2) BfaI 25 CTAG C^TAG BpuAI 709 GTCTTC GAAGAC(2/6) BsaHI 658 GACGCC G[GA]^CG[CT]C BsaI 889 GAGACC GGTCTC(1/5) BsaWI 855 TCCGGA [AT]^CCGG[AT] BseAI 855 TCCGGA T^CCGGA BseRI 621 CTCCTC GAGGAG(10/8) BsgI 970 GTGCAG GTGCAG(16/14) BsmFI 175 GGGAC GGGAC(10/14) BsmI 153 GCATTC GAATGC(1/-1) BspEI 855 TCCGGA T^CCGGA BspHI 485 TCATGA T^CATGA BsrBI 595 CCGCTC CCGCTC(-3/-3) BsrDI 768 CATTGC GCAATG(2/0) BstEII 162 GGTCACC G^GT[ACGT]ACC BstPI 162 GGTCACC G^GT[ACGT]ACC Bsu36I 392 CCTCAGG CC^T[ACGT]AGG DpnII 702 GATC ^GATC DsaI 794 CCGTGG C^C[GA][CT]GG Eco81I 392 CCTCAGG CC^T[ACGT]AGG EcoNI 186 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 162 GGTCACC G^GT[ACGT]ACC FseI 587 GGCCGGCC GGCCGG^CC Hin1I 658 GACGCC G[GA]^CG[CT]C HinfI 482 GAATC G^A[ACGT]TC MaeI 25 CTAG C^TAG MaeIII 163 GTCAC ^GT[ACGT]AC MboI 702 GATC ^GATC MluNI 344 TGGCCA TGG^CCA MroI 855 TCCGGA T^CCGGA MscI 344 TGGCCA TGG^CCA MseI 65 TTAA T^TAA MslI 963 CATGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 702 GATC ^GATC PflMI 213 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 313 CAGCTG CAG^CTG RcaI 485 TCATGA T^CATGA RsaI 954 GTAC GT^AC SapI 597 GCTCTTC GCTCTTC(1/4) Sau3AI 702 GATC ^GATC SfaNI 366 GCATC GCATC(5/9) TaqI 905 TCGA T^CGA TfiI 482 GAATC G^A[AT]TC Tru9I 65 TTAA T^TAA Tsp45I 163 GTCAC ^GT[GC]AC TthHB8I 905 TCGA T^CGA Van91I 213 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### ApaI 182 GGGCCC GGGCC^C 409 GGGCCC BglI 269 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 583 GCCTGGCCGGC BpmI 529 CTGGAG CTGGAG(16/14) 640 CTGGAG BsaAI 475 TACGTG [CT]AC^GT[GA] 736 TACGTG BsmAI 451 GAGAC GTCTC(1/5) 889 GAGAC BspMI 362 ACCTGC ACCTGC(4/8) 1008 ACCTGC EcoT14I 780 CCTTGG C^C[AT][AT]GG 872 CCTTGG HgaI 324 GACGC GACGC(5/10) 658 GACGC NaeI 588 GCCGGC GCC^GGC 775 GCCGGC NgoMIV 588 GCCGGC G^CCGGC 775 GCCGGC NspI 725 GCATGC [GA]CATG^[CT] 947 GCATGC PpuMI 175 GGGACCT [GA]G^G[AT]CC[CT] 359 AGGACCT SfcI 462 CTACAG C^T[GA][CT]AG 912 CTACAG SmaI 544 CCCGGG CCC^GGG 809 CCCGGG SphI 725 GCATGC GCATG^C 947 GCATGC StyI 780 CCTTGG C^C[AT][AT]GG 872 CCTTGG XmaCI 544 CCCGGG C^CCGGG 809 CCCGGG XmaI 544 CCCGGG C^CCGGG 809 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AluI AlwNI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI TspRI