Restriction map for
pFN21AE5436
(Linear) (Six-base) MAPSORT of: KIEE5436.seq Check: 4671 from: 1 to: 1032
KIEE5436.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1017 1032
Size: 1017 15
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 106 349 384 1032
Size: 29 77 243 35 648
Fragments arranged by size:
648 243 77 35 29
AlwNI CAG_nnn'CTG
Cuts at: 0 106 318 363 470 1032
Size: 106 212 45 107 562
Fragments arranged by size:
562 212 107 106 45
ApaI G_GGCC'C
Cuts at: 0 186 413 1032
Size: 186 227 619
Fragments arranged by size:
619 227 186
AvaI C'yCGr_G
Cuts at: 0 172 544 809 1032
Size: 172 372 265 223
Fragments arranged by size:
372 265 223 172
BanII G_rGCy'C
Cuts at: 0 122 186 264 413 1032
Size: 122 64 78 149 619
Fragments arranged by size:
619 149 122 78 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 702 1032
Size: 702 330
BbvCI CC'TCA_GC
Cuts at: 0 265 1032
Size: 265 767
BglI GCCn_nnn'nGGC
Cuts at: 0 275 589 1032
Size: 275 314 443
Fragments arranged by size:
443 314 275
Bme1580I G_kGCm'C
Cuts at: 0 186 413 440 557 1032
Size: 186 227 27 117 475
Fragments arranged by size:
475 227 186 117 27
BmgBI CAC'GTC
Cuts at: 0 435 1032
Size: 435 597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 661 1032
Size: 550 111 371
Fragments arranged by size:
550 371 111
Bpu10I CC'TnA_GC
Cuts at: 0 265 1032
Size: 265 767
BsaI GGTCTCn'nnnn_
Cuts at: 0 883 1032
Size: 883 149
BsaAI yAC'GTr
Cuts at: 0 477 738 1032
Size: 477 261 294
Fragments arranged by size:
477 294 261
BsaHI Gr'CG_yC
Cuts at: 0 659 1032
Size: 659 373
BsaWI w'CCGG_w
Cuts at: 0 855 1032
Size: 855 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 424 454 1032
Size: 424 30 578
Fragments arranged by size:
578 424 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 612 1032
Size: 612 420
BseYI C'CCAG_C
Cuts at: 0 197 556 847 1032
Size: 197 359 291 185
Fragments arranged by size:
359 291 197 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1032
Size: 991 41
BsmI GAATG_Cn'
Cuts at: 0 153 1032
Size: 153 879
Bsp1286I G_dGCh'C
Cuts at: 0 122 186 264 413 440 557 1032
Size: 122 64 78 149 27 117 475
Fragments arranged by size:
475 149 122 117 78 64 27
BspEI T'CCGG_A
Cuts at: 0 855 1032
Size: 855 177
BspHI T'CATG_A
Cuts at: 0 485 1032
Size: 485 547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 371 1017 1032
Size: 371 646 15
Fragments arranged by size:
646 371 15
BsrBI CCG'CTC
Cuts at: 0 597 1032
Size: 597 435
BsrDI GCAATG_nn'
Cuts at: 0 767 1032
Size: 767 265
BsrFI r'CCGG_y
Cuts at: 0 73 588 775 1032
Size: 73 515 187 257
Fragments arranged by size:
515 257 187 73
BstEII G'GTnAC_C
Cuts at: 0 162 1032
Size: 162 870
Bsu36I CC'TnA_GG
Cuts at: 0 393 1032
Size: 393 639
BtgI C'CryG_G
Cuts at: 0 794 1032
Size: 794 238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 956 1032
Size: 956 76
BtsI GCAGTG_nn'
Cuts at: 0 557 979 1032
Size: 557 422 53
Fragments arranged by size:
557 422 53
EaeI y'GGCC_r
Cuts at: 0 344 586 797 1032
Size: 344 242 211 235
Fragments arranged by size:
344 242 235 211
EarI CTCTTCn'nnn_
Cuts at: 0 82 604 637 697 1032
Size: 82 522 33 60 335
Fragments arranged by size:
522 335 82 60 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 106 349 384 550 661 1032
Size: 29 77 243 35 166 111 371
Fragments arranged by size:
371 243 166 111 77 35 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 1032
Size: 190 842
EcoO109I rG'GnC_Cy
Cuts at: 0 176 183 360 409 785 1032
Size: 176 7 177 49 376 247
Fragments arranged by size:
376 247 177 176 49 7
FseI GG_CCGG'CC
Cuts at: 0 592 1032
Size: 592 440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 1032
Size: 424 32 576
Fragments arranged by size:
576 424 32
MscI TGG'CCA
Cuts at: 0 346 1032
Size: 346 686
MslI CAynn'nnrTG
Cuts at: 0 967 1032
Size: 967 65
MspA1I CmG'CkG
Cuts at: 0 106 315 639 678 1032
Size: 106 209 324 39 354
Fragments arranged by size:
354 324 209 106 39
NaeI GCC'GGC
Cuts at: 0 590 777 1032
Size: 590 187 255
Fragments arranged by size:
590 255 187
NgoMIV G'CCGG_C
Cuts at: 0 588 775 1032
Size: 588 187 257
Fragments arranged by size:
588 257 187
NspI r_CATG'y
Cuts at: 0 729 951 1032
Size: 729 222 81
Fragments arranged by size:
729 222 81
PasI CC'CwG_GG
Cuts at: 0 417 1032
Size: 417 615
PflMI CCAn_nnn'nTGG
Cuts at: 0 219 1032
Size: 219 813
PfoI T'CCnGG_A
Cuts at: 0 356 1032
Size: 356 676
PpuMI rG'GwC_Cy
Cuts at: 0 176 360 1032
Size: 176 184 672
Fragments arranged by size:
672 184 176
PspOMI G'GGCC_C
Cuts at: 0 182 409 1032
Size: 182 227 623
Fragments arranged by size:
623 227 182
PvuII CAG'CTG
Cuts at: 0 315 1032
Size: 315 717
SapI GCTCTTCn'nnn_
Cuts at: 0 604 1032
Size: 604 428
SfcI C'TryA_G
Cuts at: 0 462 912 1032
Size: 462 450 120
Fragments arranged by size:
462 450 120
SmaI CCC'GGG
Cuts at: 0 546 811 1032
Size: 546 265 221
Fragments arranged by size:
546 265 221
SphI G_CATG'C
Cuts at: 0 729 951 1032
Size: 729 222 81
Fragments arranged by size:
729 222 81
SrfI GCCC'GGGC
Cuts at: 0 811 1032
Size: 811 221
StyI C'CwwG_G
Cuts at: 0 780 872 1032
Size: 780 92 160
Fragments arranged by size:
780 160 92
XmaI C'CCGG_G
Cuts at: 0 544 809 1032
Size: 544 265 223
Fragments arranged by size:
544 265 223
Enzymes that do cut:
AarI AcuI AlwNI ApaI AvaI BanII BbsI BbvCI
BglI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BstEII Bsu36I BtgI
BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I FseI
Hin4I MscI MslI MspA1I NaeI NgoMIV NspI PasI
PflMI PfoI PpuMI PspOMI PvuII SapI SfcI SmaI
SphI SrfI StyI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI BpuEI
BsaBI BsiEI BsiHKAI BsiWI BsmBI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI