(Circular) (Six-base) MAPSORT of: pFN21AE5436.seq Check: 1098 from: 1 to: 5737 pFN21AE5436 5737 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3022 3022 Size: 5737 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3547 278 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3080 1937 Size: 1143 4594 Acc65I G'GTAC_C Cuts at: 3058 3058 Size: 5737 AclI AA'CG_TT Cuts at: 1563 3864 4237 1563 Size: 2301 373 3063 Fragments arranged by size: 3063 2301 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 2111 2354 2389 3800 5184 5452 1632 Size: 402 77 243 35 1411 1384 268 1917 Fragments arranged by size: 1917 1411 1384 402 268 243 77 35 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5540 AflIII A'CryG_T Cuts at: 1203 4636 5613 1203 Size: 3433 977 1327 Fragments arranged by size: 3433 1327 977 AgeI A'CCGG_T Cuts at: 4568 4568 Size: 5737 AhdI GACnn_n'nnGTC Cuts at: 4466 4466 Size: 5737 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5737 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5737 AlwNI CAG_nnn'CTG Cuts at: 2111 2323 2368 2475 3480 5052 2111 Size: 212 45 107 1005 1572 2796 Fragments arranged by size: 2796 1572 1005 212 107 45 ApaI G_GGCC'C Cuts at: 2191 2418 2191 Size: 227 5510 ApaLI G'TGCA_C Cuts at: 3794 4950 3794 Size: 1156 4581 ApoI r'AATT_y Cuts at: 1493 3046 3272 3424 5533 1493 Size: 1553 226 152 2109 1697 Fragments arranged by size: 2109 1697 1553 226 152 AseI AT'TA_AT Cuts at: 160 1032 4291 160 Size: 872 3259 1606 Fragments arranged by size: 3259 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 AvaI C'yCGr_G Cuts at: 1870 1943 2177 2549 2814 3062 1870 Size: 73 234 372 265 248 4545 Fragments arranged by size: 4545 372 265 248 234 73 BamHI G'GATC_C Cuts at: 3067 3434 3067 Size: 367 5370 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3058 4513 618 Size: 325 217 627 1271 1455 1842 Fragments arranged by size: 1842 1455 1271 627 325 217 BanII G_rGCy'C Cuts at: 1391 2127 2191 2269 2418 3056 1391 Size: 736 64 78 149 638 4072 Fragments arranged by size: 4072 736 638 149 78 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2707 928 Size: 971 808 3958 Fragments arranged by size: 3958 971 808 BbvCI CC'TCA_GC Cuts at: 2270 2270 Size: 5737 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3928 3962 1903 Size: 34 1991 34 3678 Fragments arranged by size: 3678 1991 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3629 4839 3629 Size: 1210 4527 BclI T'GATC_A Cuts at: 1548 1548 Size: 5737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2280 2594 4348 Size: 107 122 71 631 605 608 314 1754 Cuts at: 4348 136 Size: 1525 Fragments arranged by size: 1754 1525 631 608 605 314 122 107 71 BglII A'GATC_T Cuts at: 5732 5732 Size: 5737 BlpI GC'TnA_GC Cuts at: 3154 3154 Size: 5737 Bme1580I G_kGCm'C Cuts at: 1790 2191 2418 2445 2562 3798 4954 1790 Size: 401 227 27 117 1236 1156 2573 Fragments arranged by size: 2573 1236 1156 401 227 117 27 BmgBI CAC'GTC Cuts at: 1318 2440 1318 Size: 1122 4615 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3468 4426 442 Size: 416 53 83 399 24 2051 958 1753 Fragments arranged by size: 2051 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2555 2666 4397 1239 Size: 1316 111 1731 2579 Fragments arranged by size: 2579 1731 1316 111 Bpu10I CC'TnA_GC Cuts at: 2270 2270 Size: 5737 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3862 4727 5025 3862 Size: 865 298 4574 Fragments arranged by size: 4574 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2888 4400 882 Size: 617 1389 1512 2219 Fragments arranged by size: 2219 1512 1389 617 BsaAI yAC'GTr Cuts at: 493 1204 2482 2743 3528 493 Size: 711 1278 261 785 2702 Fragments arranged by size: 2702 1278 785 711 261 BsaBI GATnn'nnATC Cuts at: 5731 5731 Size: 5737 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2664 3544 3926 4561 Size: 53 83 186 936 1131 880 382 635 Cuts at: 4561 275 Size: 1451 Fragments arranged by size: 1451 1131 936 880 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2860 4170 4568 4842 4989 5400 2860 Size: 1310 398 274 147 411 3197 Fragments arranged by size: 3197 1310 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2429 2459 1178 Size: 30 165 30 1026 30 4456 Fragments arranged by size: 4456 1026 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2617 1186 Size: 1431 4306 BseYI C'CCAG_C Cuts at: 2202 2561 2852 4940 5436 2202 Size: 359 291 2088 496 2503 Fragments arranged by size: 2503 2088 496 359 291 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2996 1163 Size: 573 1260 3904 Fragments arranged by size: 3904 1260 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3187 3948 4976 1540 Size: 275 186 1186 761 1028 2301 Fragments arranged by size: 2301 1186 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3798 3883 4954 3798 Size: 85 1071 4581 Fragments arranged by size: 4581 1071 85 BsmI GAATG_Cn' Cuts at: 2158 3248 3341 2158 Size: 1090 93 4554 Fragments arranged by size: 4554 1090 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2191 2269 2418 2445 2562 3056 Size: 399 337 64 78 149 27 117 494 Cuts at: 3056 3798 3883 4954 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 494 399 337 149 117 85 78 64 27 BspEI T'CCGG_A Cuts at: 2860 5400 2860 Size: 2540 3197 BspHI T'CATG_A Cuts at: 2490 3626 5356 2490 Size: 1136 1730 2871 Fragments arranged by size: 2871 1730 1136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2376 3022 3092 844 Size: 1532 646 70 3489 Fragments arranged by size: 3489 1532 646 70 BsrBI CCG'CTC Cuts at: 1394 1823 2602 3624 1394 Size: 429 779 1022 3507 Fragments arranged by size: 3507 1022 779 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2772 4232 4406 65 Size: 1183 1524 1460 174 1396 Fragments arranged by size: 1524 1460 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 2593 2780 4381 4568 1700 Size: 110 268 515 187 1601 187 2869 Fragments arranged by size: 2869 1601 515 268 187 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5737 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5737 BssSI C'ACGA_G Cuts at: 3797 4809 3797 Size: 1012 4725 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5737 BstBI TT'CG_AA Cuts at: 4541 4541 Size: 5737 BstEII G'GTnAC_C Cuts at: 1184 2167 1184 Size: 983 4754 BstYI r'GATC_y Cuts at: 1972 3067 3434 3819 3836 5288 5374 5386 5732 Size: 1095 367 385 17 1452 86 12 346 Cuts at: 5732 1972 Size: 1977 Fragments arranged by size: 1977 1452 1095 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2398 2398 Size: 5737 BtgI C'CryG_G Cuts at: 513 1065 2799 513 Size: 552 1734 3451 Fragments arranged by size: 3451 1734 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2961 487 Size: 683 56 1735 3263 Fragments arranged by size: 3263 1735 683 56 BtsI GCAGTG_nn' Cuts at: 2562 2984 3253 4047 4067 2562 Size: 422 269 794 20 4232 Fragments arranged by size: 4232 794 422 269 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5737 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5702 DraI TTT'AAA Cuts at: 3041 3041 Size: 5737 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4744 809 Size: 507 3428 1802 Fragments arranged by size: 3428 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 2591 2802 Size: 54 1425 50 275 18 519 242 211 Cuts at: 2802 3184 4073 5702 8 Size: 382 889 1629 43 Fragments arranged by size: 1629 1425 889 519 382 275 242 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3184 1537 Size: 275 1372 4090 Fragments arranged by size: 4090 1372 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2609 2642 2702 3667 1353 Size: 734 522 33 60 965 3423 Fragments arranged by size: 3423 965 734 522 60 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4324 4698 4844 387 Size: 1064 138 2735 374 146 1280 Fragments arranged by size: 2735 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2111 2354 2389 2555 2666 3800 Size: 393 402 77 243 35 166 111 1134 Cuts at: 3800 4397 5184 5452 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1134 787 597 402 393 268 243 166 111 77 35 EcoNI CCTnn'n_nnAGG Cuts at: 2195 2195 Size: 5737 EcoO109I rG'GnC_Cy Cuts at: 2181 2188 2365 2414 2790 2181 Size: 7 177 49 376 5128 Fragments arranged by size: 5128 376 177 49 7 EcoRI G'AATT_C Cuts at: 3046 3424 3046 Size: 378 5359 FseI GG_CCGG'CC Cuts at: 2597 2597 Size: 5737 FspI TGC'GCA Cuts at: 4243 4243 Size: 5737 HaeII r_GCGC'y Cuts at: 1678 4884 1678 Size: 3206 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 4378 4410 4452 Size: 32 135 32 857 32 1917 32 42 Cuts at: 4452 4484 1373 Size: 32 2626 Fragments arranged by size: 2626 1917 857 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3327 669 Size: 988 281 1143 246 3079 Fragments arranged by size: 3079 1143 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5737 HpaI GTT'AAC Cuts at: 3327 3327 Size: 5737 KpnI G_GTAC'C Cuts at: 3062 3062 Size: 5737 MfeI C'AATT_G Cuts at: 3336 3336 Size: 5737 MluI A'CGCG_T Cuts at: 5613 5613 Size: 5737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4851 5035 1305 Size: 3546 184 2007 Fragments arranged by size: 3546 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2351 10 Size: 54 1425 343 519 3396 Fragments arranged by size: 3396 1425 519 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2972 3695 4054 4213 518 Size: 648 1806 723 359 159 2042 Fragments arranged by size: 2042 1806 723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2111 2320 2644 2683 3153 3830 4978 5223 Size: 505 209 324 39 470 677 1148 245 Cuts at: 5223 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 505 470 324 245 209 39 NaeI GCC'GGC Cuts at: 2595 2782 2595 Size: 187 5550 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5185 NdeI CA'TA_TG Cuts at: 387 387 Size: 5737 NgoMIV G'CCGG_C Cuts at: 2593 2780 2593 Size: 187 5550 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5737 NotI GC'GGCC_GC Cuts at: 3184 3184 Size: 5737 NspI r_CATG'y Cuts at: 1132 1229 2734 2956 3095 4640 1132 Size: 97 1505 222 139 1545 2229 Fragments arranged by size: 2229 1545 1505 222 139 97 PasI CC'CwG_GG Cuts at: 1348 2422 1348 Size: 1074 4663 PciI A'CATG_T Cuts at: 4636 4636 Size: 5737 PflMI CCAn_nnn'nTGG Cuts at: 1108 2224 1108 Size: 1116 4621 PfoI T'CCnGG_A Cuts at: 2361 2361 Size: 5737 PmeI GTTT'AAAC Cuts at: 3041 3041 Size: 5737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3806 3838 5319 5351 3806 Size: 32 1481 32 4192 Fragments arranged by size: 4192 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2181 2365 2181 Size: 184 5553 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5737 PsiI TTA'TAA Cuts at: 3307 3307 Size: 5737 PspOMI G'GGCC_C Cuts at: 2187 2414 2187 Size: 227 5510 PstI C_TGCA'G Cuts at: 830 3089 830 Size: 2259 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5737 PvuII CAG'CTG Cuts at: 2320 2320 Size: 5737 SalI G'TCGA_C Cuts at: 1936 3079 1936 Size: 1143 4594 SapI GCTCTTCn'nnn_ Cuts at: 2609 2609 Size: 5737 SbfI CC_TGCA'GG Cuts at: 3089 3089 Size: 5737 ScaI AGT'ACT Cuts at: 3985 5697 3985 Size: 1712 4025 SfcI C'TryA_G Cuts at: 826 1045 2467 2917 3085 4220 4901 5092 826 Size: 219 1422 450 168 1135 681 191 1471 Fragments arranged by size: 1471 1422 1135 681 450 219 191 168 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5737 SmaI CCC'GGG Cuts at: 1872 2551 2816 3064 1872 Size: 679 265 248 4545 Fragments arranged by size: 4545 679 265 248 SmlI C'TyrA_G Cuts at: 820 1017 1943 3841 4742 5004 820 Size: 197 926 1898 901 262 1553 Fragments arranged by size: 1898 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3528 493 Size: 3035 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5737 SphI G_CATG'C Cuts at: 1132 2734 2956 3095 1132 Size: 1602 222 139 3774 Fragments arranged by size: 3774 1602 222 139 SrfI GCCC'GGGC Cuts at: 2816 2816 Size: 5737 SspI AAT'ATT Cuts at: 5 52 3661 5 Size: 47 3609 2081 Fragments arranged by size: 3609 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5737 StyI C'CwwG_G Cuts at: 513 1065 2785 2877 3176 513 Size: 552 1720 92 299 3074 Fragments arranged by size: 3074 1720 552 299 92 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3764 3781 3934 4119 4564 325 Size: 1255 2184 17 153 185 445 1498 Fragments arranged by size: 2184 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3983 5695 96 Size: 274 80 33 51 1443 2006 1712 138 Fragments arranged by size: 2006 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5697 5729 5697 Size: 32 5705 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5737 XbaI T'CTAG_A Cuts at: 3073 3073 Size: 5737 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5737 XmaI C'CCGG_G Cuts at: 1870 2549 2814 3062 1870 Size: 679 265 248 4545 Fragments arranged by size: 4545 679 265 248 XmnI GAAnn'nnTTC Cuts at: 3866 3866 Size: 5737 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3545 276 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SwaI XcmI